DK2700721T3 - Muterede acetohydroxysyresyntasegener i brassica - Google Patents
Muterede acetohydroxysyresyntasegener i brassica Download PDFInfo
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- DK2700721T3 DK2700721T3 DK13175389.9T DK13175389T DK2700721T3 DK 2700721 T3 DK2700721 T3 DK 2700721T3 DK 13175389 T DK13175389 T DK 13175389T DK 2700721 T3 DK2700721 T3 DK 2700721T3
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Claims (15)
1. Fremgangsmåde til fremstilling af en herbicidresistent plante, hvilken fremgangsmåde omfatter: (a) at indføre i en plantecelle en genreparation-oligonukleobase (GRON) med en målrettet mutation i et acetohydroxysyresyntase (AHAS)-gen til fremstilling af en plantecelle med: (i) et AHAS-gen, der udtrykker et AHAS-protein omfattende en substitution af asparaginsyre med glutaminsyre ved aminosyrepositionen svarende til position D376 af SEQ ID NO: 1 og (ii) et AHAS-gen, der udtrykker et AHAS-protein omfattende en substitution af serin med asparagin ved aminosyrepositionen svarende til position 653 af SEQ ID NO: 1; (b) at identificere en plantecelle med i det væsentlige normal vækst og katalytisk aktivitet i forhold til en tilsvarende vildtypeplantecelle i nærvær af et AHAS-hæmmende herbicid; og (c) at regenerere en ikke-transgen herbicidresistent plante med et muteret AHAS-gen fra plantecellen.
2. Fremgangsmåde ifølge krav 1, hvor genreparation-oligonukleobasen er en enkeltstrenget oligodeoxynukleotid-mutationsvektor.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor AHAS-proteinet er modstandsdygtigt over for hæmning gennem et AHAS-hæmmende herbicid, især hvor det AHAS-hæmmende herbicid udvælges fra gruppen bestående af herbicider af: imidazolinon, sulfonylurea, triazolopyrimidin, pyrimidinylthiobenzoat, sul-fonylamino-carbonyltriazolinon og blandinger deraf.
4. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor AHAS-genet koder for et protein omfattende 70 % identitet eller derover med en eller flere af aminosyresekvenserne fra figur 2.
5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor planten er udvalgt blandt plantearter fra gruppen bestående af canola, solsikke, tobak, sukkerroe, bomuld, majs, hvede, byg, ris, hirse, tomat, mango, fersken, æble, pære, jordbær, banan, melon, kartoffel, gulerod, salat, løg, soja arter, sukkerrør, ærter, hestebønner, poppel, vindruer, citrus, lucerne, rug, havre, græstørv og fodergræsser, hør, raps, agurk, ipomoea, balsamin, peber, aubergine, morgenfrue, lotus, kål, bellis, nellike, tulipan, iris, lilje og nøddepro-ducerende planter.
6. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor planten er en Brassica napus-plante.
7. Plante omfattende et acetohydroxysyresyntase (AHAS)-gen, hvor genet koder for et AHAS-protein omfattende en substitution af asparaginsyre med glutaminsyre ved aminosyrepositionen svarende til position D376 af SEQ ID NO: 1, og en substitution af serin med asparagin ved aminosyrepositionen svarende til position 653 af SEQ ID NO: 1, og hvor planten ikke udelukkende opnås ved hjælp af en i det væsentlige biologisk proces.
8. Plante ifølge krav 7, hvor planten er ikke-transgen, og hvor planten ikke udelukkende opnås ved hjælp af en i det væsentlige biologisk proces.
9. Plante ifølge krav 7 eller 8, hvor AHAS-proteinet er modstandsdygtigt over for hæmning gennem et AHAS-hæmmende herbicid.
10. Plante ifølge et hvilket som helst af kravene 7 til 9, hvor AHAS-genet koder for et protein omfattende 70 % identitet eller derover med en eller flere af ami-nosyresekvenserne fra figur 2.
11. Plante ifølge et hvilket som helst af kravene 7 til 10, hvor planten er udvalgt blandt plantearter fra gruppen bestående af canola, solsikke, tobak, sukkerroe, bomuld, majs, hvede, byg, ris, hirse, tomat, mango, fersken, æble, pære, jordbær, banan, melon, kartoffel, gulerod, salat, løg, soja-arter, sukkerrør, ærter, hestebønner, poppel, vindruer, citrus, lucerne, rug, havre, græstørv og fodergræsser, hør, raps, agurk, ipomoea, balsamin, peber, aubergine, morgenfrue, lotus, kål, bellis, nellike, tulipan, iris, lilje og nøddeproducerende planter.
12. Plante ifølge et hvilket som helst af kravene 7 til 11, hvor planten er en Brassica napus-plante.
13. Frø produceret af planten ifølge et hvilket som helst af kravene 7 til 12, hvor frøet omfatter et acetohydroxysyresyntase (AHAS)-gen, der koder for et AHAS-protein omfattende en substitution af asparaginsyre med glutaminsyre ved aminosyrepositionen svarende til position D376 af SEQ ID NO: 1, og en substitution af serin med asparagin ved aminosyrepositionen svarende til position 653 af SEQ ID NO: 1, og hvor frøet ikke udelukkende opnås ved hjælp af en i det væsentlige biologisk proces.
14. Isoleret nukleinsyre, der koder for et acetohydroxysyresyntase (AHAS)-protein omfattende en substitution af asparaginsyre med glutaminsyre ved aminosyrepositionen svarende til position D376 af SEQ ID NO: 1, og en substitution af serin med asparagin ved aminosyrepositionen svarende til position 653 af SEQ ID NO: 1.
