DK2201115T4 - Mutanter med evne til at producere 1,4-butandiol og fremgangsmåde til at fremstille 1,4-butandiol ved anvendelse heraf - Google Patents

Mutanter med evne til at producere 1,4-butandiol og fremgangsmåde til at fremstille 1,4-butandiol ved anvendelse heraf Download PDF

Info

Publication number
DK2201115T4
DK2201115T4 DK08793214.1T DK08793214T DK2201115T4 DK 2201115 T4 DK2201115 T4 DK 2201115T4 DK 08793214 T DK08793214 T DK 08793214T DK 2201115 T4 DK2201115 T4 DK 2201115T4
Authority
DK
Denmark
Prior art keywords
mutant
succinate
butanediol
gene
gene encoding
Prior art date
Application number
DK08793214.1T
Other languages
English (en)
Other versions
DK2201115T3 (da
Inventor
Si Jae Park
Sang Hyun Lee
Sang Yup Lee
Eun Jeong Lee
Original Assignee
Lg Chemical Ltd
Korea Advanced Inst Sci & Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40429511&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2201115(T4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lg Chemical Ltd, Korea Advanced Inst Sci & Tech filed Critical Lg Chemical Ltd
Application granted granted Critical
Publication of DK2201115T3 publication Critical patent/DK2201115T3/da
Publication of DK2201115T4 publication Critical patent/DK2201115T4/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/03—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01061—4-Hydroxybutyrate dehydrogenase (1.1.1.61)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01024—Succinate-semialdehyde dehydrogenase (NAD+) (1.2.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
    • C12Y103/99002—Butyryl-CoA dehydrogenase (1.3.99.2), i.e. short chain acyl-CoA dehydrogenase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (14)

