DK2287300T3 - Bakterietoksinvaccine - Google Patents
Bakterietoksinvaccine Download PDFInfo
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- DK2287300T3 DK2287300T3 DK09738819.3T DK09738819T DK2287300T3 DK 2287300 T3 DK2287300 T3 DK 2287300T3 DK 09738819 T DK09738819 T DK 09738819T DK 2287300 T3 DK2287300 T3 DK 2287300T3
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- ser
- dna
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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- C—CHEMISTRY; METALLURGY
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/25—Shigella (G)
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
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- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Claims (16)
1. Hybridprotein omfattende (a) to toksinproteiner valgt fra gruppen bestående af Shigatoksin (Stx)-protein, choleratoksin (CT)-protein- eller Escherichia coli varme-labilt toksin (LT)-protein, hvor toksinproteinerne er serieforbundet via et peptid med aminosyresekvensen repræsenteret ved SEQ ID NO: 2, 82 eller 84, og (b) et peptid med aminosyresekvensen repræsenteret ved SEQ ID NO: 2, 82 eller 84 forbundet med C-terminus på de serieforbundne toksinproteiner ifølge (a), hvor Stx-proteinet er et STx2eB-protein, hvor CT-proteinet er en CT-protein B subenhed og hvor LT proteinet er en LT protein B subenhed.
2. Hybridprotein ifølge krav 1, hvor toksinproteinerne er serieforbundet via et peptid med aminosyresekvensen repræsenteret ved SEQ ID NO:2.
3. Hybridprotein ifølge krav 1 eller 2, omfattende en aminosyresekvens repræsenteret ved SEQ ID NO: 14 eller 16.
4. Hybridprotein ifølge krav 1, omfattende en aminosyresekvens repræsenteret ved SEQ ID NO: 90, 92, 98 eller 100.
5. Hybridprotein ifølge ethvert af kravene 1 til 4, hvor et sekretorisk signalpeptid afledt fra en plante er tilføjet til dettes aminoterminus.
6. Hybridprotein ifølge ethvert af kravene 1 til 5, hvor et endoplasmisk reticulum-tilbageholdelsessignalpeptid er tilføjet til dettes carboxylterminus.
7. Et DNA-konstrukt, som omfatter DNA, som koder for hybridproteinet ifølge ethvert af kravene 1 til 6.
8. DNA-konstrukt ifølge krav 7, omfattende DNA med en basissekvens repræsenteret ved SEQ ID NO: 13, 15, 89,91,97 eller 99.
9. DNA-konstrukt ifølge krav 7 eller 8, hvor DNA'et, som koder for et hybridprotein, er funktionsmæssigt forbundet med en 5'-ikke-translateret region af et alkoholdehydrogenasegen afledt fra en plante.
10. DNA-konstrukt ifølge krav 9, hvor den 5'-ikke-translaterede region af et alkoholdehydrogenasegen afledt fra en plante er afledt fra Nicotiana tabacum.
11. DNA-konstrukt ifølge krav 10, omfattende en basissekvens repræsenteret ved enhver af SEQ ID NOS: 28, 29, 101, 102, 103, 104, 108, 109, 110 eller 111.
