DK2456864T3 - Syntetiske oplophorus-luciferaser med forbedret lysemission - Google Patents
Syntetiske oplophorus-luciferaser med forbedret lysemission Download PDFInfo
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- DK2456864T3 DK2456864T3 DK10717013.6T DK10717013T DK2456864T3 DK 2456864 T3 DK2456864 T3 DK 2456864T3 DK 10717013 T DK10717013 T DK 10717013T DK 2456864 T3 DK2456864 T3 DK 2456864T3
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
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Claims (16)
1. Polynukleotid, der koder for et modificeret luciferasepolypeptid med mindst 60 % aminosyresekvensidentitet med en vildtype Oplophorus-\uciferase og omfatter mere end én aminosyresubstitution ved positionerne valgt fra 2, 4, 11, 20, 23, 28, 33, 34, 44, 45, 51, 54, 68, 72, 75, 76, 77, 89, 90, 92, 99, 104, 115, 124, 135, 138, 139, 143, 144, 166, 167 og 169 svarende til SEQ ID NO: 1, hvor det modificerede luciferasepolypeptid har en mindst fire gange større luminescensemission i en prokaryot celle og/eller en eukaryot celle i forhold den tilsvarende vildtype Oplophorus-\uc\ferase.
2. Polynukleotid ifølge krav 1, hvor det kodede modificerede luciferasepolypeptid har forbedret signalstabilitet i forhold til vildtype Oplophorus-\uciferase og en aminosyresubstitution ved mindst én af positionerne 4, 11, 20, 28, 33, 54, 68, 75, 115, 124, 138, 143 eller 166 svarende til SEQ ID NO: 1.
3. Polynukleotid ifølge et hvilket som helst af de foregående krav, hvor det kodede modificerede luciferasepolypeptid har forbedret proteinstabilitet i forhold til vildtype Oplophorus-\uc\ferase og en aminosyresubstitution ved mindst én af positionerne 11, 20, 28, 33, 34, 44, 45, 51, 54, 68, 72, 75, 77, 89, 90, 92, 99, 104, 115, 124, 135, 138, 139, 143, 144, 166, 167 eller 169 svarende til SEQ ID NO: 1.
4. Polynukleotid ifølge et hvilket som helst af de foregående krav, hvor det kodede modificerede luciferasepolypeptid endvidere omfatter en deletion af luciferaserester svarende til N-terminus af vildtype Oplophorus-\uc\feraser\ ifølge SEQ ID NO: 10.
5. Polynukleotid ifølge et hvilket som helst af de foregående krav, hvor vildtype Oplophorus-\uc\ferasen er fra Oplophorus gracilirostris, Oplophorus grimaldii, Oplophorus spinicauda, Oplophorus foliaceus, Oplophorus novaezelandiae, Oplophorus typus eller Oplophorus spinous.
6. Polynukleotid ifølge et hvilket som helst af de foregående krav, hvor det kodede modificerede luciferasepolypeptid omfatter en substitution svarende til mindst én af T2S, A4E/S/R/G/D/T/L, Q11R/V/I/L/K/T, Q20R, E23V, S28P, A33K, L34M, V44I/L, V45E, G51V, A54F/T/V/G/S/W/IVI, F68S/Y/N, L72Q, M75R/K/Q/G/T/A, I76V, F77W, V79I, K89E, I90T/V, L92S/A/G/Q, I99V, P104L, PI 15E/I/Q/IVV/G/H/R/S/C/A/T, Q124K, N135K, Y138V/I/N/T/L/C/R/M/K, D139E, I143L, N144K, N166R/K/A/IVP/Q/S, I167V eller A169L svarende til SEQ ID NO: 1.
7. Polynukleotid ifølge et hvilket som helst af de foregående krav, hvor det kodede modificerede luciferasepolypeptid omfatter aminosyresubstitutioner (i) ved positionerne 11, 33, 44, 54, 115, 124, 138 og 166 svarende til SEQ ID NO: 1, (ii) ved positionerne 23, 28, 143 og 166 svarende til SEQ ID NO: 1, (iii) ved positionerne 4, 34, 76 og 166 svarende til SEQ ID NO: 1 eller (iv) ved positionerne 51, 99 og 166 svarende til SEQ ID NO: 1.
