DK267889A - Acridiniumestermaerkning og rensning af nucleotidprober - Google Patents

Acridiniumestermaerkning og rensning af nucleotidprober Download PDF

Info

Publication number: DK267889A
Authority: DK; Denmark
Prior art keywords: probe; acridinium; label; acridinium ester; solution
Prior art date: 1987-10-05

Application number

DK267889A

Other languages

English (en)

Other versions

DK267889D0 (da

Inventor

Lyle John Arnold

Norman Charles Nelson

Original Assignee

Ml Technology Ventures

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-10-05

Filing date

1989-06-01

Publication date

1989-08-01

1989-06-01 Application filed by Ml Technology Ventures filed Critical Ml Technology Ventures

1989-06-01 Publication of DK267889D0 publication Critical patent/DK267889D0/da

1989-08-01 Publication of DK267889A publication Critical patent/DK267889A/da

Links

239000000523 sample Substances 0.000 title abstract 9
DZBUGLKDJFMEHC-UHFFFAOYSA-O acridine;hydron Chemical compound C1=CC=CC2=CC3=CC=CC=C3[NH+]=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-O 0.000 title 1
238000004140 cleaning Methods 0.000 title 1
239000002773 nucleotide Substances 0.000 title 1
RXNXLAHQOVLMIE-UHFFFAOYSA-N phenyl 10-methylacridin-10-ium-9-carboxylate Chemical compound C12=CC=CC=C2[N+](C)=C2C=CC=CC2=C1C(=O)OC1=CC=CC=C1 RXNXLAHQOVLMIE-UHFFFAOYSA-N 0.000 abstract 3
238000000034 method Methods 0.000 abstract 2
238000000746 purification Methods 0.000 abstract 2
238000000926 separation method Methods 0.000 abstract 2
108020004711 Nucleic Acid Probes Proteins 0.000 abstract 1
DZBUGLKDJFMEHC-UHFFFAOYSA-N acridine Chemical class C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 abstract 1
-1 acridinium esters 4-(2-succinimidyloxycarbonyl-ethyl)phenyl10-methylacridinium-9-carboxy late fluorosulfonate Chemical class 0.000 abstract 1
238000010276 construction Methods 0.000 abstract 1
238000004128 high performance liquid chromatography Methods 0.000 abstract 1
229910052588 hydroxylapatite Inorganic materials 0.000 abstract 1
238000005342 ion exchange Methods 0.000 abstract 1
238000002372 labelling Methods 0.000 abstract 1
239000002853 nucleic acid probe Substances 0.000 abstract 1
239000003960 organic solvent Substances 0.000 abstract 1
XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 abstract 1
150000003141 primary amines Chemical class 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Engineering & Computer Science (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Investigating Or Analysing Biological Materials (AREA)
Peptides Or Proteins (AREA)
Polarising Elements (AREA)
Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
Plural Heterocyclic Compounds (AREA)

DK267889A 1987-10-05 1989-06-01 Acridiniumestermaerkning og rensning af nucleotidprober DK267889A (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US10508087A	1987-10-05	1987-10-05
PCT/US1988/003361 WO1989002896A1 (en)	1987-10-05	1988-10-05	Acridinium ester labelling and purification of nucleotide probes

Publications (2)

Publication Number	Publication Date
DK267889D0 DK267889D0 (da)	1989-06-01
DK267889A true DK267889A (da)	1989-08-01

Family

ID=22303939

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK267889A DK267889A (da)	1987-10-05	1989-06-01	Acridiniumestermaerkning og rensning af nucleotidprober

Country Status (11)

