DK2718416T3 - Ekspansion af stamceller i hulfiberbioreaktorer - Google Patents

Ekspansion af stamceller i hulfiberbioreaktorer Download PDF

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Publication number
DK2718416T3
DK2718416T3 DK12727636.8T DK12727636T DK2718416T3 DK 2718416 T3 DK2718416 T3 DK 2718416T3 DK 12727636 T DK12727636 T DK 12727636T DK 2718416 T3 DK2718416 T3 DK 2718416T3
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Prior art keywords
expansion
stem cells
hollow fiber
fiber bioreactors
bioreactors
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DK12727636.8T
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English (en)
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Jozef Albert Martha Pinxteren
David Craeye
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ReGenesys BVBA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/10—Hollow fibers or tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Epidemiology (AREA)
  • Rheumatology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK12727636.8T 2011-06-06 2012-06-06 Ekspansion af stamceller i hulfiberbioreaktorer DK2718416T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161493737P 2011-06-06 2011-06-06
PCT/EP2012/060690 WO2012168295A1 (en) 2011-06-06 2012-06-06 Expansion of stem cells in hollow fiber bioreactors

Publications (1)

Publication Number Publication Date
DK2718416T3 true DK2718416T3 (da) 2020-02-24

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Family Applications (1)

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DK12727636.8T DK2718416T3 (da) 2011-06-06 2012-06-06 Ekspansion af stamceller i hulfiberbioreaktorer

Country Status (15)

Country Link
US (3) US20120308531A1 (da)
EP (2) EP2718416B1 (da)
JP (6) JP6182135B2 (da)
KR (2) KR101955053B1 (da)
CN (2) CN103732733A (da)
AU (3) AU2012266404B2 (da)
BR (1) BR112013031368B1 (da)
CA (1) CA2837419C (da)
DK (1) DK2718416T3 (da)
ES (1) ES2763331T3 (da)
HU (1) HUE047474T2 (da)
IL (2) IL229608A0 (da)
PL (1) PL2718416T3 (da)
SG (3) SG10201914092XA (da)
WO (1) WO2012168295A1 (da)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2245141B1 (en) 2008-01-18 2018-03-14 Regents of the University of Minnesota Stem cell aggregates and methods for making and using
WO2011143415A1 (en) 2010-05-12 2011-11-17 Abt Holding Company Modulation of splenocytes in cell therapy
EP2625264B1 (en) 2010-10-08 2022-12-07 Terumo BCT, Inc. Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions
EP2718416B1 (en) * 2011-06-06 2019-11-13 Regenesys bvba Expansion of stem cells in hollow fiber bioreactors
JP6666240B2 (ja) 2013-04-12 2020-03-13 フランチェスカ， サベリオ ラ 移植のための臓器の改良
JP6633522B2 (ja) 2013-11-16 2020-01-22 テルモ ビーシーティー、インコーポレーテッド バイオリアクターにおける細胞増殖
NL2011895C2 (en) * 2013-12-04 2015-06-08 Mikhail Alexandrovich Ponomarenko Fluidic device and perfusion system for in vitro tissue reconstruction.
