DK2990478T3 - Syntetiske oplophorus-luciferaser med forbedret lysemission - Google Patents
Syntetiske oplophorus-luciferaser med forbedret lysemission Download PDFInfo
- Publication number
- DK2990478T3 DK2990478T3 DK15186652.2T DK15186652T DK2990478T3 DK 2990478 T3 DK2990478 T3 DK 2990478T3 DK 15186652 T DK15186652 T DK 15186652T DK 2990478 T3 DK2990478 T3 DK 2990478T3
- Authority
- DK
- Denmark
- Prior art keywords
- gly
- val
- leu
- lys
- thr
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (19)
1. Polypeptid, der har mindst 60 % aminosyresekvensidentitet med en vildtype Oplophorus-\uc\ferase, og der omfatter mindst en aminosyresubstitution ved en position valgt fra positionerne 2, 4, 11, 20, 23, 28, 33, 34, 44, 45, 51, 54, 68, 72, 75, 76, 77, 89, 90, 92, 99, 104, 115, 124, 135, 138, 139, 143, 144, 164, 166, 167, eller 169 svarende til SEQ ID NO:l, hvor polypeptidet har mindst en af forbedret luminescens, forbedret signalstabilitet, og forbedret proteinstabilitet i forhold til vildtype Op/opftorus-luciferasen.
2. Polypeptidet ifølge krav 1, hvor polypeptidet har forbedret luminescens i forhold til vildtype Oplophorus-\uc\feraseu og en aminosyresubstitution ved én eller flere af positionerne 4, 11, 20, 28, 33, 54, 68, 75, 115, 124, 138, 143, eller 166 svarende til SEQ ID NO:l.
3. Polypeptidet ifølge et hvilket som helst af de foregående krav, hvor polypeptidet har forbedret proteinstabilitet i forhold til vildtype Oplophorus-luciferase, og en aminosyresubstitution ved én eller flere af positionerne 11, 20, 28, 33, 34, 44, 45, 51, 54, 68, 72, 75, 77, 89, 90, 92, 99, 104, 115, 124, 135, 138, 139, 143, 144, 164, 166, 167, eller 169 svarende til SEQ ID NO:l.
4. Polypeptidet ifølge et hvilket som helst af kravene 1-3, hvor polypeptidet yderligere omfatter en deletion af luciferaserester svarende til N-terminus af vildtype Op/opPorus-luciferasen.
5. Polypeptidet ifølge krav 4, hvor deletionen er 27 eller færre rester af N-terminus af vildtype Oplophorus-luciferasesekvensen.
6. Polypeptidet ifølge et hvilket som helst af kravene 1-5, hvor vildtype Oplophorus-\uc\ferasen er fra Oplophorus gracilirostris, Oplophorus grimaldii, Oplophorus spinicauda, Oplophorus foliaceus, Oplophorus novaezelandiae, Oplophorus typus, eller Oplophorus spinous.
7. Polypeptidet ifølge et hvilket som helst af kravene 1-6, hvor polypeptidet omfatter en substitution svarende til mindst én af T2S, A4E/S/R/G/D/T/L, Q11R/V/I/L/K/T, Q20R, E23V, S28P, A33K, L34M, V44I/L, V45E, G51V, A54F/T/V/G/S/W/L/I, F68S/Y/V, L72Q, M75R/K/Q/G/T/A, I76V, F77W, V79I, K89E, I90T/V, L92S/A/G/Q, I99V, P104L, P115E/I/Q/L/V/G/H/R/S/C/A/T, Q124K, N135K, Y138V/I/N/T/UC/R/M/K, D139E, I143L, N144K, C164S, N166R/K/A/L/P/Q/S, 1167V, eller 169L svarende til SEQ ID NO:l.
