DK2256192T3 - Termostabil katalase - Google Patents

Termostabil katalase Download PDF

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Publication number
DK2256192T3
DK2256192T3 DK09711878.0T DK09711878T DK2256192T3 DK 2256192 T3 DK2256192 T3 DK 2256192T3 DK 09711878 T DK09711878 T DK 09711878T DK 2256192 T3 DK2256192 T3 DK 2256192T3
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DK
Denmark
Prior art keywords
ala
gly
ser
leu
val
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DK09711878.0T
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English (en)
Inventor
Kaoru Okakura
Fusuke Mazuka
Takayoshi Fukushima
Koichiro Murashima
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Meiji Seika Pharma Co Ltd
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Publication of DK2256192T3 publication Critical patent/DK2256192T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)
    • C12Y111/01006—Catalase (1.11.1.6)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (8)

1. Protein, der er udvalgt fra gruppen bestående af: (i) et protein, som omfatter aminosyresekvensen bestående af aminosyrer 1-692 af SEQ ID NO.:2; (ii) et protein, som omfatter en aminosyresekvens, hvor 1 til 50 aminosyrer er slettet, substitueret eller tilføjet aminosyresekvensen bestående af aminosyrer 1-692 af SEQ ID NO.: 2, og har en termostabil katalaseaktivitet; og (iii) et protein, som omfatter en aminosyresekvens, som har 80 % eller mere identitet med den, der består af aminosyrer 1-692 af SEQ ID NO.: 2, og har en termostabil katalaseaktivitet.
2. DNA, der er udvalgt fra gruppen bestående af: (i) et DNA, der koder for proteinet ifølge krav 1; og (ii) et DNA, som omfatter nukleotidsekvensen bestående af nukleotider 1-2403 af SEQ ID NO:1.
3. DNA ifølge krav 2, hvor en intronsekvens er fjernet fra DNA'et.
4. DNA ifølge krav 3, hvor intronsekvensen er en eller flere sekvenser, der er udvalgt fra nukleotidsekvensen bestående af nukleotider 322-372, 599-651, 1068-1113 eller 1279-1326 af SEQ ID NO: 1.
5. DNA ifølge et af kravene 2 til 4, hvor en nukleotidsekvens, der koder for en signalsekvens, er fjernet fra DNA'et.
6. DNA ifølge krav 5, hvor nukleotidsekvensen, der koder for en signalsekvens, er den, der består af nukleotider 1-126 af SEQ ID NO:1.
7. Ekspressionsvektor, som omfatter DNA'et ifølge et af kravene 2 til 6.
8. Værtmikroorganisme, som omfatter ekspressionsvektoren ifølge krav 7.
DK09711878.0T 2008-02-18 2009-02-18 Termostabil katalase DK2256192T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008036171 2008-02-18
PCT/JP2009/052729 WO2009104622A1 (ja) 2008-02-18 2009-02-18 耐熱性カタラーゼ

Publications (1)

Publication Number Publication Date
DK2256192T3 true DK2256192T3 (da) 2015-09-28

Family

ID=40985500

Family Applications (1)

Application Number Title Priority Date Filing Date
DK09711878.0T DK2256192T3 (da) 2008-02-18 2009-02-18 Termostabil katalase

Country Status (7)

Country Link
US (2) US8975053B2 (da)
EP (1) EP2256192B1 (da)
JP (1) JP5608372B2 (da)
CN (1) CN101970658B (da)
DK (1) DK2256192T3 (da)
ES (1) ES2547118T3 (da)
WO (1) WO2009104622A1 (da)

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BR112014014697A2 (pt) * 2011-12-19 2020-10-27 Novozymes, Inc. polipeptídeo isolado, composição, polinucleotídeo isolado, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, métodos para produzir um polipeptídeo e uma proteína, para gerar oxigênio molecular, e para remover peróxido de hidrogênio do tecido, processos para degradar ou converter um material celulósico, e para produzir um produto de fermentação, e, formulação de caldo integral ou composição de cultura de células
US9637725B2 (en) 2011-12-19 2017-05-02 Novozymes Inc. Polypeptides having catalase activity and polynucleotides encoding same
CN102559714A (zh) * 2012-01-31 2012-07-11 福州大学 一种编码过氧化氢酶的基因及其制备方法和应用
EP2861715B1 (en) * 2012-06-13 2018-09-19 Eucodis Bioscience GmbH Catalase in growth media
DK2959004T3 (da) 2013-02-21 2021-03-01 Novozymes As Fremgangsmåder til forsukring og fermentering af et celluloseholdigt materiale
US20160298154A1 (en) 2013-09-04 2016-10-13 Novozymes A/S Processes for Increasing Enzymatic Hydrolysis of Cellulosic Material
DK3063285T3 (da) 2013-11-01 2019-05-13 Novozymes As Fremgangsmåder til forsukring og fermentering af et celluloseholdigt materiale
EP3191597A1 (en) 2014-08-21 2017-07-19 Novozymes A/S Process for saccharifying cellulosic material under oxygen addition
CN104212820B (zh) * 2014-09-15 2016-09-21 青岛蔚蓝生物集团有限公司 一种具有过氧化氢酶活性的酶及其编码基因
WO2016045569A1 (en) 2014-09-23 2016-03-31 Novozymes A/S Processes for producing ethanol and fermenting organisms
EP3344761A1 (en) 2015-09-04 2018-07-11 Novozymes A/S Methods of inhibiting aa9 lytic polysaccharide monooxygenase catalyzed inactivation of enzyme compositions
CN107384886B (zh) * 2017-08-28 2020-10-13 王艺璇 一种过氧化氢酶及其应用
CN110540974B (zh) * 2019-09-29 2020-07-03 上海市农业科学院 草菇过氧化氢酶vcat及其编码基因和应用
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN118652862A (zh) * 2024-06-28 2024-09-17 青岛蔚蓝生物集团有限公司 过氧化氢酶突变体

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Also Published As

Publication number Publication date
HK1149048A1 (en) 2011-09-23
US8975053B2 (en) 2015-03-10
CN101970658A (zh) 2011-02-09
WO2009104622A1 (ja) 2009-08-27
US20150132820A1 (en) 2015-05-14
CN101970658B (zh) 2014-02-26
EP2256192B1 (en) 2015-07-15
ES2547118T3 (es) 2015-10-01
US9512409B2 (en) 2016-12-06
JPWO2009104622A1 (ja) 2011-06-23
EP2256192A1 (en) 2010-12-01
US20100330646A1 (en) 2010-12-30
EP2256192A4 (en) 2012-06-06
JP5608372B2 (ja) 2014-10-15

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