1. Mutant, som udviser høj produktion af 1,4-butandiol, og som fremstilles ved at indføre eller amplificere gener, som koder for enzymer, der omdanner succi-nat til 4-hydroxybutyrat og 4-hydroxybutyrat til 1,4-butandiol, i en mikroorganisme i stand til at producere succinat, hvor mikroorganismen i stand til at producere succinat er en bakterie, og hvor genet, som koder for enzymet, som omdanner succinat til 4-hydroxybutyrat, er valgt fra gruppen bestående af gener, som koder for succinyl-CoA-trans-ferase, succinatsemialdehyddehydrogenase, og 4-hydroxyburatdehydrogenase, og genet, som koder for enzymet, der omdanner 4-hydroxybutyrat til 1,4-butandiol er et gen, der koder for phosphotransbutyrylase, et gen, som koder for butyryl-kinase og et gen, som koder for alkoholdehygrogenasereducerende 4-hydroxybutyrat-CoA.
2. Mutant ifølge krav 1, hvor bakterien er valgt fra gruppen bestående af vomflora, Corynebacterium sp., Brevibacterium sp. og E. coli.
3. Mutant ifølge krav 2, hvor vomflorabakterierne har inaktive gener, som koder for i) lactatdehydrogenase (IdhA) og pyruvatformatlyase (ptf), ii) lactatdrhy-drogenase {IhdA), pyruvatformatlyase {Pff), phosphotranacetylase (pta) og ace-tatkinase (ackA), eller iii) lactatdehydrogenase (IhdA), pyruvatformatlyase (Pfl) og phosphopyruvatcarboxylase (Ppc) og producerer succinat i en høj koncentration uden væsentlig produktion i anaerob tilstand af andre organiske syrer.
4. Mutant ifølge krav 2 eller 3, hvor vomflorabakterierne er valgt fra gruppen bestående af Mannheimia sp., Actinobacillus sp. og Anaerobiospirillum sp.
5. Mutant ifølge krav 4, hvor vomflorabakterierne er valgt fra gruppen bestående af Mannheimia succiniciproducens MBEL55E (KCTC 0769BP), og Marrn-heimia sp. LPK (KCTC 10558BP), LPK4 og LPK7 (KCTC 10626BP).
6. Mutant ifølge krav 2, hvor E. coli har inaktive gener, som koder for glucose-phosphotransferase (ptsG) og pyruvatkinase (pykA og pykF) og producerer succinat i høj koncentration uden væsentlig produktion af andre organiske syrer ved anaerobe betingelser.
7. Mutant ifølge krav 6, hvor E. coli-mutanten er W3110GFA.
8. Mutant ifølge krav 1, hvor genet, som koder for enzymet, der omdanner succinat til 4-hydroxybutyrat, stammer fra Clostridium kiuyveri.
9. Mutant ifølge krav 1, hvor genet, som koder for enzymet, der omdanner 4-hydroxybutyrat til 1,4-butandiol, stammer fra Clostridium acetobutylicum.
10. Mutant ifølge krav 1, hvor alkoholdehydrogenasen er butyl-CoA-dehydro-genase stammende fra Clostridium acetobutylicum.
11. Mutant ifølge krav 1, hvor mutanten har et inaktivt gen associeret med omdannelsen af succinatsemialdehyd til succinat.
12. Mutant ifølge krav 11, hvor genet associeret med omdannelse af succinatsemialdehyd til succinat er et gen, som koder for succinsemialdehyddehydro-genase.
13. Mutant ifølge krav 1, hvor et gen, som koder for C4-dicarboxy sent transportprotein (DctA) associeret med transport af succinat, yderligere indføres i eller amplificeres i mutanten.
14. Fremgangsmåde til at fremstille 1,4-butandiol omfattende: at dyrke mutanten ifølge et vilkårligt af kravene 1 til 13 i et medie indeholdende glucose, og opnå 1,4-butandiol fra mediet.
DK08793214.1T 2007-09-07 2008-08-13 Mutanter med evne til at producere 1,4-butandiol og fremgangsmåde til at fremstille 1,4-butandiol ved anvendelse heraf DK2201115T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020070091081A KR101042242B1 (ko) 2007-09-07 2007-09-07 1,4-부탄디올 생성능을 가지는 변이체 및 이를 이용한1,4-부탄디올의 제조방법
PCT/KR2008/004700 WO2009031766A2 (en) 2007-09-07 2008-08-13 Mutants having capability to produce 1,4-butanediol and method for preparing 1,4-butanediol using the same

Publications (2)

Publication Number Publication Date
DK2201115T3 DK2201115T3 (da) 2013-02-11
DK2201115T4 true DK2201115T4 (da) 2016-11-07

Family

ID=40429511

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08793214.1T DK2201115T4 (da) 2007-09-07 2008-08-13 Mutanter med evne til at producere 1,4-butandiol og fremgangsmåde til at fremstille 1,4-butandiol ved anvendelse heraf

Country Status (8)

