DK2154148T3 - Trunkeret L1 protein af human papillomavirus 11 - Google Patents

Trunkeret L1 protein af human papillomavirus 11 Download PDF

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DK2154148T3
DK2154148T3 DK08757380.4T DK08757380T DK2154148T3 DK 2154148 T3 DK2154148 T3 DK 2154148T3 DK 08757380 T DK08757380 T DK 08757380T DK 2154148 T3 DK2154148 T3 DK 2154148T3
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DK08757380.4T
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Jun Zhang
Jin Wang
Chunyan Yang
Ying Gu
Shaowei Li
Ningshao Xia
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Univ Xiamen
Beijing Wantai Biological Pharmacy Entpr Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00Drugs for genital or sexual disorders; Contraceptives
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/12Keratolytics, e.g. wart or anti-corn preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/20Antivirals for DNA viruses
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/20011Papillomaviridae
    • C12N2710/20022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/20011Papillomaviridae
    • C12N2710/20023Virus like particles [VLP]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/20011Papillomaviridae
    • C12N2710/20034Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
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  • Communicable Diseases (AREA)
  • Reproductive Health (AREA)
  • Dermatology (AREA)
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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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Claims (18)

1.
N-terminalt trunkeret HPV11 L1 protein, der mangler netop de første tre aminosyre ved N-terminalen sammenlignet med vildtype HPV11 L1 protein. 2. N-terminalt trunkeret protein ifølge krav 1, repræsenteret ved en sekvens fremsat i SEQ ID NO:2.
3. Polynukleotid, der koder det N-terminale trunkerede protein ifølge ethvert af krav 1-2.
4. Vektor, som udtrykt ved HPV11 L1 proteinet kun omfatter den genetiske information af polynukleotidet ifølge krav 3.
5. Celle omfattende vektoren ifølge krav 4.
6. Sammensætning omfattende det N-terminale trunkerede protein ifølge krav 1 eller 2.
7. HPV11 viruslignende partikel (VLP) omfattende eller bestående af det N-terminalt trunkerede protein ifølge krav 1 eller 2.
8. Fremgangsmåde til fremstilling af det N-terminalt trunkerede HPV11 L1 protein ifølge krav 1 eller 2, omfattende: a) eksprimering af et HPV L1 gen, der koder det N- terminalt trunkerede HPV L1 protein i et E. coli ekspressionssystem, b) opbrydning af den E. coli, som har eksprimeret det N- terminalt trunkerede HPV L1 protein, i en opløsning med en saltkoncentration fra 100mM til 600mM, og isolering af det ovenpå flydende, c) sænkning af saltkoncentrationen i det ovenpå flydende af b) til fra 100 mM til og med 0 ved anvendelse af vand eller en svag saltopløsning, og opsamling af et udfældningsprodukt, d) genopløsning af det udfældede af c) i en opløsning ved en saltkoncentration fra 150mM til 2500 mM, tilførsel af et reduktionsmiddel til det, og derefter isolering af den resulterende opløsning, hvori opløsningen indeholder det N-terminalt trunkerede HPV L1 protein med en renhed på mindst 50%.
9. Vaccine til forebyggelse af condyloma acuminatum eller HPV infektioner, omfattende HPV11 VLP ifølge krav 7 og bærere eller excipienser, som er anvendelige til vacciner.
10. Vaccine til forebyggelse af condyloma acuminatum eller HPV infektioner ifølge krav 9, yderligere omfattende mindst en HPV VLP valgt fra gruppen bestående af VLP’er af HPV type 6, 16, 18, 31, 33, 45, 52 og 58.
11. Vaccine til forebyggelse af condyloma acuminatum eller HPV infektioner ifølge krav 9, omfattende: en HPV 6 VLP omfattende et protein med en aminosyrese-kvens fremsat i SEQ ID NO:7 og en HPV 11 VLP omfattende et protein med en aminosyresekvens fremsat i SEQ ID NO: 1.
12. Vaccine til forebyggelse af condyloma acuminatum eller HPV infektioner ifølge krav 11, yderligere omfattende en HPV 16 VLP omfattende et protein med en aminosyresekvens fremsat i SEQ ID NO:8 og en HPV 18 VLP omfattende et protein med en aminosyresekvens fremsat i SEQ ID NO: 9.
13. Anvendelse af det N-terminalt trunkerede protein ifølge ethvert af krav 1-2 eller VLP ifølge krav 7 til fremstilling af en vaccine til forebyggelse af condyloma acuminatum eller HPV infektioner.
14. Vaccine omfattende det N-terminalt trunkerede HPV 11 L1 protein ifølge ethvert af krav 1-2 eller VLP ifølge krav 7 eller vaccinen ifølge ethvert af krav 9-12 til anvendelse ved forebyggende behandling af et individ med behov for forebyggelse af condyloma acuminatum eller HPV infektioner.
15. Fremgangsmåde ifølge krav 8, yderligere omfattende: e) yderligere rensning af det N-terminalt trunkerede HPV 11 L1 protein til en renhed på mindst 50% ved kromatografi, og f) fjernelse af reduktionsmidlet fra HPV 11 L1 proteinet fremskaffet i e).
16. Fremgangsmåde til fremstilling af en vaccine til forebyggelse af condyloma acuminatum eller HPV infektioner, omfattende blanding af VLP ifølge krav 7 med bærere eller excipienser, som er anvendelige til vacciner.
17.
Fremgangsmåde ifølge krav 16, omfattende blanding af VLP ifølge krav 7 og en eller flere VLP’er af HPV type 6, 16, 18, 31, 33, 45, 52 og 58 med bærere eller excipienser, som er anvendelige til vacciner.
DK08757380.4T 2007-05-29 2008-05-29 Trunkeret L1 protein af human papillomavirus 11 DK2154148T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN200710105763 2007-05-29
PCT/CN2008/001049 WO2008145020A1 (fr) 2007-05-29 2008-05-29 Protéine l1 tronquée du papillomavirus 11 humain