8. Polypeptidet ifølge et hvilket som helst af kravene 1-7, hvor polypeptidet omfatter aminosyresubstitutioner: (i) ved positionerne 11, 33, 44, 54, 115, 124, 138, og 166 svarende til SEQ ID NO: 1, (ii) ved positionerne 23, 28, 143, og 166 svarende til SEQ ID NO:l, (iii) ved positionerne 4, 34, 76, og 166 svarende til SEQ ID NO: 1, eller (iv) ved positionerne 51, 99, og 166 svarende til SEQ ID NO:l.
9. Polypeptidet ifølge krav 8, hvor polypeptidet yderligere omfatter aminosyresubstitutioner ved positionerne 45, 135, 104, 139, og 167 svarende til SEQ ID NO: 1.
10. Polypeptidet ifølge krav 8 eller 9, hvor polypeptidet yderligere omfatter aminosyresubstitutioner ved positionerne 28, 34, 51, 99 og 143 svarende til SEQ ID NO: 1.
11. Polypeptidet ifølge et af kravene 8-10, yderligere omfattende en substitution af mindst én aminosyre ved position 20, 54, 68, 72, 77, 79, 89, 90, 92, eller 164 svarende til SEQ ID NO:l.
12. Polypeptidet ifølge et hvilket som helst af kravene 1-11, hvor polypeptidet er et modificeret luciferasepolypeptid.
13. Fusionsprotein omfattende polypeptidet ifølge et af kravene 1-12 forbundet til et polypeptid af interesse.
14. Polynukleotid, der koder for fusionsproteinet ifølge krav 13.
15. Vektor omfattende polynukleotidet ifølge krav 14.
16. Vektoren ifølge krav 15, hvor polynukleotidet er operativt forbundet til en promoter.
17. Celle omfattende polypeptidet ifølge et af kravene 1-12, fusionsproteinet ifølge krav 13, polynukleotidet ifølge krav 14, eller vektoren ifølge krav 15 eller 16.
18. Ikke-humant transgent dyr omfattende polynukleotidet ifølge krav 14, vektoren ifølge krav 15 eller 16, eller cellen ifølge krav 17.
19. Kit omfattende polypeptidet ifølge et af kravene 1-12, fusionsproteinet ifølge krav 13, polynukleotidet ifølge krav 14, vektoren ifølge krav 15 eller 16, eller cellen ifølge krav 17.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17483809P | 2009-05-01 | 2009-05-01 | |
| EP10717013.6A EP2456864B1 (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with enhanced light output |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2990478T3 true DK2990478T3 (da) | 2017-05-15 |
Family
ID=42358981
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17154777.1T DK3181687T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forøget lysudbytte |
| DK10717013.6T DK2456864T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysemission |
| DK15186652.2T DK2990478T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysemission |
| DK18184699.9T DK3409764T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysudbytte |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17154777.1T DK3181687T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forøget lysudbytte |
| DK10717013.6T DK2456864T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysemission |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18184699.9T DK3409764T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysudbytte |
Country Status (16)
| Country | Link |
|---|---|
| US (7) | US8557970B2 (da) |
| EP (5) | EP3181687B1 (da) |
| JP (2) | JP6038649B2 (da) |