Country Link
US (2) US9096860B2 (da)
EP (1) EP2201115B2 (da)
KR (1) KR101042242B1 (da)
CN (1) CN101883853B (da)
AU (1) AU2008295788B2 (da)
DK (1) DK2201115T4 (da)
ES (1) ES2396179T5 (da)
WO (1) WO2009031766A2 (da)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0823327A2 (pt) 2007-03-16 2013-10-22 Genomatica Inc Biocatalisadores microbianos que não ocorrem naturalmente e métodos para a biosíntese de ácido 4-hidroxibutanóico e 1,4-butanodiol
US7947483B2 (en) * 2007-08-10 2011-05-24 Genomatica, Inc. Methods and organisms for the growth-coupled production of 1,4-butanediol
CA2712779C (en) 2008-01-22 2021-03-16 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
DK2262901T3 (da) 2008-03-05 2019-01-21 Genomatica Inc Organismer der producerer primær alkohol
EP3514242A3 (en) 2008-09-10 2019-08-28 Genomatica, Inc. Microrganisms for the production of 1,4-butanediol
BRPI1013505A2 (pt) 2009-04-30 2018-02-14 Genomatica Inc organismos para a produção de isopropanol, n-butanol, e isobutanol
MY176050A (en) 2009-04-30 2020-07-22 Genomatica Inc Organisms for the production of 1,3-butanediol
LT2438036T (lt) 2009-06-04 2017-06-26 Genomatica, Inc. Fermentacijos sultinio komponentų išskyrimo būdas
WO2010141920A2 (en) 2009-06-04 2010-12-09 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol and related methods
EP3190174A1 (en) 2009-08-05 2017-07-12 Genomatica, Inc. Semi-synthetic terephthalic acid via microorganisms that produce muconic acid
US8715971B2 (en) * 2009-09-09 2014-05-06 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol and 1,4-butanediol
KR20180014240A (ko) 2009-10-23 2018-02-07 게노마티카 인코포레이티드 아닐린의 제조를 위한 미생물
US8530210B2 (en) 2009-11-25 2013-09-10 Genomatica, Inc. Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone
KR20120120493A (ko) * 2009-12-10 2012-11-01 게노마티카 인코포레이티드 합성 가스 또는 기타 가스상 탄소원 및 메탄올을 1,3-부탄디올로 변환하는 방법 및 변환용 유기체
JP2013517796A (ja) 2010-01-29 2013-05-20 ジェノマティカ・インコーポレイテッド ｐ−トルイル酸およびテレフタル酸を生合成するための方法および微生物
MX2012009134A (es) 2010-02-11 2012-11-30 Metabolix Inc Proceso para producir componente monomerico de biomasa de polihidroxialcanoato geneticamente modificado.
US9023636B2 (en) 2010-04-30 2015-05-05 Genomatica, Inc. Microorganisms and methods for the biosynthesis of propylene
PH12013500158A1 (en) 2010-07-26 2013-03-11 Genomatica Inc Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene
CN102191237A (zh) * 2011-03-25 2011-09-21 福建省麦丹生物集团有限公司 一种增强l-苯丙氨酸合成代谢途径的方法
US20140114082A1 (en) 2011-06-08 2014-04-24 Metabolix, Inc. Biorefinery Process For THF Production
US9353386B2 (en) * 2011-06-15 2016-05-31 B.R.A.I.N. Biotechnology Research And Information Network Ag Means and methods for producing propanediol
WO2012177943A1 (en) 2011-06-22 2012-12-27 Genomatica, Inc. Microorganisms for producing 1,4-butanediol and methods related thereto
BR112014002859A2 (pt) 2011-08-10 2017-06-13 Metabolix Inc purificação pós-processamento para produção de gama-butiro lactona
WO2013085361A2 (ko) * 2011-12-07 2013-06-13 한국과학기술원 4-하이드록시부티릭산 고생성능을 가지는 변이 미생물 및 이를 이용한 4-하이드록시부티릭산의 제조방법
