DK2660333T4 - Nicking og udvidelsesamplificeringsreaktion til exponentiel amplificering af nukleinsyrer - Google Patents
Nicking og udvidelsesamplificeringsreaktion til exponentiel amplificering af nukleinsyrer Download PDFInfo
- Publication number
- DK2660333T4 DK2660333T4 DK12195333.5T DK12195333T DK2660333T4 DK 2660333 T4 DK2660333 T4 DK 2660333T4 DK 12195333 T DK12195333 T DK 12195333T DK 2660333 T4 DK2660333 T4 DK 2660333T4
- Authority
- DK
- Denmark
- Prior art keywords
- nicking
- nucleic acids
- amplification
- extension
- exponential
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title 2
- 238000003199 nucleic acid amplification method Methods 0.000 title 2
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/778,018 US9689031B2 (en) | 2007-07-14 | 2007-07-14 | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| EP08781827.4A EP2181196B1 (en) | 2007-07-14 | 2008-07-14 | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK2660333T3 DK2660333T3 (da) | 2019-12-16 |
| DK2660333T4 true DK2660333T4 (da) | 2024-05-06 |
Family
ID=40175258
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12195331.9T DK2657350T3 (da) | 2007-07-14 | 2008-07-14 | Nicking- og forlængelsesamplifikationsreaktion til eksponentiel amplifikation af nucleinsyrer |
| DK08781827.4T DK2181196T3 (da) | 2007-07-14 | 2008-07-14 | Nicking- og forlængelsesamplifikationsreaktioner til eksponentiel amplificering af nucleinsyrer |
| DK12195333.5T DK2660333T4 (da) | 2007-07-14 | 2008-07-14 | Nicking og udvidelsesamplificeringsreaktion til exponentiel amplificering af nukleinsyrer |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12195331.9T DK2657350T3 (da) | 2007-07-14 | 2008-07-14 | Nicking- og forlængelsesamplifikationsreaktion til eksponentiel amplifikation af nucleinsyrer |
| DK08781827.4T DK2181196T3 (da) | 2007-07-14 | 2008-07-14 | Nicking- og forlængelsesamplifikationsreaktioner til eksponentiel amplificering af nucleinsyrer |
Country Status (13)
| Country | Link |
|---|---|
| US (9) | US9689031B2 (da) |
| EP (4) | EP3754029A1 (da) |
| JP (2) | JP5693955B2 (da) |
| CN (4) | CN106544409A (da) |
| AU (5) | AU2008276118B2 (da) |
| BR (1) | BRPI0814527B1 (da) |
| CA (2) | CA2693805C (da) |
| DK (3) | DK2657350T3 (da) |
| ES (3) | ES2816748T3 (da) |
| FI (1) | FI2660333T4 (da) |
| PL (1) | PL2657350T3 (da) |
| PT (1) | PT2657350T (da) |
| WO (1) | WO2009012246A2 (da) |
Families Citing this family (130)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| DE60322044D1 (de) | 2002-02-21 | 2008-08-21 | Asm Scient Inc | Rekombinase-polymerase-amplifikation |
| JP5027126B2 (ja) | 2005-07-25 | 2012-09-19 | アリーア サン ディエゴ， インコーポレイテッド | リコンビナーゼポリメラーゼ増幅を多重化するための方法 |
| US8071308B2 (en) | 2006-05-04 | 2011-12-06 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| EP2419538B1 (en) * | 2009-04-16 | 2016-09-14 | Padma Arunachalam | Methods and compositions to detect and differentiate small rnas in rna maturation pathway |
| JP5847076B2 (ja) | 2009-05-20 | 2016-01-20 | バイオサイト インコーポレイテッド | Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質 |
| ES3022638T3 (en) | 2009-06-05 | 2025-05-28 | Abbott Diagnostics Scarborough Inc | Recombinase polymerase amplification reagents |
| EP2443254A2 (en) | 2009-06-15 | 2012-04-25 | NetBio, Inc. | Improved methods for forensic dna quantitation |
| US20110003703A1 (en) * | 2009-07-01 | 2011-01-06 | Charles Ma | Nucleic Acid Hybridization and Detection Using Enzymatic Reactions on a Microarray |
| EP2287334A1 (de) * | 2009-08-21 | 2011-02-23 | Qiagen GmbH | Verfahren zum Nachweis von Zielnukleinsäuren |
| JP2013523097A (ja) * | 2010-03-30 | 2013-06-17 | モノクアント・ピーティーワイ・リミテッド | オリゴヌクレオチド機能性を制御する方法 |
| US10232374B2 (en) | 2010-05-05 | 2019-03-19 | Miroculus Inc. | Method of processing dried samples using digital microfluidic device |
| EP2418286A1 (en) | 2010-08-10 | 2012-02-15 | QIAGEN GmbH | Improved method for isothermal amplification of nucleic acids |
| US12043866B2 (en) | 2010-08-13 | 2024-07-23 | Envirologix Inc. | Compositions and methods for quantifying a nucleic acid sequence in a sample |
| EP2420579A1 (en) * | 2010-08-17 | 2012-02-22 | QIAGEN GmbH | Helicase dependent isothermal amplification using nicking enzymes |
| US9040243B2 (en) | 2010-09-23 | 2015-05-26 | Qiagen Gmbh | Method for detecting and/or quantifying human DNA |
| JPWO2012070618A1 (ja) | 2010-11-24 | 2014-05-19 | 株式会社カネカ | 増幅核酸検出方法及び検出デバイス |
