DK3041949T3 - Celler, der producerer fc-holdige molekyler med modificerede glycosyleringsmønstre, og fremgangsmåder og anvendelser deraf - Google Patents

Celler, der producerer fc-holdige molekyler med modificerede glycosyleringsmønstre, og fremgangsmåder og anvendelser deraf Download PDF

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Publication number
DK3041949T3
DK3041949T3 DK14761351.7T DK14761351T DK3041949T3 DK 3041949 T3 DK3041949 T3 DK 3041949T3 DK 14761351 T DK14761351 T DK 14761351T DK 3041949 T3 DK3041949 T3 DK 3041949T3
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DK
Denmark
Prior art keywords
procedures
cells producing
containing molecules
glycosylation patterns
modified glycosylation
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Application number
DK14761351.7T
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English (en)
Inventor
Nico Callewaert
Francis Santens
Original Assignee
Vib Vzw
Univ Gent
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Publication date
Application filed by Vib Vzw, Univ Gent filed Critical Vib Vzw
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Publication of DK3041949T3 publication Critical patent/DK3041949T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/395Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01096Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/40Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41Glycosylation, sialylation, or fucosylation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/70Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/71Decreased effector function due to an Fc-modification
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/70Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/732Antibody-dependent cellular cytotoxicity [ADCC]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/90Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94Stability, e.g. half-life, pH, temperature or enzyme-resistance
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/30Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK14761351.7T 2013-09-05 2014-09-05 Celler, der producerer fc-holdige molekyler med modificerede glycosyleringsmønstre, og fremgangsmåder og anvendelser deraf DK3041949T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13183124 2013-09-05
PCT/EP2014/068946 WO2015032899A1 (en) 2013-09-05 2014-09-05 Cells producing fc containing molecules having altered glycosylation patterns and methods and use thereof

Publications (1)

Publication Number Publication Date
DK3041949T3 true DK3041949T3 (da) 2021-07-26

Family

ID=49165526

Family Applications (1)

Application Number Title Priority Date Filing Date
DK14761351.7T DK3041949T3 (da) 2013-09-05 2014-09-05 Celler, der producerer fc-holdige molekyler med modificerede glycosyleringsmønstre, og fremgangsmåder og anvendelser deraf

Country Status (8)

Country Link
US (2) US10202590B2 (da)
EP (1) EP3041949B1 (da)
JP (1) JP6553042B2 (da)
KR (1) KR102232348B1 (da)
AU (1) AU2014317092B2 (da)
CA (1) CA2922888C (da)
DK (1) DK3041949T3 (da)
WO (1) WO2015032899A1 (da)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10202590B2 (en) 2013-09-05 2019-02-12 Vib Vzw Cells producing Fc-containing molecules having altered glycosylation patterns and methods and use thereof
CA2950423A1 (en) 2014-05-27 2015-12-03 Academia Sinica Compositions and methods relating to universal glycoforms for enhanced antibody efficacy
KR20170003720A (ko) 2014-05-27 2017-01-09 아카데미아 시니카 항-cd20 글리코항체 및 이의 용도
WO2017005925A1 (en) * 2015-07-09 2017-01-12 Vib Vzw Cells producing glycoproteins having altered n- and o-glycosylation patterns and methods and use thereof
JOP20190100A1 (ar) * 2016-11-19 2019-05-01 Potenza Therapeutics Inc بروتينات ربط مولد ضد مضاد لـ gitr وطرق استخدامها
CN108204958A (zh) * 2016-12-19 2018-06-26 伊缪泰普有限公司 结合测定
WO2018126092A1 (en) 2016-12-29 2018-07-05 Development Center For Biotechnology Processes for preparing glycoprotein-drug conjugates
WO2018206734A1 (en) 2017-05-11 2018-11-15 Vib Vzw Glycosylation of variable immunoglobulin domains
GB201714765D0 (en) 2017-09-14 2017-11-01 Vib Vzw Means and methods for the production of glycoproteins comprising homogeneous sialylated carbonhydrates
EP3682017A2 (en) * 2017-09-14 2020-07-22 Vib Vzw Genetically engineered eukaryotic cells producing lacnac-glycoproteins
GB201901608D0 (en) 2019-02-06 2019-03-27 Vib Vzw Vaccine adjuvant conjugates
GB201918279D0 (en) 2019-12-12 2020-01-29 Vib Vzw Glycosylated single chain immunoglobulin domains
PE20240762A1 (es) * 2021-03-12 2024-04-17 Univ Rockefeller Nanocuerpos especificos de glicoformas y metodos de uso
WO2024251826A1 (en) 2023-06-05 2024-12-12 Vib Vzw Glyco-engineered antibody-drug-conjugates comprising an oxime linker
WO2025221083A1 (ko) * 2024-04-19 2025-10-23 피티 칼베 제넥신 바이올로직스 지속형 epo-fc 융합 단백질 생산 방법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69228665T2 (de) 1991-12-23 1999-07-22 Dsm N.V., Heerlen Eukaryotisches Expressionssystem
US5721121A (en) 1995-06-06 1998-02-24 Genentech, Inc. Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein
EP2322644A1 (en) 2000-06-28 2011-05-18 GlycoFi, Inc. Methods for producing modified glycoproteins
DE10062302A1 (de) 2000-12-14 2002-07-11 Aventis Res & Tech Gmbh & Co Sekretionssignalpeptide, deren DNA-Sequenzen, damit herstellbare Expressionsvektoren für eukaryotische Zellen und deren Verwendung zur biotechnologischen Herstellung von Proteinen
WO2008120107A2 (en) 2007-04-03 2008-10-09 Oxyrane (Uk) Limited Glycosylation of molecules
CA2733362C (en) * 2008-08-08 2016-10-18 Universiteit Gent Cells producing glycoproteins having altered glycosylation patterns and methods and use thereof
US10087236B2 (en) 2009-12-02 2018-10-02 Academia Sinica Methods for modifying human antibodies by glycan engineering
CA2862925C (en) 2012-02-10 2020-01-21 University Of Maryland, Baltimore Chemoenzymatic glycoengineering of antibodies and fc fragments thereof
US10202590B2 (en) 2013-09-05 2019-02-12 Vib Vzw Cells producing Fc-containing molecules having altered glycosylation patterns and methods and use thereof

Also Published As

Publication number Publication date
JP6553042B2 (ja) 2019-07-31
KR102232348B1 (ko) 2021-03-29
AU2014317092B2 (en) 2018-02-08
AU2014317092A1 (en) 2016-03-24
US20190024066A1 (en) 2019-01-24
US20160200825A1 (en) 2016-07-14
CA2922888A1 (en) 2015-03-12
WO2015032899A1 (en) 2015-03-12
JP2016533757A (ja) 2016-11-04
US11421209B2 (en) 2022-08-23
EP3041949B1 (en) 2021-04-28
EP3041949A1 (en) 2016-07-13
KR20160048213A (ko) 2016-05-03
CA2922888C (en) 2023-04-11
US10202590B2 (en) 2019-02-12

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