ATE510027T1 - Pcr-heissstart durch magnesium-sequestrierung - Google Patents

Pcr-heissstart durch magnesium-sequestrierung

Info

Publication number
ATE510027T1
ATE510027T1 AT07703556T AT07703556T ATE510027T1 AT E510027 T1 ATE510027 T1 AT E510027T1 AT 07703556 T AT07703556 T AT 07703556T AT 07703556 T AT07703556 T AT 07703556T AT E510027 T1 ATE510027 T1 AT E510027T1
Authority
AT
Austria
Prior art keywords
hot start
pcr hot
sequestration
magnesium
present
Prior art date
Application number
AT07703556T
Other languages
English (en)
Inventor
Waltraud Ankenbauer
Dieter Heindl
Frank Laue
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE510027T1 publication Critical patent/ATE510027T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Compounds Of Alkaline-Earth Elements, Aluminum Or Rare-Earth Metals (AREA)
  • Oscillators With Electromechanical Resonators (AREA)
AT07703556T 2006-02-27 2007-02-23 Pcr-heissstart durch magnesium-sequestrierung ATE510027T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06003903 2006-02-27
PCT/EP2007/001585 WO2007096182A1 (en) 2006-02-27 2007-02-23 Pcr hot start by magnesium sequestration

Publications (1)

Publication Number Publication Date
ATE510027T1 true ATE510027T1 (de) 2011-06-15

Family

ID=36423585

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07703556T ATE510027T1 (de) 2006-02-27 2007-02-23 Pcr-heissstart durch magnesium-sequestrierung

Country Status (8)

Country Link
US (1) US8026058B2 (de)
EP (1) EP1989324B1 (de)
JP (1) JP5022383B2 (de)
CN (2) CN101421417B (de)
AT (1) ATE510027T1 (de)
CA (1) CA2638854C (de)
ES (1) ES2364983T3 (de)
WO (1) WO2007096182A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US7910720B2 (en) * 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
EP2436777B1 (de) 2009-05-26 2015-08-19 Xiamen University Verfahren zur erkennung von variationen in nukleinsäuresequenzen
JP5798631B2 (ja) 2010-10-04 2015-10-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｒｔ−ｐｃｒ反応緩衝液中での細胞溶解のための方法
CN103124796B (zh) 2010-10-04 2016-08-03 霍夫曼-拉罗奇有限公司 用于pcr反应缓冲液中的细胞裂解的方法
CA2810316A1 (en) 2010-10-04 2012-04-12 F. Hoffmann-La Roche Ag Method for cell lysis and pcr within the same reaction vessel
EP2859111B1 (de) * 2012-06-08 2020-07-08 Ionian Technologies, LLC Nukleinsäureamplifizierungen
EP3099810B1 (de) 2014-01-31 2019-05-01 Qiagen GmbH Kationen-chelator-warmstart
WO2016004333A2 (en) 2014-07-02 2016-01-07 Promega Corporation Reversible metal ion chelators
CN105755119A (zh) * 2016-03-14 2016-07-13 江西中大赛尔生物技术股份有限公司 一种pcr热启动方法
CN106544340A (zh) * 2016-12-08 2017-03-29 山西大学 一种适于高at含量dna的pcr扩增体系及方法

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US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5449603A (en) 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
CA2035010C (en) 1990-01-26 1996-12-10 Keith C. Backman Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
DK0515506T3 (da) 1990-02-16 2000-05-08 Hoffmann La Roche Fremgangsmåde til påvisning af carcinogene humane papillomavirus
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
GB2293238A (en) 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US6033851A (en) 1994-12-09 2000-03-07 Wakunaga Seiyaku Kabushiki Kaisha Method for suppressing nonspecific hybridization in primer extension method
CA2168712A1 (en) * 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
CA2176193A1 (en) 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
US6020130A (en) 1995-06-07 2000-02-01 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands that bind to and inhibit DNA polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6416948B1 (en) 1996-06-03 2002-07-09 The Governors Of The University Of Alberta Methods for detection of rearranged DNA
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
ES2253778T3 (es) 1996-06-04 2006-06-01 University Of Utah Research Foundation Sistema y metodos de monitorizacion de una amplificacion de adn mediante fluorescencia.
US6617114B1 (en) * 1996-10-31 2003-09-09 Karo Bio Ab Identification of drug complementary combinatorial libraries
EP0930370B1 (de) 1997-12-15 2007-08-08 CSL Behring GmbH Markierter Primer, geeignet für die Detektion von Nukleinsäuren
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
DE19913923A1 (de) 1999-03-26 2000-09-28 Amphenol Tuchel Elect Kontaktiereinrichtung für eine SIM-Karte
EP1175501A4 (de) 1999-05-12 2002-11-27 Invitrogen Corp Zusammensetzungen und verfahren zur erhöhung der empfindlichkeit und spezifität der nukleinsäuresynthese
WO2002014555A2 (en) 2000-08-11 2002-02-21 University Of Utah Research Foundation Single-labeled oligonucleotide probes
US6746104B2 (en) * 2000-09-25 2004-06-08 Picoliter Inc. Method for generating molecular arrays on porous surfaces
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
US6403341B1 (en) * 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases

Also Published As

Publication number Publication date
HK1186742A1 (en) 2014-03-21
EP1989324B1 (de) 2011-05-18
JP5022383B2 (ja) 2012-09-12
JP2009528283A (ja) 2009-08-06
US8026058B2 (en) 2011-09-27
EP1989324A1 (de) 2008-11-12
CN103172700B (zh) 2014-12-17
CA2638854C (en) 2012-05-15
US20100285535A1 (en) 2010-11-11
CA2638854A1 (en) 2007-08-30
ES2364983T3 (es) 2011-09-20
WO2007096182A1 (en) 2007-08-30
CN101421417B (zh) 2013-04-03
CN101421417A (zh) 2009-04-29
CN103172700A (zh) 2013-06-26

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