DK2371949T3 - Lipasevarianter - Google Patents

Lipasevarianter Download PDF

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Publication number
DK2371949T3
DK2371949T3 DK10180310.4T DK10180310T DK2371949T3 DK 2371949 T3 DK2371949 T3 DK 2371949T3 DK 10180310 T DK10180310 T DK 10180310T DK 2371949 T3 DK2371949 T3 DK 2371949T3
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Denmark
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ala
gly
thr
ser
val
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DK10180310.4T
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Jesper Vind
Jürgen Carsten Franz Knötzel
Kim Borch
Allan Svendsen
Thomas Honger Callisen
Debbie Yaver
Mads Eskelund Bjoernvad
Peter Kamp Hansen
Haiyan Ge
Michael Lamsa
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Novozymes As
Novozymes Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Developing Agents For Electrophotography (AREA)
  • Beverage Vending Machines With Cups, And Gas Or Electricity Vending Machines (AREA)
  • Slot Machines And Peripheral Devices (AREA)

Claims (14)

1. Variant af en stamlipase, hvor varianten har lipaseaktivitet, er mindst 80% identisk med SEQ ID NO: 2 og omfatter substitutionerne Q4V +S58A +V60S +S83T +I86V +A150G +E210K +L227G +T231R +N233R +P256K (under anvendelse af SEQ ID NO: 2 til nummerering).
2. Lipasevariant ifølge krav 1, hvor stamlipasen er lipasen med aminosyresekvensen ifølge SEQ ID NO: 2.
3. Lipasevariant ifølge krav 1, hvor lipasevarianten endvidere omfatter substitutioner valgt fra gruppen bestående af substitutioner i positioner svarende til positionerne 202, 211 og 255 (under anvendelse af SEQ ID NO: 2 til nummerering).
4. Lipasevariant ifølge krav 3, hvor substitutionerne er X202G, X211L og X255Y/V.
5. Lipasevariant ifølge krav 1, hvor lipasevarianten endvidere omfatter en substitution i en position svarende til position 90 (under anvendelse af SEQ ID NO: 2 til nummerering).
6. Lipasevariant ifølge krav 5, hvor substitutionen er X90A/R.
7. Lipasevariant ifølge krav 1, hvor lipasevarianten endvidere omfatter en substitution i en position svarende til position 27 (under anvendelse af SEQ ID NO: 2 til nummerering).
8. Lipasevariant ifølge krav 7, hvor substitutionen er X27R.
9. Lipasevariant ifølge krav 1, hvor lipasevarianten endvidere omfatter mindst én substitution i stamlipasen valgt fra gruppen bestående af substitutioner i positioner svarende til position 81, 147 og 249 (under anvendelse af SEQ ID NO: 2 til nummerering).
10. Lipasevariant ifølge krav 9, hvor substitutionerne er X81Q/E, X147M/Y og X249R/I/L.
11. DNA-sekvens, som koder for lipasevarianten ifølge krav 1-10.
12. Ekspressionsvektor, som huser DNA-sekvensen ifølge krav 11.
13. Transformeret værtscelle, som indeholder DNA-sekvensen ifølge krav 11.
14. Fremgangsmåde til fremstilling af en lipasevariant, hvilken fremgangsmåde omfatter dyrkning af den transformerede værtscelle ifølge krav 13 under betingelser, som er befordrende for fremstilling af lipasevarianten, og indvinding af lipasevarianten fra det resulterende medium.
DK10180310.4T 2006-01-23 2007-01-22 Lipasevarianter DK2371949T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US76110906P 2006-01-23 2006-01-23
US85489106P 2006-10-27 2006-10-27
EP07717346.6A EP1979477B1 (en) 2006-01-23 2007-01-22 Lipase variants

Publications (1)

Publication Number Publication Date
DK2371949T3 true DK2371949T3 (da) 2017-06-26

Family

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Family Applications (3)

Application Number Title Priority Date Filing Date
DK10180288.2T DK2371948T5 (da) 2006-01-23 2007-01-22 Lipasevarianter
DK07717346.6T DK1979477T3 (da) 2006-01-23 2007-01-22 Lipasevarianter
DK10180310.4T DK2371949T3 (da) 2006-01-23 2007-01-22 Lipasevarianter

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DK10180288.2T DK2371948T5 (da) 2006-01-23 2007-01-22 Lipasevarianter
DK07717346.6T DK1979477T3 (da) 2006-01-23 2007-01-22 Lipasevarianter

Country Status (8)

Country Link
US (1) US8187854B2 (da)
EP (3) EP2371948B1 (da)
JP (1) JP5133899B2 (da)
CN (2) CN101370933B (da)
BR (1) BRPI0707202A2 (da)
DK (3) DK2371948T5 (da)
ES (3) ES2628940T3 (da)
WO (1) WO2007087508A2 (da)

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