DK2374892T3 - Ekspression af virusproteiner i planter - Google Patents
Ekspression af virusproteiner i planter Download PDFInfo
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- DK2374892T3 DK2374892T3 DK11162685.9T DK11162685T DK2374892T3 DK 2374892 T3 DK2374892 T3 DK 2374892T3 DK 11162685 T DK11162685 T DK 11162685T DK 2374892 T3 DK2374892 T3 DK 2374892T3
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- plant
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- vector
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- influenza virus
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- 239000008223 sterile water Substances 0.000 description 1
- 229940031626 subunit vaccine Drugs 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000012250 transgenic expression Methods 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 230000021542 voluntary musculoskeletal movement Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/08—RNA viruses
- C07K14/11—Orthomyxoviridae, e.g. influenza virus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Communicable Diseases (AREA)
- Pulmonology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (13)
- Ekspression af virusproteiner i planter Patentkrav1. Fremgangsmåde til produktion af et influenzavirus-H5-polypeptid i en plante omfattende trinnene med: i) kloning af et influenzavirus-H5-gen eller en nukleinsyre, der koder for et H5-polypeptid, ind i en vektor, der er tilpasset til at målrettes en komponent, der er til stede i planten, hvori plantekomponenten er det endoplasmatiske retikulum; ii) infiltration af mindst en del af planten med vektoren eller transformation af plantevæv med vektoren, således at influenzavirus-H5-polypeptidet udtrykkes transitorisk; og iii) indvinding af det influenza-virus-H5-polypeptid, som planten har udtrykt.
- 2. Fremgangsmåde ifølge krav 1, hvori en eller flere målretningssekvenser, der koder for et polypeptid til at føre influenza-virus-H5-polypeptidet fra cytoplasmaet til plantekomponenten, er inkluderet i vektoren.
- 3. Fremgangsmåde ifølge krav 1 eller krav 2, hvori vektoren inkluderer promotere og andre regulatorer eller lignende, der er funktionelt koblet til vektorens kodnings sekvens.
- 4. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori vektorerne er binære vektorer, fortrinsvis binære vektorer fra Agrobacterium tumefaciens.
- 5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori influenzavirus-H5-genet er et kodonanvendelsesoptimeret gen, fortrinsvis et kodonanvendelsesoptimeret humant gen, kodonanvendelsesoptimeret BCG-gen eller kodonanvendelsesoptimeret plantegen.
- 6. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, endvidere omfattende et trin med co-infiltration af planten med et suppressorprotein tilpasset til hæmning af post-transkriptional gen-silencing i en plante såsom NSs-proteinet fra tomatbronzetopvirus eller p 19 fra tombusvirus.
- 7. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori infiltrationen foretages ved direkte injektion eller ved vakuum.
- 8. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori infiltration og/eller transformation af planten opnås med Agrobacterium tumefaciens, der er blevet transformeret til at acceptere vektoren.
- 9. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori planten er udvalgt fra Nicotiana benthamiana og N. tabacum.
- 10. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori influenzavirus-H5-genet eller nukleinsyren, der koder for en funktionel ækvivalent dertil er udvalgt fra sekvenserne som fremlagt i figur 10 og 11.
- 11. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori infiltration foretages på plantens blade, fortrinsvis hvori infiltration foretages ved direkte injektion på bladets abaksiale region.
- 12. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 10, hvori i det væsentlige hele planten infiltreres med en egnet vektor ved hjælp af vakuuminfiltration.