| JP6126381B2 (ja) | 2010-12-10 | 2017-05-10 | 国立大学法人東京工業大学 | 標的核酸の検出方法及びキット |
| WO2012113780A1 (en) | 2011-02-21 | 2012-08-30 | Qiagen Gmbh | Method for quantifying human dna |
| EP2678443B1 (en) | 2011-02-21 | 2016-11-23 | Qiagen GmbH | Method for quantifying human dna using an internal control |
| SG10201605049QA (en) * | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
| US9352312B2 (en) | 2011-09-23 | 2016-05-31 | Alere Switzerland Gmbh | System and apparatus for reactions |
| US9617590B2 (en) | 2011-09-30 | 2017-04-11 | Stc.Unm | DNA sample preparation and sequencing |
| KR101358416B1 (ko) * | 2012-02-16 | 2014-02-11 | 한국과학기술원 | 리가제 반응과 절단효소 증폭반응을 이용한 표적 유전자 또는 이의 돌연변이 검출방법 |
| UA116205C2 (uk) | 2012-04-09 | 2018-02-26 | Енвіролоджикс Інк. | Спосіб кількісного визначення послідовності нуклеїнової кислоти в зразку |
| CN114214394A (zh) * | 2012-06-08 | 2022-03-22 | 爱奥尼安技术公司 | 核苷酸扩增反应 |
| CN103667252A (zh) * | 2012-09-10 | 2014-03-26 | 思洛生物技术股份有限公司 | 一种核酸扩增方法 |
| CN103173537B (zh) * | 2012-12-21 | 2014-09-10 | 山东出入境检验检疫局检验检疫技术中心 | 大肠埃希氏菌o157:h7的切刻内切酶核酸恒温扩增快速检测试剂盒 |
| US12385089B2 (en) * | 2013-02-05 | 2025-08-12 | Bionano Genomics, Inc. | Methods for single-molecule analysis |
| US10590474B2 (en) | 2013-03-11 | 2020-03-17 | Elitechgroup B.V. | Methods for true isothermal strand displacement amplification |
| WO2014164479A1 (en) * | 2013-03-11 | 2014-10-09 | Elitech Holding B.V. | Methods for true isothermal strand displacement amplification |
| EP3058100A4 (en) * | 2013-10-18 | 2017-04-19 | California Institute of Technology | Enhanced nucleic acid identification and detection |
| US10392652B2 (en) | 2013-11-22 | 2019-08-27 | Kaneka Corporation | Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end |
| JP6739339B2 (ja) | 2014-01-15 | 2020-08-12 | アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ | カバーされた配列変換ｄｎａおよび検出方法 |
| ES2719122T3 (es) | 2014-03-21 | 2019-07-08 | Life Technologies Corp | Ensayo de referencia multi-copia |
| EP3748011B1 (en) | 2014-04-22 | 2024-11-13 | Envirologix Inc. | Compositions and methods for enhancing and/or predicting dna amplification |
| WO2015168134A1 (en) | 2014-04-30 | 2015-11-05 | Envirologix, Inc. | Compositions and methods for detecting huanglongbing |
| WO2016059474A2 (en) | 2014-10-14 | 2016-04-21 | Abbott Japan Co., Ltd | Sequence conversion and signal amplifier dna having poly dna spacer sequences and detection methods using same |
| BR112017008082A2 (pt) * | 2014-10-20 | 2017-12-26 | Envirologix Inc | composições e métodos para detectar um vírus de rna |
| EP3237641B1 (en) | 2014-12-24 | 2020-04-08 | Abbott Laboratories | Detection method, composition and kit based on signal amplifier dna cascade reactions with target extension |
| CN105154556B (zh) * | 2015-01-23 | 2018-10-30 | 中国人民解放军第三军医大学第一附属医院 | 实时荧光恒温指数扩增方法 |
| EP3250715B1 (en) | 2015-01-30 | 2024-10-23 | Envirologix Inc. | A substrate for a nicking and extension reaction comprising a nucleic acid duplex |
| AU2016252998B2 (en) | 2015-04-24 | 2021-11-04 | Atila Biosystems Incorporated | Amplification with primers of limited nucleotide composition |
| US10695762B2 (en) | 2015-06-05 | 2020-06-30 | Miroculus Inc. | Evaporation management in digital microfluidic devices |
| CN108026494A (zh) | 2015-06-05 | 2018-05-11 | 米罗库鲁斯公司 | 限制蒸发和表面结垢的空气基质数字微流控装置和方法 |
| CN104988235B (zh) * | 2015-07-20 | 2019-01-22 | 北京福德安科技有限公司 | 一种切克酶介导等温扩增小分子信标定量检测技术 |
| CN105112507A (zh) * | 2015-07-30 | 2015-12-02 | 中国人民解放军第三军医大学第一附属医院 | 微小rna的数字化恒温检测方法 |
| CN105063190A (zh) * | 2015-07-30 | 2015-11-18 | 中国人民解放军第三军医大学第一附属医院 | 微小rna的固相芯片恒温检测方法 |
| CN105154534A (zh) * | 2015-07-30 | 2015-12-16 | 中国人民解放军第三军医大学第一附属医院 | 微小rna的实时恒温指数扩增方法 |
| EP3978632A1 (en) | 2015-09-15 | 2022-04-06 | Neogen Corporation | Means for detecting listeria from an environmental sample |
| GB201519565D0 (en) | 2015-11-05 | 2015-12-23 | Alere San Diego Inc | Sample preparation device |
| EP3368654B1 (en) | 2015-10-30 | 2025-11-19 | Abbott Diagnostics Scarborough, Inc. | Determination of polymorphisms using isothermal nucleic acid amplification |
| US10329601B2 (en) * | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
| WO2017141067A1 (en) | 2016-02-16 | 2017-08-24 | The University Of Tokyo | Method of eliminating background amplification of nucleic acid targets |