KR20130094115A (ko) * 2012-02-15 2013-08-23 삼성전자주식회사 1,4-부탄디올의 생산을 위한 변형 미생물
SI2855687T1 (sl) 2012-06-04 2020-09-30 Genomatica, Inc. Mikroorganizmi in metode za proizvodnjo 4-hidroksibutirat, 1,4-butandiola in sorodne spojine
KR20140014648A (ko) 2012-07-25 2014-02-06 삼성전자주식회사 1,4-부탄디올의 고효율 생산을 위한 변형 미생물
KR102023618B1 (ko) 2012-07-27 2019-09-20 삼성전자주식회사 1,4-ｂｄｏ 생성능이 개선된 변이 미생물 및 이를 이용한 1,4-ｂｄｏ의 제조방법
US9657316B2 (en) 2012-08-27 2017-05-23 Genomatica, Inc. Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,4-butanediol related thereto
US9932611B2 (en) 2012-10-22 2018-04-03 Genomatica, Inc. Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing succinate related thereto
WO2014099725A1 (en) 2012-12-17 2014-06-26 Genomatica, Inc. Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing adipate, 6-aminocaproate, hexamethylenediamine or caprolactam related thereto
WO2014152665A1 (en) 2013-03-15 2014-09-25 Genomatica, Inc. Process and systems for obtaining 1,4-butanediol from fermentation broths
KR101581504B1 (ko) 2013-03-18 2015-12-31 지에스칼텍스 주식회사 2,3-부탄디올의 생성능이 증강된 재조합 미생물 및 이를 이용한 2,3-부탄디올의 생산 방법
EP2818551B1 (en) * 2013-06-28 2017-04-05 Samsung Electronics Co., Ltd Corynebacterium, in which a gene encoding CoA independent succinate semialdehyde dehydrogenase and a gene encoding L-lactate dehydrogenase are attenatued and method of producing C4 compounds, e.g. 4-hydroxybutyrate
KR102097065B1 (ko) 2013-08-23 2020-04-03 삼성전자주식회사 4-히드록시부티레이트 생산 균주 및 이를 이용한 4-히드록시부티레이트의 혐기적 생산 방법
KR101748930B1 (ko) * 2015-02-09 2017-06-20 지에스칼텍스 주식회사 다이올 생산용 재조합 미생물
CN104926930B (zh) * 2015-06-30 2018-02-06 西南大学 出芽短梗霉二羧酸转运蛋白及其重组载体和应用
CA3151149C (en) * 2015-10-13 2024-03-26 Lanzatech Nz, Inc. Genetically engineered bacterium comprising energy-generating fermentation pathway
CN105647953A (zh) * 2016-03-18 2016-06-08 黑龙江大学 高产2,3-丁二醇的产酸克雷伯氏基因工程菌株的构建方法及其发酵方法
CN112877269B (zh) 2020-01-15 2021-12-24 中国科学院天津工业生物技术研究所 生产赖氨酸的微生物以及赖氨酸的生产方法
KR20240051254A (ko) * 2021-08-31 2024-04-19 게노마티카 인코포레이티드 포르메이트 데하이드로게나제 변이체 및 이용 방법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998036078A1 (en) * 1997-02-13 1998-08-20 James Madison University Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units
GB0219062D0 (en) * 2002-08-15 2002-09-25 Rockwool Int Method and environment for growing plants
KR100630819B1 (ko) * 2004-04-23 2006-10-02 한국과학기술원 신규 루멘 박테리아 변이균주 및 이를 이용한 숙신산의제조방법
JP4672671B2 (ja) * 2003-11-27 2011-04-20 コリア アドバンスド インスティチュート オブ サイエンス アンド テクノロジィ 新規ルーメンバクテリア変異菌株及びそれを用いたコハク酸の製造方法
GB0409704D0 (en) * 2004-04-30 2004-06-02 Nokia Corp A method for verifying a first identity and a second identity of an entity
KR20060001134A (ko) * 2004-06-30 2006-01-06 삼성전기주식회사 고주파 ｉｄ 리더간 통신 기능을 갖는 고주파 ｉｄ 리더
KR100656590B1 (ko) 2004-07-30 2006-12-11 한국과학기술원 박테리아 변이균주 및 이를 이용한 숙신산 및 아미노산의제조방법
KR20070096348A (ko) 2006-03-23 2007-10-02 주식회사 엘지화학 1,4―ｂｕｔａｎｅｄｉｏｌ〔１，４―ＢＤＯ〕생성능을가지는 변이체 및 이를 이용한 １，４―ＢＤＯ의 제조방법
US7947483B2 (en) * 2007-08-10 2011-05-24 Genomatica, Inc. Methods and organisms for the growth-coupled production of 1,4-butanediol