DE102013226042A1 (de) * 2013-12-16 2015-06-18 Helmholtz-Zentrum Dresden - Rossendorf E.V. Vorrichtung und Verfahren zur Gewinnung von 2- und 3-wertigen Metallionen aus primären und sekundären Rohstoffen mit Hilfe von mikrobiellen Metaboliten
WO2015148704A1 (en) 2014-03-25 2015-10-01 Terumo Bct, Inc. Passive replacement of media
JP6510649B2 (ja) 2014-08-28 2019-05-08 ステモニックス インコーポレイティド 細胞アレイの製造方法及び使用方法
US20160090569A1 (en) * 2014-09-26 2016-03-31 Terumo Bct, Inc. Scheduled Feed
WO2016121773A1 (ja) * 2015-01-26 2016-08-04 宇部興産株式会社 ポリイミド多孔質膜を用いる細胞の大量培養方法、装置及びキット
US20160326494A1 (en) 2015-05-05 2016-11-10 Katholieke Universiteit Leuven Methods for Pancreatic Islet Transplantation
CN107750271B (zh) * 2015-05-13 2021-12-10 泰尔茂比司特公司 细胞扩增
US11248212B2 (en) 2015-06-30 2022-02-15 StemoniX Inc. Surface energy directed cell self assembly
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
WO2017066663A1 (en) * 2015-10-15 2017-04-20 Carromeu Cassiano Method of manufacturing cells using a hollow fiber bioreactor
KR20180102661A (ko) 2016-01-21 2018-09-17 에이비티 홀딩 컴퍼니 상처 치유를 위한 줄기 세포
AU2016397306B2 (en) * 2016-03-14 2022-02-03 Omnibrx Biotechnologies Private Limited A bioreactor system and method thereof
CN105754948A (zh) * 2016-04-15 2016-07-13 浙江大学 结肠癌细胞的体外拟组织化培养方法
CN109689366B (zh) 2016-05-05 2022-12-02 西南研究院 用于细胞扩增和相关应用的三维生物反应器
JP7034949B2 (ja) * 2016-05-25 2022-03-14 テルモ ビーシーティー、インコーポレーテッド 細胞の増殖
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
FR3053051B1 (fr) 2016-06-24 2022-11-11 Cellprothera Cassette et automate de culture cellulaire
CN108384717A (zh) 2017-02-03 2018-08-10 财团法人工业技术研究院 细胞培养载体模块与细胞培养系统
US12234441B2 (en) 2017-03-31 2025-02-25 Terumo Bct, Inc. Cell expansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
CN117247899A (zh) 2017-03-31 2023-12-19 泰尔茂比司特公司 细胞扩增
US12281330B2 (en) 2017-09-11 2025-04-22 SymbioCellTech Adaptation of hollow-fiber-based cell culture technology for the manufacturing of (1) neo-islets, employed for the treatment of type 1 and type 2 diabetes mellitus, and (2) the generation of exosomes from various cell types, used in the treatment of different organ injuries and diseases
AU2019206643A1 (en) * 2018-01-12 2020-07-23 Rutgers, The State University Of New Jersey Cell reprogramming therapy
US11149244B2 (en) 2018-04-04 2021-10-19 Southwest Research Institute Three-dimensional bioreactor for T-cell activation and expansion for immunotherapy
EP3794104A4 (en) * 2018-05-18 2022-06-08 Children's Research Institute, Children's National Medical Center CELL EXPANSION SYSTEM AND METHODS OF USING THE SAME
FR3081168B1 (fr) * 2018-05-21 2022-03-11 Univ Bordeaux Systeme de culture cellulaire en bioreacteur
SG11202102986XA (en) 2018-09-24 2021-04-29 Southwest Res Inst Three-dimensional bioreactors
US11926545B2 (en) * 2018-11-08 2024-03-12 University Of Notre Dame Du Lac Catalytic hydrogel membrane reactor for treatment of aqueous contaminants
CN109679834B (zh) * 2019-01-07 2020-06-05 赵涌 用于体外规模化生产红细胞的中空纤维管和方法
GB201906298D0 (en) * 2019-05-03 2019-06-19 Arborea Ltd Bioreactor device and methods
CN110117527A (zh) * 2019-05-15 2019-08-13 刘宝全 一种干细胞代谢废物的强化排出方法
AU2020387177B2 (en) 2019-11-22 2026-03-12 Cutiss Ag Methods and systems for producing skin grafts
US11492580B2 (en) 2020-05-12 2022-11-08 Southwest Research Institute Method using a three-dimensional bioprocessor
KR20210138976A (ko) * 2020-05-13 2021-11-22 아주대학교산학협력단 중공섬유와 나노섬유로 만들어진 나노시트 복합체를 이용한 바이오리액터
TWI895441B (zh) * 2020-06-10 2025-09-01 日商武田藥品工業股份有限公司 用於病毒轉導之半自動化中空纖維系統
WO2022150482A1 (en) * 2021-01-08 2022-07-14 Shayne Guiliano Methods for culturing antibody secreting cells
GB2619893A (en) 2021-03-23 2023-12-20 Terumo Bct Inc Cell capture and expansion
US12539515B2 (en) 2021-10-28 2026-02-03 Southwest Research Institute Devices for cell separation
CN113736658B (zh) * 2021-11-03 2022-01-25 北京国卫生物科技有限公司 人脐带间充质干细胞长期大规模扩增生物反应系统