| ES3032616T3 (en) | 2016-02-26 | 2025-07-22 | Abbott Diagnostics Scarborough Inc | Redox labeled oligonucleotide probes and their use |
| CA3016582A1 (en) | 2016-03-04 | 2017-09-08 | Alere San Diego, Inc. | Automated nested recombinase polymerase amplification |
| US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US20190176153A1 (en) | 2016-05-18 | 2019-06-13 | Roche Sequencing Solutions, Inc. | Quantitative real time pcr amplification using an electrowetting-based device |
| US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
| KR101820440B1 (ko) * | 2016-06-28 | 2018-01-22 | 한국과학기술원 | 표적핵산의 검출방법 |
| GB201611469D0 (en) * | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| CN109715781A (zh) | 2016-08-22 | 2019-05-03 | 米罗库鲁斯公司 | 用于数字微流控设备中的并行液滴控制的反馈系统 |
| US11198910B2 (en) * | 2016-09-02 | 2021-12-14 | New England Biolabs, Inc. | Analysis of chromatin using a nicking enzyme |
| CN109715646B (zh) | 2016-09-15 | 2023-02-10 | 雅培实验室 | 用于样品分析的装置和方法 |
| US11078527B2 (en) | 2016-09-16 | 2021-08-03 | Qiagen Gmbh | Method for quantifying and/or detecting human male DNA |
| EP3512962B1 (en) | 2016-09-16 | 2026-02-11 | QIAGEN GmbH | Method and kit for determining nucleic acid degradation |
| JP7306696B2 (ja) | 2016-09-30 | 2023-07-11 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Ｒｎａ誘導型核酸修飾酵素及びその使用方法 |
| CA3038960A1 (en) | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
| CN110383061A (zh) | 2016-12-28 | 2019-10-25 | 米罗库鲁斯公司 | 数字微流控设备和方法 |
| GB201701262D0 (en) | 2017-01-25 | 2017-03-08 | Sense Biodetection Ltd | Nucleic acid detection method |
| CA3054610A1 (en) | 2017-02-28 | 2018-09-07 | Alere San Diego, Inc. | Microfluidic devices and related methods |
| US20200017862A1 (en) * | 2017-03-07 | 2020-01-16 | University Of North Carolina Charlotte | Systems and methods for single-strand break signaling and repair in a cell-free system |
| US11623219B2 (en) | 2017-04-04 | 2023-04-11 | Miroculus Inc. | Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets |
| US20200048691A1 (en) * | 2017-04-17 | 2020-02-13 | Montana State University | Switch-like isothermal dna amplification demonstrating a non-linear amplification rate |
| EP3658908B1 (en) | 2017-07-24 | 2025-11-12 | Integra Biosciences AG | Digital microfluidics systems and methods with integrated plasma collection device |
| CN111406118B (zh) * | 2017-08-31 | 2024-07-12 | 爱奥尼安技术有限责任公司 | 呼吸道合胞病毒种属的切口产生和延伸扩增反应(near) |
| CA3073058A1 (en) | 2017-09-01 | 2019-03-07 | Miroculus Inc. | Digital microfluidics devices and methods of using them |
| WO2019089804A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Casy compositions and methods of use |
| JP2021503278A (ja) | 2017-11-01 | 2021-02-12 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | ＣａｓＺ組成物及び使用方法 |
| WO2019089808A1 (en) * | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Class 2 crispr/cas compositions and methods of use |
| GB2569965A (en) * | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| EA202091673A1 (ru) | 2018-01-29 | 2021-02-03 | Ст. Джуд Чилдрен'С Рисерч Хоспитал, Инк. | Способ амплификации нуклеиновых кислот |
| GB201804585D0 (en) * | 2018-03-22 | 2018-05-09 | Dnae Diagnostics Ltd | Methods for amplication of nucleic acids with Endonuclease-Mediated shifting equilibrium amplification (EM-SEq) |
| WO2019226919A1 (en) | 2018-05-23 | 2019-11-28 | Miroculus Inc. | Control of evaporation in digital microfluidics |
| MX2020013461A (es) * | 2018-06-26 | 2021-04-28 | Broad Inst Inc | Composiciones, sistemas y métodos de amplificación basados en la doble nicasa crispr. |
| CN109055524B (zh) * | 2018-07-24 | 2022-03-15 | 中山大学 | 一种基于DNA组装的恒温条件下同时检测多种microRNA的探针和方法 |
| GB201812149D0 (en) | 2018-07-25 | 2018-09-05 | Sense Biodetection Ltd | Nucleic acid detection method |
| EP3830301B1 (en) | 2018-08-01 | 2024-05-22 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
| CN109136353B (zh) * | 2018-08-21 | 2021-11-16 | 中山大学 | 一种低序列依赖高阶恒温指数扩增检测microRNA的方法 |
| EP3856924A4 (en) * | 2018-09-26 | 2022-06-22 | Lamprogen, Inc. | DIGITAL AMPLIFICATION FOR PROTEIN DETECTION |
| CN109321669B (zh) * | 2018-10-29 | 2022-03-15 | 江南大学 | 一种基于嵌合体序列设计和分子信标的荧光检测金黄色葡萄球菌的方法 |
| CN109593863A (zh) * | 2018-11-28 | 2019-04-09 | 江南大学 | 一种链置换型dna聚合酶诱导等温循环扩增反应检测肉类来源的方法 |
| EP3931313A2 (en) | 2019-01-04 | 2022-01-05 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
