DK3099800T3 - Ekspression og oprensning af crm197 og beslægtede proteiner - Google Patents

Ekspression og oprensning af crm197 og beslægtede proteiner Download PDF

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DK3099800T3
DK3099800T3 DK15743243.6T DK15743243T DK3099800T3 DK 3099800 T3 DK3099800 T3 DK 3099800T3 DK 15743243 T DK15743243 T DK 15743243T DK 3099800 T3 DK3099800 T3 DK 3099800T3
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crm197
purification
expression
related proteins
proteins
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DK15743243.6T
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Natalia Oganesyan
Andrew Lees
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Fina Biosolutions Llc
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K39/02Bacterial antigens
    • A61K39/05Actinobacteria, e.g. Actinomyces, Streptomyces, Nocardia, Bifidobacterium, Gardnerella, Corynebacterium; Propionibacterium
    • AHUMAN NECESSITIES
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    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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    • AHUMAN NECESSITIES
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    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • GPHYSICS
    • G01MEASURING; TESTING
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    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
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    • G01MEASURING; TESTING
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    • C07K2319/00Fusion polypeptide
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/34Assays involving biological materials from specific organisms or of a specific nature from bacteria from Corynebacterium (G)

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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
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DK15743243.6T 2014-01-31 2015-02-02 Ekspression og oprensning af crm197 og beslægtede proteiner DK3099800T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461934377P 2014-01-31 2014-01-31
PCT/US2015/014130 WO2015117093A1 (en) 2014-01-31 2015-02-02 Expression and purification of crm197 and related proteins

Publications (1)

Publication Number Publication Date
DK3099800T3 true DK3099800T3 (da) 2021-01-11

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DK15743243.6T DK3099800T3 (da) 2014-01-31 2015-02-02 Ekspression og oprensning af crm197 og beslægtede proteiner

Country Status (10)

Country Link
US (1) US10093704B2 (da)
EP (1) EP3099800B1 (da)
JP (2) JP2017510290A (da)
KR (1) KR102282930B1 (da)
CN (1) CN106661587B (da)
AU (1) AU2015210674B2 (da)
CA (1) CA2938251C (da)
DK (1) DK3099800T3 (da)
NZ (1) NZ722504A (da)
WO (1) WO2015117093A1 (da)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
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US10597664B2 (en) * 2014-01-31 2020-03-24 Fina Biosolutions, Llc Expression and purification of CRM proteins and related proteins, and protein domains
DK3099800T3 (da) * 2014-01-31 2021-01-11 Fina Biosolutions Llc Ekspression og oprensning af crm197 og beslægtede proteiner
CN106350527B (zh) * 2016-11-03 2019-05-07 中国人民解放军军事医学科学院生物工程研究所 一种可在大肠杆菌可溶性高表达的白喉毒素突变体
CN110366428B (zh) 2016-12-30 2024-05-17 Vaxcyte公司 具有非天然氨基酸的多肽-抗原缀合物
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
KR101908590B1 (ko) 2017-02-01 2018-10-16 (주)포바이오코리아 Crm197의 용해성 단백질 발현 및 정제 방법
EP3444269A1 (en) 2017-08-17 2019-02-20 National Research Council of Canada Systems and methods for the production of diphtheria toxin polypeptides
CN107365782A (zh) * 2017-08-29 2017-11-21 廊坊梅花生物技术开发有限公司 一种基因工程菌及其应用
WO2020010016A1 (en) 2018-07-04 2020-01-09 Sutrovax, Inc. Self-adjuvanted immunogenic conjugates
DK3817775T3 (da) 2018-07-04 2024-09-16 Vaxcyte Inc Forbedringer i immunogene konjugater
WO2020010000A1 (en) 2018-07-04 2020-01-09 Sutrovax, Inc. Improved methods for the preparation of immunogenic conjugates
ES2944837T3 (es) 2018-11-08 2023-06-26 Sutro Biopharma Inc Cepas de E. coli con citoplasma oxidativo
CN110452838B (zh) * 2019-07-18 2020-12-29 艾美卫信生物药业(浙江)有限公司 一种crm197菌种培养基、配制方法及发酵培养方法
KR102949308B1 (ko) * 2020-03-16 2026-04-06 피나 바이오솔루션스, 엘엘씨 용해성 재조합 단백질의 생산
US12351850B2 (en) 2020-04-30 2025-07-08 Sutro Biopharma, Inc. Methods of producing full-length antibodies using E. coli
CN118027189B (zh) * 2024-04-11 2024-12-06 上海惠盾因泰生物科技有限公司 一种抗白喉毒素突变体抗体及crm197的纯化制备方法和应用
WO2026034998A1 (ko) * 2024-08-07 2026-02-12 한국생명공학연구원 대장균 내 이황화 단백질의 폴딩 강화 방법

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DK3099800T3 (da) * 2014-01-31 2021-01-11 Fina Biosolutions Llc Ekspression og oprensning af crm197 og beslægtede proteiner

Also Published As

Publication number Publication date
NZ722504A (en) 2017-08-25
EP3099800B1 (en) 2020-10-14
JP7073312B2 (ja) 2022-05-23
US20160333057A1 (en) 2016-11-17
KR20160113276A (ko) 2016-09-28
JP2019205446A (ja) 2019-12-05
KR102282930B1 (ko) 2021-07-27
EP3099800A1 (en) 2016-12-07
WO2015117093A1 (en) 2015-08-06
US10093704B2 (en) 2018-10-09
AU2015210674B2 (en) 2017-08-24
AU2015210674A1 (en) 2016-08-18
CN106661587A (zh) 2017-05-10
CA2938251A1 (en) 2015-08-06
EP3099800A4 (en) 2017-11-08
CA2938251C (en) 2021-01-12
CN106661587B (zh) 2020-01-24
JP2017510290A (ja) 2017-04-13

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