WO2023081420A1 (en) 2021-11-05 2023-05-11 Abt Holding Company Culture-expanded canine progenitor cells and related methods
US12152699B2 (en) 2022-02-28 2024-11-26 Terumo Bct, Inc. Multiple-tube pinch valve assembly
WO2023190448A1 (ja) * 2022-03-29 2023-10-05 国立大学法人東海国立大学機構 間葉系幹細胞の培養方法
USD1099116S1 (en) 2022-09-01 2025-10-21 Terumo Bct, Inc. Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device
US20240076597A1 (en) * 2022-09-02 2024-03-07 Terumo Bct, Inc. Methods for cell expansion, differentiation, and/or harvesting of natural killer cells using hollow-fiber membranes
EP4687476A1 (en) 2023-04-06 2026-02-11 IP Ventures GmbH Hollow fibres
US20250283043A1 (en) * 2024-03-11 2025-09-11 Terumo Bct, Inc. Methods for differentiation of induced pluripotent stem cells using hollow fiber bioreactors

Family Cites Families (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3821087A (en) 1972-05-18 1974-06-28 Dedrick R Cell culture on semi-permeable tubular membranes
US3883393A (en) 1972-05-18 1975-05-13 Us Health Education & Welfare Cell culture on semi-permeable tubular membranes
US3997396A (en) 1973-07-02 1976-12-14 Monsanto Company Method for the in vitro propagation and maintenance of cells
US4200689A (en) 1977-11-11 1980-04-29 United States Of America Method of cell culture using a dual circuit, woven artificial capillary bundle
US4184922A (en) 1977-11-11 1980-01-22 The Government Of The United States Dual circuit, woven artificial capillary bundle for cell culture
US4220725A (en) 1978-04-03 1980-09-02 United States Of America Capillary cell culture device
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
US4804628A (en) 1984-10-09 1989-02-14 Endotronics, Inc. Hollow fiber cell culture device and method of operation
US5192553A (en) 1987-11-12 1993-03-09 Biocyte Corporation Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of therapeutic use
US4999298A (en) 1988-04-27 1991-03-12 W. R. Grace & Co.-Conn. Hollow fiber bioreactor culture system and method
US6436701B1 (en) 1988-09-21 2002-08-20 Babraham Institute Derivation of pluripotential embryonic cell lines from ungulate species
US5162225A (en) 1989-03-17 1992-11-10 The Dow Chemical Company Growth of cells in hollow fibers in an agitated vessel
US7037721B1 (en) 1990-01-29 2006-05-02 Hy-Gene Biomedical, Inc. Protein-free defined media for the growth of normal human keratinocytes
US5656421A (en) 1990-02-15 1997-08-12 Unisyn Technologies, Inc. Multi-bioreactor hollow fiber cell propagation system and method
US5126238A (en) 1990-02-15 1992-06-30 Unisyn Fibertec Corporation Hollow fiber cell propagation system and method
US5061620A (en) 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell
US5635387A (en) 1990-04-23 1997-06-03 Cellpro, Inc. Methods and device for culturing human hematopoietic cells and their precursors
AU7954191A (en) 1990-05-30 1991-12-31 Cellco, Inc. Culturing bone marrow cells for adoptive immunotherapy
US5811094A (en) 1990-11-16 1998-09-22 Osiris Therapeutics, Inc. Connective tissue regeneration using human mesenchymal stem cell preparations
US5837539A (en) 1990-11-16 1998-11-17 Osiris Therapeutics, Inc. Monoclonal antibodies for human mesenchymal stem cells
GB2254289B (en) 1991-04-05 1995-03-22 Gerber Garment Technology Inc Readily transferrable adherent tape and methods of use and making
US5851832A (en) 1991-07-08 1998-12-22 Neurospheres, Ltd. In vitro growth and proliferation of multipotent neural stem cells and their progeny
AU2882992A (en) 1991-11-06 1993-06-07 Arthur A. Axelrad Cell culture medium
WO1993018214A1 (en) 1992-03-13 1993-09-16 Atrium Medical Corporation Controlled porosity expanded polytetrafluoroethylene products and fabrication
US5460964A (en) 1992-04-03 1995-10-24 Regents Of The University Of Minnesota Method for culturing hematopoietic cells