| EP3917670A4 (en) | 2019-01-31 | 2022-11-02 | Miroculus Inc. | ANTIFOULING COMPOSITIONS AND METHODS FOR HANDLING AND TREATING ENCAPSULATED DROPLET |
| US12582991B2 (en) | 2019-02-28 | 2026-03-24 | Integra Biosciences Ag | Digital microfluidics devices and methods of using them |
| CN114206499B (zh) | 2019-04-08 | 2024-10-08 | 米罗库鲁斯公司 | 多盒式数字微流控装置和使用方法 |
| GB201915346D0 (en) | 2019-06-21 | 2019-12-04 | Lumiradx Tech Ltd | Improvements in or relating to nicking enzymes |
| KR102531590B1 (ko) * | 2019-07-16 | 2023-05-11 | 고려대학교 산학협력단 | 절단효소 인식부위의 미러링 결합에 의한 표적핵산의 검출 방법 및 핵산 검출용 조성물 |
| JP2021019581A (ja) * | 2019-07-25 | 2021-02-18 | 株式会社リコー | 標準核酸試料調製用鋳型核酸分子 |
| US11524298B2 (en) | 2019-07-25 | 2022-12-13 | Miroculus Inc. | Digital microfluidics devices and methods of use thereof |
| CA3149610A1 (en) | 2019-07-31 | 2021-02-04 | BioSkryb Genomics, Inc. | Single cell analysis |
| CA3167895A1 (en) | 2020-01-25 | 2021-07-29 | Sense Biodetection Limited | Virus detection |
| WO2021162642A1 (en) * | 2020-02-15 | 2021-08-19 | Agency For Science, Technology And Research | A method to amplify a nucleic acid |
| CN111979303A (zh) * | 2020-08-11 | 2020-11-24 | 上海奕谱生物科技有限公司 | 一种核酸检测试剂盒、方法及其应用 |
| CN116670297A (zh) | 2020-10-22 | 2023-08-29 | 纽勤有限公司 | 从环境样品中检测李斯特菌的方法 |
| CN112899349B (zh) * | 2020-12-03 | 2022-12-27 | 青岛大学附属医院 | 同时检测一种或多种靶标核酸的可视化核酸检测方法及其应用 |
| CN112646862B (zh) * | 2020-12-28 | 2022-07-15 | 天津市疾病预防控制中心 | 一种新型的自我链置换原理及其多重链置换反应的应用 |
| CN112760362B (zh) * | 2021-02-04 | 2023-07-07 | 中国人民解放军陆军特色医学中心 | 一种用于寡核苷酸扩增的环形信号扩增模板及其应用 |
| JP2024518331A (ja) | 2021-04-29 | 2024-05-01 | アボット・ラボラトリーズ | 生体試料のハイスループット核酸検査 |
| EP4347868A1 (en) | 2021-05-27 | 2024-04-10 | Lumiradx Uk Ltd | Improvements in or relating to digestion of reaction products |
| WO2022261281A1 (en) * | 2021-06-09 | 2022-12-15 | New England Biolabs, Inc. | Target initiation and amplification of long dna with nuclease and replisome enzymes |
| CN113481283A (zh) * | 2021-06-30 | 2021-10-08 | 上海伯杰医疗科技有限公司北京分公司 | 等温扩增核酸靶序列的方法 |
| US20240271186A1 (en) | 2021-08-03 | 2024-08-15 | Wageningen Universiteit | Argonaute-based nucleic acid detection system |
| US11772093B2 (en) | 2022-01-12 | 2023-10-03 | Miroculus Inc. | Methods of mechanical microfluidic manipulation |
| WO2024030985A1 (en) | 2022-08-04 | 2024-02-08 | Abbott Laboratories | Assays for detecting monkeypox virus |
| EP4573553A1 (en) | 2022-08-15 | 2025-06-25 | ABSCI Corporation | Quantitative affinity activity specific cell enrichment |
| WO2025030178A1 (en) | 2023-08-03 | 2025-02-06 | Abbott Laboratories | Isothermal amplification of nucleic acids |
| WO2025049450A1 (en) | 2023-08-28 | 2025-03-06 | Abbott Molecular, Inc. | Assays for detecting bk virus (bkv) |
| WO2025049451A1 (en) | 2023-08-28 | 2025-03-06 | Abbott Molecular, Inc. | Assays for detecting cytomegalovirus (cmv) or epstein-bar virus (ebv) |
| WO2025050131A1 (en) | 2023-09-01 | 2025-03-06 | Abbott Laboratories | Methods of detecting variant nucleic acids |
| WO2025111147A1 (en) | 2023-11-21 | 2025-05-30 | Abbott Laboratories | Two-dimensional matrix droplet array |
| WO2025129195A1 (en) | 2023-12-15 | 2025-06-19 | New England Biolabs, Inc. | Dna polymerases and related methods |
| WO2025137528A1 (en) | 2023-12-20 | 2025-06-26 | Abbott Laboratories | Reducing agent compositions for isothermal amplification of nucleic acids |
| WO2025144700A1 (en) | 2023-12-27 | 2025-07-03 | Absci Corporation | Nanobody library screening using bacterial surface display |
| WO2025171104A1 (en) * | 2024-02-08 | 2025-08-14 | Alere San Diego, Inc. | Universal hairpin probes |
| WO2025240630A1 (en) | 2024-05-14 | 2025-11-20 | Abbott Laboratories | Reagent compositions for isothermal amplification of nucleic acids |
| US20260092310A1 (en) | 2024-09-27 | 2026-04-02 | New England Biolabs, Inc. | Cas12a compositions and methods |
Family Cites Families (116)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6040166A (en) | 1985-03-28 | 2000-03-21 | Roche Molecular Systems, Inc. | Kits for amplifying and detecting nucleic acid sequences, including a probe |
| US5273879A (en) * | 1987-07-23 | 1993-12-28 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5455166A (en) * | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| US5556751A (en) * | 1991-04-25 | 1996-09-17 | Amoco Corporation | Selective amplification system using Q-β replicase |
| US5846717A (en) * | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
