DK2940120T3 - Indretning og fremgangsmåde til storvolumentransfektion - Google Patents
Indretning og fremgangsmåde til storvolumentransfektion Download PDFInfo
- Publication number
- DK2940120T3 DK2940120T3 DK14166918.4T DK14166918T DK2940120T3 DK 2940120 T3 DK2940120 T3 DK 2940120T3 DK 14166918 T DK14166918 T DK 14166918T DK 2940120 T3 DK2940120 T3 DK 2940120T3
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- Denmark
- Prior art keywords
- chamber
- suspension
- separating element
- electrode
- aliquot
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- 238000001890 transfection Methods 0.000 title claims 2
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/02—Details
- A61N1/04—Electrodes
- A61N1/0404—Electrodes for external use
- A61N1/0408—Use-related aspects
- A61N1/0412—Specially adapted for transcutaneous electroporation, e.g. including drug reservoirs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Electromagnetism (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Radiology & Medical Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Claims (14)
- Indretning og fremgangsmåde til storvolumentransfektion1. Indretning (1) til påføring af et elektrisk felt på en suspension af celler, cellederivater, organeller, subcellulære partikler og/eller vesikler, hvilken indretning omfatter mindst ét kammer (6), der omfatter mindst to elektroder (4, 5), og mindst ét separeringselement (13), som kan bevæges inde i kammeret (6) mellem to terminalpunkter (14, 15), og som er et forseglingselement, der, hvis det er i en position mellem terminalpunkterne (14, 15), sikrer væsketæt og/eller gastæt separering af mindst ét første rum (26) i kammeret (6) fra mindst ét andet rum (27) i kammeret (6), hvor det første rum (26) er udformet til at rumme en alikvot af suspensionen og omfatter mindst én åbning (7, 8) til indføring eller udledning af suspensionen ind i eller ud af det første rum (26), kendetegnet ved, at separeringselementet (13) omfatter et fleksibelt og/eller elastisk materiale, og det andet rum (27) er udformet til at rumme en yderligere alikvot af suspensionen og omfatter mindst én åbning (10, 11) til indføring eller udledning af suspensionen ind i eller ud af det andet rum (27).
- 2. Indretning ifølge krav 1, hvor mindst én åbning (7, 8) er anbragt i den ene ende (9) af kammeret (6), og mindst én yderligere åbning (10, 11) er anbragt i den modsatte ende (12) af kammeret (6).
- 3. Indretning ifølge krav 1 eller 2, hvor separeringselementet (13) er koblet til mindst ét justeringselement (19), der er anbragt uden for kammeret (6).
- 4. Indretning ifølge et hvilket som helst af kravene 1 til 3, hvor separeringselementet (13) omfatter mindst to med afstand placerede dele (16, 17), hvor det indre rum (18) mellem de med afstand placerede dele (16, 17) i separeringselementet (13) omfatter et kompressibelt materiale.
- 5. Indretning ifølge et hvilket som helst af kravene 1 til 4, hvor kammeret (6) omfatter mindst to segmenter, hvor hvert segment omfatter mindst én elektrode (4, 5).
- 6. Indretning ifølge krav 5, hvor hvert segment er forsynet med mindst én første elektrode (4) og mindst én anden elektrode (5), hvor den anden elektrode (5) er en fælles elektrode for mindst to segmenter.
- 7. Indretning ifølge et hvilket som helst af kravene 1 til 6, hvor kammeret (6) omfatter tilsvarende fordybninger (3) i to komponenter, der er fastgjort til hinanden.
- 8. Indretning ifølge krav 7, hvor hver fordybning (3) er forsynet med mindst én elektrode (4, 5).
- 9. Indretning ifølge et hvilket som helst af kravene 1 til 8, hvor kammeret (6) endvidere omfatter mindst ét basiselement (2, 30), der mindst i det væsentlige er fremstillet af et isolerende materiale og indbefatter mindst én overflade, til hvilken elektroden (4, 5) er fastgjort, hvor overfladen omfatter mindst ét ledende område (32), som er udformet til at tilvejebringe en elektrisk forbindelse mellem elektroden (4, 5) og mindst ét elektrisk kontaktpunkt (33).
- 10. Indretning ifølge et hvilket som helst af kravene 3 til 9, hvor der er anbragt mindst én pakning (22) mellem justeringselementet (19) og kammeret (6).
- 11. Indretning ifølge et hvilket som helst af kravene 1 til 10, hvor kammeret (6) omfatter mindst ét forseglingsindlæg (24), der mindst delvis strækker sig langs én side af kammeret (6), hvor forseglingsindlægget (24) omfatter et kompressibelt materiale.
