DK2330181T3 - Automatiseret vævsmanipulationssystem - Google Patents

Automatiseret vævsmanipulationssystem Download PDF

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DK2330181T3
DK2330181T3 DK10186252.2T DK10186252T DK2330181T3 DK 2330181 T3 DK2330181 T3 DK 2330181T3 DK 10186252 T DK10186252 T DK 10186252T DK 2330181 T3 DK2330181 T3 DK 2330181T3
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bioreactor
tissue
cell
cells
proliferation
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Timothy J N Smith
Sydney M Pugh
Martin R Pecaric
Rupert Hagg
Roberto Tommasini
Yves Larcher
D Lowell Misener
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Octane Biotech Inc
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Claims (16)

  1. AUTOMATISERET VÆVSMANIPULATIONSSYSTEM
    1. Fremgangsmåde til (a) automatiseret proliferation af autologe, allogene eller xenogene celler til anvendelse i vævsmanipulationsfremgangsmåder, hvilken fremgangsmåde omfatter; - podning af celler på eller inde i et proliferationssubstrat eller -skelet, der understøttes inde i en bioreaktor (202), som er forbundet med et mediereservoir og strømningssystem (206), hvilken bioreaktor (202) har sensorer (132,134) til detektion af ændringer i miljøbetingelser, hvilket omfatter pH-værdi og temperatur samt opløste gasser inde i bioreaktoren (202), hvilke sensorer (132,134) genererer signaler, der overvåges af en mikroprocessor (128), og hvilken mikroprocessor (128) styrer og tilpasser det indre miljø i bioreaktoren (202) med henblik på at opfylde kravene til celleproliferation inde i bioreaktoren (202); - overvågning og opretholdelse af egnede dyrkningsbetingelser inde i bioreaktoren (202) i et tilstrækkeligt tidsrum til et ønsket niveau af celleproliferation, hvor statussen for celleproliferation bedømmes indirekte ved detektion af metabolisk omsætning som en funktion af tid, og bedømmelsen af statussen for celleproliferation omfatter bestemmelse af konfluensniveauet ved sensorbaseret overvågning.
  2. 2. Fremgangsmåde ifølge krav 1, der endvidere omfatter (b) automatiseret differentiering af celler, hvilken fremgangsmåde omfatter: - podning af celler på eller inde i et differentieringssubstrat eller -skelet, der understøttes inde i en bioreaktor (202), som er forbundet med et mediereservoir og strømningssystem (206), hvilken bioreaktor (202) har sensorer (132,134) til detektion af ændringer i omgivelsesbetingelser, hvilket omfatter pH-værdi og temperatur samt opløste gasser inde i bioreaktoren (202), hvilke sensorer (132,134) genererer signaler, der overvåges af mikroprocessoren (128), og hvilken mikroprocessor (128) overvåger progressionen af celledifferentiering og tilpasser miljøbetingelserne i bioreaktoren (202) med henblik på at opfylde kravene til celledifferentiering inde i bioreaktoren (202); - overvågning af fremskridtet for celledifferentiering inde i bioreaktoren (202) med henblik på at opretholde egnede dyrkningsbetingelser inde i bioreaktoren (202) i et tilstrækkeligt tidsrum til et ønsket niveau af celledifferentiering.
  3. 3. Fremgangsmåde ifølge krav 1 eller 2, der endvidere omfatter (c) fremstilling af en vævskonstruktion, hvilken fremgangsmåde omfatter: - podning af celler på eller inde i et skelet, hvilket skelet understøttes i en bioreaktor (202), som er forbundet med et mediereservoir og strømningssystem (206), hvilken bioreaktor (202) har sensorer (132,134) til detektion af ændringer i miljøbetingelser, hvilket omfatter pH og temperatur samt opløste gasser inde i bioreaktoren (202), hvilke sensorer (132,134) genererer signaler, der overvåges af en mikroprocessor (128), og hvilken mikroprocessor (128) overvåger progressionen af vævsudvikling og tilpasser miljøbetingelserne i bioreaktoren (202) med henblik på at opfylde kravene til vævsdannelse inde i bioreaktoren (202); - overvågning af fremskridtet for vævsudvikling med henblik på at opretholde egnede dyrkningsbetingelser inde i bioreaktoren (202) i et tilstrækkeligt tidsrum til, at cellerne kan udtrykke ekstracellulær matrix, der tilvejebringer strukturel understøtning for vævskonstruktionen.
  4. 4. Fremgangsmåde ifølge krav 1,2 eller 3, hvor målinger af pH-værdi, O2, CCteog glucoseforbrug anvendes til at detektere metabolisk omsætning.
  5. 5. Fremgangsmåde ifølge krav 1, hvor den sensorbaserede overvågning omfatter optisk overvågning via CCD-kamera.
  6. 6. Fremgangsmåde ifølge et hvilket som helst af kravene 3 til 5, hvor den dannede vævskonstruktion er et bruskimplantat, skeletimplantat eller en indre kerne i en rygmarvsskive.