| FR2683827B1 (fr) * | 1991-11-15 | 1994-03-04 | Institut Nal Sante Recherc Medic | Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique. |
| US5270184A (en) * | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
| WO1994003624A1 (en) * | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US5834202A (en) * | 1992-08-04 | 1998-11-10 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| WO1994003635A1 (en) | 1992-08-04 | 1994-02-17 | Institute Of Molecular Biology & Biotechnology | Rapid amplification and detection of nucleic acids |
| US5614389A (en) * | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5733733A (en) * | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5470723A (en) | 1993-05-05 | 1995-11-28 | Becton, Dickinson And Company | Detection of mycobacteria by multiplex nucleic acid amplification |
| US5422252A (en) * | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
| US5457027A (en) | 1993-05-05 | 1995-10-10 | Becton, Dickinson And Company | Internal controls for isothermal nucleic acid amplification reactions |
| WO1995025180A1 (en) * | 1994-03-16 | 1995-09-21 | Gen-Probe Incorporated | Isothermal strand displacement nucleic acid amplification |
| US5547861A (en) * | 1994-04-18 | 1996-08-20 | Becton, Dickinson And Company | Detection of nucleic acid amplification |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| US5942391A (en) * | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
| WO1996020287A2 (en) * | 1994-12-23 | 1996-07-04 | Behringwerke Aktiengesellschaft | Detection of nucleic acids by target-catalyzed product formation |
| US5556771A (en) | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
| AUPN245295A0 (en) * | 1995-04-13 | 1995-05-11 | Johnson & Johnson Research Pty. Limited | Assay for genetic abnormalities |
| AT402203B (de) * | 1995-06-13 | 1997-03-25 | Himmler Gottfried Dipl Ing Dr | Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren |
| US5681705A (en) * | 1995-08-28 | 1997-10-28 | Schram; James L. | Amplification and detection of mycobacterium avium complex species |
| US5916779A (en) * | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
| US5747255A (en) * | 1995-09-29 | 1998-05-05 | Lynx Therapeutics, Inc. | Polynucleotide detection by isothermal amplification using cleavable oligonucleotides |
| US5985557A (en) * | 1996-01-24 | 1999-11-16 | Third Wave Technologies, Inc. | Invasive cleavage of nucleic acids |
| US7432048B2 (en) * | 1996-11-29 | 2008-10-07 | Third Wave Technologies, Inc. | Reactions on a solid surface |
| JP2000505312A (ja) * | 1996-03-18 | 2000-05-09 | モレキュラー バイオロジー リソーシーズ，インコーポレイテッド | 標的核酸配列増幅 |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| WO1998002582A2 (en) * | 1996-07-16 | 1998-01-22 | Gen-Probe Incorporated | Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific t¿m? |
| US5853990A (en) * | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| AU712873B2 (en) | 1996-08-29 | 1999-11-18 | Tapestry Pharmaceuticals, Inc. | Methods for targeting, enriching, detecting and/or isolating target nucleic acidsequence using reca-like recombinase |
| US6482590B1 (en) | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
| US6117634A (en) | 1997-03-05 | 2000-09-12 | The Reagents Of The University Of Michigan | Nucleic acid sequencing and mapping |
| US6197557B1 (en) * | 1997-03-05 | 2001-03-06 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
| EP1705253B1 (en) | 1997-04-03 | 2010-11-03 | Life Technologies Corporation | Compositons and methods for reverse transcriptase-Polymerase chain reaction (RT-PCR) |
| WO1998045474A1 (en) | 1997-04-04 | 1998-10-15 | Innogenetics N.V. | Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions |
| US5928869A (en) * | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| TW589189B (en) | 1997-08-04 | 2004-06-01 | Scras | Kit containing at least one double-stranded RNA combined with at least one anti-viral agent for therapeutic use in the treatment of a viral disease, notably of viral hepatitis |
| GB9717061D0 (en) | 1997-08-13 | 1997-10-15 | Tepnel Medical Ltd | Amplification of nucleic acids |
| US6077669A (en) | 1997-11-04 | 2000-06-20 | Becton Dickinson And Company | Kit and method for fluorescence based detection assay |
| US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| JP4317953B2 (ja) * | 1998-01-22 | 2009-08-19 | 独立行政法人理化学研究所 | Ｄｎａの塩基配列決定方法 |