Publications (1)

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DK2154148T3 true DK2154148T3 (da) 2016-09-19

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Application Number Title Priority Date Filing Date
DK08757380.4T DK2154148T3 (da) 2007-05-29 2008-05-29 Trunkeret L1 protein af human papillomavirus 11
DK15160363.6T DK2910566T5 (da) 2007-05-29 2008-05-29 Trunkeret L1 protein af human papillomavirus 11
DK15160399.0T DK2907821T3 (da) 2007-05-29 2008-05-29 Fremgangsmåde til fremstilling af et N-terminal trunkeret L1 protein af human papillomavirus (HPV)

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DK15160363.6T DK2910566T5 (da) 2007-05-29 2008-05-29 Trunkeret L1 protein af human papillomavirus 11
DK15160399.0T DK2907821T3 (da) 2007-05-29 2008-05-29 Fremgangsmåde til fremstilling af et N-terminal trunkeret L1 protein af human papillomavirus (HPV)

Country Status (6)

Country Link
US (3) US9533035B2 (da)
EP (3) EP2907821B1 (da)
CN (1) CN101343314B (da)
BR (1) BRPI0810959B1 (da)
DK (3) DK2154148T3 (da)
WO (1) WO2008145020A1 (da)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9364529B2 (en) 2007-04-29 2016-06-14 Beijing Wantai Biological Pharmacy Enterprise Co., Ltd. Truncated L1 protein of human papillomavirus type 18
US9428555B2 (en) * 2007-04-29 2016-08-30 Beijing Wantai Biological Pharmacy Enterprise Co., Ltd. Truncated L1 protein of Human Papillomavirus type 16
BRPI0810951B8 (pt) * 2007-05-29 2021-05-25 Beijing Wantai Biological Pharmacy Entpr Co Ltd proteína l1 do hpv6, polinucleotídeo, vetor, célula, partícula semelhante ao vírus (vlp) hpv6, método para produzir a proteína hpv6 l1 truncada, vacina para prevenção de condiloma acuminado ou de infecções por hpv, uso da proteína hpv6 l1 truncada, método para prevenção de condiloma acuminado ou infecções por hpv, método para obter uma vlp de uma proteína l1 do hpv6 e método para produzir uma vacina para a prevenção de condiloma acuminado ou de infecções por hpv
EP2589604B1 (en) * 2010-07-02 2016-10-05 Xiamen University Truncated l1 protein of human papillomavirus type 52
CN102229660B (zh) 2011-05-25 2015-04-22 厦门大学 截短的人乳头瘤病毒33型l1蛋白
KR101559622B1 (ko) 2012-07-30 2015-10-13 중앙대학교 산학협력단 인유두종바이러스 바이러스 유사입자의 고효율 정제방법
