DK2836587T3 - Biobeskyttelse under anvendelse af Lactobacillus rhamnosus-stammer - Google Patents

Biobeskyttelse under anvendelse af Lactobacillus rhamnosus-stammer Download PDF

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DK2836587T3
DK2836587T3 DK13714671.8T DK13714671T DK2836587T3 DK 2836587 T3 DK2836587 T3 DK 2836587T3 DK 13714671 T DK13714671 T DK 13714671T DK 2836587 T3 DK2836587 T3 DK 2836587T3
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strain
product
lactobacillus rhamnosus
lactobacillus
cfu
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DK13714671.8T
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English (en)
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Maike Lisberg
Silja Kej Diemer
Tina Hornbaek
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Chr Hansen As
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Priority claimed from PCT/EP2012/056384 external-priority patent/WO2012136830A1/en
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Publication of DK2836587T3 publication Critical patent/DK2836587T3/da

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
    • A23B2/729—Organic compounds; Microorganisms; Enzymes
    • A23B2/783—Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B4/00—Preservation of meat, sausages, fish or fish products
    • A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
    • A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
    • A23B4/20—Organic compounds; Microorganisms; Enzymes
    • A23B4/22—Microorganisms; Enzymes; Antibiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C13/00—Cream; Cream preparations; Making thereof
    • A23C13/12—Cream preparations
    • A23C13/16—Cream preparations containing, or treated with, microorganisms, enzymes, or antibiotics; Sour cream
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0323—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin using only lactic acid bacteria, e.g. Pediococcus and Leuconostoc species; Bifidobacteria; Microbial starters in general
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A23C19/076—Soft unripened cheese, e.g. cottage or cream cheese
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/158—Milk preparations; Milk powder or milk powder preparations containing additives containing vitamins or antibiotics
    • A23C9/1585—Antibiotics; Bacteriocins; Fungicides from microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/165—Paracasei
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/175—Rhamnosus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/853—Lactobacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Husbandry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Physiology (AREA)

Claims (15)

1. Lactobacillus rhamnosus-stamme, som er valgt fra gruppen bestående af Lactobacillus rhamnosus-stammen CHCC5366, som blev deponeret hos German Collection of Microorganisms and Cell Cultures (DSMZ) under deponeringsnummer DSM23035, og mutantstammer afledt deraf, hvor mutantstammerne har i det væsentlige de samme eller forbedrede antifungale egenskaber i forhold til den stamme, der er deponeret under deponeringsnummer DSM23035.
2. Antifungalt præparat, som omfatter mindst én Lactobacillus rhamnosus-stamme ifølge krav 1.
3. Antifungalt præparat ifølge krav 2, som desuden omfatter mindst én stamme af Lactobacillus paracasei.
4. Antifungalt præparat ifølge krav 3, hvor den mindst ene stamme af Lactobacillus paracasei er valgt fra gruppen bestående af Lactobacillus paracasei CHCC12777 med deponeringsnummer DSM24651 og Lactobacillus paracasei CHCC14676 med deponeringsnummer DSM25612 og mutantstammer afledt deraf, hvor mutantstammerne har i det væsentlige de samme eller forbedrede antifungale egenskaber i forhold til én af de stammer, der er deponeret under deponeringsnummer DSM24651 eller DSM25612.
5. Fødevareprodukt, foderprodukt eller farmaceutisk produkt, som omfatter en Lactobacillus rhamnosus-stamme ifølge krav 1 eller et antifungalt præparat ifølge et hvilket som helst af kravene 2 til 4.
6. Fødevareprodukt ifølge krav 5, som omfatter en mængde af en Lactobacillus rham-nosus-stamme ifølge krav 1, som er effektiv til at bibringe fødevareproduktet antifungale egenskaber.
7. Fødevareprodukt ifølge krav 5 eller 6, hvor fødevareproduktet er valgt fra gruppen bestående af frugter og frugtafledte produkter, grønsager og grønsagsafledte produkter, kom og kornafledte produkter, mejeriprodukter, kød, fjerkræ samt fisk og skaldyr samt blandinger deraf.
8. Fødevareprodukt ifølge krav 7, hvor fødevareproduktet er et mejeriprodukt, og mejeriproduktet er et mesofilt eller et termofilt fermenteret mælkeprodukt, såsom yoghurt.
9. Fremgangsmåde til fremstilling af et fødevareprodukt, foderprodukt eller farmaceutisk produkt, hvilken fremgangsmåde omfatter tilsætning af mindst én Lactobacillus rhamnosus-stamme ifølge krav 1 eller et antifungalt præparat ifølge et hvilket som helst af kravene 2 til 4 til fødevareproduktet, foderproduktet eller det farmaceutiske produkt under fremstilling og regulering af fremstillingsparametrene under fremstillingen således, at koncentrationen af den mindst ene Lactobacillus rhamnosus-stamme forbliver konstant eller øges.
10. Fremgangsmåde ifølge krav 9, hvor koncentrationen af den mindst ene Lactobacillus rhamnosus-stamme er mindst 1x106 cfu/g eller hver mindst 1x106 cfu/ml af fødevareproduktet, foderproduktet eller det farmaceutiske produkt eller mindst 1x105 cfu/cm2 af overfladen af fødevareproduktet, foderproduktet eller det farmaceutiske produkt.
11. Fremgangsmåde ifølge krav 9, som omfatter (a) tilsætning af det antifungale præparat ifølge et hvilket som helst af kravene 2 til 4 under fremstillingen af fødevareproduktet, foderproduktet eller det farmaceutiske produkt, således at koncentrationen af den mindst ene stamme af Lactobacillus rhamnosus og/eller den mindst ene stamme af Lactobacillus paracasei hver er mindst 1x106 cfu/g eller mindst 1x106 cfu/ml af fødevareproduktet, foderproduktet eller det farmaceutiske produkt eller mindst 1x105 cfu/cm2 af overfladen af fødevareproduktet, foderproduktet eller det farmaceutiske produkt, og (b) regulering af fremstillingsparametrene under fremstillingen, således at koncentrationen af den mindst ene stamme af Lactobacillus rhamnosus og/eller den mindst ene stamme af Lactobacillus paracasei øges eller forbliver konstant.
12. Fremgangsmåde ifølge et hvilket som helst af kravene 9 til 11, hvor fremgangsmåden omfatter ét eller flere fermenteringstrin.
13. Fremgangsmåde ifølge krav 12, hvor fremgangsmåden omfatter fermentering af et mælkesubstrat med en starterkultur, som omfatter mindst én stamme af de slægter, der er valgt blandt Lactobacillus, Streptococcus, Lactococcus og Leuconostoc.
14. Anvendelse af en Lactobacillus rhamnosus-stamme ifølge krav 1 eller et antifungalt præparat ifølge et hvilket som helst af kravene 2 til 4 til fremstilling af et fødevareprodukt, foderprodukt eller farmaceutisk produkt.
15. Ikke-medicinsk anvendelse af en Lactobacillus rhamnosus-stamme ifølge krav 1 eller et antifungalt præparat ifølge et hvilket som helst af kravene 2 til 4 til inhibering af væksten af gær og skimmelsvampe.
DK13714671.8T 2012-04-09 2013-04-09 Biobeskyttelse under anvendelse af Lactobacillus rhamnosus-stammer DK2836587T3 (da)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
PCT/EP2012/056384 WO2012136830A1 (en) 2011-04-08 2012-04-09 Synergistic antimicrobial effect
EP12163508 2012-04-09
EP12168977 2012-05-22
EP13154591 2013-02-08
PCT/EP2013/057410 WO2013153074A1 (en) 2012-04-09 2013-04-09 Bioprotection using lactobacillus rhamnosus strains