  7. 7. Automatiseret cellekultur og/eller vævsmanipulationssystem, der omfatter; - én eller flere bioreaktorer (202), idet hver af bioreaktorerne (202) omfatter ét eller flere kamre deri, som er egnet til i brug at fremme fysiologiske cellefunktioner og/eller generering af én eller flere cellepopulationer og/eller vævskonstruktioner på en sekventiel og/eller samtidig processeringsmåde inde i og/eller mellem ønskede kamre og/eller bioreaktorer (202); hvor den ene eller flere bioreaktorer (202) er operativt forbundet med mediereservoir og strømningssystem (206), - sensorer (132,134), der er associeret med den ene eller flere bioreaktorer (202), til detektering af ændringer i miljøbetingelser, hvilket omfatter pH-værdi og temperatur samt opløste gasser inde i bioreaktoren (202), og hvilke sensorer (132,134) genererer signaler, der overvåges afen mikroprocessor (128); - middel til detektion af metabolisk omsætning som en funktion af tid til bedømmelse af statussen for celleproliferation; - sensorbaseret overvågningsmiddel til bestemmelse af konfluensniveauet; - en mikroprocessor (128) til fremkaldelse af den sekventielle og/eller samtidige biologiske processering i brug, og som i brug styrer og tilpasser det indre miljø i bioreaktoren (202) med henblik på at opfylde kravene til de forskellige cellekultur-og/eller vævsudviklingsstadier inde i bioreaktoren (202) som reaktion på overvågning af de signaler, der genereres af sensorerne (132,134); hvor mikroprocessoren (128) i brug overvåger progressionen af autolog, allogen eller xenogen celleproliferation og valgfrit celledifferentiering og/eller vævsudvikling og opretholder egnede dyrkningsbetingelser inde i bioreaktoren (202) i et tilstrækkeligt tidsrum til et ønsket niveau af celleproliferation og valgfrit i et tilstrækkeligt tidsrum til et ønsket niveau af celledifferentiering og/eller i et tilstrækkeligt tidsrum til, at cellerne kan udtrykke ekstracellulær matrix, der tilvejebringer strukturel understøtning for vævskonstruktionen.
  8. 8. System ifølge krav 7, hvor detektion af metabolisk omsætning som en funktion af tid omfatter måling af pH-værdi, O2, CO2 og glucoseforbrug.
  9. 9. System ifølge krav 7, hvor det sensorbaserede overvågningsmiddel omfatter et optisk overvågningsmiddel, hvor det optiske overvågningsmiddel omfatter et CCD-kamera.
  10. 10. System ifølge et hvilket som helst af kravene 7 til 9, hvor det ene eller flere kamre er valgt fra gruppen bestående af et vævsspaltningskammer, kultur- og proliferationskammer, differentierings- og vævsdannelseskammer samt kombinationer deraf.
  11. 11. System ifølge et hvilket som helst af kravene 7 til 10, hvor kamrene og/eller bioreaktorerne (202) tilvejebringes operabelt forbundet.
  12. 12. System ifølge et hvilket som helst af kravene 7 til 11, hvormindst ét af kamrene og/eller bioreaktorerne (202) er én af mindst uafhængigt operabelt og kooperativt operabelt.
  13. 13. System ifølge et hvilket som helst af kravene 7 til 12, hvormindst ét af kamrene og bioreaktorerne (202) er operabelt forbundet med henblik på i brug at tilvejebringe udskiftning af én eller flere af fluider, celler og væv.
  14. 14. System ifølge et hvilket som helst af kravene 7 til 13, hvor de to eller flere kamre og/eller bioreaktorer (202) er parallelt og/eller seriemæssigt operable.
  15. 15. System ifølge et hvilket som helst af kravene 7 til 14, hvor systemet endvidere omfatter én eller flere sensorer (132,134), der er egnet til overvågning i brug af parametre i forbindelse med de fysiologiske cellefunktioner og/eller generering af cellepopulationer og/eller vævskonstruktioner på den sekventielle og/eller samtidige processeringsmåde.
  16. 16. System ifølge et hvilket som helst af kravene 7 til 15, hvor systemet i brug udfører og/eller tilvejebringer én eller flere af følgende: -steril modtagelse/opbevaring af vævsbiopsi; - blanding og afgivelse af spaltningsreagenser; - overvågning af en spaltningsproces; - spaltning af biopsivæv til dannelse af dissocierede celler; - cellesortering og -valg; - cellepodning på eller inde i et proliferationssubstrat eller -skelet; - blanding og afgivelse af proliferationsreagenser; - proliferation af celler til ekspansion af cellepopulationer; - overvågning af cellebetingelser; - detektion af konfluens; - "controlled release" fra et proliferationssubstrat eller -skelet; - cellevaskning og celleindsamling; - cellepodning på eller inde i et vævsmanipulationsskelet eller en matrix; - blanding og afgivelse af differentieringsreagenser; - overvågning af celle- og vævskulturbetingelser; - celledifferentiering for at muliggøre specialisering af celleaktivitet; - vævsdannelse; - mekanisk og/eller biokemisk stimulering til fremme af vævsmodenhed; - affaldsindsamling; - høstning af vævsmanipulerede konstruktioner/implantater; og - opbevaring og transport af celler og/eller væv.
