DK2872641T3 - Bioraffinaderisystem, fremgangsmåder og sammensætninger deraf - Google Patents

Bioraffinaderisystem, fremgangsmåder og sammensætninger deraf Download PDF

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DK2872641T3
DK2872641T3 DK13817140.0T DK13817140T DK2872641T3 DK 2872641 T3 DK2872641 T3 DK 2872641T3 DK 13817140 T DK13817140 T DK 13817140T DK 2872641 T3 DK2872641 T3 DK 2872641T3
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metabolizing
microorganism
oil composition
recombinant
methane
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Joshua Silverman
Sol M Resnick
Michael Mendez
Renee Saville
Sungwon Lee
Luan Nguyen
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Calysta Inc
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Claims (15)

1. Rekombinant Ci metaboliserende ikke-fotosyntetisk mikroorganisme, hvor den rekombinante Ci metaboliserende ikke-fotosyntetisk mikroorganisme omfatter et heterologt polynukleotid der koder for en thioesterase, en malonyl CoA-acylbærer-proteintransacylase, en acetyl-CoA-carboxylase eller en hvilken som helst kombination deraf, og hvor ekspression af det heterologe polynukleotid fører til akkumulering af et forhøjet niveau af fedtsyrer i den rekombinante Ci metaboliserende ikke-fotosyntetisk mikroorganisme sammenlignet med den forælder-Ci metaboliserende ikke-fotosyntetiske mikroorganisme, hvor den Ci metaboliserende ikke-fotosyntetiske mikroorganisme er en methylotroph og/eller methanotroph bakterie.
2. Rekombinant Ci metaboliserende ikke-fotosyntetisk mikroorganisme ifølge krav 1, hvor: (i) thioesterasen er et kodon optimeret for den Ci metaboliserende ikke-fotosyntetiske mikroorganisme; (ii) malonyl CoA-acylbærerproteintransacylasen er et kodon optimeret E. coli fabD) (iii) acetyl-CoA carboxylasen er et kodon optimeret E. coli accA, accB, accC, accD, eller en hvilken som helst kombination deraf; eller (iv) rekombinant Ci metaboliserende mikroorganisme yderligere omfattende en mutation der minimerer eller elimineret fedtsyre-CoA ligaseaktivitet.
3. Rekombinant Ci metaboliserende ikke-fotosyntetisk mikroorganisme ifølge et hvilket som helst af de foregående krav, hvor den rekombinante Ci metaboliserende ikke-fotosyntetiske mikroorganisme er en methylotroph bakterie.
4. Rekombinant Ci metaboliserende ikke-fotosyntetisk mikroorganisme ifølge krav 3, hvor forælder-bakterien er en Methylobacterium extorquens, Methylobacterium radiotolerans, Methylobacterium populi, Methylobacterium chloromethanicum, eller Methylobacterium nodularis.
5. Rekombinant Ci metaboliserende ikke-fotosyntetisk mikroorganisme ifølge et hvilket som helst af kravene 1 og 2, hvor den rekombinante Ci metaboliserende ikke-fotosyntetiske mikroorganisme er en methanotroph bakterie.
6. Rekombinant Ci metaboliserende ikke-fotosyntetisk mikroorganisme ifølge krav 5, hvor forælder-bakterien er Methylomonas sp. 16a (ATCC PTA 2402), Methylosinus trichosporium OB3b (NRRL B-11,196), Methylosinus sporium (NRRL B-11,197), Methylocystisparvus (NRRL B-11,198), Methylomonas methanica (NRRL B-11,199), Methylomonas albus (NRRL B-11,200), Methylobacter capsulatus Y (NRRL B-11,201), Methylococcus capsulatus Bath (NCIMB 11132), Methylobacterium organophilum (ATCC 27,886), Methylomonas sp. AJ-3670 (FERM P-2400), Methylomicrobium alcaliphilum, Methylocella silvestris, Methylacidiphilum infernorum, eller Methylibium petroleiphilum,.
