DK3368655T3 - Forbedret proteinekspression og fremgangsmåder dertil - Google Patents
Forbedret proteinekspression og fremgangsmåder dertil Download PDFInfo
- Publication number
- DK3368655T3 DK3368655T3 DK16797679.4T DK16797679T DK3368655T3 DK 3368655 T3 DK3368655 T3 DK 3368655T3 DK 16797679 T DK16797679 T DK 16797679T DK 3368655 T3 DK3368655 T3 DK 3368655T3
- Authority
- DK
- Denmark
- Prior art keywords
- methods
- protein expression
- improved protein
- improved
- expression
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/50—Methods for regulating/modulating their activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/50—Biochemical production, i.e. in a transformed host cell
- C12N2330/51—Specially adapted vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/101—Plasmid DNA for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562248757P | 2015-10-30 | 2015-10-30 | |
| PCT/US2016/059078 WO2017075195A1 (en) | 2015-10-30 | 2016-10-27 | Enhanced protein expression and methods thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3368655T3 true DK3368655T3 (da) | 2020-09-14 |
Family
ID=57326477
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16797679.4T DK3368655T3 (da) | 2015-10-30 | 2016-10-27 | Forbedret proteinekspression og fremgangsmåder dertil |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US11306319B2 (da) |
| EP (1) | EP3368655B1 (da) |
| JP (1) | JP7025325B2 (da) |
| KR (1) | KR20180069057A (da) |
| CN (1) | CN108473946B (da) |
| DK (1) | DK3368655T3 (da) |
| WO (1) | WO2017075195A1 (da) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3519573A1 (en) * | 2016-09-30 | 2019-08-07 | Invista North America S.a.r.l. | Enhanced processes and reagents for host engineering |
| US11879127B2 (en) | 2017-08-23 | 2024-01-23 | Danisco Us Inc. | Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains |
| WO2019118463A1 (en) | 2017-12-15 | 2019-06-20 | Danisco Us Inc | Cas9 variants and methods of use |
| CN111670244B (zh) | 2018-01-03 | 2023-08-01 | 丹尼斯科美国公司 | 突变的和遗传修饰的芽孢杆菌属细胞及其用于增加蛋白质产生的方法 |
| US11407983B2 (en) | 2018-08-07 | 2022-08-09 | Cj Cheiljedang Corporation | Nucleic acid molecules comprising a variant RpoC coding sequence |
| JP2022526982A (ja) | 2019-04-05 | 2022-05-27 | ダニスコ・ユーエス・インク | 線状組換えＤＮＡコンストラクトを使用してバチルス（Ｂａｃｉｌｌｕｓ）のゲノムにドナーＤＮＡ配列を組み込むための方法及びその組成物 |
| CA3136113A1 (en) | 2019-04-05 | 2020-10-08 | Danisco Us Inc. | Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof |
| CN110129303B (zh) * | 2019-05-06 | 2022-04-01 | 武汉轻工大学 | 一种耐高温酸性的果胶酶TsPec及基因与应用 |
| EP3943603A1 (en) * | 2020-07-22 | 2022-01-26 | Technische Universität Darmstadt | Rapid transformation method for gram-positive bacteria |
| US20240294894A1 (en) * | 2021-06-24 | 2024-09-05 | Basf Se | Improved bacillus host cell with altered rema/remb protein |
| CN113862183A (zh) * | 2021-09-26 | 2021-12-31 | 上海农乐生物制品股份有限公司 | 一种多肽微生物菌剂及其制备方法和在制备多肽微生物肥料中的用途 |
| CN114214354A (zh) * | 2021-12-31 | 2022-03-22 | 天津科技大学 | 一种双载体表达体系及其构建方法与应用 |
| EP4627076A1 (en) | 2022-12-01 | 2025-10-08 | Danisco Us Inc | Iterative multiplex genome engineering in microbial cells using a recombinant self-excisable selection marker system |
| WO2024118881A1 (en) | 2022-12-01 | 2024-06-06 | Genencor International Bv | Iterative muliplex genome engineering in microbial cells using a bidirectional selection marker system |
| WO2024118882A1 (en) | 2022-12-01 | 2024-06-06 | Genencor International Bv | Iterative multiplex genome engineering in microbial cells using a selection marker swapping system |
| EP4630563A1 (en) * | 2022-12-05 | 2025-10-15 | Novozymes A/S | Modified rna polymerase activities |