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Families Citing this family (165)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230357696A1 (en) * 2001-05-28 2023-11-09 University Of Virginia Patent Foundation Hermetically or aseptically sealed bioreactor system and related method thereof
WO2005116186A1 (en) * 2004-05-26 2005-12-08 Millenium Biologix Inc. Advanced tissue engineering system
AU2005287162B2 (en) * 2004-09-16 2010-12-23 Becton, Dickinson And Company Perfusion bioreactor for culturing cells
US7767446B2 (en) * 2004-09-16 2010-08-03 Becton, Dickinson And Company Perfusion bioreactors for culturing cells
WO2006060214A2 (en) 2004-11-18 2006-06-08 The Regents Of The University Of California Apparatus and methods for manipulation and optimization of biological systems
US20080274545A1 (en) * 2005-03-02 2008-11-06 Government Of The Us, As Represented By The Secret Bioreactor Chamber Apparatus, and Method and System for Fabricating and Mechanically Stimulating Natural and Engineered Tissues
GB0505379D0 (en) * 2005-03-16 2005-04-20 Robio Systems Ltd Cellular entity maturation and transportation systems
WO2007012071A1 (en) * 2005-07-20 2007-01-25 Government Of The United States Of America, As Represented By The Secretary, Department Of Health Bioreactor device, and method and system for fabricating tissues in the bioreactor device
EP1924680A2 (en) * 2005-09-16 2008-05-28 Becton, Dickinson & Company Scaffold carrier cartridge
JP5121208B2 (ja) * 2005-11-07 2013-01-16 キヤノン株式会社 生体組織処理用の基板、処理装置、処理方法及び処理キット
WO2007139748A2 (en) 2006-05-22 2007-12-06 Biovest International Inc. Extra-capillary fluid cycling system and method for a cell culture device
EP2049159A4 (en) * 2006-08-11 2011-08-17 Univ Queensland SCAFFOLD TREATMENT DEVICE AND METHOD
US8275594B2 (en) * 2006-10-30 2012-09-25 The Regents Of The University Of Michigan Engineered scaffolds for intervertebral disc repair and regeneration and for articulating joint repair and regeneration
KR100824357B1 (ko) 2006-12-26 2008-04-23 주식회사 바이오트론 소형 생물반응기
CN101622337B (zh) * 2007-03-01 2013-04-24 泰尔茂比司特公司 用于与细胞扩增装置一起使用的一次性管道成套设备和无菌取样的方法
US8795194B2 (en) 2007-03-30 2014-08-05 Smith & Nephew, Inc. Tissue harvesting
US20080294361A1 (en) * 2007-05-24 2008-11-27 Popp Shane M Intelligent execution system for the monitoring and execution of vaccine manufacturing
KR20100059789A (ko) * 2007-06-29 2010-06-04 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 배아줄기세포 배양을 위한 자동화 방법 및 장치
EP2187985B1 (en) * 2007-08-14 2016-09-28 Smith & Nephew PLC Scaffolds
EP2203046B1 (en) * 2007-09-28 2015-05-27 Organ Assist B.V. Organ transport container system
WO2009073724A1 (en) 2007-12-04 2009-06-11 Ingeneron, Inc. Apparatus and methods for cell isolation
US8865199B2 (en) * 2008-11-17 2014-10-21 Ingeneron, Inc. Biomatrix composition and methods of biomatrix seeding
US9687348B2 (en) * 2009-03-03 2017-06-27 The Trustees Of Columbia University In The City Of New York Method of making a personalized bone graft
DE102009022351A1 (de) * 2009-05-15 2010-11-25 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Modulares Produktionssystem zur automatischen Herstellung von dreidimensionalen Gewebestrukturen
DE102009022354B4 (de) * 2009-05-15 2015-05-07 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Bioreaktorsystem
ES2372108B1 (es) * 2009-10-26 2013-02-11 Ebers Medical Technology, S.L Biorreactor de flujo para cultivo celular.
US9206383B2 (en) * 2009-12-07 2015-12-08 The Trustees Of Columbia University In The City Of New York Bioreactor, devices, systems and methods
GB201004614D0 (en) 2010-03-19 2010-05-05 Ge Healthcare Uk Ltd A system and method for automated extraction of multi-cellular physiological parameters
WO2011130865A2 (en) 2010-04-21 2011-10-27 Yves Larcher Automated cell culture system
BR112012026882A2 (pt) * 2010-05-17 2015-09-22 Pall Corp sistemas para semear células em sustentações tri-dimensionais
US9090863B2 (en) 2010-05-17 2015-07-28 Pall Corporation System for seeding cells onto three dimensional scaffolds
US9115336B2 (en) 2010-05-25 2015-08-25 Muffin Incorporated Methods, substrates, and systems useful for cell seeding of medical grafts
KR20130123366A (ko) * 2010-07-01 2013-11-12 가부시키가이샤 가네카 세포 배양용 디스포저블 세트, 세포 배양 장치 및 세포 조제 방법
WO2012048275A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
JP2012090584A (ja) * 2010-10-27 2012-05-17 Inoac Gijutsu Kenkyusho:Kk 反重力培養方法及び反重力培養装置
EP2500410A1 (en) * 2011-03-18 2012-09-19 Ludwig-Maximilians-Universität München Bioreactor with mechanical and electrical stimulation means
EP3557251B1 (en) * 2011-03-22 2023-09-13 CartiRegen B.V. A cartilage cell processing system
US20140105938A1 (en) * 2011-03-22 2014-04-17 Cellcotec B.V. Cartilage cell processing system
