DK2210946T3 - Mutant af proB-genet fra koryneforme bakterier - Google Patents
Mutant af proB-genet fra koryneforme bakterier Download PDFInfo
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- DK2210946T3 DK2210946T3 DK10157889.6T DK10157889T DK2210946T3 DK 2210946 T3 DK2210946 T3 DK 2210946T3 DK 10157889 T DK10157889 T DK 10157889T DK 2210946 T3 DK2210946 T3 DK 2210946T3
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- 102000006635 beta-lactamase Human genes 0.000 description 1
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- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
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- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
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- 235000013379 molasses Nutrition 0.000 description 1
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- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Claims (14)
1. Ikke-human værtsorganisme, der omfatter et polynukleotid, der koder for et polypeptid med en aminosyresekvens, der er mindst 90 % identisk med aminosyresekvensen ifølge SEQ ID NO: 2, hvor i aminosyresekvensen glycin i position 149 er erstattet af en proteinogenisk aminosyre, hvilket polypeptid har γ-glutamylkinaseaktivitet, hvor polynukleotidet overudtrykkes ved anvendelse af en stærk promotor, således at aktiviteten eller koncentrationen af det tilsvarende protein forøges med mindst 10 % i forhold til aktiviteten eller koncentrationen af proteinet i den oprindelige mikroorganisme.
2. Værtsorganisme ifølge krav 1, hvor aminosyreudskiftningen er udvalgt fra SEQ ID NO: 2, hvor glycin i position 149 er erstattet af en proteinogenisk aminosyre.
3. Værtsorganisme ifølge krav 1 eller 2, hvor glycinen i position 149 i SEQ ID NO: 2 er erstattet af L-asparaginsyre.
4. Værtsorganisme ifølge krav 3, hvor polynukleotidet omfatter nukleotidsekvensen ifølge SEQ ID NO: 3.
5. Ikke-human værtsorganisme, der omfatter et polynukleotid, der koder for et polypeptid, der fra position 145 til 154 har aminosyresekvensen fra position 145 til 154 i SEQ ID NO: 2, hvor polynukleotidet under stringente betingelser, der omfatter et vasketrin med 0,5 SSC ved 50-68 °C, hybridiserer til komplementet til SEQ ID NO: 3, hvilket polypeptid har γ-glutamylkinaseaktivitet.
6. Værtsorganisme ifølge krav 5, hvor det kodede polypeptid fra position 145 til 154 har aminosyresekvensen fra position 145 til 154 i SEQ ID NO: 4.
7. Værtsorganisme ifølge krav 5, hvor det kodede polypeptid fra position 110 til 189 har aminosyresekvensen fra position 110 til 189 i SEQ ID NO: 2.
8. Værtsorganisme ifølge krav 6, hvor det kodede polypeptid fra position 110 til 189 har aminosyresekvensen fra position 110 til 189 i SEQ ID NO: 4.
9. Værtsorganisme ifølge krav 1 til 8, hvor værtsorganismen er udvalgt fra gruppen, der omfatter gær, E. coli, B. subtilis, koryneforme bakterier.
10. Værtsorganisme ifølge krav 9, hvor værtsorganismen er en koryneform bakterie.
11. Værtsorganisme ifølge krav 10, hvor værtsorganismen er Corynebacterium glutamicum.
12. Fremgangsmåde til fremstilling af L-prolin, der omfatter a) fermentering af værtsorganismen ifølge et eller flere af kravene 1 til 12, b) berigelse af L-aminosyren i mediet eller i bakteriecellerne og c) isolering eller opsamling af aminosyren eventuelt med bestanddele fra fermenteringssuppen og/eller biomasse.
13. Fremgansgmåde ifølge krav 12, hvor værtsorganismer fermenteres, der omfatter et eller flere overudtrykte gener, der er udvalgt fra gruppen, der omfatter: a) gdh-genet, der koder for glutamatdehydrogenase, b) proA-genet, der koder for γ-glutamylphosphatreduktase, c) proC-genet, der koder for pyrolin-5-carboxylatreduktase og d) ocd-genet, der koder for ornithincyclodeaminase.