| US6686150B1 (en) | 1998-01-27 | 2004-02-03 | Clinical Micro Sensors, Inc. | Amplification of nucleic acids with electronic detection |
| GB9827152D0 (en) | 1998-07-03 | 1999-02-03 | Devgen Nv | Characterisation of gene function using double stranded rna inhibition |
| US6287825B1 (en) * | 1998-09-18 | 2001-09-11 | Molecular Staging Inc. | Methods for reducing the complexity of DNA sequences |
| WO2000028084A1 (en) | 1998-11-06 | 2000-05-18 | Molecular Biology Resources, Inc. | Isothermal nucleic acid amplification methods |
| EP2314700A1 (en) | 1999-01-28 | 2011-04-27 | Medical College of Georgia Research Institute, Inc | Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA |
| DE19956568A1 (de) | 1999-01-30 | 2000-08-17 | Roland Kreutzer | Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens |
| US6316200B1 (en) * | 2000-06-08 | 2001-11-13 | Becton, Dickinson And Company | Probes and methods for detection of nucleic acids |
| CA2386270A1 (en) | 1999-10-15 | 2001-04-26 | University Of Massachusetts | Rna interference pathway genes as tools for targeted genetic interference |
| US6893819B1 (en) * | 2000-11-21 | 2005-05-17 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| GB9927444D0 (en) | 1999-11-19 | 2000-01-19 | Cancer Res Campaign Tech | Inhibiting gene expression |
| US6783934B1 (en) | 2000-05-01 | 2004-08-31 | Cepheid, Inc. | Methods for quantitative analysis of nucleic acid amplification reaction |
| US6191267B1 (en) * | 2000-06-02 | 2001-02-20 | New England Biolabs, Inc. | Cloning and producing the N.BstNBI nicking endonuclease |
| DE10028837A1 (de) | 2000-06-15 | 2001-12-20 | Roche Diagnostics Gmbh | Verfahren zum Nachweis von Influenza A/B-Viren |
| US6350580B1 (en) * | 2000-10-11 | 2002-02-26 | Stratagene | Methods for detection of a target nucleic acid using a probe comprising secondary structure |
| AU2002232393A1 (en) * | 2000-10-27 | 2002-05-06 | Molecular Staging, Inc. | Methods for identifying genes associated with diseases or specific phenotypes |
| US6861222B2 (en) * | 2000-11-09 | 2005-03-01 | Yale University | Nucleic acid detection using structured probes |
| US7309573B2 (en) * | 2000-11-21 | 2007-12-18 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
| US6660475B2 (en) | 2000-12-15 | 2003-12-09 | New England Biolabs, Inc. | Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof |
| US6958217B2 (en) * | 2001-01-24 | 2005-10-25 | Genomic Expression Aps | Single-stranded polynucleotide tags |
| EP1386006B1 (en) | 2001-04-20 | 2008-05-28 | The Penn State Research Foundation | Methods for nucleic acid manipulation |
| CN1656233A (zh) | 2001-07-15 | 2005-08-17 | 凯克研究生院 | 利用切割剂扩增核酸片段 |
| CA2492423A1 (en) | 2001-07-15 | 2004-03-18 | Keck Graduate Institute | Exponential amplification of nucleic acids using nicking agents |
| WO2003008624A2 (en) | 2001-07-15 | 2003-01-30 | Keck Graduate Institute | Nucleic acid amplification using nicking agents |
| US6632611B2 (en) * | 2001-07-20 | 2003-10-14 | Affymetrix, Inc. | Method of target enrichment and amplification |
| US6403341B1 (en) | 2001-08-02 | 2002-06-11 | Wayne M. Barnes | Magnesium precipitate hot start method for PCR |
| AU2002327421A1 (en) | 2001-08-02 | 2003-02-17 | Wayne M. Barnes | Magnesium precipitate hot start method for molecular manipulation of nucleic acids |
| US20040038256A1 (en) | 2001-10-01 | 2004-02-26 | Keck Graduate Institute | Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization |
| US20040058349A1 (en) | 2001-10-01 | 2004-03-25 | Jeffrey Van Ness | Methods for identifying nucleotides at defined positions in target nucleic acids |
| US7297485B2 (en) | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
| US6977148B2 (en) | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
| US6617137B2 (en) * | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| DE60322044D1 (de) | 2002-02-21 | 2008-08-21 | Asm Scient Inc | Rekombinase-polymerase-amplifikation |
| GB0207063D0 (en) * | 2002-03-26 | 2002-05-08 | Amersham Biosciences Uk Ltd | Immobilised probes |
| US7662594B2 (en) * | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
| JP4632788B2 (ja) * | 2002-09-20 | 2011-02-16 | ニュー・イングランド・バイオラブズ・インコーポレイティッド | 核酸のヘリカーゼ依存性増幅 |
| US20060160759A1 (en) | 2002-09-28 | 2006-07-20 | Jianzhu Chen | Influenza therapeutic |