WO2012171030A2 (en) 2011-06-10 2012-12-13 Biovest International, Inc. Method and apparatus for antibody production and purification
US20140322805A1 (en) * 2011-07-25 2014-10-30 General Atomics System and Method for Using a Pulse Flow Circulation for Algae Cultivation
KR101880676B1 (ko) * 2011-08-22 2018-07-20 인제대학교 산학협력단 조혈모세포 또는 전구 세포의 체외 확대 성장을 위한 스캐폴드 어셈블리, 상기 스캐폴드 어셈블리를 이용하는 조혈모세포 또는 전구 세포의 체외 확대 성장을 위한 관류형 생물 반응기 및 생물 반응 시스템
WO2013052836A1 (en) * 2011-10-07 2013-04-11 Atmi Packaging, Inc. Fluid processing control system and related methods
US10428309B2 (en) 2011-12-01 2019-10-01 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells
US20140329297A1 (en) * 2011-12-01 2014-11-06 Joule Unlimited Technologies, Inc. Polymeric Thin-Film Tube Connectors, Bioreactors, Systems and Methods
JP6257520B2 (ja) * 2011-12-01 2018-01-10 ニューヨーク ステム セル ファウンデーション インコーポレイテッド 人工多能性幹細胞または分化細胞を作製するための自動化されたシステム
EP2785825B1 (en) * 2011-12-03 2021-04-21 EMD Millipore Corporation Microfluidic cell culture system
US9606035B2 (en) 2011-12-21 2017-03-28 Ta Instruments-Waters Llc System for mechanical stimulation and characterization of biologic samples
CA2920957A1 (en) * 2012-08-28 2014-03-06 Biovest International, Inc. Biomanufacturing suite and methods for large-scale production of cells, viruses, and biomolecules
CN103074213B (zh) * 2012-12-27 2015-03-11 北京航空航天大学 一种用于组织工程反应器的活细胞检测装置及检测方法
WO2014110300A1 (en) * 2013-01-09 2014-07-17 Harvard Apparatus Regenerative Technology Synthetic scaffolds
US10125343B2 (en) 2013-03-13 2018-11-13 Association For The Advancement Of Tissue Engineering And Cell Based Technologies And Therapies—A4Tec Rotational dual chamber bioreactor: methods and uses thereof
US20150031066A1 (en) * 2013-07-26 2015-01-29 Union Biometrica, Inc. Systems, methods, and apparatus for sample dispersion
KR101465362B1 (ko) * 2013-09-11 2014-11-25 성균관대학교산학협력단 조골세포 분화 촉진용 바이오리액터 시스템 및 이를 이용한 조골세포 배양 방법
US9040339B2 (en) 2013-10-01 2015-05-26 The Pen Practical method of producing an aerogel composite continuous thin film thermoelectric semiconductor material
US9276190B2 (en) 2013-10-01 2016-03-01 The Pen Practical method of producing an aerogel composite continuous thin film thermoelectric semiconductor material by modified MOCVD
CN105793411B (zh) 2013-11-16 2018-04-17 泰尔茂比司特公司 生物反应器中的细胞扩增
JP6783143B2 (ja) 2014-03-25 2020-11-11 テルモ ビーシーティー、インコーポレーテッド 培地の受動的補充
CN106232801A (zh) * 2014-04-28 2016-12-14 维瓦生物细胞股份公司 自动化细胞培养和收获装置
US10359415B2 (en) * 2014-05-02 2019-07-23 Rosemount Inc. Single-use bioreactor sensor architecture
US9308296B2 (en) 2014-05-05 2016-04-12 Warsaw Orthopedic, Inc. Tissue processing apparatus and method
US9988599B2 (en) 2014-08-25 2018-06-05 Reviticell Holdings, Inc. Modular single-use kits and methods for preparation of biological material
US20160090569A1 (en) 2014-09-26 2016-03-31 Terumo Bct, Inc. Scheduled Feed
WO2016183143A1 (en) 2015-05-11 2016-11-17 The Trustees Of Columbia University Inthe City Of New York Engineered adult-like human heart tissue
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
EP3349688A4 (en) * 2015-09-15 2019-05-29 University Of Virginia Patent Foundation BIOREACTOR AND NACHSAATKAMMERSYSTEM AND CORRESPONDING METHOD THEREOF
NZ741377A (en) 2015-10-09 2022-02-25 Deka Products Lp Fluid pumping and bioreactor system
WO2017096207A1 (en) * 2015-12-03 2017-06-08 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Modular bioreactor for culture of biopaper based tissues
US11512277B2 (en) 2015-12-22 2022-11-29 Global Life Sciences Solutions Usa Llc Instruments for processing cells
CN121674195A (zh) * 2015-12-22 2026-03-17 环球生命科技咨询美国有限责任公司 在细胞收获设备中以及与其相关的改进
WO2017109072A1 (en) * 2015-12-22 2017-06-29 Ge Healthcare Bio-Sciences Corp. Improvements in and relating to cell harvesting apparatus
WO2017109085A1 (en) * 2015-12-22 2017-06-29 Ge Healthcare Bio-Sciences Corp. Improvements in and relating to cell harvesting apparatus
US12065631B2 (en) 2015-12-22 2024-08-20 Global Life Sciences Solutions Usa Llc Cell harvesting apparatus
KR101807593B1 (ko) * 2015-12-30 2017-12-11 포항공과대학교 산학협력단 흉터 최소화 상처 재생 촉진용 재조합 폴리펩타이드 및 이를 포함하는 생체 접착 소재
US10091986B2 (en) * 2016-05-09 2018-10-09 Xor-Labs Toronto Inc. Organ perfusion device and method
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
WO2018013851A1 (en) 2016-07-13 2018-01-18 The Trustees Of Columbia University Bioreactor system for engineering tissues
US10150115B2 (en) * 2016-07-21 2018-12-11 Spacepharma SA System and method for rehydrating powder and delivering the rehydrated powder to a reactor
US10913930B2 (en) 2016-08-09 2021-02-09 Warsaw Orthopedic, Inc. Tissue processing apparatus and method for infusing bioactive agents into tissue
KR102402037B1 (ko) 2016-08-21 2022-05-24 아드바 바이오테크놀로지 리미티드 생물반응기 및 이의 사용 방법