- 13. Anvendelse af en vektor, ind i hvilken et influenzavirus-H5-gen eller en nukleinsyre, der koder for et H5-polypeptid, er blevet klonet, hvilken vektor er tilpasset til at målrette H5-polypeptidet det endoplasmatiske retikulum i en plante, således at influenzavirus-H5-polypeptidet udtrykkes transitorisk i cytoplasmaet, og influenzavirus-H5-polypeptidet derefter importeres ind i det endoplasmatiske retikulum.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ZA200503454 | 2005-04-29 | ||
| EP06740997A EP1883701B1 (en) | 2005-04-29 | 2006-05-02 | Expression of viral proteins in plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2374892T3 true DK2374892T3 (da) | 2018-03-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| DK11162685.9T DK2374892T3 (da) | 2005-04-29 | 2006-05-02 | Ekspression af virusproteiner i planter |
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| US (2) | US8535930B2 (da) |
| EP (2) | EP2374892B1 (da) |
| CN (2) | CN101166827B (da) |
| AT (1) | ATE541938T1 (da) |
| DK (1) | DK2374892T3 (da) |
| ES (2) | ES2662118T3 (da) |
| PL (2) | PL2374892T3 (da) |
| PT (1) | PT2374892T (da) |
| WO (1) | WO2006119516A2 (da) |
| ZA (2) | ZA200803486B (da) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007040568A2 (en) * | 2004-12-01 | 2007-04-12 | Arizona Board Of Regents, Acting For And On Behalf Of Arizona State Univeristy | Production and use of human butyrylcholinesterase |
| CA3184778A1 (en) * | 2006-07-28 | 2008-01-31 | The Trustees Of The University Of Pennsylvania | Hiv consensus envelope sequences and methods for using same |
| WO2008091657A1 (en) * | 2007-01-23 | 2008-07-31 | Academia Sinica | Flu vaccines and methods of use thereof |
| CA2615372A1 (en) | 2007-07-13 | 2009-01-13 | Marc-Andre D'aoust | Influenza virus-like particles (vlps) comprising hemagglutinin |
| KR101255419B1 (ko) * | 2007-11-12 | 2013-04-17 | 이노비오 파마수티컬즈, 인크. | 인플루엔자 바이러스의 다중 아형에 대한 신규 백신 |
| NZ587108A (en) | 2007-11-27 | 2013-04-26 | Medicago Inc | Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin |
| US7981428B2 (en) * | 2008-01-23 | 2011-07-19 | Academia Sinica | Flu vaccines and methods of use thereof |
| US10689633B2 (en) | 2008-02-29 | 2020-06-23 | The Trustees Of The University Of Pennsylvania | Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis |
| DK2294202T3 (da) | 2008-07-08 | 2015-08-31 | Medicago Inc | Opløselige rekombinante influenza-antigener |
| KR101745925B1 (ko) | 2008-07-18 | 2017-06-12 | 메디카고 인코포레이티드 | 신규 인플루엔자 바이러스 면역화 에피토프 |
| SI2445928T1 (en) | 2009-06-24 | 2018-05-31 | Medicago Inc. | HYPER PARTICULARS, SIMILAR VIRUSES OF GRIPE, WHICH HAVE EXPOSED HEMAGLUTININE |
| SG178918A1 (en) | 2009-09-22 | 2012-04-27 | Medicago Inc | Method of preparing plant-derived vlps |
| WO2011077371A1 (en) * | 2009-12-22 | 2011-06-30 | University Of Cape Town | Method for enhancing the expression of hpv l1 |
| CN101892248B (zh) * | 2010-04-29 | 2012-04-18 | 王世霞 | 禽流感h5 ha抗原的单克隆抗体 |
| CN102533722B (zh) * | 2010-12-24 | 2014-03-12 | 北京义翘神州生物技术有限公司 | 一种通过温度突变提高哺乳动物细胞重组蛋白瞬时表达的方法 |
| EP2665817A1 (en) * | 2011-01-17 | 2013-11-27 | Philip Morris Products S.a.s. | Vectors for nucleic acid expression in plants |
| WO2012098119A2 (en) * | 2011-01-17 | 2012-07-26 | Philip Morris Products S.A. | Protein expression in plants |
| TWI620816B (zh) | 2011-03-23 | 2018-04-11 | 苜蓿股份有限公司 | 植物衍生蛋白回收方法 |
| TWI700368B (zh) * | 2011-09-30 | 2020-08-01 | 苜蓿股份有限公司 | 增加植物類病毒顆粒產率 |
| MY184076A (en) | 2011-12-01 | 2021-03-17 | Univ Cape Town | Hpv chimaeric particle |
| US10159884B2 (en) | 2012-11-09 | 2018-12-25 | Wilson Sporting Goods Co. | Basketball make-miss shot sensing |
| US9901801B2 (en) | 2012-11-09 | 2018-02-27 | Wilson Sporting Goods Co. | Basketball sensing apparatus |
| JP2016516415A (ja) | 2013-03-28 | 2016-06-09 | メディカゴ インコーポレイテッド | 植物におけるインフルエンザウイルス様粒子の生成 |
| US12467058B2 (en) | 2013-03-28 | 2025-11-11 | Aramis Biotechnologies Inc. | Influenza virus-like particle production in plants |