| KR (1) | KR101665352B1 (da) |
| CN (2) | CN108192950B (da) |
| AU (1) | AU2010242771B2 (da) |
| BR (1) | BRPI1010019B1 (da) |
| CA (1) | CA2758572A1 (da) |
| DK (4) | DK3181687T3 (da) |
| ES (5) | ES2795287T3 (da) |
| IL (3) | IL215697B (da) |
| LT (1) | LT3409764T (da) |
| PL (5) | PL2990478T3 (da) |
| PT (4) | PT2456864E (da) |
| SG (3) | SG10201601281WA (da) |
| WO (1) | WO2010127368A1 (da) |
Families Citing this family (76)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2795287T3 (es) | 2009-05-01 | 2020-11-23 | Promega Corp | Luciferasas de Oplophorus sintéticas con mayor emisión de luz |
| CN103443121B (zh) | 2010-11-02 | 2016-04-20 | 普罗梅加公司 | 新型腔肠素底物及其使用方法 |
| CA2815381C (en) | 2010-11-02 | 2019-03-19 | Promega Corporation | Coelenterazine derivatives and methods of using same |
| JP5896679B2 (ja) * | 2011-03-15 | 2016-03-30 | オリンパス株式会社 | オオオバボタル由来ルシフェラーゼ |
| WO2013078244A1 (en) | 2011-11-21 | 2013-05-30 | Promega Corporation | Carboxy x rhodamine analogs |
| US9482668B2 (en) | 2012-02-21 | 2016-11-01 | Laboratory Corporation Of America Holdings | Methods and systems for the detection of bacteria |
| US10519483B2 (en) | 2012-02-21 | 2019-12-31 | Laboratory Corporation Of America Holdings | Methods and systems for rapid detection of microorganisms using infectious agents |
| EP2900833B1 (en) | 2012-09-26 | 2018-05-30 | Promega Corporation | Real-time monitoring |
| US10067149B2 (en) | 2012-12-12 | 2018-09-04 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
| CN104853767B (zh) | 2012-12-12 | 2017-04-05 | 普洛麦格公司 | 用于捕获生物活性剂的细胞标靶的组合物和方法 |
| US10024862B2 (en) | 2012-12-12 | 2018-07-17 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
| JP6511720B2 (ja) * | 2013-02-28 | 2019-05-15 | Jnc株式会社 | コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法 |
| US10168323B2 (en) * | 2013-03-15 | 2019-01-01 | Promega Corporation | Compositions and methods for capture of cellular targets of bioactive agents |
| US11072812B2 (en) | 2013-03-15 | 2021-07-27 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
| AU2014236949C1 (en) | 2013-03-15 | 2018-11-29 | Promega Corporation | Activation of bioluminescence by structural complementation |
| CA2908266A1 (en) | 2013-03-27 | 2014-10-02 | Sample Technologies, Inc. | Recombinant phage and bacterial detection methods |
| JP6040846B2 (ja) | 2013-04-08 | 2016-12-07 | Jnc株式会社 | セレンテラジン類縁体 |
| AU2014281406A1 (en) | 2013-06-19 | 2016-01-21 | Sample6 Technologies, Inc. | Phage-based bacterial detection assay |
| JP6484987B2 (ja) | 2013-10-21 | 2019-03-20 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
| JP6442825B2 (ja) | 2013-12-26 | 2018-12-26 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
| US9790537B2 (en) | 2014-01-29 | 2017-10-17 | Promega Corporation | Quinone-masked probes as labeling reagents for cell uptake measurements |
| EP3099691B1 (en) | 2014-01-29 | 2019-11-20 | Promega Corporation | Pro-substrates for live cell applications |
| JP6256118B2 (ja) * | 2014-03-11 | 2018-01-10 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法 |
| JP6265020B2 (ja) * | 2014-04-16 | 2018-01-24 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