CN115044471B (zh) 2016-08-27 2025-05-27 三维生物科技有限公司 生物反应器
US11299705B2 (en) 2016-11-07 2022-04-12 Deka Products Limited Partnership System and method for creating tissue
EP3535383B1 (en) * 2016-11-07 2023-09-06 DEKA Products Limited Partnership Method for creating tissue
US10406252B2 (en) 2017-01-19 2019-09-10 Curium Us Llc Systems and methods for autoclave cart loading and unloading system
CN110392733B (zh) * 2017-02-27 2021-02-26 田边刚士 体细胞制造系统
US12234441B2 (en) 2017-03-31 2025-02-25 Terumo Bct, Inc. Cell expansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
IL301152A (en) * 2017-04-07 2023-05-01 Epibone Inc System and method for seeding and culturing
CN110709501A (zh) * 2017-05-04 2020-01-17 苏黎世大学 细胞培养装置
US11649424B2 (en) 2017-07-28 2023-05-16 The Trustees Of Columbia University In The City Of New York Smart micro bioreactor platform for high throughput mechanical stimulation of cardiac microtissue
US11643667B2 (en) 2017-08-28 2023-05-09 Cellino Biotech, Inc. Microfluidic laser-activated intracellular delivery systems and methods
CA3248436A1 (en) 2017-09-01 2025-02-21 Lonza Walkersville, Inc. END-TO-END AUTOMATION OF CELL THERAPY
US10247724B1 (en) 2017-09-28 2019-04-02 Autobiologic Inc. Optically clear sealable petri dish bioreactor
US10696961B2 (en) 2017-12-01 2020-06-30 Global Life Sciences Solutions Usa Llc Magnetic cell isolation techniques
CN111971382A (zh) * 2018-02-09 2020-11-20 环球生命科技咨询美国有限责任公司 用于细胞疗法的生物处理方法
US11414639B2 (en) 2018-02-09 2022-08-16 Global Life Sciences Solutions Usa Llc Bioprocessing vessel
US11920119B2 (en) 2018-02-09 2024-03-05 Global Life Sciences Solutions Usa Llc Systems and methods for bioprocessing
US12252682B2 (en) 2018-02-09 2025-03-18 Global Life Sciences Solutions Usa Llc System and method for fluid flow management in a bioprocessing system
US10889792B2 (en) 2018-02-09 2021-01-12 Global Life Sciences Solutions Usa Llc Cell expansion vessel systems and methods
US11932842B2 (en) 2018-02-09 2024-03-19 Global Life Sciences Solutions Usa Llc Bioprocessing apparatus
US12077743B2 (en) 2018-02-09 2024-09-03 Global Life Sciences Solutions Usa Llc Apparatus for fluid line management in a bioprocessing system
US11371007B2 (en) 2018-02-09 2022-06-28 Global Life Sciences Solutions Usa Llc System and method for fluid flow management in a bioprocessing system
WO2019241499A1 (en) 2018-06-13 2019-12-19 Miromatrix Medical Inc. Fistula filler and deployment system
EP3818142A4 (en) * 2018-07-03 2022-04-27 Advanced Solutions Life Sciences, LLC MODULAR STORAGE UNITS FOR PERFUSION AND/OR INCUBATION OF ONE OR MORE SAMPLES AND STORAGE ARRANGEMENTS
EP3830244A4 (en) 2018-07-27 2022-04-27 The Trustees of Columbia University in the City of New York Human organ-on-chip models for predictive screening
WO2020056033A1 (en) * 2018-09-14 2020-03-19 Pearson Stephen John Large scale cell growing devices and systems
GB201815798D0 (en) 2018-09-27 2018-11-14 Ge Healthcare Bio Sciences Ab System and method for a pharmaceutical product
JP7396777B2 (ja) 2018-09-28 2023-12-12 オクタン バイオテック インコーポレーテッド 磁気分離
CN113166697B (zh) * 2018-10-10 2025-07-15 斯塔姆韦格公司 连续流动式微型生物反应器
WO2020097083A1 (en) 2018-11-06 2020-05-14 Cellino Biotech, Inc. Systems for cell control
CN113330106A (zh) * 2018-12-11 2021-08-31 隆萨沃克斯维尔股份有限公司 用于在自动化生物反应器中使用的细胞分离
JP7477091B2 (ja) 2018-12-21 2024-05-01 オクタン バイオテック インコーポレーテッド モジュラー生物製剤生産ユニットのためのカルーセル
CA3123449A1 (en) 2018-12-21 2020-06-25 Lonza Walkersville, Inc. Automated production of viral vectors
KR102810534B1 (ko) 2018-12-28 2025-05-22 옥테인 바이오테크 인코포레이티드 제어된 환경 존을 포함하는 세포 배양 및 조직 공학 시스템
CN113498432A (zh) 2019-02-08 2021-10-12 隆萨沃克斯维尔股份有限公司 用于自动化生物反应器的细胞浓缩方法和装置
WO2020257335A1 (en) * 2019-06-17 2020-12-24 Deka Products Limited Partnership System and method for centralized fluid management and culture control
JP7698625B2 (ja) * 2019-07-15 2025-06-25 ロンザ ウォーカーズヴィル，インコーポレーテッド 自動細胞工学システム用のプロセス制御システム
EP4023747A4 (en) * 2019-08-29 2023-11-08 Fanuc Corporation CELL PRODUCTION APPARATUS AND SYSTEM THEREOF
JP7297272B2 (ja) * 2019-08-29 2023-06-26 ファナック株式会社 細胞製造装置
US20230038829A1 (en) * 2019-08-29 2023-02-09 Fanuc Corporation Cell production device and cell production method
US11272996B2 (en) 2019-10-04 2022-03-15 Reviticell Holdings, Inc. Methods and devices for performing sequential procedures utilizing a standardized system
IL292409A (en) 2019-10-24 2022-06-01 Octane Biotech Inc A cell culture chamber with enhanced cell contact surfaces
KR20220097457A (ko) 2019-11-11 2022-07-07 론자 워커스빌 아이엔씨. 자동화된 세포 처리를 위한 품질 관리 방법
CA3157773A1 (en) * 2019-11-15 2021-05-20 Christopher Breuer Systems and methods producing seeded grafts
MX2022005577A (es) * 2019-11-22 2022-08-19 Cutiss Ag Metodos y sistemas para producir injertos de piel.