12. Rekombinant vektor omfattende DNA-konstruktet ifølge ethvert af kravene 7 til 11.
13. Værtscelle transformeret med den rekombinante vektor ifølge krav 12.
14. Værtscelle ifølge krav 13, hvor transformanten er en transformeret plantecelle eller en transformeret plante.
15. Frø, som er opnået fra transformanten ifølge krav 13 eller 14.
16. Peptid med en aminosyresekvens repræsenteret ved én af SEQ ID NO: 2, 82 eller 84.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008120573 | 2008-05-02 | ||
| PCT/JP2009/058345 WO2009133882A1 (ja) | 2008-05-02 | 2009-04-28 | 細菌毒素ワクチン |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2287300T3 true DK2287300T3 (da) | 2018-09-17 |
Family
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| DK09738819.3T DK2287300T3 (da) | 2008-05-02 | 2009-04-28 | Bakterietoksinvaccine |
| DK14152631.9T DK2728004T3 (da) | 2008-05-02 | 2009-04-28 | Bakterietoksinvaccine |
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| DK14152631.9T DK2728004T3 (da) | 2008-05-02 | 2009-04-28 | Bakterietoksinvaccine |
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| US (2) | US8846052B2 (da) |
| EP (2) | EP2287300B1 (da) |
| JP (1) | JP5360727B2 (da) |
| KR (1) | KR101540496B1 (da) |
| CN (2) | CN102016034B (da) |
| AR (1) | AR071232A1 (da) |
| DK (2) | DK2287300T3 (da) |
| ES (2) | ES2691985T3 (da) |
| MX (1) | MX2010011938A (da) |
| TW (1) | TWI460270B (da) |
| WO (1) | WO2009133882A1 (da) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102016034B (zh) * | 2008-05-02 | 2014-11-05 | 出光兴产株式会社 | 细菌毒素疫苗 |
| KR20130079362A (ko) | 2010-03-25 | 2013-07-10 | 내셔날 유니버시티 코포레이션 유니버시티 오브 토야마 | 형질 세포 동정 및 단리용 형광 프로브 및 이 프로브를 이용한 형질 세포의 동정 또는 단리방법 |
| JP5641205B2 (ja) * | 2010-07-13 | 2014-12-17 | 出光興産株式会社 | 糖鎖修飾が抑制された改変Ｓｔｘ２ｅタンパク質 |
| JP5963746B2 (ja) * | 2011-03-30 | 2016-08-03 | 国立大学法人富山大学 | 形質細胞または形質芽細胞の選択方法、目的抗原特異的な抗体の製造方法、新規モノクローナル抗体 |
| MX368398B (es) * | 2012-10-22 | 2019-10-01 | Jectas Innovators Company Ltd | Vacuna para prevenir la enfermedad de edema porcino. |
| US9937271B2 (en) | 2013-11-26 | 2018-04-10 | Idemitsu Kosan Co., Ltd. | Prevention of Escherichia coli diarrhea |
| US9981027B2 (en) | 2013-11-26 | 2018-05-29 | Idemitsu Kosan Co., Ltd. | Vaccine against colibacillosis |
| WO2016021276A1 (ja) * | 2014-08-08 | 2016-02-11 | 出光興産株式会社 | 豚繁殖・呼吸障害症候群防除剤 |
| CN108290961A (zh) | 2015-11-30 | 2018-07-17 | 出光兴产株式会社 | 免疫原性得到增强的疫苗抗原 |
| TW201726706A (zh) * | 2015-12-28 | 2017-08-01 | 出光興產股份有限公司 | 胜肽標記及含有其之附加標記的蛋白質 |
| JPWO2017217460A1 (ja) * | 2016-06-15 | 2019-04-04 | 出光興産株式会社 | ペプチドリンカーで連結された２以上のタンパク質を含む融合タンパク質 |
| JPWO2018225662A1 (ja) * | 2017-06-07 | 2020-04-09 | 出光興産株式会社 | 自然免疫増強剤 |
| WO2020045530A1 (ja) * | 2018-08-31 | 2020-03-05 | 出光興産株式会社 | ペプチドタグを利用したタンパク質の可溶性発現 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1339307C (en) | 1988-09-06 | 1997-08-19 | Roy Curtiss, Iii | Oral immunization by transgenic plants |
| US5525491A (en) * | 1991-02-27 | 1996-06-11 | Creative Biomolecules, Inc. | Serine-rich peptide linkers |
| ATE174628T1 (de) * | 1992-07-31 | 1999-01-15 | Medeva Holdings Bv | Expression rekombinanter fusionsproteine in attenuierten bakterien |
| MX9703017A (es) | 1994-10-24 | 1997-10-31 | Texas A & M Univ Sys | Inmunizacion oral con plantas transgenicas. |