8. Polynukleotid ifølge krav 7 (i), hvor den kodede modificerede luciferase endvidere omfatter en aminosyresubstitution ved position 4 svarende til SEQ ID NO: 1.
9. Polynukleotid ifølge krav 7 (i) eller 8, hvor den kodede modificerede luciferase endvidere omfatter aminosyresubstitutioner ved positionerne 45, 135, 104, 139 og 167 svarende til SEQ ID NO: 1.
10. Polynukleotid ifølge et hvilket som helst af kravene 7 (i), 8 eller 9, hvor den kodede modificerede luciferase endvidere omfatter aminosyresubstitutioner ved positionerne 28, 34, 51, 99 og 143 svarende til SEQ ID NO: 1.
11. Polynukleotid ifølge krav 8, hvilket polynukleotid endvidere omfatter en substitution af mindst én aminosyre ved position 20, 54, 68, 72, 77, 79, 89, 90 eller 92 svarende til SEQ ID NO: 1.
12. Vektor omfattende polynukleotidet ifølge et hvilket som helst af de foregående krav.
13. Vektor ifølge krav 12, hvor polynukleotidet er operativt forbundet med en promoter.
14. Celle omfattende polynukleotidet ifølge et hvilket som helst af kravene 1-11 eller vektoren ifølge krav 12 eller 13.
15. Polypeptid kodet for af polynukleotidet ifølge et hvilket som helst af kravene 1-11.
16. Kit omfattende polynukleotidet ifølge et hvilket som helst af kravene 1-11 eller vektoren ifølge krav 12 eller 13.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17483809P | 2009-05-01 | 2009-05-01 | |
| PCT/US2010/033449 WO2010127368A1 (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with enhanced light output |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2456864T3 true DK2456864T3 (da) | 2015-12-21 |
Family
ID=42358981
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17154777.1T DK3181687T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forøget lysudbytte |
| DK18184699.9T DK3409764T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysudbytte |
| DK10717013.6T DK2456864T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysemission |
| DK15186652.2T DK2990478T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysemission |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17154777.1T DK3181687T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forøget lysudbytte |
| DK18184699.9T DK3409764T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysudbytte |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15186652.2T DK2990478T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysemission |
Country Status (16)
| Country | Link |
|---|---|
| US (7) | US8557970B2 (da) |
| EP (5) | EP2990478B1 (da) |
| JP (2) | JP6038649B2 (da) |
| KR (1) | KR101665352B1 (da) |
| CN (2) | CN102459579B (da) |
| AU (1) | AU2010242771B2 (da) |
| BR (1) | BRPI1010019B1 (da) |
| CA (1) | CA2758572A1 (da) |
| DK (4) | DK3181687T3 (da) |
| ES (5) | ES2795287T3 (da) |
| IL (3) | IL215697B (da) |
| LT (1) | LT3409764T (da) |
| PL (5) | PL2456864T3 (da) |
| PT (4) | PT3181687T (da) |
| SG (3) | SG10201901453PA (da) |
| WO (1) | WO2010127368A1 (da) |
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|---|---|---|---|---|
| LT3409764T (lt) | 2009-05-01 | 2020-06-10 | Promega Corporation | Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga |
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| CN115161296B (zh) * | 2021-04-01 | 2024-08-13 | 深圳华大生命科学研究院 | 一种刺虾荧光素酶Nluc的突变体及其应用 |
| CN113025586B (zh) * | 2021-04-06 | 2022-10-04 | 湖北省中医院 | 一种经改造的萤光素酶突变体蛋白、生物发光探针、探针组、制备方法和检测方法 |
| EP4337646A4 (en) | 2021-05-13 | 2025-05-21 | Promega Corporation | BIOLUMINESCENT DETECTION OF DNA SYNTHESIS |