WO2021119486A1 (en) * 2019-12-11 2021-06-17 Humacyte, Inc. Systems, devices and methods for engineered tissue construct transport and containment
CA3168700A1 (en) 2020-01-20 2021-07-29 Adva Biotechnology Ltd. Device and method for controlling a bioreactor
CN115362043B (zh) 2020-02-03 2025-10-17 斯塔姆韦格公司 用于3d打印的平台、系统和设备
WO2021183687A2 (en) 2020-03-10 2021-09-16 Cellares Corporation Systems, devices, and methods for cell processing
JP2023524639A (ja) * 2020-04-22 2023-06-13 アイオバンス バイオセラピューティクス，インコーポレイテッド 患者特異的免疫療法用細胞の製造調整システム及び調整方法
EP4143295A1 (en) 2020-05-01 2023-03-08 DEKA Products Limited Partnership System and method for centralized fluid management and culture control
US20230203418A1 (en) * 2020-05-29 2023-06-29 Univercells Technologies Sa System and method for the production of biomolecules
TWI765297B (zh) * 2020-07-15 2022-05-21 致茂電子股份有限公司 核酸萃取磁棒套之安裝和脫離方法及設備
DE102020126038A1 (de) 2020-07-16 2022-01-20 S-Biosystems Gmbh Vorrichtung zur Gewährleistung einer sterilen Umgebung für die Inkubation von Zellkulturen
AU2021327171B2 (en) * 2020-08-21 2024-09-12 Merck Patent Gmbh Process system for bioreactor-based clean meat production
KR102618894B1 (ko) * 2020-08-28 2023-12-29 주식회사 씨위드 배양육 제조를 위한 세포 배양 장치
WO2022060963A1 (en) 2020-09-17 2022-03-24 Lonza Sales Ag Automated downstream processing of a biologic product
US20220081660A1 (en) * 2020-09-17 2022-03-17 Carnegie Mellon University Shape-Complementing, Porosity-Matching Perfusion Bioreactor System for Engineering Geometrically Complex Tissue Grafts
WO2022094613A1 (en) * 2020-10-29 2022-05-05 Renovacare Sciences Corp. Cell isolation device and methods of use
EP4256025A4 (en) * 2020-12-04 2025-09-03 Sciperio Inc CONTINUOUS EXPANSION VOLUME BIOREACTOR
CN218932173U (zh) * 2021-01-20 2023-04-28 德卡产品有限公司 用于生产线的模块化可配置生物反应器系统
IT202100004313A1 (it) * 2021-02-24 2022-08-24 Univ Degli Studi Genova Apparato biocompatibile e sistema biocompatibile per la creazione di colture cellulari in vitro
CA3210010A1 (en) 2021-03-07 2022-09-15 Matthias Wagner Platforms and systems for automated cell culture
EP4314244B1 (en) 2021-03-23 2025-07-23 Terumo BCT, Inc. Cell capture and expansion
EP4314235A1 (en) * 2021-03-31 2024-02-07 Corning Incorporated Cell culture device sensor caps, systems, and methods
US11931737B2 (en) 2021-09-02 2024-03-19 Cellino Biotech, Inc. Platforms and systems for automated cell culture
CN113984853B (zh) * 2021-09-29 2024-03-01 广东省科学院健康医学研究所 一种用于体外降解和模拟矿化的设备及其使用方法
AU2022368868A1 (en) * 2021-10-18 2024-05-30 Miromatrix Medical Inc. Extracorporeal organ support system
US20240415111A1 (en) * 2021-10-18 2024-12-19 Miromatrix Medical Inc. System for supporting biologically engineered organs
US12545878B2 (en) 2021-12-29 2026-02-10 Bruker Cellular Analysis, Inc. Systems and apparatuses for cellular therapeutics manufacture
US12152699B2 (en) 2022-02-28 2024-11-26 Terumo Bct, Inc. Multiple-tube pinch valve assembly
US20240052285A1 (en) * 2022-08-15 2024-02-15 The Trustees Of The Stevens Institute Of Technology Imaging-enabled bioreactor for in vitro cultivation and bioengineering of isolated airway tissue
USD1099116S1 (en) 2022-09-01 2025-10-21 Terumo Bct, Inc. Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device