| US9732373B2 (en) | 2014-09-11 | 2017-08-15 | Promega Corporation | Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence |
| US11365402B2 (en) | 2014-09-12 | 2022-06-21 | Promega Corporation | Internal protein tags |
| EP4219736A1 (en) * | 2014-09-12 | 2023-08-02 | Promega Corporation | Internal protein tags |
| EP4455668A3 (en) | 2015-02-05 | 2025-02-19 | Promega Corporation | Luciferase-based thermal shift assays |
| JP6876002B2 (ja) | 2015-06-05 | 2021-05-26 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 機能的要素を共有結合により係留させるための細胞透過性、細胞適合性、かつ開裂可能であるリンカー |
| EP3313846B1 (en) | 2015-06-25 | 2020-05-06 | Promega Corporation | Thienopyrrole compounds and uses thereof as inhibitors of oplophorus-derived luciferases |
| US10316070B2 (en) | 2016-09-09 | 2019-06-11 | Promega Corporation | Dual protected pro-coelenterazine substrates |
| EP3516068B1 (en) | 2016-09-19 | 2025-04-23 | University of Southern California | Non-radioactive cytotoxicity assays |
| AU2017345560C1 (en) | 2016-10-20 | 2025-04-17 | President And Fellows Of Harvard College | In vitro and cell based assays for measuring the activity of botulinum neurotoxins |
| JP7200102B2 (ja) | 2016-11-01 | 2023-01-06 | プロメガ コーポレイション | エネルギー受容体に係留されたセレンテラジン類縁体 |
| EP3548492B1 (en) | 2016-12-01 | 2023-07-05 | Promega Corporation | Cell impermeable coelenterazine analogues |
| JP7161475B2 (ja) | 2016-12-28 | 2022-10-26 | プロメガ コーポレイション | 官能化ｎａｎｏｌｕｃ阻害剤 |
| EP3395803A1 (en) | 2017-04-28 | 2018-10-31 | Institut Pasteur | Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins |
| US11788068B2 (en) | 2017-10-25 | 2023-10-17 | Roche Molecular Systems, Inc. | Modified/mutant bacterial luciferases |
| US20200393446A1 (en) | 2017-12-08 | 2020-12-17 | Centre National De La Recherche Scientifique (Cnrs) | Bioluminescent screening test for detecting cell cytolysis |
| US10975363B2 (en) * | 2018-03-30 | 2021-04-13 | Inv Nylon Chemicals Americas, Llc | Materials and methods for biosynthetic manufacture and utilization of synthetic polypeptides, and products therefrom |
| US12338428B2 (en) | 2018-03-30 | 2025-06-24 | Inv Nylon Chemicals Americas, Llc | Materials and methods for managing aerobic gas fermentation |
| US12065636B2 (en) | 2018-03-30 | 2024-08-20 | Inv Nylon Chemicals Americas, Llc | High hydrogen utilization and gas recycle |
| EP3788048A1 (en) | 2018-05-01 | 2021-03-10 | Promega Corporation | Coelenterazine compounds as nanoluc suicide substrates |
| WO2019213033A1 (en) | 2018-05-02 | 2019-11-07 | Invista North America S.A.R.L. | Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-coa-tca balance in species of the genera ralstonia and cupriavidus and organisms related thereto |
| US11702680B2 (en) | 2018-05-02 | 2023-07-18 | Inv Nylon Chemicals Americas, Llc | Materials and methods for controlling PHA biosynthesis in PHA-generating species of the genera Ralstonia or Cupriavidus and organisms related thereto |
| WO2019232225A1 (en) | 2018-05-30 | 2019-12-05 | Promega Corporation | Broad-spectrum kinase binding agents |
| EP4140991B1 (en) | 2018-06-01 | 2024-05-15 | Promega Corporation | Inhibitors of oplophorus luciferase-derived bioluminescent complexes |