| WO2004067726A2 (en) | 2003-01-29 | 2004-08-12 | Keck Graduate Institute | Isothermal reactions for the amplification of oligonucleotides |
| WO2004067764A2 (en) | 2003-01-29 | 2004-08-12 | Keck Graduate Institute | Nucleic acid sequencing using nicking agents |
| US20040209299A1 (en) | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
| AU2004232976B2 (en) * | 2003-04-18 | 2011-02-10 | Becton, Dickinson And Company | Immuno-amplification |
| CA2523016C (en) * | 2003-04-25 | 2018-05-15 | Becton, Dickinson And Company | Detection of herpes simplex virus types 1 and 2 by nucleic acid amplification |
| WO2005026329A2 (en) | 2003-09-12 | 2005-03-24 | Cornell Research Foundation, Inc. | Methods for identifying target nucleic acid molecules |
| US20050074804A1 (en) * | 2003-09-26 | 2005-04-07 | Youxiang Wang | Amplification of polynucleotide sequences by rolling circle amplification |
| US7314714B2 (en) * | 2003-12-19 | 2008-01-01 | Affymetrix, Inc. | Method of oligonucleotide synthesis |
| EP2290106B1 (en) * | 2004-03-08 | 2018-01-03 | Rubicon Genomics, Inc. | Method for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation |
| US20050233332A1 (en) * | 2004-04-14 | 2005-10-20 | Collis Matthew P | Multiple fluorophore detector system |
| ES2425354T3 (es) | 2004-06-01 | 2013-10-14 | Alere San Diego, Inc. | Amplificación por recombinasa-polimerasa |
| GB2416352B (en) | 2004-07-21 | 2009-01-28 | Bioline Ltd | A method for performing the hot start of enzymatic reactions |
| KR20070085275A (ko) * | 2004-09-20 | 2007-08-27 | 유니버시티 오브 피츠버그 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 | 멀티플 모드에 의한 멀티플렉스 반응 억제 방법 |
| WO2006121773A2 (en) | 2005-05-06 | 2006-11-16 | Gen-Probe Incorporated | Compositions and assays to detect influenza virus a and b nucleic acids |
| US20070020639A1 (en) * | 2005-07-20 | 2007-01-25 | Affymetrix, Inc. | Isothermal locus specific amplification |
| JP5027126B2 (ja) | 2005-07-25 | 2012-09-19 | アリーア サン ディエゴ， インコーポレイテッド | リコンビナーゼポリメラーゼ増幅を多重化するための方法 |
| WO2007018601A1 (en) * | 2005-08-02 | 2007-02-15 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| EP1989318B1 (en) | 2006-01-06 | 2014-07-30 | Agilent Technologies, Inc. | Reaction buffer composition for nucleic acid replication with packed dna polymerases |
| WO2007095155A2 (en) | 2006-02-13 | 2007-08-23 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Primers and probes for detection and discrimination of types and subtypes of influenza viruses |
| ATE510027T1 (de) | 2006-02-27 | 2011-06-15 | Hoffmann La Roche | Pcr-heissstart durch magnesium-sequestrierung |
| CN100489112C (zh) | 2006-03-10 | 2009-05-20 | 杭州优思达生物技术有限公司 | 切口酶扩增靶核酸序列的方法及用于扩增靶核酸序列的试剂盒及其应用 |
| US20090092967A1 (en) * | 2006-06-26 | 2009-04-09 | Epoch Biosciences, Inc. | Method for generating target nucleic acid sequences |
| US20080096257A1 (en) | 2006-08-15 | 2008-04-24 | Zuxu Yao | Methods for Rapid, Single-Step Strand Displacement Amplification of Nucleic Acids |
| WO2009024834A2 (en) | 2006-12-05 | 2009-02-26 | Rosetta Genomics Ltd | Nucleic acids involved in viral infection |
| US8202972B2 (en) * | 2007-01-10 | 2012-06-19 | General Electric Company | Isothermal DNA amplification |
| US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| CA2786473A1 (en) | 2010-01-08 | 2011-07-14 | Qiagen Gaithersburg, Inc. | Materials and methods for isothermal nucleic acid amplification |
| KR101870311B1 (ko) | 2012-03-09 | 2018-06-25 | (주)바이오니아 | 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물 |
| WO2015113828A1 (en) | 2014-01-31 | 2015-08-06 | Qiagen Gmbh | Cation chelator hot start |
| WO2016004333A2 (en) | 2014-07-02 | 2016-01-07 | Promega Corporation | Reversible metal ion chelators |
| US10329601B2 (en) * | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
| CN111406118B (zh) * | 2017-08-31 | 2024-07-12 | 爱奥尼安技术有限责任公司 | 呼吸道合胞病毒种属的切口产生和延伸扩增反应(near) |
-
2007
- 2007-07-14 US US11/778,018 patent/US9689031B2/en active Active
-
2008
- 2008-07-14 ES ES12195331T patent/ES2816748T3/es active Active
- 2008-07-14 AU AU2008276118A patent/AU2008276118B2/en active Active
- 2008-07-14 DK DK12195331.9T patent/DK2657350T3/da active
- 2008-07-14 PT PT121953319T patent/PT2657350T/pt unknown
- 2008-07-14 WO PCT/US2008/070023 patent/WO2009012246A2/en not_active Ceased
- 2008-07-14 EP EP20174530.4A patent/EP3754029A1/en active Pending
- 2008-07-14 EP EP12195331.9A patent/EP2657350B1/en active Active