| BR112016015875A2 (pt) | 2014-01-10 | 2017-09-19 | Medicago Inc | Elementos intensificadores de cpmv |
| CA2944422A1 (en) * | 2014-04-10 | 2015-10-15 | Mitsubishi Chemical Corporation | Method for producing a useful protein using a plant |
| US9916001B2 (en) | 2014-07-08 | 2018-03-13 | Wilson Sporting Goods Co. | Sport equipment input mode control |
| WO2016004536A1 (en) * | 2014-07-11 | 2016-01-14 | Medicago Inc. | Modifying protein production in plants |
| CN109385445A (zh) * | 2017-08-09 | 2019-02-26 | 北京睿诚海汇健康科技有限公司 | 植物作为宿主在表达贝伐抗体中的应用 |
| CN114106206B (zh) * | 2021-12-02 | 2023-02-28 | 猎境(嘉兴)生物科技有限公司 | 一种利用植物膜系统生产重组疫苗的方法 |
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| US7129391B1 (en) * | 1988-09-26 | 2006-10-31 | Auburn University | Universal chloroplast integration and expression vectors, transformed plants and products thereof |
| US20010053367A1 (en) * | 1991-08-26 | 2001-12-20 | Prodigene, Inc. | Vaccines expressed in plants |
| AU733792B2 (en) * | 1997-01-17 | 2001-05-24 | Scripps Research Institute, The | RNA binding protein and binding site useful for expression of recombinant molecules |
| WO2003018624A1 (en) * | 2001-08-31 | 2003-03-06 | University Of Cape Town | Vectors, constructs, and transgenic plants for hpv-11 and hpv-16 l1 capsid protein |
| DK1506222T3 (da) | 2002-05-17 | 2009-07-06 | Univ Cape Town | Kimæriske, humane papillomavirus16 L1-proteiner omfattende et L2-peptid, viruslignende partikler fremstillet deraf samt en fremgangsmåde til at fremstille partiklerne |
| AU2003253781A1 (en) | 2002-07-03 | 2004-01-23 | University Of Central Florida | Expression of human interferon in transgenic chloroplasts |
| WO2005011367A1 (en) | 2003-07-03 | 2005-02-10 | University Of Central Florida | A chloroplast transgenic approach to express and purify human serum albumin, a protein highly susceptible to proteolytic degradation |
-
2006
- 2006-05-02 ES ES11162685.9T patent/ES2662118T3/es not_active Expired - Lifetime
- 2006-05-02 EP EP11162685.9A patent/EP2374892B1/en not_active Expired - Lifetime
- 2006-05-02 ES ES06740997T patent/ES2381200T3/es not_active Expired - Lifetime
- 2006-05-02 DK DK11162685.9T patent/DK2374892T3/da active
- 2006-05-02 WO PCT/ZA2006/000063 patent/WO2006119516A2/en not_active Ceased
- 2006-05-02 EP EP06740997A patent/EP1883701B1/en not_active Expired - Lifetime
- 2006-05-02 CN CN2006800142025A patent/CN101166827B/zh not_active Expired - Fee Related
- 2006-05-02 PT PT111626859T patent/PT2374892T/pt unknown
- 2006-05-02 PL PL11162685T patent/PL2374892T3/pl unknown
- 2006-05-02 US US11/912,912 patent/US8535930B2/en not_active Expired - Fee Related
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- 2006-05-02 CN CN201210488322.6A patent/CN103031310B/zh not_active Expired - Fee Related
- 2006-05-02 AT AT06740997T patent/ATE541938T1/de active
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- 2008-04-21 ZA ZA2008/03486A patent/ZA200803486B/en unknown
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Also Published As
| Publication number | Publication date |
|---|---|
| EP2374892B1 (en) | 2018-02-14 |
| EP2374892A3 (en) | 2011-12-28 |
| US8535930B2 (en) | 2013-09-17 |
| ES2381200T3 (es) | 2012-05-24 |
| US9017987B2 (en) | 2015-04-28 |
| CN101166827A (zh) | 2008-04-23 |
| US20130130314A1 (en) | 2013-05-23 |
| ATE541938T1 (de) | 2012-02-15 |
| PL2374892T3 (pl) | 2018-06-29 |
| PT2374892T (pt) | 2018-03-29 |
| ZA201204565B (en) | 2014-03-26 |
| ES2662118T3 (es) | 2018-04-05 |
| EP1883701B1 (en) | 2012-01-18 |
| CN103031310B (zh) | 2018-09-14 |
| WO2006119516A2 (en) | 2006-11-09 |
| CN103031310A (zh) | 2013-04-10 |
| ZA200803486B (en) | 2012-09-26 |
| WO2006119516A3 (en) | 2007-06-28 |
| EP1883701A2 (en) | 2008-02-06 |
| US20090220543A1 (en) | 2009-09-03 |
| CN101166827B (zh) | 2013-09-04 |
| PL1883701T3 (pl) | 2012-06-29 |
| EP2374892A2 (en) | 2011-10-12 |
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