| EP3807419A4 (en) | 2018-06-12 | 2022-09-07 | Promega Corporation | MULTIPLE LUCIFERASE |
| US10962538B2 (en) | 2018-06-14 | 2021-03-30 | The Trustees Of Columbia University In The City Of New York | Assays using arrestin recruitment and unmodified receptors |
| IL318819A (en) | 2018-10-03 | 2025-04-01 | Promega Corp | Compounds and methods for stabilizing cholinetyrazine and its derivatives |
| WO2020113036A2 (en) | 2018-11-28 | 2020-06-04 | Promega Corporation | Reactive peptide labeling |
| EP3891503A2 (en) | 2018-12-04 | 2021-10-13 | Promega Corporation | Broad spectrum gpcr binding agents |
| US11913944B2 (en) | 2019-03-20 | 2024-02-27 | Promega Corporation | Photoaffinity probes |
| US11360096B2 (en) | 2019-05-16 | 2022-06-14 | Duke University | Complex BRET technique for measuring biological interactions |
| MX2021015900A (es) | 2019-06-21 | 2022-02-03 | Laboratory Corp America Holdings | Metodos para producir bacteriofagos mutantes para la deteccion de listeria. |
| CN114450326B (zh) | 2019-06-24 | 2025-02-11 | 普罗美加公司 | 用于将生物分子递送到细胞中的改性聚胺聚合物 |
| US11739363B2 (en) | 2019-08-26 | 2023-08-29 | Laboratory Corporation Of America Holdings | Devices and methods for detecting microorganisms using recombinant reproduction-deficient indicator bacteriophage |
| BR112022004479A2 (pt) | 2019-09-11 | 2022-05-31 | Laboratory Corp America Holdings | Métodos e sistemas para a rápida detecção de microrganismos usando agentes infecciosas recombinantes para expressar uma subunidade indicadora |
| IL293204B1 (en) | 2019-11-27 | 2026-08-01 | Promega Corp | Multipartite luciferase peptides and polypeptides |
| WO2021119149A1 (en) | 2019-12-10 | 2021-06-17 | Promega Corporation | Compositions and methods for bioluminescent detection using multifunctional probes |
| EP4096691A1 (en) | 2020-01-28 | 2022-12-07 | Freeline Therapeutics Limited | Improved assay for determining neutralising antibody titre to a viral vektor |
| JP7677997B2 (ja) | 2020-05-22 | 2025-05-15 | プロメガ コーポレイション | キナーゼターゲットエンゲージメントの増強 |
| WO2022047186A1 (en) | 2020-08-28 | 2022-03-03 | Promega Corporation | Target engagement assay for ras proteins |
| TW202242415A (zh) | 2020-12-25 | 2022-11-01 | 日商中外製藥股份有限公司 | 能夠與複數的目標分子共同形成複合體之候補分子的篩選方法 |
| CN115161296B (zh) * | 2021-04-01 | 2024-08-13 | 深圳华大生命科学研究院 | 一种刺虾荧光素酶Nluc的突变体及其应用 |
| CN113025586B (zh) * | 2021-04-06 | 2022-10-04 | 湖北省中医院 | 一种经改造的萤光素酶突变体蛋白、生物发光探针、探针组、制备方法和检测方法 |
| EP4337646A4 (en) | 2021-05-13 | 2025-05-21 | Promega Corporation | BIOLUMINESCENT DETECTION OF DNA SYNTHESIS |
| JP2025516354A (ja) | 2022-05-04 | 2025-05-27 | プロメガ コーポレイション | スプリット修飾デハロゲナーゼバリアント |
| US20240132859A1 (en) | 2022-05-04 | 2024-04-25 | Promega Corporation | Modified dehalogenase with extended surface loop regions |
| WO2023215514A2 (en) | 2022-05-04 | 2023-11-09 | Promega Corporation | Bioluminescence-triggered photocatalytic labeling |
| EP4519290A1 (en) | 2022-05-04 | 2025-03-12 | Promega Corporation | Circularly permuted dehalogenase variants |
| GB202213479D0 (en) | 2022-09-14 | 2022-10-26 | Ipsen Biopharm Ltd | Cell-free clostridial neurotoxin assays |