- 2008-07-14 CN CN201610524989.5A patent/CN106544409A/zh active Pending
- 2008-07-14 CA CA2693805A patent/CA2693805C/en active Active
- 2008-07-14 PL PL12195331T patent/PL2657350T3/pl unknown
- 2008-07-14 EP EP12195333.5A patent/EP2660333B2/en active Active
- 2008-07-14 DK DK08781827.4T patent/DK2181196T3/da active
- 2008-07-14 CN CN2008801054247A patent/CN101952459A/zh active Pending
- 2008-07-14 ES ES12195333T patent/ES2759872T5/es active Active
- 2008-07-14 EP EP08781827.4A patent/EP2181196B1/en not_active Revoked
- 2008-07-14 CA CA3041145A patent/CA3041145C/en active Active
- 2008-07-14 FI FIEP12195333.5T patent/FI2660333T4/fi active
- 2008-07-14 US US12/173,020 patent/US9617586B2/en active Active
- 2008-07-14 DK DK12195333.5T patent/DK2660333T4/da active
- 2008-07-14 CN CN201410465144.4A patent/CN104357546B/zh active Active
- 2008-07-14 ES ES08781827T patent/ES2434220T3/es active Active
- 2008-07-14 JP JP2010517111A patent/JP5693955B2/ja active Active
- 2008-07-14 BR BRPI0814527A patent/BRPI0814527B1/pt active IP Right Grant
- 2008-07-14 CN CN201410466581.8A patent/CN104372076B/zh active Active
-
2013
- 2013-10-30 US US14/067,623 patent/US9562264B2/en active Active
- 2013-10-30 US US14/067,620 patent/US9562263B2/en active Active
-
2014
- 2014-01-22 JP JP2014009503A patent/JP2014073137A/ja active Pending
-
2015
- 2015-05-06 AU AU2015202439A patent/AU2015202439C1/en active Active
-
2017
- 2017-03-23 US US15/467,893 patent/US20180023130A1/en not_active Abandoned
- 2017-05-22 US US15/600,951 patent/US10851406B2/en active Active
- 2017-11-29 AU AU2017268587A patent/AU2017268587A1/en not_active Abandoned
-
2019
- 2019-01-09 US US16/243,829 patent/US20190323072A1/en not_active Abandoned
-
2020
- 2020-01-16 AU AU2020200321A patent/AU2020200321B2/en active Active
-
2021
- 2021-08-11 US US17/399,482 patent/US20210388425A1/en not_active Abandoned
-
2022
- 2022-08-26 AU AU2022221561A patent/AU2022221561A1/en not_active Abandoned
-
2024
- 2024-07-30 US US18/788,585 patent/US20240409987A1/en active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK2660333T4 (da) | Nicking og udvidelsesamplificeringsreaktion til exponentiel amplificering af nukleinsyrer | |
| DK3272879T3 (da) | Transposonendesammensætninger og fremgangsmåder til modificering af nukleinsyrer | |
| CY2019044I1 (el) | Αναστολεις διυδροπυριδοφθαλαζινονης της πολυμερασης της πολυαδενοσφωσφορικης ριβοζης (parp) | |
| DK3133169T3 (da) | Fremgangsmåder til selektion og amplificering af polynukleotider | |
| EP2294076A4 (en) | CHEMICALLY MODIFIED NUCLEOSIDE 5'-TRIPHOSPHATES FOR HEAT-INITIATED NUCLEIC ACID AMPAYMENT | |
| DK2414547T3 (da) | Multiprimer-amplifikationsmetode til stregkodning af målnukleinsyrer | |
| DK3375891T3 (da) | Fremgangsmåder og indretninger til kompensation af spektral krydstale i multipleks-nukleinsyreamplifikation | |
| DK3428290T3 (da) | Sammensætninger og fremgangsmåder til amplifikation af nukleinsyrer | |
| DK2255551T3 (da) | Lydsystem og fremgangsmåde til drift deraf | |
| EP2352818A4 (en) | CHIMERIC DNA POLYMERASES | |
| DK3208269T3 (da) | Inhibitorer af c-fms-kinase | |
| DK2601611T3 (da) | Fremgangsmåder til forudsigelse af stabilitet og smeltetemperaturer for nukleinsyreduplekser | |
| DK3165234T3 (da) | Rna med en kombination af umodificerede og modificerede nukleotider til proteinekspression | |
| DK3012329T3 (da) | Fremgangsmåder og materialer til at vurdere tab af heterozygositet | |
| DK2325648T3 (da) | Fremgangsmåde til detektering af cancer | |
| DK3252057T3 (da) | Multicykliske forbindelser og fremgangsmåder til anvendelse deraf | |
| BRPI0811755A2 (pt) | Amplificação específica de senquência de dna específicas de tumor. | |
| DK2470656T3 (da) | Sammensætning til hæmning af genekspression og anvendelser heraf | |
| BRPI0908748A2 (pt) | Composições e métodos para análise de moléculas de ácido nucleico durante reações de amplificação | |
| EP2207899A4 (en) | ASSEMBLY OF LARGE SIZED NUCLEIC ACIDS | |
| DK2029746T3 (da) | Sammensætninger og fremgangsmåder til siRNA-hæmning af angiogenese | |
| BRPI0814797A2 (pt) | Inibidores dna-pk | |
| ZA201105632B (en) | Method of amplification of gc-rich dna templates | |
| DK2141172T3 (da) | Cyclopropylpolymerase inhibitorer | |
| DK3587579T3 (da) | Fremgangsmåder og sammensætninger til specifik hæmning af beta-catenin ved hjælp af dobbeltstrenget rna |