DK2177608T3 - Immobiliseret lipase og fremgangsmåde til fremstilling af den samme - Google Patents

Immobiliseret lipase og fremgangsmåde til fremstilling af den samme Download PDF

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Publication number
DK2177608T3
DK2177608T3 DK08791744.9T DK08791744T DK2177608T3 DK 2177608 T3 DK2177608 T3 DK 2177608T3 DK 08791744 T DK08791744 T DK 08791744T DK 2177608 T3 DK2177608 T3 DK 2177608T3
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DK
Denmark
Prior art keywords
oil
lipase
water emulsion
activity
immobilized lipase
Prior art date
Application number
DK08791744.9T
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English (en)
Inventor
Eiji Iwaoka
Toshiharu Arishima
Original Assignee
Fuji Oil Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Fuji Oil Co Ltd filed Critical Fuji Oil Co Ltd
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Publication of DK2177608T3 publication Critical patent/DK2177608T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (4)

1. Immobiliseret lipasesammensætning, omfattende en olie-i-vand emulsion, som immobiliseres i et bærestof, hvori olie-i-vand emulsionen indeholder et triglycerid, en polymeremulgator og et enzym med lipaseaktivitet, hvori polymeremulgatoren er et polysaccharid, hvori sammensætningen har forbedret transesterificeringsaktivitet sammenlignet med konventionelle immobiliseringsmetoder.
2. Immobiliseret lipasesammensætning ifølge krav 1, hvori polymeremulgatoren er gummi arabicum.
3. Immobiliseret lipasesammensætning ifølge krav 1, hvori polymeremulgatoren er et vandopløseligt sojabønnepolysaccharid.
4. Fremgangsmåde til at fremstille en immobiliseret lipasesammensætning med forbedret transesterificeringsaktivitet sammenlignet med konventionelle fremgangsmåder til immobilisering, omfattende: at præparere en olie-i-vand emulsion indeholdende et triglycerid, en polymeremulgator og et enzym med lipaseaktivitet; at absorbere olie-i-vand emulsionen i et bærestof, efterfulgt af tørring, hvori polymeremulgatoren er et polysaccharid.
DK08791744.9T 2007-07-31 2008-07-28 Immobiliseret lipase og fremgangsmåde til fremstilling af den samme DK2177608T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007199240 2007-07-31
PCT/JP2008/063509 WO2009017087A1 (ja) 2007-07-31 2008-07-28 固定化リパーゼおよびその製造方法

Publications (1)

Publication Number Publication Date
DK2177608T3 true DK2177608T3 (da) 2015-12-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DK08791744.9T DK2177608T3 (da) 2007-07-31 2008-07-28 Immobiliseret lipase og fremgangsmåde til fremstilling af den samme

Country Status (6)

Country Link
US (1) US20100216209A1 (da)
EP (1) EP2177608B1 (da)
JP (1) JP5177143B2 (da)
DK (1) DK2177608T3 (da)
MY (1) MY150945A (da)
WO (1) WO2009017087A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5585454B2 (ja) * 2009-02-04 2014-09-10 不二製油株式会社 乳化粉末リパーゼ製剤の製造方法
US11384218B2 (en) * 2017-08-31 2022-07-12 Carbios Liquid composition comprising biological entities and uses thereof
US20220240556A1 (en) * 2019-09-23 2022-08-04 Conopco, Inc., D/B/A Unilever Oil-in-water emulsion comprising plant protein
JP6847288B1 (ja) * 2019-10-09 2021-03-24 アサヒビール株式会社 容器詰飲料及びその製造方法
WO2022085419A1 (ja) * 2020-10-19 2022-04-28 不二製油グループ本社株式会社 酵素固定化用担体及びそれを用いた固定化酵素

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK402583D0 (da) 1983-09-05 1983-09-05 Novo Industri As Fremgangsmade til fremstilling af et immobiliseret lipasepraeparat og anvendelse deraf
DK153762C (da) 1985-02-27 1989-01-09 Novo Industri As Fremgangsmaade til fremstilling af et immobiliseret lipasepraeparat
JPH066058B2 (ja) 1985-12-07 1994-01-26 不二製油株式会社 酵素剤の製造法
GB8729890D0 (en) * 1987-12-22 1988-02-03 Unilever Plc Improvements in & relating to fat processes
JP2794201B2 (ja) 1989-07-31 1998-09-03 味の素株式会社 固定化リパーゼ酵素剤
US5177013A (en) * 1989-07-31 1993-01-05 Ajinomoto Co., Inc. Preparation of an immobilized lipase having a low water content without drying
DK73592D0 (da) * 1992-06-03 1992-06-03 Novo Nordisk As Nyt enzym
JP3603325B2 (ja) 1994-03-31 2004-12-22 不二製油株式会社 高エステル交換活性酵素剤およびその製造方法
WO1996019118A1 (en) * 1994-12-22 1996-06-27 Kao Corporation Modifier and modifier composition for protein-containing substance
TR199701705T1 (xx) * 1995-06-27 1998-04-21 Unilever N.V. Trigliserid ya�lar�n i�lenmesinde hareketsiz enzim ve kullan�m�.
CN1135265C (zh) * 1996-04-12 2004-01-21 诺沃奇梅兹有限公司 含酶颗粒及其生产方法
JP4017082B2 (ja) * 1996-12-19 2007-12-05 ユニリーバー・ナームローゼ・ベンノートシャープ 固定化酵素及びトリグリセリド油の処理のためのその使用
JP4057157B2 (ja) 1998-08-14 2008-03-05 トタニ技研工業株式会社 プラスチック袋の製造方法
JP3589904B2 (ja) * 1999-06-17 2004-11-17 花王株式会社 酸性水中油型乳化組成物
JP3779505B2 (ja) * 1999-08-24 2006-05-31 花王株式会社 油脂組成物
JP4116844B2 (ja) * 2001-09-13 2008-07-09 花王株式会社 油脂組成物
IL152290A0 (en) * 2002-10-14 2003-05-29 Enzymotec Ltd Immobilization of compounds on polymeric matrix
JP4381037B2 (ja) * 2003-06-04 2009-12-09 花王株式会社 油脂組成物
DE10337451A1 (de) * 2003-08-14 2005-03-17 Cognis Deutschland Gmbh & Co. Kg Verwendung von PIT-Emulsionen in enzymatischen Reaktionen
CA2548723A1 (en) * 2003-12-19 2005-06-19 Syngenta Participations Ag Microbially expressed xylanases and their use as feed additives and other uses
US7601381B2 (en) * 2004-07-13 2009-10-13 Fuji Oil Company, Limited Polysaccharides and protein conjugate, and emulsifiers and emulsions containing it
DE102005002700A1 (de) * 2005-01-19 2006-07-27 Cognis Deutschland Gmbh & Co. Kg Zusammensetzungen verwendbar als Biotreibstoff
US8067052B2 (en) * 2005-02-25 2011-11-29 Siddharth Shastri Nutritional composition and a container for the convenient transport and storage of the nutritional composition
JP5139743B2 (ja) 2007-08-06 2013-02-06 株式会社クボタ 歩行型草刈機

Also Published As

Publication number Publication date
US20100216209A1 (en) 2010-08-26
EP2177608A1 (en) 2010-04-21
EP2177608B1 (en) 2015-10-14
JPWO2009017087A1 (ja) 2010-10-21
WO2009017087A1 (ja) 2009-02-05
JP5177143B2 (ja) 2013-04-03
MY150945A (en) 2014-03-14
EP2177608A4 (en) 2010-11-24

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