| CA2252439C (en) | 1996-04-19 | 2014-09-30 | Henry M. Jackson Foundation For The Advancement Of Military Medicine | Method of stimulating an immune response by administration of host organisms that express intimin alone or as a fusion protein with one or more other antigens |
| WO2000037609A2 (en) | 1998-12-22 | 2000-06-29 | Boyce Thompson Institute For Plant Research At Cornell | Orally immunogenic bacterial enterotoxins expressed in transgenic plants |
| AU2001271268A1 (en) * | 2000-05-19 | 2001-12-03 | The Administrators Of The Tulane Educational Fund | Hybrid lt-a/ct-b holotoxin for use as an adjuvant |
| JP4627879B2 (ja) | 2000-12-28 | 2011-02-09 | 国立大学法人 奈良先端科学技術大学院大学 | 植物細胞において遺伝子の高発現能を有する５’−非翻訳領域配列 |
| US7807184B2 (en) * | 2003-07-21 | 2010-10-05 | Interuet International B.V. | Hybrid Shiga-like toxin |
| CN101016332B (zh) * | 2007-03-07 | 2010-12-01 | 中国人民解放军军事医学科学院微生物流行病研究所 | 一种抑制志贺毒素的短肽及其用途 |
| EP2169054B1 (en) * | 2007-07-03 | 2015-12-23 | Idemitsu Kosan Co., Ltd. | Pig edema disease vaccine |
| CN102016034B (zh) * | 2008-05-02 | 2014-11-05 | 出光兴产株式会社 | 细菌毒素疫苗 |
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- 2009-04-28 CN CN200980116606.9A patent/CN102016034B/zh not_active Expired - Fee Related
- 2009-04-28 EP EP09738819.3A patent/EP2287300B1/en active Active
- 2009-04-28 DK DK09738819.3T patent/DK2287300T3/da active
- 2009-04-28 DK DK14152631.9T patent/DK2728004T3/da active
- 2009-04-28 ES ES14152631.9T patent/ES2691985T3/es active Active
- 2009-04-28 CN CN201310566988.3A patent/CN103626876B/zh not_active Expired - Fee Related
- 2009-04-28 US US12/990,597 patent/US8846052B2/en active Active
- 2009-04-28 EP EP14152631.9A patent/EP2728004B1/en active Active
- 2009-04-28 JP JP2010510133A patent/JP5360727B2/ja not_active Expired - Fee Related
- 2009-04-28 ES ES09738819.3T patent/ES2686780T3/es active Active
- 2009-04-28 MX MX2010011938A patent/MX2010011938A/es active IP Right Grant
- 2009-04-28 WO PCT/JP2009/058345 patent/WO2009133882A1/ja not_active Ceased
- 2009-04-28 KR KR1020107026745A patent/KR101540496B1/ko active Active
- 2009-04-29 AR ARP090101546A patent/AR071232A1/es unknown
- 2009-04-30 TW TW098114432A patent/TWI460270B/zh not_active IP Right Cessation
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Also Published As
| Publication number | Publication date |
|---|---|
| US8846052B2 (en) | 2014-09-30 |
| EP2728004B1 (en) | 2018-08-08 |
| MX2010011938A (es) | 2010-12-01 |
| JPWO2009133882A1 (ja) | 2011-09-01 |
| KR20110009197A (ko) | 2011-01-27 |
| EP2287300A4 (en) | 2011-06-22 |
| US20110231960A1 (en) | 2011-09-22 |
| CN102016034A (zh) | 2011-04-13 |
| DK2728004T3 (da) | 2018-11-05 |
| AR071232A1 (es) | 2010-06-02 |
| EP2287300A1 (en) | 2011-02-23 |
| CN103626876A (zh) | 2014-03-12 |
| US20150133635A1 (en) | 2015-05-14 |
| KR101540496B1 (ko) | 2015-08-25 |
| WO2009133882A1 (ja) | 2009-11-05 |
| ES2691985T3 (es) | 2018-11-29 |
| EP2728004A3 (en) | 2014-07-23 |
| ES2686780T3 (es) | 2018-10-19 |
| CN103626876B (zh) | 2016-09-14 |
| TW201005093A (en) | 2010-02-01 |
| CN102016034B (zh) | 2014-11-05 |
| EP2728004A2 (en) | 2014-05-07 |
| JP5360727B2 (ja) | 2013-12-04 |
| TWI460270B (zh) | 2014-11-11 |
| EP2287300B1 (en) | 2018-06-27 |
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