ES2537647T3 - Mutante del gen proB de las bacterias corineformes - Google Patents

Mutante del gen proB de las bacterias corineformes Download PDF

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Publication number
ES2537647T3
ES2537647T3 ES10157889.6T ES10157889T ES2537647T3 ES 2537647 T3 ES2537647 T3 ES 2537647T3 ES 10157889 T ES10157889 T ES 10157889T ES 2537647 T3 ES2537647 T3 ES 2537647T3
Authority
ES
Spain
Prior art keywords
coryneform bacteria
gene mutant
prob gene
bacteria
prob
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES10157889.6T
Other languages
English (en)
Inventor
Stephan Hans
Brigitte Dr. Bathe
Georg Dr. Thierbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evonik Operations GmbH
Original Assignee
Evonik Degussa GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Degussa GmbH filed Critical Evonik Degussa GmbH
Application granted granted Critical
Publication of ES2537647T3 publication Critical patent/ES2537647T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

El organismo hospedante no humano que comprende un polinucleótido que codifica un polipéptido que tiene una secuencia de aminoácidos que es al menos un 90% idéntica a la secuencia de aminoácidos expuesta en SEC ID NO: 2, con lo que en las secuencias de aminoácidos la glicina en la posición 149 se sustituye por un aminoácido proteinogénico, con lo que el polipéptido tiene actividad de γ-glutamil cinasa, con lo que se sobreexpresa el polinucleótido utilizando un promotor potente de modo que la actividad o concentración de la proteína correspondiente se incremente en al menos un 10% basado en la actividad o concentración de la proteína en el microorganismo de partida.

Description

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Claims (1)

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ES10157889.6T 2004-12-22 2005-12-13 Mutante del gen proB de las bacterias corineformes Expired - Lifetime ES2537647T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004061696A DE102004061696A1 (de) 2004-12-22 2004-12-22 Mutante des proB-Gens aus coryneformen Bakterien
DE102004061696 2004-12-22

Publications (1)

Publication Number Publication Date
ES2537647T3 true ES2537647T3 (es) 2015-06-10

Family

ID=36022639

Family Applications (2)

Application Number Title Priority Date Filing Date
ES10157889.6T Expired - Lifetime ES2537647T3 (es) 2004-12-22 2005-12-13 Mutante del gen proB de las bacterias corineformes
ES05822785T Expired - Lifetime ES2362105T3 (es) 2004-12-22 2005-12-13 Mutante del gen prob de las bacterias corineformes.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES05822785T Expired - Lifetime ES2362105T3 (es) 2004-12-22 2005-12-13 Mutante del gen prob de las bacterias corineformes.

Country Status (13)

Country Link
US (2) US7982020B2 (es)
EP (2) EP2210946B1 (es)
JP (1) JP4637183B2 (es)
KR (1) KR100923806B1 (es)
CN (1) CN101084312A (es)
AT (1) ATE503016T1 (es)
BR (1) BRPI0519207B1 (es)
DE (2) DE102004061696A1 (es)
DK (2) DK1828384T3 (es)
ES (2) ES2537647T3 (es)
PL (1) PL2210946T3 (es)
WO (1) WO2006066758A1 (es)
ZA (1) ZA200705143B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103173466A (zh) * 2012-06-20 2013-06-26 江南大学 敲除脯氨酸合成途径提高钝齿棒杆菌精氨酸产量的方法
CN107603932B (zh) * 2016-07-12 2020-07-21 中国科学院微生物研究所 一种提高谷氨酸棒杆菌氨基酸产量的方法及其应用
EP3456833A1 (en) 2017-09-18 2019-03-20 Evonik Degussa GmbH Method for the fermentative production of l-amino acids
EP3467099A1 (en) 2017-10-05 2019-04-10 Evonik Degussa GmbH Method for the fermentative production of l-amino acids
CN109810989B (zh) * 2017-11-21 2021-07-06 中国科学院微生物研究所 Dna分子、表达载体、重组菌及其构建方法，以及羟脯氨酸的制备方法
CN112111469B (zh) * 2020-11-23 2021-02-26 中国科学院天津工业生物技术研究所 γ-谷氨酰激酶突变体及其应用
CN114763372B (zh) * 2021-01-13 2024-12-10 中国科学院天津工业生物技术研究所 具有l-脯氨酸外排功能的蛋白及其应用
WO2022161914A1 (en) 2021-01-26 2022-08-04 Basf Se High temperature fermentation process and microorganisms
CN114107141B (zh) * 2021-08-19 2022-07-12 中国科学院天津工业生物技术研究所 高产l-脯氨酸的谷氨酸棒杆菌以及高产l-脯氨酸的方法
CN114181878B (zh) * 2021-12-08 2025-04-08 上海交通大学 增强氨基酸合成基因的转录水平以提高tg酶发酵水平的方法
KR102869970B1 (ko) * 2023-06-23 2025-10-14 씨제이제일제당 (주) 변이된 rbs 염기 서열을 포함하는 폴리뉴클리오티드, 상기 폴리뉴클레오티드를 포함하는 미생물, 및 이를 이용한 글리신 생산 방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7160711B2 (en) * 2001-08-06 2007-01-09 Degussa Ag Coryneform bacteria which produce chemical compounds I

Also Published As

Publication number Publication date
BRPI0519207B1 (pt) 2021-03-30
WO2006066758A1 (en) 2006-06-29
PL2210946T3 (pl) 2015-08-31
JP4637183B2 (ja) 2011-02-23
ATE503016T1 (de) 2011-04-15
CN101084312A (zh) 2007-12-05
EP2210946A1 (en) 2010-07-28
US7982020B2 (en) 2011-07-19
DK2210946T3 (en) 2015-06-08
DK1828384T3 (da) 2011-06-14
EP1828384A1 (en) 2007-09-05
EP1828384B1 (en) 2011-03-23
DE102004061696A1 (de) 2006-07-06
US8343734B2 (en) 2013-01-01
BRPI0519207A2 (pt) 2008-12-30
ES2362105T3 (es) 2011-06-28
JP2008523832A (ja) 2008-07-10
US20110318791A1 (en) 2011-12-29
EP2210946B1 (en) 2015-03-11
KR20070091663A (ko) 2007-09-11
ZA200705143B (en) 2008-09-25
US20090221043A1 (en) 2009-09-03
KR100923806B1 (ko) 2009-10-27
DE602005027111D1 (de) 2011-05-05

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