US5340740A (en) 1992-05-15 1994-08-23 North Carolina State University Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
WO1994000484A1 (en) 1992-06-22 1994-01-06 Young Henry E Scar inhibitory factor and use thereof
US5849553A (en) 1992-07-27 1998-12-15 California Institute Of Technology Mammalian multipotent neural stem cells
US5564183A (en) 1992-09-30 1996-10-15 Matsushita Electric Industrial Co., Ltd. Producing system of printed circuit board and method therefor
US7153684B1 (en) 1992-10-08 2006-12-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5766951A (en) 1992-11-12 1998-06-16 Quality Biological, Inc. Serum-free medium supporting growth and proliferation of normal bone marrow cells
US5872094A (en) 1993-01-06 1999-02-16 The General Hospital Corporation Method for attaching cartilaginous tissue, cartilage matrix protein or link protein to a surface
US5766948A (en) 1993-01-06 1998-06-16 The Regents Of The University Of California Method for production of neuroblasts
EP0713364A4 (en) 1993-08-13 1996-12-27 Shalaby W Shalaby MICROPOROUS POLYMERIC FOAMS AND MICROTEXTURED SURFACES
US6037174A (en) 1993-08-23 2000-03-14 Nexell Therapeutics, Inc. Preparation of serum-free suspensions of human hematopoietic cells or precursor cells
US5409813A (en) 1993-09-30 1995-04-25 Systemix, Inc. Method for mammalian cell separation from a mixture of cell populations
US6432711B1 (en) 1993-11-03 2002-08-13 Diacrin, Inc. Embryonic stem cells capable of differentiating into desired cell lines
WO1995013088A1 (en) 1993-11-12 1995-05-18 Regents Of The University Of Minnesota Stroma-derived stem cell growth factors
WO1995021911A1 (en) 1994-02-09 1995-08-17 Unisyn Technologies, Inc. High performance cell culture bioreactor and method
US5622857A (en) 1995-08-08 1997-04-22 Genespan Corporation High performance cell culture bioreactor and method
FR2726003B1 (fr) 1994-10-21 2002-10-18 Agronomique Inst Nat Rech Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
US5677136A (en) 1994-11-14 1997-10-14 Systemix, Inc. Methods of obtaining compositions enriched for hematopoietic stem cells, compositions derived therefrom and methods of use thereof
US5914268A (en) 1994-11-21 1999-06-22 National Jewish Center For Immunology & Respiratory Medicine Embryonic cell populations and methods to isolate such populations
US5874301A (en) 1994-11-21 1999-02-23 National Jewish Center For Immunology And Respiratory Medicine Embryonic cell populations and methods to isolate such populations
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5736396A (en) 1995-01-24 1998-04-07 Case Western Reserve University Lineage-directed induction of human mesenchymal stem cell differentiation
US5837670A (en) 1995-04-18 1998-11-17 Hartshorn; Richard Timothy Detergent compositions having suds suppressing properties
US5908782A (en) 1995-06-05 1999-06-01 Osiris Therapeutics, Inc. Chemically defined medium for human mesenchymal stem cells
US6015554A (en) 1995-06-07 2000-01-18 Systemix, Inc. Method of reconstituting human lymphoid and dendritic cells
US5712154A (en) 1995-06-07 1998-01-27 W.R. Grace & Co.-Conn. Dual fiber bioreactor
US6680166B1 (en) 1995-06-07 2004-01-20 Claudy Jean Paul Mullon Dual fiber bioreactor
US5627070A (en) 1995-07-26 1997-05-06 Celltherapy, Inc. Cell growing device for in vitro cell population expansion
US6190910B1 (en) 1996-05-21 2001-02-20 The Institute Of Physical And Chemical Research Mouse embryonic stem cell lines
US5753506A (en) 1996-05-23 1998-05-19 Cns Stem Cell Technology, Inc. Isolation propagation and directed differentiation of stem cells from embryonic and adult central nervous system of mammals
US5827740A (en) 1996-07-30 1998-10-27 Osiris Therapeutics, Inc. Adipogenic differentiation of human mesenchymal stem cells
US5945337A (en) 1996-10-18 1999-08-31 Quality Biological, Inc. Method for culturing CD34+ cells in a serum-free medium
GB9704749D0 (en) 1997-03-07 1997-04-23 Univ London Tissue Implant
