DK3092491T3 - Proteiner, som er målrettede orthologer - Google Patents
Proteiner, som er målrettede orthologer Download PDFInfo
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- DK3092491T3 DK3092491T3 DK15734951.5T DK15734951T DK3092491T3 DK 3092491 T3 DK3092491 T3 DK 3092491T3 DK 15734951 T DK15734951 T DK 15734951T DK 3092491 T3 DK3092491 T3 DK 3092491T3
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Classifications
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
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Claims (15)
1. Fremgangsmåde til identifikation af et flere arter omfattende mutant polypeptid ud fra et modelpolypeptid fra en art valgt blandt menneske og ikke-menneske-dyr, hvor modelpolypeptidet udviser en aktivitet på et mål fra arten og har totalt n aminosyrerester, hvilken fremgangsmåde omfatter følgende trin: i. generering af et bibliotek af mutante polypeptider i en eukaryotisk cellevært ud fra modelpolypeptidet ved at generere n-1 separate sæt af mutante polypeptider ud fra modelpolypeptidet, idet hvert sæt omfatter mutante polypeptider med X forskellige forudbestemte aminosyrerester ved en enkelt forudbestemt position i modelpolypeptidet, idet hvert sæt af mutante polypeptider afviger i den enkelte forudbestemte position, og et antal forskellige mutante polypeptider genereret er ækvivalent med [n-1] x X; ii. udvælgelse af mutante polypeptider fra biblioteket af mutante polypeptider i den eukaryotiske cellevært, som udviser aktiviteten på en ortholog af målet i en anden art valgt blandt menneske og ikke-menneske-dyr; og iii. screening af de valgte mutante polypeptider i den eukaryotiske cellevært for et flere arter omfattende mutant polypeptid, som udviser aktiviteten på målet.
2. Fremgangsmåde ifølge krav 1, hvor modelpolypeptidet er et antistof og målet er et antigen, som antistoffet specifikt binder til.
3. Fremgangsmåde ifølge krav 1, hvor modelpolypeptidet er et enzym og målet er et substrat af enzymet.
4. Fremgangsmåde ifølge krav 1, hvor modelpolypeptidet er et hormon og målet er en receptor for hormonet.
5. Fremgangsmåde ifølge krav 1, hvor genereringstrinnet omfatter anvendelse af comprehensive positional evolution (CPE).
6. Fremgangsmåde ifølge krav 1, hvor n er 19 og det totale antal af forskellige polypeptider, som genereres, er ækvivalent med [n-1] x 19.
7. Fremgangsmåde ifølge krav 1, hvor hvert sæt af de mutante polypeptider genereres ved: inkorporering af Ν,Ν,Ν ved en forudbestemt codonposition i et polynukleotid, som koder for modelpolypeptidet.
8. Fremgangsmåde ifølge krav 1, hvor arten er menneske.
9. Fremgangsmåde ifølge krav 8, hvor den anden art er valgt blandt rotte, mus, kanin, hamster, marsvin, abe, hund, kat og ikke-menneske-primater.
10. Fremgangsmåde ifølge krav 1, hvor arten og den anden art er forskellige og uafhængigt valgt blandt rotte, mus, kanin, hamster, marsvin, abe, hund, kat og ikke-menneske-primater.
11. Fremgangsmåde ifølge krav 1, hvor den eukaryotiske cellevært er valgt blandt en gærcellevært og en mammal cellevært.
12. Fremgangsmåde ifølge krav 11, hvor den eukaryotiske cellevært er den mammale cellevært.
13. Fremgangsmåde ifølge krav 12, hvor den mammale cellevært er valgt blandt CHO, HEK293, IM9, DS-1, THP-1, Hep G2, COS, NIH 3T3, C33a, A549, A375, SK-MEL-28, DU 145, PC-3, HCT 116, Mia PACA-2, ACHN, Jurkat, MM1, Ovcar 3, HT 1080, Panc-1, U266, 769P, BT-474, Caco-2, HCC 1954, MDA-MB-468, LnCAP, NRK-49F, SP2/0 cellelinier, musesplenocyter og kanin PBMC.
14. Fremgangsmåde ifølge krav 11, hvor den eukaryotiske cellevært er gærcelleværten.
15. Fremgangsmåde ifølge krav 14, hvor gærcelleværten er valgt blandt S. cerevisiae og en art af Pichia.
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| US201461924487P | 2014-01-07 | 2014-01-07 | |
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| US12347083B2 (en) * | 2021-06-25 | 2025-07-01 | Fei Company | Area selection in charged particle microscope imaging |
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| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
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| EP3447493A1 (en) | 2019-02-27 |
| CN113504375B (zh) | 2024-01-09 |
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| CN105849564A (zh) | 2016-08-10 |
| EP3092491A1 (en) | 2016-11-16 |
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| US11293929B2 (en) | 2022-04-05 |
| US20220341941A1 (en) | 2022-10-27 |
| EP3092491B1 (en) | 2018-10-31 |
| AU2020202783B2 (en) | 2022-08-25 |
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