15. Ekspressionsvektor omfattende en nukleinsyre, der koder for et acetohydroxysyresyntase (AHAS)-protein omfattende en substitution af asparaginsyre med glutaminsyre ved aminosyrepositionen svarende til position D376 af SEQ ID NO: 1, og en substitution af serin med asparagin ved aminosyrepositionen svarende til position 653 af SEQ ID NO: 1.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US97794407P | 2007-10-05 | 2007-10-05 | |
| EP08836084.7A EP2203565B1 (en) | 2007-10-05 | 2008-10-03 | Mutated acetohydroxyacid synthase genes in brassica |
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| DK2700721T3 true DK2700721T3 (da) | 2019-04-01 |
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| DK08836084.7T DK2203565T3 (da) | 2007-10-05 | 2008-10-03 | Muterede acetohydroxysyresyntasegener i brassica |
| DK13175393.1T DK2700722T3 (da) | 2007-10-05 | 2008-10-03 | Muterede acetohydroxysyresyntasegener i brassica |
| DK13175389.9T DK2700721T3 (da) | 2007-10-05 | 2008-10-03 | Muterede acetohydroxysyresyntasegener i brassica |
Family Applications Before (2)
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| DK08836084.7T DK2203565T3 (da) | 2007-10-05 | 2008-10-03 | Muterede acetohydroxysyresyntasegener i brassica |
| DK13175393.1T DK2700722T3 (da) | 2007-10-05 | 2008-10-03 | Muterede acetohydroxysyresyntasegener i brassica |
Country Status (13)
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| US (3) | US20090205064A1 (da) |
| EP (4) | EP2700722B1 (da) |
| JP (4) | JP5745849B2 (da) |
| CN (5) | CN108130336A (da) |
| AU (1) | AU2008308530A1 (da) |
| BR (3) | BR122018070230B1 (da) |
| CA (1) | CA2701624C (da) |
| DK (3) | DK2203565T3 (da) |
| EA (3) | EA202092479A3 (da) |
| LT (2) | LT2700722T (da) |
| PL (3) | PL2700721T3 (da) |
| UA (2) | UA103887C2 (da) |
| WO (1) | WO2009046334A1 (da) |
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| UA108733C2 (uk) | 2006-12-12 | 2015-06-10 | Толерантна до гербіциду рослина соняшника | |
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| KR20140050759A (ko) | 2007-12-21 | 2014-04-29 | 키진 엔.브이. | 식물 원형질체 내로 폴리에틸렌 글리콜 매개 돌연변이 뉴클레오염기의 도입을 이용한 개선된 돌연변이 생성방법 |
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| UA112969C2 (uk) * | 2010-08-03 | 2016-11-25 | Сібас Юс Ллс | Рослина, стійка до одного або більше ррх-інгібуючих гербіцидів, яка містить мутантний ген протопорфіриноген ix оксидази (ррх) |
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| PL2627183T5 (pl) | 2010-10-15 | 2024-02-05 | Bayer Cropscience Aktiengesellschaft | Zastosowanie herbicydów inhibitorów ALS do kontroli niechcianej roślinności w roślinach Beta vulgaris z tolerancją herbicydów inhibitorów ALS |
| UA113721C2 (uk) | 2010-10-15 | 2017-03-10 | Баєр Інтеллекчуел Проперті Гмбх | Толерантний до інгібуючого als гербіциду мутант буряку звичайного |
| UA117447C2 (uk) * | 2011-05-04 | 2018-08-10 | Байєр Інтеллектуал Проперті Гмбх | Застосування гербіцидів інгібіторів als для боротьби з небажаною рослинністю на площі вирощування brassica |
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| CA2932044A1 (en) * | 2013-12-02 | 2015-06-11 | Bayer Cropscience Nv | Als inhibitor herbicide tolerant mutant plants |
| KR102450868B1 (ko) | 2014-03-14 | 2022-10-06 | 시버스 유에스 엘엘씨 | 올리고뉴클레오타이드 매개 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물 |
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| CN104789682B (zh) * | 2015-04-29 | 2019-05-17 | 江苏省农业科学院 | 检测甘蓝型油菜抗磺酰脲类除草剂基因BnALS3R的引物与应用 |
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| CN107058350A (zh) * | 2017-03-20 | 2017-08-18 | 江苏省农业科学院 | 基于体外定点突变获得的油菜抗多种als抑制剂类除草剂基因及应用 |
| KR101996129B1 (ko) | 2017-07-11 | 2019-07-04 | 씨제이제일제당 (주) | 아세토하이드록시산 신타아제 변이체, 이를 포함하는 미생물 또는 이를 이용하는 l-분지쇄 아미노산 생산 방법 |
| US11021697B2 (en) | 2017-07-11 | 2021-06-01 | Cj Cheiljedang Corporation | Acetohydroxy acid synthase variant, microorganism comprising the same, and method of producing L-branched-chain amino acid using the same |
| CN109197576A (zh) * | 2018-11-16 | 2019-01-15 | 贵州省油料研究所 | 一种甘蓝油菜隐性核不育系与百合杂交选育油菜的方法 |
| CN109880927A (zh) * | 2019-03-20 | 2019-06-14 | 江苏省农业科学院 | 检测油菜BnALS1R基因的SNP标记引物及其应用 |
| CN110628783A (zh) * | 2019-10-23 | 2019-12-31 | 江苏省农业科学院 | 一种非转基因抗除草剂油菜基因及其应用 |
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| CN115992155B (zh) * | 2023-02-09 | 2025-09-12 | 中国农业科学院油料作物研究所 | 一种抗磺酰脲类除草剂油菜基因、蛋白、引物组、重组表达载体、工程菌及其应用 |
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- 2008-10-03 DK DK13175389.9T patent/DK2700721T3/da active
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