DK2201115T4 - Mutanter med evne til at producere 1,4-butandiol og fremgangsmåde til at fremstille 1,4-butandiol ved anvendelse heraf - Google Patents

Mutanter med evne til at producere 1,4-butandiol og fremgangsmåde til at fremstille 1,4-butandiol ved anvendelse heraf Download PDF

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DK2201115T4
DK2201115T4 DK08793214.1T DK08793214T DK2201115T4 DK 2201115 T4 DK2201115 T4 DK 2201115T4 DK 08793214 T DK08793214 T DK 08793214T DK 2201115 T4 DK2201115 T4 DK 2201115T4
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mutant
succinate
butanediol
gene
gene encoding
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DK2201115T3 (da
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Si Jae Park
Sang Hyun Lee
Sang Yup Lee
Eun Jeong Lee
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Lg Chemical Ltd
Korea Advanced Inst Sci & Tech
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    • C12Y103/99002—Butyryl-CoA dehydrogenase (1.3.99.2), i.e. short chain acyl-CoA dehydrogenase

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Claims (14)

1. Mutant, som udviser høj produktion af 1,4-butandiol, og som fremstilles ved at indføre eller amplificere gener, som koder for enzymer, der omdanner succi-nat til 4-hydroxybutyrat og 4-hydroxybutyrat til 1,4-butandiol, i en mikroorganisme i stand til at producere succinat, hvor mikroorganismen i stand til at producere succinat er en bakterie, og hvor genet, som koder for enzymet, som omdanner succinat til 4-hydroxybutyrat, er valgt fra gruppen bestående af gener, som koder for succinyl-CoA-trans-ferase, succinatsemialdehyddehydrogenase, og 4-hydroxyburatdehydrogenase, og genet, som koder for enzymet, der omdanner 4-hydroxybutyrat til 1,4-butandiol er et gen, der koder for phosphotransbutyrylase, et gen, som koder for butyryl-kinase og et gen, som koder for alkoholdehygrogenasereducerende 4-hydroxybutyrat-CoA.
2. Mutant ifølge krav 1, hvor bakterien er valgt fra gruppen bestående af vomflora, Corynebacterium sp., Brevibacterium sp. og E. coli.
3. Mutant ifølge krav 2, hvor vomflorabakterierne har inaktive gener, som koder for i) lactatdehydrogenase (IdhA) og pyruvatformatlyase (ptf), ii) lactatdrhy-drogenase {IhdA), pyruvatformatlyase {Pff), phosphotranacetylase (pta) og ace-tatkinase (ackA), eller iii) lactatdehydrogenase (IhdA), pyruvatformatlyase (Pfl) og phosphopyruvatcarboxylase (Ppc) og producerer succinat i en høj koncentration uden væsentlig produktion i anaerob tilstand af andre organiske syrer.
4. Mutant ifølge krav 2 eller 3, hvor vomflorabakterierne er valgt fra gruppen bestående af Mannheimia sp., Actinobacillus sp. og Anaerobiospirillum sp.
5. Mutant ifølge krav 4, hvor vomflorabakterierne er valgt fra gruppen bestående af Mannheimia succiniciproducens MBEL55E (KCTC 0769BP), og Marrn-heimia sp. LPK (KCTC 10558BP), LPK4 og LPK7 (KCTC 10626BP).
6. Mutant ifølge krav 2, hvor E. coli har inaktive gener, som koder for glucose-phosphotransferase (ptsG) og pyruvatkinase (pykA og pykF) og producerer succinat i høj koncentration uden væsentlig produktion af andre organiske syrer ved anaerobe betingelser.
7. Mutant ifølge krav 6, hvor E. coli-mutanten er W3110GFA.
8. Mutant ifølge krav 1, hvor genet, som koder for enzymet, der omdanner succinat til 4-hydroxybutyrat, stammer fra Clostridium kiuyveri.
9. Mutant ifølge krav 1, hvor genet, som koder for enzymet, der omdanner 4-hydroxybutyrat til 1,4-butandiol, stammer fra Clostridium acetobutylicum.
10. Mutant ifølge krav 1, hvor alkoholdehydrogenasen er butyl-CoA-dehydro-genase stammende fra Clostridium acetobutylicum.
11. Mutant ifølge krav 1, hvor mutanten har et inaktivt gen associeret med omdannelsen af succinatsemialdehyd til succinat.
12. Mutant ifølge krav 11, hvor genet associeret med omdannelse af succinatsemialdehyd til succinat er et gen, som koder for succinsemialdehyddehydro-genase.
13. Mutant ifølge krav 1, hvor et gen, som koder for C4-dicarboxy sent transportprotein (DctA) associeret med transport af succinat, yderligere indføres i eller amplificeres i mutanten.
14. Fremgangsmåde til at fremstille 1,4-butandiol omfattende: at dyrke mutanten ifølge et vilkårligt af kravene 1 til 13 i et medie indeholdende glucose, og opnå 1,4-butandiol fra mediet.
DK08793214.1T 2007-09-07 2008-08-13 Mutanter med evne til at producere 1,4-butandiol og fremgangsmåde til at fremstille 1,4-butandiol ved anvendelse heraf DK2201115T4 (da)

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Application Number Priority Date Filing Date Title
KR1020070091081A KR101042242B1 (ko) 2007-09-07 2007-09-07 1,4-부탄디올 생성능을 가지는 변이체 및 이를 이용한1,4-부탄디올의 제조방법
PCT/KR2008/004700 WO2009031766A2 (en) 2007-09-07 2008-08-13 Mutants having capability to produce 1,4-butanediol and method for preparing 1,4-butanediol using the same

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DK2201115T3 DK2201115T3 (da) 2013-02-11
DK2201115T4 true DK2201115T4 (da) 2016-11-07

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US (2) US9096860B2 (da)
EP (1) EP2201115B2 (da)
KR (1) KR101042242B1 (da)
CN (1) CN101883853B (da)
AU (1) AU2008295788B2 (da)
DK (1) DK2201115T4 (da)
ES (1) ES2396179T5 (da)
WO (1) WO2009031766A2 (da)

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AU2008295788B2 (en) 2013-01-17
WO2009031766A3 (en) 2009-04-30
KR20090025902A (ko) 2009-03-11
ES2396179T5 (es) 2017-02-01
DK2201115T3 (da) 2013-02-11
US9096860B2 (en) 2015-08-04
EP2201115B1 (en) 2012-10-24
US20150353964A1 (en) 2015-12-10
ES2396179T3 (es) 2013-02-19
CN101883853A (zh) 2010-11-10
EP2201115A2 (en) 2010-06-30
US20100330634A1 (en) 2010-12-30
WO2009031766A2 (en) 2009-03-12
EP2201115B2 (en) 2016-07-20
EP2201115A4 (en) 2010-09-22
AU2008295788A1 (en) 2009-03-12
US9920325B2 (en) 2018-03-20
KR101042242B1 (ko) 2011-06-17

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