| IT202300003192A1 (it) | 2023-02-23 | 2024-08-23 | Space S R L Con Unico Socio | Apparecchiatura di calibrazione e metodo di verifica del posizionamento di un dispositivo di calibrazione, in particolare per la calibrazione di uno o piu’ sensori adas |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5264366A (en) | 1984-05-29 | 1993-11-23 | Genencor, Inc. | Protease deficient bacillus |
| US5972682A (en) | 1984-05-29 | 1999-10-26 | Genencor International, Inc. | Enzymatically active modified subtilisins |
| GB9614700D0 (en) | 1996-07-12 | 1996-09-04 | Medical Res Council | Over-expression of proteins in E.Coli |
| WO1998022598A1 (en) * | 1996-11-18 | 1998-05-28 | Novo Nordisk Biotech, Inc. | Methods for producing polypeptides in surfactin mutants of bacillus cells |
| JP4085523B2 (ja) | 1999-06-25 | 2008-05-14 | 味の素株式会社 | 自然形質転換能を有する納豆菌 |
| US6509185B1 (en) | 2000-01-07 | 2003-01-21 | Genencor International, Inc. | Mutant aprE promotor |
| ES2275843T3 (es) * | 2001-03-30 | 2007-06-16 | The National Food Research Institute | Metodo para aumentar la produccion de un antibiotico en una bacteria mediante la introduccion de mutaciones que confieren una resistencia a los antibioticos. |
| US7314712B2 (en) | 2001-07-27 | 2008-01-01 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Systems for in vivo site-directed mutagenesis using oligonucleotides |
| US20050095669A1 (en) | 2001-12-29 | 2005-05-05 | Joergensen Steen T. | Eubacterial rna-polymerase mutants with altered product production |
| BRPI0509671A (pt) | 2004-04-08 | 2007-10-30 | Genencor Internation Inc | alfa-amilases mutante |
| WO2008112459A2 (en) | 2007-03-09 | 2008-09-18 | Danisco Us Inc., Genencor Division | Alkaliphilic bacillus species a-amylase variants, compositions comprising a-amylase variants, and methods of use |
| WO2009149419A2 (en) | 2008-06-06 | 2009-12-10 | Danisco Us Inc. | Variant alpha-amylases from bacillus subtilis and methods of use, thereof |
| US20100022861A1 (en) | 2008-07-28 | 2010-01-28 | Medtronic, Inc. | Implantable optical hemodynamic sensor including an extension member |
| WO2010056635A1 (en) | 2008-11-11 | 2010-05-20 | Danisco Us Inc. | Compositions and methods comprising a subtilisin variant |
| CN102405283B (zh) | 2009-04-17 | 2016-09-14 | 丹尼斯科美国公司 | 用于不进行pH调整的谷物加工的组合物和方法 |
| AR076941A1 (es) | 2009-06-11 | 2011-07-20 | Danisco Us Inc | Cepa de bacillus para una mayor produccion de proteina |
| CN102492639B (zh) * | 2011-11-29 | 2013-03-06 | 南京大学 | 利用rna聚合酶突变提高环脂肽类抗生素产量的突变体及制法和应用 |
| US20140329309A1 (en) | 2011-12-09 | 2014-11-06 | Danisco Us Inc. | Ribosomal Promoters for Production in Microorganisms |
| EP2970931B1 (en) | 2013-03-11 | 2017-12-06 | Danisco US Inc. | Alpha-amylase combinatorial variants |
| KR101594156B1 (ko) * | 2013-06-25 | 2016-02-15 | 씨제이제일제당 주식회사 | Ｌ-라이신 생산능이 향상된 미생물 및 그를 이용하여 ｌ-라이신을 생산하는 방법 |
| CN104974946B (zh) * | 2014-04-08 | 2019-01-11 | 中国科学院天津工业生物技术研究所 | 耐高渗透压的重组大肠杆菌及其应用 |
-
2016
- 2016-10-27 KR KR1020187014743A patent/KR20180069057A/ko not_active Ceased
- 2016-10-27 WO PCT/US2016/059078 patent/WO2017075195A1/en not_active Ceased
- 2016-10-27 DK DK16797679.4T patent/DK3368655T3/da active
- 2016-10-27 US US15/771,618 patent/US11306319B2/en active Active
- 2016-10-27 JP JP2018522644A patent/JP7025325B2/ja active Active
- 2016-10-27 EP EP16797679.4A patent/EP3368655B1/en active Active
- 2016-10-27 CN CN201680077278.6A patent/CN108473946B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP7025325B2 (ja) | 2022-02-24 |
| CN108473946A (zh) | 2018-08-31 |
| US20180327757A1 (en) | 2018-11-15 |
| EP3368655B1 (en) | 2020-06-10 |
| WO2017075195A1 (en) | 2017-05-04 |
| KR20180069057A (ko) | 2018-06-22 |
| US11306319B2 (en) | 2022-04-19 |
| CN108473946B (zh) | 2023-01-03 |
| EP3368655A1 (en) | 2018-09-05 |
| JP2018532413A (ja) | 2018-11-08 |
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