| US20260077065A1 (en) * | 2022-09-15 | 2026-03-19 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and Methods for Assessing Kinase Activity |
| GB202404021D0 (en) | 2024-03-20 | 2024-05-01 | Ipsen Biopharm Ltd | Cell-based neurotoxin assay |
| CN118516381B (zh) * | 2024-07-22 | 2024-11-01 | 北京剂泰医药科技有限公司 | 编码荧光素酶的mRNA及其应用 |
| CN119709661B (zh) * | 2024-12-30 | 2025-10-10 | 佰翱得(无锡)新药开发有限公司 | 一种荧光素酶OLuc突变体及其应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8911957D0 (en) | 1989-05-24 | 1989-07-12 | Interox Chemicals Ltd | Peroxyacid manufacture |
| US5368484A (en) | 1992-05-22 | 1994-11-29 | Atari Games Corp. | Vehicle simulator with realistic operating feedback |
| ES2301160T3 (es) * | 1994-01-03 | 2008-06-16 | Promega Corporation | Luciferasas mutantes. |
| EP2860252A1 (en) * | 1997-09-19 | 2015-04-15 | Promega Corporation | Thermostable luciferases and methods of production |
| JP3694181B2 (ja) * | 1999-01-05 | 2005-09-14 | キッコーマン株式会社 | 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法 |
| RU2251571C2 (ru) | 1999-10-26 | 2005-05-10 | Дзе Секретэри Оф Стейт Фор Дефенс | РЕКОМБИНАНТНАЯ ТЕРМОСТАБИЛЬНАЯ ЛЮЦИФЕРАЗА, СПОСОБ ЕЕ ПОЛУЧЕНИЯ, ИЗОЛИРОВАННАЯ НУКЛЕИНОВАЯ КИСЛОТА, ЭКСПРЕССИРУЮЩИЙ ВЕКТОР, НАБОР ДЛЯ ИСПОЛЬЗОВАНИЯ В БИОЛЮМИНЕСЦЕНТНОМ АНАЛИЗЕ, АНАЛИТИЧЕСКИЙ ТЕСТ ДЛЯ ОПРЕДЕЛЕНИЯ ПРИСУТСТВИЯ В ОБРАЗЦЕ СоА |
| EP1156103B1 (en) * | 2000-04-26 | 2010-08-11 | Chisso Corporation | Oplophorus luciferase |
| JP4613441B2 (ja) * | 2000-04-26 | 2011-01-19 | チッソ株式会社 | 新規ルシフェラーゼおよび発光蛋白質 |
| US7118878B1 (en) | 2000-06-09 | 2006-10-10 | Promega Corporation | Method for increasing luminescence assay sensitivity |
| US7268229B2 (en) | 2001-11-02 | 2007-09-11 | Promega Corporation | Compounds to co-localize luminophores with luminescent proteins |
| AU2003251594A1 (en) | 2002-06-19 | 2004-01-06 | Medical Instill Technologies, Inc. | Sterile filling machine having needle filling station within e-beam chamber |
| CA2561809A1 (en) * | 2004-03-31 | 2005-10-20 | Xencor, Inc. | Bmp-7 variants with improved properties |
| JP3844082B2 (ja) * | 2005-04-08 | 2006-11-08 | キッコーマン株式会社 | 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び耐熱性ホタルルシフェラーゼの製造法 |
| JP4999341B2 (ja) * | 2005-09-06 | 2012-08-15 | 株式会社バイオエネックス | 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法 |
| US9359635B2 (en) * | 2006-04-03 | 2016-06-07 | Promega Corporation | Permuted and nonpermuted luciferase biosensors |
| US20080248511A1 (en) * | 2007-03-26 | 2008-10-09 | Promega Corporation | Methods to quench light from optical reactions |
| CN101849005A (zh) * | 2007-11-05 | 2010-09-29 | 普罗梅加公司 | 杂合融合报道子及其应用 |
| ATE542903T1 (de) * | 2008-05-19 | 2012-02-15 | Promega Corp | Luciferase-biosensoren für camp |
| WO2010119721A1 (ja) | 2009-04-17 | 2010-10-21 | 独立行政法人産業技術総合研究所 | 超高輝度で安定な人工生物発光酵素 |
| ES2795287T3 (es) | 2009-05-01 | 2020-11-23 | Promega Corp | Luciferasas de Oplophorus sintéticas con mayor emisión de luz |
| CN103443121B (zh) * | 2010-11-02 | 2016-04-20 | 普罗梅加公司 | 新型腔肠素底物及其使用方法 |
-
2010
- 2010-05-03 ES ES18184699T patent/ES2795287T3/es active Active
- 2010-05-03 US US12/773,002 patent/US8557970B2/en active Active