Publications (1)

Publication Number Publication Date
DK2836587T3 true DK2836587T3 (da) 2017-05-08

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DK13714671.8T DK2836587T3 (da) 2012-04-09 2013-04-09 Biobeskyttelse under anvendelse af Lactobacillus rhamnosus-stammer

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US (1) US9485992B2 (da)
EP (1) EP2836587B1 (da)
JP (1) JP6430931B2 (da)
KR (1) KR101947110B1 (da)
CN (1) CN104284975B (da)
AR (1) AR090631A1 (da)
BR (1) BR112014024918B1 (da)
DK (1) DK2836587T3 (da)
EA (2) EA033656B1 (da)
ES (1) ES2630711T3 (da)
HK (1) HK1206391A1 (da)
HU (1) HUE034259T2 (da)
MX (1) MX351628B (da)
PL (1) PL2836587T3 (da)
PT (1) PT2836587T (da)
UA (1) UA115330C2 (da)
WO (1) WO2013153074A1 (da)

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EA028377B1 (ru) 2011-04-08 2017-11-30 Кр. Хансен А/С Противомикробная композиция
MX351628B (es) 2012-04-09 2017-10-23 Chr Hansen As Bioproteccion usando cepas de lactobacillus rhamnosus.
DK2836588T3 (da) 2012-04-09 2017-05-22 Chr Hansen As Biobeskyttelse under anvendelse af Lactobacillus paracasei-stammer
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CN107949633B (zh) * 2015-08-12 2021-04-30 Cj第一制糖株式会社 鼠李糖乳杆菌、动物饲料及其组合物及无活性细胞生产法
KR20180042408A (ko) * 2015-08-31 2018-04-25 시에이치알. 한센 에이/에스 아세트알데히드의 농도를 감소시키는 락토바실러스 퍼멘텀 박테리아
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DK3279312T3 (da) 2016-08-02 2019-10-28 Dsm Ip Assets Bv Biobeskyttelse af mejeriprodukter
PL3700344T3 (pl) * 2017-10-27 2022-01-17 Chr. Hansen A/S Lactobacillus rhamnosus o zwiększonym wytwarzaniu diacetylu
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US11707070B2 (en) * 2018-04-19 2023-07-25 Chr. Hansen A/S Inhibition of fungal growth by manganese depletion
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FR3105258B1 (fr) 2019-12-23 2022-10-14 Flores de Terroirs Levains bioprotecteurs obtenus à partir de laits natifs : utilisation de ces levains pour préparer des levains terroirs et pour purifier le lait cru
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