US12180453B2 (en) 2023-03-21 2024-12-31 Cellares Corporation Systems, devices, and methods for electroporation within a cell processing system
US12399193B2 (en) 2023-05-05 2025-08-26 Cellares Corporation Systems, devices, and methods for combined cell processes
WO2025007051A2 (en) 2023-06-30 2025-01-02 Cellares Corporation Systems, devices, and methods for fluid transfer within an automated cell processing system
US12497587B2 (en) 2023-08-21 2025-12-16 Cellares Corporation Bioreactors and methods of their use in automatic cell processing systems
WO2025041046A1 (en) 2023-08-21 2025-02-27 Cellares Corporation Systems, devices, and methods for fluid control in a cell processing system
WO2025041064A2 (en) 2023-08-21 2025-02-27 Cellares Corporation Systems, devices, and methods for automatic cell sorting
US12492368B2 (en) 2024-03-11 2025-12-09 Cellares Corporation Monitoring air pressure within a cell processing system
WO2025202944A2 (en) 2024-03-27 2025-10-02 Cellares Corporation Liquid level and flow rate detection within a cell processing system
USD1109621S1 (en) 2024-04-15 2026-01-20 Cellares Corporation Analytical platform for biological material testing
WO2026010918A1 (en) * 2024-07-01 2026-01-08 Ronawk, Inc. Tissue generation for drug discovery and bio-therapeutic modeling in human models

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1356794A (en) * 1970-11-20 1974-06-12 Defence Secratary Of State For Apparatus and method for the production of a single cell suspen sion from tissue
IL68218A (en) * 1983-03-23 1985-12-31 Univ Ramot Compositions for cartilage repair comprising embryonal chondrocytes
US5902741A (en) 1986-04-18 1999-05-11 Advanced Tissue Sciences, Inc. Three-dimensional cartilage cultures
IL82746A (en) 1986-06-06 1994-10-07 Genentech Inc Process for producing biologically active tissue-type plasminogen activator
US5041138A (en) * 1986-11-20 1991-08-20 Massachusetts Institute Of Technology Neomorphogenesis of cartilage in vivo from cell culture
US5081036A (en) * 1987-01-23 1992-01-14 Hoffmann-La Roche Inc. Method and apparatus for cell culture
EP0345588A1 (de) 1988-06-03 1989-12-13 Gesellschaft für Biotechnologische Forschung mbH (GBF) Verfahren und Anordnung zur Regelung eines biologischen Wachstumsprozesses
CA2000181A1 (en) 1988-10-14 1990-04-14 Chung-Faye Chao Process for producing cultured epidermal sheet, product and method of use
JPH02119772A (ja) * 1988-10-29 1990-05-07 Shimadzu Corp 細胞培養装置
DE69029856T2 (de) * 1989-06-15 1997-09-04 Univ Michigan Verfahren, zubereitungen und anordnungen für zellzucht
DE4021123A1 (de) * 1989-10-07 1991-04-11 Sartorius Gmbh Fuer den laborbetrieb bestimmte vorrichtung zur behandlung fluessiger medien
BR9007742A (pt) 1989-10-10 1992-08-11 Aquasearch Inc Processo e aparelho para a cultura de microbios foto-sinteticos e de celulas de macrofitos foto-sinteticos
US5061036A (en) 1990-04-17 1991-10-29 Photon Imaging Corp. Color page scanner using fiber optic bundle and a photosensor array
GB9010356D0 (en) * 1990-05-09 1990-06-27 Ciba Geigy Ag Maturation of hemopoietic cells
EP0597964A4 (en) 1991-08-07 1994-11-30 Einstein Coll Med PROLIFERATION OF HEPATOCYTE PRECURSORS.