- 2010-05-03 PT PT107170136T patent/PT2456864E/pt unknown
- 2010-05-03 PL PL15186652T patent/PL2990478T3/pl unknown
- 2010-05-03 JP JP2012508822A patent/JP6038649B2/ja active Active
- 2010-05-03 LT LTEP18184699.9T patent/LT3409764T/lt unknown
- 2010-05-03 DK DK17154777.1T patent/DK3181687T3/da active
- 2010-05-03 EP EP17154777.1A patent/EP3181687B1/en active Active
- 2010-05-03 DK DK10717013.6T patent/DK2456864T3/da active
- 2010-05-03 PT PT181846999T patent/PT3409764T/pt unknown
- 2010-05-03 SG SG10201601281WA patent/SG10201601281WA/en unknown
- 2010-05-03 WO PCT/US2010/033449 patent/WO2010127368A1/en not_active Ceased
- 2010-05-03 SG SG2011074242A patent/SG175180A1/en unknown
- 2010-05-03 PL PL18184699T patent/PL3409764T3/pl unknown
- 2010-05-03 PL PL10717013T patent/PL2456864T3/pl unknown
- 2010-05-03 EP EP20169057.5A patent/EP3744834B1/en active Active
- 2010-05-03 PL PL17154777T patent/PL3181687T3/pl unknown
- 2010-05-03 CN CN201810043641.3A patent/CN108192950B/zh active Active
- 2010-05-03 CA CA2758572A patent/CA2758572A1/en active Pending
- 2010-05-03 SG SG10201901453PA patent/SG10201901453PA/en unknown
- 2010-05-03 ES ES20169057T patent/ES3042791T3/es active Active
- 2010-05-03 PT PT17154777T patent/PT3181687T/pt unknown
- 2010-05-03 EP EP15186652.2A patent/EP2990478B1/en active Active
- 2010-05-03 PT PT151866522T patent/PT2990478T/pt unknown
- 2010-05-03 DK DK15186652.2T patent/DK2990478T3/da active
- 2010-05-03 ES ES17154777T patent/ES2691539T3/es active Active
- 2010-05-03 BR BRPI1010019A patent/BRPI1010019B1/pt active IP Right Grant
- 2010-05-03 AU AU2010242771A patent/AU2010242771B2/en active Active
- 2010-05-03 ES ES10717013.6T patent/ES2559505T3/es active Active
- 2010-05-03 CN CN201080019477.4A patent/CN102459579B/zh active Active
- 2010-05-03 EP EP18184699.9A patent/EP3409764B1/en active Active
- 2010-05-03 EP EP10717013.6A patent/EP2456864B1/en active Active
- 2010-05-03 ES ES15186652.2T patent/ES2629390T3/es active Active
- 2010-05-03 PL PL20169057.5T patent/PL3744834T3/pl unknown
- 2010-05-03 KR KR1020117028749A patent/KR101665352B1/ko active Active
- 2010-05-03 DK DK18184699.9T patent/DK3409764T3/da active
-
2011
- 2011-10-11 IL IL215697A patent/IL215697B/en active IP Right Grant
-
2013
- 2013-10-14 US US14/053,252 patent/US9777311B2/en active Active
-
2015
- 2015-10-22 JP JP2015208321A patent/JP6227615B2/ja active Active
-
2017
- 2017-09-25 US US15/714,210 patent/US10233485B2/en active Active
-
2019
- 2019-03-06 US US16/294,464 patent/US10633690B2/en active Active
- 2019-03-10 IL IL265263A patent/IL265263B/en active IP Right Grant
-
2020
- 2020-03-17 US US16/821,682 patent/US10844422B2/en active Active
- 2020-04-22 IL IL274145A patent/IL274145B/en unknown
- 2020-10-20 US US17/075,415 patent/US11365436B2/en active Active
-
2022
- 2022-06-20 US US17/844,371 patent/US11667950B2/en active Active
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11365436B2 (en) | Synthetic oplophorus luciferases with enhanced light output | |
| CN109312328B (zh) | 突变型甲虫荧光素酶、基因、重组载体、转化体，以及突变型甲虫荧光素酶的制备方法 | |
| KR20180105772A (ko) | 생물발광 강도가 증폭된 변이 가우시아 루시퍼라아제 |