7. Styret dyrkningsenhed i hvilken et Ci substrat, eventuelt methan, leveres i en gasfase til en mikrobiel biofilm i en fastfase fermentation, og hvor den mikrobielle biofilm omfatter en rekombinant Ci metaboliserende mikroorganisme ifølge et hvilket som helst af kravene 1-6.
8. Fremgangsmåde til fremstilling afen oliesammensætning, hvilken fremgangsmåde omfatter: (i) dyrke en mikroorganisme ifølge et hvilket som helst af kravene 1-6 på et Ci substrat ved batchdyrkning eller kontinuerlig dyrkning; og (ii) ekstrahere oliesammensætningen fra cellemembranen af mikroorganismen eller genvinding af oliesammensætningen fra dyrkningssupernatanten, eller en kombination deraf.
9. Fremgangsmåden ifølge krav 8, hvor oliesammensætningen omfatter molekyler omfattende hydrogen- og carbonatomer, hvor hydrogen- og carbonatomerne er mindst 50 vægtprocent af sammensætningen og hvor 513C af oliesammensætningen ligger i området fra ca. -70%o til -30%o.
10. Fremgangsmåden ifølge krav 8, hvor oliesammensætningen omfatter mere end 50% vægt/vægt terpenoid- eller isoprenoidforbindelser.
11. Fremgangsmåden ifølge krav 10, hvor terpenoidet erfarnesen, limonen, eller begge.
12. Fremgangsmåden ifølge krav 8, yderligere omfattende raffinering af oliesammensætningen ved hydrobehandling for at fremstille kerosin, diesel, eller en kombination deraf, hvor hydrobehandlingen producerer lette carbonhydrider valgt fra methan, ethan, propan, butan, pentan, butanol og isobutanol.
13. Fremgangsmåden ifølge et hvilket som helst af kravene 8-12, hvor bakterien er en Methylomonas sp. 16a (ATCC PTA 2402), Methylosinus trichosporium OB3b (NRRL B-11,196), Methylosinus sporium (NRRL B-11,197), Methylocystis parvus (NRRL B-11,198), Methylomonas methanica (NRRL B-11,199), Methylomonas albus (NRRL B-11,200), Methylobacter capsulatus Y (NRRL B-11,201), Methylococcus capsulatus Bath (NCIMB 11132), Methylobacterium organophilum (ATCC 27,886), Methylomonas sp. AJ-3670 (FERM P-2400), Methylomicrobium alcaliphilum, Methylocella silvestris, Methylacidiphilum infernorum, Methylibium petroleiphilum, Methylobacterium extorquens, Methylobacterium radiotolerans, Methylobacterium populi, Methylobacterium chloromethanicum, Methylobacterium nodulans, eller en hvilken som helst kombination deraf.
14. Fremgangsmåden ifølge et hvilket som helst af kravene 8-13, hvor Ci-substratet er naturgas, methan, eller methanol.
15. Fremgangsmåde til fremstilling af brændstof ved at omdanne biomasse fra en kultur primært omfattende em mikroorganisme ifølge et hvilket som helst af kravene 1-6 til en oliesammensætning og raffinering af oliesammensætningen til et brændstof.
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RU2014151417A (ru) 2016-08-27
EP2872641B1 (en) 2018-03-28
EP2872641A4 (en) 2015-12-30
US9410168B2 (en) 2016-08-09
US20160040198A1 (en) 2016-02-11
AU2013289943B2 (en) 2017-06-08
US20150218508A1 (en) 2015-08-06
CN104685060B (zh) 2019-11-01
US20180251800A1 (en) 2018-09-06
US20160289714A1 (en) 2016-10-06
WO2014012055A1 (en) 2014-01-16
CA2876509A1 (en) 2014-01-16
RU2723620C2 (ru) 2020-06-16
BR112015000671A2 (pt) 2017-06-27

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