CN105177025B (zh) * 2013-12-03 2022-11-01 北京康乐卫士生物技术股份有限公司 18型重组人乳头瘤病毒病毒样颗粒及其制备方法
US10513541B2 (en) * 2015-12-04 2019-12-24 Xiamen University Mutant of L1 protein of human papillomavirus type 11
CN106831960B (zh) * 2015-12-04 2019-11-05 厦门大学 一种人乳头瘤病毒6型l1蛋白的突变体
WO2021013069A1 (zh) * 2019-07-19 2021-01-28 神州细胞工程有限公司 嵌合的人乳头瘤病毒11型l1蛋白
CN114539363B (zh) * 2020-11-26 2023-12-01 中国医学科学院基础医学研究所 一种c端改造的人乳头瘤病毒11型l1蛋白及其用途

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69434383T2 (de) 1993-03-09 2005-11-24 The University Of Rochester Herstellung von menschlichem papillomavirus hüllprotein und virus-ähnlichen teilchen
US20020193565A1 (en) * 1998-03-27 2002-12-19 Stanley Margaret Anne Antigen preparation and use
AU3730600A (en) * 1999-03-18 2000-10-04 Xiaojiang Chen Compositions preparations and uses of human papillomavirus l1 protein
AP2004003021A0 (en) 2001-08-31 2004-06-30 Univ Cape Town Vectors, constructs and transgenic plants for HPV-11 and HPV-16 L1 capsid protein.
GB0206359D0 (en) 2002-03-18 2002-05-01 Glaxosmithkline Biolog Sa Viral antigens
WO2003093437A2 (en) 2002-05-02 2003-11-13 University Of Rochester Production of papillomavirus vaccines in plants
CN1293093C (zh) 2002-08-30 2007-01-03 马润林 乳头瘤病毒衣壳蛋白的原核制备和应用
CN1683010A (zh) 2005-02-06 2005-10-19 哈尔滨医科大学 人乳头瘤病毒型双价病毒样颗粒混合蛋白抗原及构建方法
CN101153280B (zh) * 2006-09-29 2015-08-19 厦门大学 从原核生物中纯化人乳头瘤病毒晚期蛋白l1的方法

Also Published As

Publication number Publication date
WO2008145020A1 (fr) 2008-12-04
EP2154148A4 (en) 2011-01-05
US20170065708A1 (en) 2017-03-09
DK2907821T3 (da) 2017-04-10
US9943586B2 (en) 2018-04-17
EP2910566A1 (en) 2015-08-26
HK1208473A1 (en) 2016-03-04
BRPI0810959B1 (pt) 2021-06-29
EP2907821B1 (en) 2016-12-28
US9533035B2 (en) 2017-01-03
US20100291141A1 (en) 2010-11-18
DK2910566T3 (da) 2016-10-24
EP2907821A1 (en) 2015-08-19
EP2154148B1 (en) 2016-06-22
CN101343314A (zh) 2009-01-14
CN101343314B (zh) 2015-11-25
HK1124344A1 (zh) 2009-07-10
BRPI0810959A2 (pt) 2015-07-21
US10537629B2 (en) 2020-01-21
EP2910566B1 (en) 2016-07-13
US20180207257A1 (en) 2018-07-26
DK2910566T5 (da) 2016-11-07
EP2154148A1 (en) 2010-02-17

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