US6361996B1 (en) 1997-05-07 2002-03-26 University Of Utah Research Foundation Neuroepithelial stem cells and glial-restricted intermediate precursors
AU7984498A (en) 1997-06-25 1999-01-04 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Serum-free cell growth medium
AU755657B2 (en) 1997-07-04 2002-12-19 University Of Utah Research Foundation Lineage-restricted neuronal precursors
US5968829A (en) 1997-09-05 1999-10-19 Cytotherapeutics, Inc. Human CNS neural stem cells
CN1280611A (zh) * 1997-09-25 2001-01-17 糖技术公司 结合造血干细胞的方法和组合物
US6001585A (en) 1997-11-14 1999-12-14 Cellex Biosciences, Inc. Micro hollow fiber bioreactor
WO1999047922A2 (en) 1998-03-18 1999-09-23 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
US6080581A (en) 1998-07-02 2000-06-27 Charles Daniel Anderson Culture vessel for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using same
JP2002529071A (ja) 1998-11-09 2002-09-10 コンソルツィオ・ペール・ラ・ジェスティオーネ・デル・チェントロ・ディ・ビオテクノロジー・アヴァンツァテ 軟骨細胞様細胞のための無血清培地
WO2000027995A1 (en) 1998-11-09 2000-05-18 Monash University Embryonic stem cells
BR0009403A (pt) 1999-02-04 2001-11-27 Technion Res & Dev Foundation Método de expansão/conservação das células detronco hemopoiéticas indiferenciadas ou dascélulas progenitoras, método de preparação deum meio condicionado de célula estomacal útil naexpansão/conservação das células de troncohemopoiéticas indiferenciadas ou das célulasprogenitoras, método de transplante de célulasde tronco hemopoiéticas indiferenciadas ou decélulas progenitoras em um recipiente, tampão debiorreator e biorreator
US20050153442A1 (en) 1999-03-10 2005-07-14 Adam Katz Adipose-derived stem cells and lattices
EP2348104A1 (en) * 1999-08-05 2011-07-27 Mcl Llc Multipotent adult stem cells and methods for isolation
US7015037B1 (en) * 1999-08-05 2006-03-21 Regents Of The University Of Minnesota Multiponent adult stem cells and methods for isolation
US8147824B2 (en) * 1999-08-05 2012-04-03 Athersys, Inc. Immunomodulatory properties of multipotent adult progenitor cells and uses thereof
US7838289B2 (en) 2001-02-14 2010-11-23 Abt Holding Company Assay utilizing multipotent adult stem cells
US6471689B1 (en) 1999-08-16 2002-10-29 Thomas Jefferson University Implantable drug delivery catheter system with capillary interface
AUPQ319199A0 (en) 1999-09-30 1999-10-28 Unisearch Limited Method and apparatus for culturing cells
WO2001040272A2 (en) 1999-12-01 2001-06-07 Selective Genetics, Inc. In situ bioreactors and methods of use thereof
WO2001098463A1 (en) 2000-06-20 2001-12-27 Es Cell International Pte Ltd Method of controlling differentiation of embryonic stem (es) cells by culturing es cells in the presence of bmp-2 pathway antagonists
US7560280B2 (en) 2000-11-03 2009-07-14 Kourion Therapeutics Gmbh Human cord blood derived unrestricted somatic stem cells (USSC)
US6566126B2 (en) 2001-06-22 2003-05-20 Fibercell Systems, Inc. Apparatus and method for growing cells
CA2458362A1 (en) 2001-08-23 2003-03-06 Reliance Life Sciences Pvt., Ltd. Isolation of inner cell mass for the establishment of human embryonic stem cell (hesc) lines
US9969980B2 (en) 2001-09-21 2018-05-15 Garnet Biotherapeutics Cell populations which co-express CD49c and CD90
US6703279B2 (en) 2002-01-04 2004-03-09 Promos Technologies, Inc. Semiconductor device having contact of Si-Ge combined with cobalt silicide
CA2473360A1 (en) 2002-01-25 2003-08-07 Genzyme Corporation Serum-free media for chondrocytes and methods of use thereof
US7145057B2 (en) 2002-02-01 2006-12-05 Origen Therapeutics, Inc. Chimeric bird from embryonic stem cells
WO2003080197A1 (en) 2002-03-20 2003-10-02 Sara Griffin Sports training device
EP1367119B1 (en) 2002-05-28 2008-09-17 Toyo Boseki Kabushiki Kaisha Methods of culturing, storing, and inducing differentiation in cells, instrument for use in the methods and method of using the instrument.