14. Fremgansgmåde ifølge krav 12, hvor en værtsorganisme fermenteres, der omfatter et eller flere svækkede gener, der er udvalgt fra gruppen, der omfatter: a) ilvA-genet, der koder for threonindeaminase, b) putA-genet, der koder for pralindehydrogenase/pyrolin-5-carboxylatdehydrogenase, c) sucA-genet, der koder for 2-ketoglutaratdehydrogenase, d) sucB-genet, der koder for dihydrolipoamidsuccinyltransferase, og e) argD-genet, der koder for acetylornithinaminotransferase.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102004061696A DE102004061696A1 (de) | 2004-12-22 | 2004-12-22 | Mutante des proB-Gens aus coryneformen Bakterien |
| EP05822785A EP1828384B1 (en) | 2004-12-22 | 2005-12-13 | Mutant of the prob gene from coryneform bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2210946T3 true DK2210946T3 (da) | 2015-06-08 |
Family
ID=36022639
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK05822785.1T DK1828384T3 (da) | 2004-12-22 | 2005-12-13 | Mutant af proB-genet fra coryneform-bakterier |
| DK10157889.6T DK2210946T3 (da) | 2004-12-22 | 2005-12-13 | Mutant af proB-genet fra koryneforme bakterier |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK05822785.1T DK1828384T3 (da) | 2004-12-22 | 2005-12-13 | Mutant af proB-genet fra coryneform-bakterier |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US7982020B2 (da) |
| EP (2) | EP2210946B1 (da) |
| JP (1) | JP4637183B2 (da) |
| KR (1) | KR100923806B1 (da) |
| CN (1) | CN101084312A (da) |
| AT (1) | ATE503016T1 (da) |
| BR (1) | BRPI0519207B1 (da) |
| DE (2) | DE102004061696A1 (da) |
| DK (2) | DK1828384T3 (da) |
| ES (2) | ES2537647T3 (da) |
| PL (1) | PL2210946T3 (da) |
| WO (1) | WO2006066758A1 (da) |
| ZA (1) | ZA200705143B (da) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103173466A (zh) * | 2012-06-20 | 2013-06-26 | 江南大学 | 敲除脯氨酸合成途径提高钝齿棒杆菌精氨酸产量的方法 |
| CN107603932B (zh) * | 2016-07-12 | 2020-07-21 | 中国科学院微生物研究所 | 一种提高谷氨酸棒杆菌氨基酸产量的方法及其应用 |
| EP3456833A1 (en) | 2017-09-18 | 2019-03-20 | Evonik Degussa GmbH | Method for the fermentative production of l-amino acids |
| EP3467099A1 (en) | 2017-10-05 | 2019-04-10 | Evonik Degussa GmbH | Method for the fermentative production of l-amino acids |
| CN109810989B (zh) * | 2017-11-21 | 2021-07-06 | 中国科学院微生物研究所 | Dna分子、表达载体、重组菌及其构建方法，以及羟脯氨酸的制备方法 |
| CN112111469B (zh) * | 2020-11-23 | 2021-02-26 | 中国科学院天津工业生物技术研究所 | γ-谷氨酰激酶突变体及其应用 |
| CN114763372B (zh) * | 2021-01-13 | 2024-12-10 | 中国科学院天津工业生物技术研究所 | 具有l-脯氨酸外排功能的蛋白及其应用 |
| WO2022161914A1 (en) | 2021-01-26 | 2022-08-04 | Basf Se | High temperature fermentation process and microorganisms |
| CN114107141B (zh) * | 2021-08-19 | 2022-07-12 | 中国科学院天津工业生物技术研究所 | 高产l-脯氨酸的谷氨酸棒杆菌以及高产l-脯氨酸的方法 |
