DK2217729T3 - Fremgangsmåde til koncentrering af nukleinsyremolekyler - Google Patents

Fremgangsmåde til koncentrering af nukleinsyremolekyler Download PDF

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Publication number
DK2217729T3
DK2217729T3 DK08857258.1T DK08857258T DK2217729T3 DK 2217729 T3 DK2217729 T3 DK 2217729T3 DK 08857258 T DK08857258 T DK 08857258T DK 2217729 T3 DK2217729 T3 DK 2217729T3
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Denmark
Prior art keywords
nucleic acid
molecules
molecule
magnetic
magnetic beads
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DK08857258.1T
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English (en)
Inventor
Walter Gumbrecht
Peter Paulicka
Manfred Stanzel
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Boehringer Ingelheim Vetmed
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Publication of DK2217729T3 publication Critical patent/DK2217729T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Claims (2)

1
1. Fremgangsmåde til koncentrering af nukleinsyremolekyler (5a, 5b) på en overflade (201) af et sensorfelt (103a) af en biochip, hvor fremgangsmåden omfatter de følgende trin at: 5 (a) tilvejebringe magnetiske perler (1), med overførselsmolekyler (3a, 3b, 3c) im- mobiliseret derpå, som er i stand til at hybridisere til en del af nukleinsyremolekylerne (5a, 5b), som derved danner komplekser, som omfatter de magnetiske perler (1), overførselsmolekylerne (3a, 3b, 3c) og nukleinsyremolekylerne; tilvejebringe en overflade (201) af et sensorfelt (103a, 103b, 103c), som danner en 10 del af en overflade (101) af en biochip, hvor indfangningsmolekyler (203) er immobiliseret på overfladen (201), hvor indfangningsmolekylerne (203) specifikt hybridiserer til en del af nukleinsyremolekylerne (5a, 5b), hvor nukleinsyresekvenserne af indfangningsmolekylerne (203) og overførselsmolekylerne (3a, 3b, 3c) er valgt på en sådan måde at smeltetemperaturen af hybridet mellem et nukleinsyremolekyle (5a, 5b) og et overførselsmolekyle (3a, 15 3b, 3c) er mindre end smeltetemperaturen af hybridet mellem et nukleinsyremolekyle (5a, 5b) og et indfangningsmolekyle (203); og tilvejebringe en magnetisk feltgenerator (105); (b) inkubere de magnetiske perler (1) med nukleinsyremolekylerne (5a, 5b) ved en temperatur TI, som er under smeltetemperaturen af hybriderne mellem nukleinsyremole- 20 kylerne (5a, 5b) og overførselsmolekylerne (3a, 3b), som derved tillader nukleinsyremolekylerne (5a, 5b) at hybridisere til overførselsmolekylerne (3a, 3b); (c) at benytte et magnetisk felt gennem den magnetiske feltgenerator (105), som derved flytter nukleinsyremolekylerne (5a, 5b) bundet til de magnetiske perler (1) gennem det magnetisk felt til overfladen (101) af biochippen, som derved bringer nukleinsyremole- 25 kylerne (5a, 5b) bundet til de magnetiske perler (1) i kontakt med en overflade (201) af sensorfeltet (103a, 103b); (d) øge temperaturen fra en temperatur TI til en temperatur T2, som er over smeltetemperaturen af hybriderne af nukleinsyremolekylerne (5a, 5b) og overførselsmolekylerne (3a, 3b), og som er under smeltetemperaturen af hybriderne af nukleinsyremolekylerne 30 (5a, 5b) og indfangningsmolekylerne (203), som derved opløser nukleinsyremolekylerne (5a) fra de magnetiske perler (1) og tillade binding af mindst én del af nukleinsyremolekylerne (5a, 5b) til indfangningsmolekylet (203); (e) flytte de magnetiske perler (1) væk fra overfladen (101) af biochippen ved at afbryde det magnetiske field; 35 (f) gentage trinnene (b) til (e).
2. Fremgangsmåde ifølge krav 1, hvor forskellige specier af magnetiske perler (1) er tilvejebragt, hvor hver magnetisk perle, der har ét specifikt overførselsmolekyle (3a, 3b, 3c) immobiliseret derpå, som er i stand til at binde til et specifikt nukleinsyremolekyle (5a, 5b), som detekteres.
DK08857258.1T 2007-12-03 2008-11-03 Fremgangsmåde til koncentrering af nukleinsyremolekyler DK2217729T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07023377A EP2067867A1 (en) 2007-12-03 2007-12-03 Process for concentrating nucleic acid molecules
PCT/EP2008/064876 WO2009071404A1 (en) 2007-12-03 2008-11-03 Process for concentrating nucleic acid molecules

Publications (1)

Publication Number Publication Date
DK2217729T3 true DK2217729T3 (da) 2015-08-24

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DK08857258.1T DK2217729T3 (da) 2007-12-03 2008-11-03 Fremgangsmåde til koncentrering af nukleinsyremolekyler

Country Status (6)

Country Link
US (1) US8975017B2 (da)
EP (2) EP2067867A1 (da)
CN (1) CN101932730B (da)
DK (1) DK2217729T3 (da)
ES (1) ES2545584T3 (da)
WO (1) WO2009071404A1 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
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US8815610B2 (en) 2010-10-15 2014-08-26 International Business Machines Corporation Magnetic nanoparticle detection across a membrane
US10519487B2 (en) 2016-08-18 2019-12-31 City University Of Hong Kong Kit and a method for determining the presence or amount of a target nucleic acid sequence in a sample
WO2019157191A1 (en) * 2018-02-07 2019-08-15 Georgia Tech Research Corporation Methods for multiplexed cell isolation using dna gates
WO2025137866A1 (zh) * 2023-12-26 2025-07-03 深圳华大智造科技股份有限公司 一种用于上机测序的含核酸溶液的回收利用方法

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Also Published As

Publication number Publication date
EP2067867A1 (en) 2009-06-10
WO2009071404A1 (en) 2009-06-11
US8975017B2 (en) 2015-03-10
EP2217729A1 (en) 2010-08-18
CN101932730A (zh) 2010-12-29
EP2217729B1 (en) 2015-06-03
US20100248242A1 (en) 2010-09-30
CN101932730B (zh) 2014-03-26
ES2545584T3 (es) 2015-09-14

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