GB0221481D0 (en) 2002-09-16 2002-10-23 Richards John M Grip for hand held instruments
DE10326750B4 (de) 2003-06-13 2006-07-27 Gerlach, Jörg, Dr.med. Verfahren zur Herstellung einer Zellpräparation und derart hergestellte Zellpräparationen
DE10326748B4 (de) 2003-06-13 2006-11-23 Gerlach, Jörg, Dr.med. Nervenzellkulturbioreaktor und hybrides Nervenzellsystem
US20060205071A1 (en) 2003-07-17 2006-09-14 Gamida-Cell Ltd. Methods for ex-vivo expanding stem/progenitor cells
CN101248172A (zh) * 2005-01-28 2008-08-20 诺瓦塞拉有限公司 胚胎干细胞培养方法
US20080206733A1 (en) 2005-02-03 2008-08-28 National University Corporation Okayama University Method of Inducing Differentiation of Embryo-Stem Cell Into Hepatocyte and Hepatocyte Induced by the Method
GB2443370A (en) 2005-07-29 2008-04-30 Australian Stem Cell Ct Ltd Compositions and methods for growth of pluripotent cells
EP1940423B1 (en) 2005-09-09 2014-04-16 Duke University Tissue engineering methods and compositions
ZA200803929B (en) * 2005-10-13 2009-08-26 Anthrogenesis Corp Production of oligodendrocytes from placenta-derived stem cells
WO2007091255A2 (en) 2006-02-06 2007-08-16 Pluristem Ltd. Method and apparatus for maintenance and expansion of hematopoietic stem cells from mononuclear cells
US20070298497A1 (en) * 2006-06-26 2007-12-27 Gambro Bct, Inc. Method of Culturing Mesenchymal Stem Cells
DE102006031871B4 (de) 2006-07-10 2008-10-23 Gerlach, Jörg, Dr.med. 3-D Petri-Schale zur Züchtung und Untersuchung von Zellen
WO2008109200A1 (en) 2007-03-01 2008-09-12 Caridianbct, Inc. Disposable tubing set for use with a cell expansion apparatus and method for sterile sampling
ES2665024T3 (es) 2007-03-05 2018-04-24 Terumo Bct, Inc. Métodos para controlar el movimiento celular en biorreactores de fibra hueca
US8309347B2 (en) 2007-03-05 2012-11-13 Terumo Bct, Inc. Cell expansion system and methods of use
EP2118263A2 (en) 2007-03-14 2009-11-18 CaridianBCT, Inc. Cell expansion apparatus with plate bioreactor
KR20100016187A (ko) * 2007-04-06 2010-02-12 카리디안비씨티, 인크. 개선된 생물반응기 표면
EP2148922B1 (en) 2007-04-13 2020-03-04 Terumo BCT, Inc. Cell expansion system and methods of use
BRPI0813787A2 (pt) * 2007-07-01 2014-10-07 Lifescan Inc Cultura de célula-tronco pluripotente isolada
KR20140107677A (ko) 2007-09-19 2014-09-04 플루리스템 리미티드 지방 또는 태반 조직 유래의 부착 세포 및 이의 치료 용도
EP2245141B1 (en) * 2008-01-18 2018-03-14 Regents of the University of Minnesota Stem cell aggregates and methods for making and using
EP2321403B1 (en) 2008-08-12 2020-07-22 Terumo BCT, Inc. A predictor of when to harvest cells grown in a bioreactor
EP2168668A1 (en) 2008-09-25 2010-03-31 Gambro Lundia AB Membrane for cell expansion
US8673637B2 (en) 2008-10-16 2014-03-18 The Board Of Trustees Of The Leland Stanford Junior University Human multipotent germ line stem cells expressing a germ line marker and a pluripotency marker produced by co-culture of testicular tissue
CN102459574B (zh) * 2009-06-18 2016-06-29 塞拉提斯股份公司 用于人多潜能干(hPS)细胞的生长和分化的3D培养系统
US9550975B2 (en) * 2009-07-15 2017-01-24 Mari Dezawa SSEA-3 pluripotent stem cell isolated from body tissue
SG178064A1 (en) 2009-07-21 2012-03-29 Abt Holding Co Use of stem cells to reduce leukocyte extravasation
EP2718416B1 (en) * 2011-06-06 2019-11-13 Regenesys bvba Expansion of stem cells in hollow fiber bioreactors

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