Country	Link
EP (1)	EP0312248B1 (da)
JP (1)	JPH02502283A (da)
KR (1)	KR970005899B1 (da)
AT (1)	ATE109790T1 (da)
CA (1)	CA1314009C (da)
DE (1)	DE3851031T2 (da)
DK (1)	DK267889A (da)
ES (1)	ES2056937T3 (da)
FI (1)	FI892692A0 (da)
PT (1)	PT88665B (da)
WO (1)	WO1989002896A1 (da)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4948882A (en) *	1983-02-22	1990-08-14	Syngene, Inc.	Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
GB8902689D0 (en) *	1989-02-07	1989-03-30	Ici Plc	Assay method
US5082780A (en) *	1989-09-12	1992-01-21	Eastman Kodak Company	Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures
NZ239252A (en) *	1990-08-09	1997-07-27	Genta Inc	Reagent for attaching a psoralen-containing moiety to an oligonucleotide(derivative); oligonucleotide(derivative)-psoralen conjugates
EP0602524A1 (de) *	1992-12-15	1994-06-22	Hoechst Aktiengesellschaft	Chemilumineszenzmarkierte Gensonden und ihre Verwendung in Gensondentesten
US5739309A (en) *	1993-07-19	1998-04-14	Gen-Probe Incorporated	Enhancement of oligonucleotide inhibition of protein production, cell proliferation and / or multiplication of infectious disease pathogens
JP3789471B2 (ja) *	1993-07-19	2006-06-21	ジェン−プローブ・インコーポレイテッド	オリゴヌクレオチドスクリーニングアッセイ
AU688364B2 (en) *	1993-07-19	1998-03-12	Gen-Probe Incorporated	Oligonucleotides with activity against human immunodeficiency virus
GB2282222A (en) *	1993-09-09	1995-03-29	Univ Nottingham	Detecting genetic material using labelled DNA
US5783453A (en) *	1995-06-29	1998-07-21	Chiron Diagnostics Corporation	Non-separation specific binding chemiluminescent assay
DE19534122A1 (de) *	1995-09-14	1997-03-20	Behringwerke Ag	Homogener Gensondentest mit einem gegen das Label gerichteten Rezeptor
DE19544297A1 (de) *	1995-11-28	1997-06-05	Behringwerke Ag	Verfahren zur Entfernung von aromatischen Verbindungen aus produkthaltigen Lösungen
AU726047B2 (en) *	1995-11-15	2000-10-26	Gen-Probe Incorporated	Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits
JP5514401B2 (ja)	2005-09-21	2014-06-04	国立大学法人広島大学	Ｇテイル配列の長さ測定方法及びそれに用いるキット
CN109852669A (zh) *	2019-01-04	2019-06-07	南京航思生物科技有限公司	一种吖啶酯标记核酸的方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FR2540122B1 (fr) *	1983-01-27	1985-11-29	Centre Nat Rech Scient	Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application
CA1307480C (en) *	1985-07-10	1992-09-15	Nanibhushan Dattagupta	Prolonged chemiluminescence
JP2509574B2 (ja) *	1985-08-15	1996-06-19	アモコ・コーポレーション	標識付けした核酸
JP3293820B2 (ja) *	1985-12-13	2002-06-17	エンゾ− バイオケムインコ−ポレイテツド	標的ポリヌクレオチドにハイブリツド形成するための新規な一工程方法とポリヌクレオチド化合物
US4855225A (en) *	1986-02-07	1989-08-08	Applied Biosystems, Inc.	Method of detecting electrophoretically separated oligonucleotides
EP0310312A3 (en) *	1987-10-02	1990-08-16	M.L. Technology Ventures, L.P.	Non-nucleotide reagents for substituting termini of oligonucleotides

1988
- 1988-10-04 PT PT88665A patent/PT88665B/pt not_active IP Right Cessation
- 1988-10-05 JP JP63508511A patent/JPH02502283A/ja active Pending
- 1988-10-05 AT AT88309283T patent/ATE109790T1/de active
- 1988-10-05 CA CA000579422A patent/CA1314009C/en not_active Expired - Lifetime
- 1988-10-05 ES ES88309283T patent/ES2056937T3/es not_active Expired - Lifetime
- 1988-10-05 WO PCT/US1988/003361 patent/WO1989002896A1/en not_active Ceased
- 1988-10-05 EP EP88309283A patent/EP0312248B1/en not_active Expired - Lifetime
- 1988-10-05 DE DE3851031T patent/DE3851031T2/de not_active Expired - Fee Related
1989
- 1989-06-01 FI FI892692A patent/FI892692A0/fi not_active Application Discontinuation
- 1989-06-01 DK DK267889A patent/DK267889A/da not_active Application Discontinuation
- 1989-06-03 KR KR1019890700991A patent/KR970005899B1/ko not_active Expired - Fee Related

Also Published As

Publication number	Publication date
PT88665B (pt)	1992-12-31
FI892692A7 (fi)	1989-06-01
JPH02502283A (ja)	1990-07-26
KR890701611A (ko)	1989-12-21
EP0312248B1 (en)	1994-08-10
KR970005899B1 (ko)	1997-04-21
WO1989002896A1 (en)	1989-04-06
DE3851031T2 (de)	1994-12-01
ES2056937T3 (es)	1994-10-16
DK267889D0 (da)	1989-06-01
EP0312248A2 (en)	1989-04-19
FI892692A0 (fi)	1989-06-01
CA1314009C (en)	1993-03-02
EP0312248A3 (en)	1991-01-09
DE3851031D1 (de)	1994-09-15
ATE109790T1 (de)	1994-08-15

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Legal Events

Date	Code	Title	Description
1992-06-29	AHB	Application shelved due to non-payment