AU685743B2 (en) * 1992-03-04 1998-01-29 Regents Of The University Of Michigan, The Methods, compositions and devices for maintaining and growing human stem and/or hematopoietic cells
JPH0654678A (ja) 1992-08-07 1994-03-01 Chuo Setsubi Eng Kk 微生物の自動流加培養法
DE4306661C2 (de) 1993-03-03 1995-04-20 Michael Dipl Biol Sittinger Verfahren zum Herstellen eines Implantates aus Zellkulturen
JP3453161B2 (ja) 1993-03-18 2003-10-06 宇宙開発事業団 液体培養ユニット
US5424209A (en) * 1993-03-19 1995-06-13 Kearney; George P. Automated cell culture and testing system
US5549134A (en) * 1994-05-27 1996-08-27 Marcvalve Corporation Diaphragm valve
US5589334A (en) 1994-06-03 1996-12-31 Ludwig Institute For Cancer Research Isolated nucleic acid molecule which codes for a tumor rejection antigen precursor which is processed to an antigen presented by HLA-B44, and uses thereof
JP3549949B2 (ja) * 1994-06-17 2004-08-04 山陽電子工業株式会社 低酸素培養器
US5906934A (en) * 1995-03-14 1999-05-25 Morphogen Pharmaceuticals, Inc. Mesenchymal stem cells for cartilage repair
US5627729A (en) 1995-04-03 1997-05-06 Methode Electronics, Inc. One-piece memory card
US5846828A (en) * 1995-06-07 1998-12-08 Advanced Tissue Sciences Apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing tissue, synthetic, or mechanical heart valves orvalve segments
AU703117B2 (en) * 1995-04-27 1999-03-18 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native vascular grafts
US6121042A (en) 1995-04-27 2000-09-19 Advanced Tissue Sciences, Inc. Apparatus and method for simulating in vivo conditions while seeding and culturing three-dimensional tissue constructs
US5792603A (en) * 1995-04-27 1998-08-11 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts
WO1996040858A1 (en) * 1995-06-07 1996-12-19 Aastrom Biosciences, Inc. Apparatus and method for maintaining and growing biological cells
US6238908B1 (en) * 1995-06-07 2001-05-29 Aastrom Biosciences, Inc. Apparatus and method for maintaining and growth biological cells
US5688687A (en) * 1995-06-07 1997-11-18 Aastrom Biosciences, Inc. Bioreactor for mammalian cell growth and maintenance
US6228635B1 (en) * 1995-06-07 2001-05-08 Aastrom Bioscience, Inc. Portable cell growth cassette for use in maintaining and growing biological cells
US5728581A (en) * 1995-06-07 1998-03-17 Systemix, Inc. Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein
WO1996039992A1 (en) * 1995-06-07 1996-12-19 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing replacement cartilage tissue constructs
JP4465046B2 (ja) * 1995-09-01 2010-05-19 ワルソー・オーソペディック，インコーポレイテッド 特に骨細胞活性を支持する様に調整されたリン酸カルシウム相の人工安定化組成物
CA2244659C (en) * 1995-10-06 2007-05-01 Leonardus Marcus Flendrig Biological reactor for the cultivation of cells
WO1997017426A1 (en) * 1995-11-08 1997-05-15 Trustees Of Boston University Cellular physiology workstations for automated data acquisition and perfusion control
US5842477A (en) 1996-02-21 1998-12-01 Advanced Tissue Sciences, Inc. Method for repairing cartilage
US5827729A (en) * 1996-04-23 1998-10-27 Advanced Tissue Sciences Diffusion gradient bioreactor and extracorporeal liver device using a three-dimensional liver tissue
ES2273404T3 (es) * 1997-01-31 2007-05-01 Schering Corporation Metodos para cultivar celulas y propagar virus.
US5786207A (en) * 1997-05-28 1998-07-28 University Of Pittsburgh Tissue dissociating system and method
EP1015558A1 (en) * 1997-09-25 2000-07-05 Cytomatrix, LLC Methods and devices for the long-term culture of hematopoietic progenitor cells
KR100652511B1 (ko) * 1998-01-29 2006-11-30 밀레늄 바이올로직스 인코포레이티드 합성 생체 물질 화합물
US6197575B1 (en) 1998-03-18 2001-03-06 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
US5882929A (en) * 1998-04-07 1999-03-16 Tissue Engineering, Inc. Methods and apparatus for the conditioning of cartilage replacement tissue
US5989913A (en) * 1998-07-02 1999-11-23 Charles Daniel Anderson Culture vessel for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using the same
MXPA01007820A (es) 1999-02-04 2003-06-19 Technion Res & Dev Foundation Metodo y aparato para mantenimiento y expansion de celulas de tallo hemopoyeticas y/o celulas progenitoras.
AU5767900A (en) * 1999-06-25 2001-01-31 Advanced Tissue Sciences, Inc. Monitorable three-dimensional scaffolds and tissue culture systems
US6632651B1 (en) * 1999-07-06 2003-10-14 Ramot At Tel Aviv University Ltd. Tissue maintenance system that applies rhythmic pulses of pressure
JP3865354B2 (ja) * 2000-03-02 2007-01-10 高木産業株式会社 細胞又は組織の培養方法
JP4402249B2 (ja) * 2000-03-31 2010-01-20 正仁 田谷 細胞培養方法、細胞培養装置及び記録媒体
US7585323B2 (en) * 2000-05-05 2009-09-08 Medlden, Llc In vitro mechanical loading of musculoskeletal tissues
US6485963B1 (en) * 2000-06-02 2002-11-26 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Growth stimulation of biological cells and tissue by electromagnetic fields and uses thereof
EP1174497B1 (fr) * 2000-07-19 2004-09-08 Technodop Ltd. (Société de Droit Irlandais) Chambre de culture cellulaire et bioréacteur pour la culture extracorporelle de cellules animales
EP1309719B2 (en) * 2000-08-14 2022-09-07 University of Maryland at Baltimore County Bioreactor and bioprocessing technique
JP4195287B2 (ja) 2000-10-02 2008-12-10 エフ．キャノン トーマス 自動化されたバイオカルチャー及びバイオカルチャー実験システム
US20020146817A1 (en) 2000-10-02 2002-10-10 Cannon Thomas F. Automated bioculture and bioculture experiments system
US6673595B2 (en) * 2001-08-27 2004-01-06 Biocrystal, Ltd Automated cell management system for growth and manipulation of cultured cells
GB0121986D0 (en) 2001-09-11 2001-10-31 Isis Innovation Method and structure for growing living organic tissue
US7595043B2 (en) * 2001-12-07 2009-09-29 Cytori Therapeutics, Inc. Method for processing and using adipose-derived stem cells

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