| US20240060059A1 (en) | 2022-05-04 | 2024-02-22 | Promega Corporation | Circularly permuted dehalogenase variants |
| WO2023215497A1 (en) | 2022-05-04 | 2023-11-09 | Promega Corporation | Photoactivatable compounds and uses thereof |
| JP2025515181A (ja) | 2022-05-04 | 2025-05-13 | プロメガ コーポレイション | 伸長表面ループ領域を有する修飾デハロゲナーゼ |
| EP4519426A2 (en) | 2022-05-04 | 2025-03-12 | Promega Corporation | Split modified dehalogenase variants |
| GB202213479D0 (en) | 2022-09-14 | 2022-10-26 | Ipsen Biopharm Ltd | Cell-free clostridial neurotoxin assays |
| WO2024059832A1 (en) * | 2022-09-15 | 2024-03-21 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods for assessing kinase activity |
| GB202404021D0 (en) | 2024-03-20 | 2024-05-01 | Ipsen Biopharm Ltd | Cell-based neurotoxin assay |
| CN119307519B (zh) * | 2024-07-22 | 2025-11-25 | 苏州剂泰医药科技有限公司 | 编码荧光素酶的mRNA及其应用 |
| CN119709661B (zh) * | 2024-12-30 | 2025-10-10 | 佰翱得(无锡)新药开发有限公司 | 一种荧光素酶OLuc突变体及其应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8911957D0 (en) | 1989-05-24 | 1989-07-12 | Interox Chemicals Ltd | Peroxyacid manufacture |
| US5368484A (en) | 1992-05-22 | 1994-11-29 | Atari Games Corp. | Vehicle simulator with realistic operating feedback |
| EP1978091A1 (en) * | 1994-01-03 | 2008-10-08 | Promega Corporation | Mutant luciferases |
| CA2301828A1 (en) | 1997-09-19 | 1999-03-25 | Promega Corporation | Thermostable luciferases and methods of production |
| JP3694181B2 (ja) * | 1999-01-05 | 2005-09-14 | キッコーマン株式会社 | 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法 |
| NZ518413A (en) * | 1999-10-26 | 2004-11-26 | Secr Defence | Novel enzyme |
| EP1156103B1 (en) | 2000-04-26 | 2010-08-11 | Chisso Corporation | Oplophorus luciferase |
| JP4613441B2 (ja) * | 2000-04-26 | 2011-01-19 | チッソ株式会社 | 新規ルシフェラーゼおよび発光蛋白質 |
| US7118878B1 (en) | 2000-06-09 | 2006-10-10 | Promega Corporation | Method for increasing luminescence assay sensitivity |
| US7268229B2 (en) | 2001-11-02 | 2007-09-11 | Promega Corporation | Compounds to co-localize luminophores with luminescent proteins |
| EP2433871A3 (en) | 2002-06-19 | 2012-07-11 | Medical Instill Technologies, Inc. | Sterile filling machine having needle filling station within e-beam chamber |
| CA2561809A1 (en) * | 2004-03-31 | 2005-10-20 | Xencor, Inc. | Bmp-7 variants with improved properties |
| JP3844082B2 (ja) * | 2005-04-08 | 2006-11-08 | キッコーマン株式会社 | 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び耐熱性ホタルルシフェラーゼの製造法 |
| JP4999341B2 (ja) | 2005-09-06 | 2012-08-15 | 株式会社バイオエネックス | 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法 |
| EP2327768B1 (en) * | 2006-04-03 | 2015-09-09 | Promega Corporation | Permuted and nonpermuted luciferase biosensors |
| US20080248511A1 (en) | 2007-03-26 | 2008-10-09 | Promega Corporation | Methods to quench light from optical reactions |
| EP2215227A2 (en) | 2007-11-05 | 2010-08-11 | Promega Corporation | Hybrid fusion reporter and uses thereof |
| WO2009142735A2 (en) * | 2008-05-19 | 2009-11-26 | Promega Corporation | LUCIFERASE BIOSENSORS FOR cAMP |
| WO2010119721A1 (ja) | 2009-04-17 | 2010-10-21 | 独立行政法人産業技術総合研究所 | 超高輝度で安定な人工生物発光酵素 |
| LT3409764T (lt) | 2009-05-01 | 2020-06-10 | Promega Corporation | Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga |
| BR112013010487B1 (pt) * | 2010-11-02 | 2021-02-02 | Promega Corporation | compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro |
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