Also Published As

Publication number Publication date
AU2008295788A1 (en) 2009-03-12
CN101883853A (zh) 2010-11-10
KR101042242B1 (ko) 2011-06-17
ES2396179T3 (es) 2013-02-19
CN101883853B (zh) 2013-12-25
AU2008295788B2 (en) 2013-01-17
ES2396179T5 (es) 2017-02-01
KR20090025902A (ko) 2009-03-11
EP2201115B1 (en) 2012-10-24
EP2201115A4 (en) 2010-09-22
US20150353964A1 (en) 2015-12-10
WO2009031766A2 (en) 2009-03-12
EP2201115B2 (en) 2016-07-20
WO2009031766A3 (en) 2009-04-30
DK2201115T3 (da) 2013-02-11
US9920325B2 (en) 2018-03-20
EP2201115A2 (en) 2010-06-30
US9096860B2 (en) 2015-08-04
US20100330634A1 (en) 2010-12-30

Similar Documents

Publication Publication Date Title
DK2201115T4 (da) Mutanter med evne til at producere 1,4-butandiol og fremgangsmåde til at fremstille 1,4-butandiol ved anvendelse heraf
Halmschlag et al. Tailor-made poly-γ-glutamic acid production
CN110079489B (zh) 一种重组盐单胞菌以及利用其生产P(3HB-co-4HB)的方法
KR20070096348A (ko) 1,4―ｂｕｔａｎｅｄｉｏｌ〔１，４―ＢＤＯ〕생성능을가지는 변이체 및 이를 이용한 １，４―ＢＤＯ의 제조방법
JP2012034702A (ja) 有機酸を高産生するための組換え微生物
CN100558886C (zh) 醛脱氢酶基因
EP1124979A4 (en) PRODUCTION OF 3-HYDROXYPROPIONIC ACID IN RECOMBINANT ORGANISMS
CN106062178B (zh) 具有增加的碳通量效率的方法和生物
CN114008197B (zh) 用于同时消耗木糖和葡萄糖以从第二代糖产生化学物质的代谢工程
HUE032951T2 (en) Microorganism for production of L-amino acid and method for producing L-amino acid using microorganism
KR20080031504A (ko) 악티노바실러스 숙시노제네스의 ｃ4-경로로부터 화학물질을생산하기 위해 악티노바실러스 숙시노제네스130ｚ(ａｔｃｃ 55618)로부터 얻은 유전자
WO2011163128A1 (en) Engineered bacteria produce succinate from sucrose
US12031123B2 (en) Recombinant bacterial cells and methods for producing poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
CN101679963A (zh) 用于产生3-羧基黏康酸内酯的基因被破坏的菌株、重组质粒、转化体和方法
US7588934B2 (en) Gene encoding fumarate hydratase C and use thereof
KR20140003258A (ko) 3-하이드록시프로피온산을 생산하는 재조합 미생물 및 이를 이용한 3-하이드록시프로피온산의 생산방법
KR102648170B1 (ko) 높은 글리세린 함량을 갖는 배양 배지 상에서의 발효에 의한 개선된 1,3-프로판디올 생산을 위한 미생물 및 방법
KR100957772B1 (ko) ４―ｈｙｄｒｏｘｙｂｕｔｙｒａｔｅ（４ＨＢ）생성능을가지는 변이체 및 이를 이용한 ４ＨＢ의 제조방법
KR101725368B1 (ko) (ｒ,ｒ)-2,3-부탄다이올 생산용 크렙시엘라 종 재조합 균주 및 (ｒ,ｒ)-2,3-부탄다이올 제조방법
CN116783281A (zh) 用于制备酰胺化合物的方法和组合物
KR102782737B1 (ko) 신규한 프로피온알데하이드 디하이드로게나제 활성을 갖는 변이체 폴리펩티드 및 이의 용도
KR102598992B1 (ko) 이소부탄올 및 폴리히드록시 부티레이트 동시 생산 균주 및 이의 이용
Edelshtein et al. Characterization of genes involved in poly-beta-hydroxybutyrate metabolism in Azospirillum brasilense
Liao Polymerase chain reaction based cloning of acetate kinase in propionibacterium acidipropionici