| CN114181878B (zh) * | 2021-12-08 | 2025-04-08 | 上海交通大学 | 增强氨基酸合成基因的转录水平以提高tg酶发酵水平的方法 |
| KR102869970B1 (ko) * | 2023-06-23 | 2025-10-14 | 씨제이제일제당 (주) | 변이된 rbs 염기 서열을 포함하는 폴리뉴클리오티드, 상기 폴리뉴클레오티드를 포함하는 미생물, 및 이를 이용한 글리신 생산 방법 |
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| US7160711B2 (en) * | 2001-08-06 | 2007-01-09 | Degussa Ag | Coryneform bacteria which produce chemical compounds I |
-
2004
- 2004-12-22 DE DE102004061696A patent/DE102004061696A1/de not_active Withdrawn
-
2005
- 2005-12-13 JP JP2007547267A patent/JP4637183B2/ja not_active Expired - Lifetime
- 2005-12-13 BR BRPI0519207-2A patent/BRPI0519207B1/pt not_active IP Right Cessation
- 2005-12-13 ES ES10157889.6T patent/ES2537647T3/es not_active Expired - Lifetime
- 2005-12-13 US US11/722,301 patent/US7982020B2/en active Active
- 2005-12-13 DE DE602005027111T patent/DE602005027111D1/de not_active Expired - Lifetime
- 2005-12-13 PL PL10157889T patent/PL2210946T3/pl unknown
- 2005-12-13 EP EP10157889.6A patent/EP2210946B1/en not_active Expired - Lifetime
- 2005-12-13 EP EP05822785A patent/EP1828384B1/en not_active Expired - Lifetime
- 2005-12-13 ES ES05822785T patent/ES2362105T3/es not_active Expired - Lifetime
- 2005-12-13 DK DK05822785.1T patent/DK1828384T3/da active
- 2005-12-13 CN CNA2005800441304A patent/CN101084312A/zh active Pending
- 2005-12-13 KR KR1020077016519A patent/KR100923806B1/ko not_active Expired - Lifetime
- 2005-12-13 DK DK10157889.6T patent/DK2210946T3/da active
- 2005-12-13 AT AT05822785T patent/ATE503016T1/de active
- 2005-12-13 WO PCT/EP2005/013372 patent/WO2006066758A1/en not_active Ceased
-
2007
- 2007-06-21 ZA ZA200705143A patent/ZA200705143B/xx unknown
-
2011
- 2011-05-31 US US13/118,645 patent/US8343734B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI0519207B1 (pt) | 2021-03-30 |
| WO2006066758A1 (en) | 2006-06-29 |
| PL2210946T3 (pl) | 2015-08-31 |
| JP4637183B2 (ja) | 2011-02-23 |
| ATE503016T1 (de) | 2011-04-15 |
| CN101084312A (zh) | 2007-12-05 |
| EP2210946A1 (en) | 2010-07-28 |
| US7982020B2 (en) | 2011-07-19 |
| DK1828384T3 (da) | 2011-06-14 |
| ES2537647T3 (es) | 2015-06-10 |
| EP1828384A1 (en) | 2007-09-05 |
| EP1828384B1 (en) | 2011-03-23 |
| DE102004061696A1 (de) | 2006-07-06 |
| US8343734B2 (en) | 2013-01-01 |
| BRPI0519207A2 (pt) | 2008-12-30 |
| ES2362105T3 (es) | 2011-06-28 |
| JP2008523832A (ja) | 2008-07-10 |
| US20110318791A1 (en) | 2011-12-29 |
| EP2210946B1 (en) | 2015-03-11 |
| KR20070091663A (ko) | 2007-09-11 |
| ZA200705143B (en) | 2008-09-25 |
| US20090221043A1 (en) | 2009-09-03 |
| KR100923806B1 (ko) | 2009-10-27 |
| DE602005027111D1 (de) | 2011-05-05 |
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