- 12. Fremgangsmåde til påføring af et elektrisk felt på en suspension af celler, cellederivater, organeller, subcellulære partikler og/eller vesikler, hvilken fremgangsmåde omfatter: a) indføring af en alikvot af suspensionen i mindst ét kammer i en indretning til påføring af et elektrisk felt på suspensionen, hvilket kammer omfatter mindst to elektroder, hvor et separeringselement, der er anbragt inde i kammeret, flyttes i en første retning; b) påføring af en spændingspuls på alikvoten via mindst to elektroder i kammeret; c) udledning af alikvoten fra kammeret og samtidig indføring afen yderligere alikvot af suspensionen i kammeret, hvor separeringselementet flyttes i en anden retning modsat den første retning, og hvor separeringselementet omfatter et fleksibelt og/eller elastisk materiale og er et forseglingselement, som sikrer væsketæt og/eller gastæt separering af alikvoterne fra hinanden; d) påføring af en spændingspuls på den yderligere alikvot via de mindst to elektroder i kammeret.
- 13. Fremgangsmåde ifølge krav 12, der endvidere omfatter: e) udledning af den yderligere alikvot fra kammeret og samtidig indføring af en yderligere alikvot af suspensionen i kammeret, hvor separeringselementet flyttes i den første retning modsat den anden retning, og hvor separeringselementet separerer alikvoterne fra hinanden; f) påføring af en spændingspuls på den yderligere alikvot via mindst to elektroder i kammeret; g) valgfri gentagelse af trinnene c) til f) for yderligere alikvoter af suspensionen, indtil hele suspensionen er processeret.
- 14. Fremgangsmåde ifølge krav 12 eller 13, hvor separeringselementet flyttes ved at rotere et justeringselement, der er operativt koblet med separeringselementet.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14166918.4A EP2940120B1 (en) | 2014-05-02 | 2014-05-02 | Device and method for large volume transfection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2940120T3 true DK2940120T3 (da) | 2018-11-26 |
Family
ID=50721562
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17184306.3T DK3260163T3 (da) | 2014-05-02 | 2014-05-02 | Anordning og fremgangsmåde til transfektion af stort volumen |
| DK14166918.4T DK2940120T3 (da) | 2014-05-02 | 2014-05-02 | Indretning og fremgangsmåde til storvolumentransfektion |
| DK14191272.5T DK2940121T3 (da) | 2014-05-02 | 2014-10-31 | Anordning og fremgangsmåde til transfektion med stor volumen |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17184306.3T DK3260163T3 (da) | 2014-05-02 | 2014-05-02 | Anordning og fremgangsmåde til transfektion af stort volumen |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14191272.5T DK2940121T3 (da) | 2014-05-02 | 2014-10-31 | Anordning og fremgangsmåde til transfektion med stor volumen |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US10633646B2 (da) |
| EP (3) | EP2940120B1 (da) |
| JP (3) | JP2017514475A (da) |
| KR (3) | KR102407937B1 (da) |
| CN (3) | CN106255746B (da) |
| AU (3) | AU2015254739B2 (da) |
| CA (2) | CA2946472C (da) |
| DK (3) | DK3260163T3 (da) |
| ES (3) | ES2751732T3 (da) |
| WO (2) | WO2015165879A1 (da) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2751732T3 (es) | 2014-05-02 | 2020-04-01 | Lonza Cologne Gmbh | Dispositivo y método para la transfección de grandes volúmenes |
| CA3100050A1 (en) | 2018-05-11 | 2019-11-14 | Lupagen, Inc. | Systems and methods for closed loop, real-time modifications of patient cells |
| EP3750983B1 (en) * | 2019-06-13 | 2022-02-23 | Lonza Cologne GmbH | Method and device for controlling the filling level in a chamber |
| USD965170S1 (en) | 2020-10-23 | 2022-09-27 | Life Technologies Corporation | Electroporation device |
| JP7582746B2 (ja) * | 2021-01-26 | 2024-11-13 | 東京エレクトロン株式会社 | 制御方法及び基板搬送モジュール |
| EP4163380A1 (en) * | 2021-10-08 | 2023-04-12 | ETH Zurich | Device and method for manipulation of extracellular vesicles |
| KR20240006945A (ko) | 2022-07-07 | 2024-01-16 | 주식회사 엘지에너지솔루션 | 배터리 모듈 부품 |
| CN117431158A (zh) * | 2022-07-14 | 2024-01-23 | 苏州壹达生物科技有限公司 | 一种流式电穿孔装置 |
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| US3148624A (en) * | 1961-06-21 | 1964-09-15 | Alan W Baldwin | Hydraulic pump |
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2014
- 2014-05-02 ES ES17184306T patent/ES2751732T3/es active Active
- 2014-05-02 DK DK17184306.3T patent/DK3260163T3/da active
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