DK3119882T3 - Fremgangsmåder til produktion af rekombinante glycoproteiner med modificeret glycosylering - Google Patents

Fremgangsmåder til produktion af rekombinante glycoproteiner med modificeret glycosylering Download PDF

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Publication number
DK3119882T3
DK3119882T3 DK15770138.4T DK15770138T DK3119882T3 DK 3119882 T3 DK3119882 T3 DK 3119882T3 DK 15770138 T DK15770138 T DK 15770138T DK 3119882 T3 DK3119882 T3 DK 3119882T3
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DK
Denmark
Prior art keywords
procedures
production
modified glycosylation
recombinant glycoproteins
glycoproteins
Prior art date
Application number
DK15770138.4T
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English (en)
Inventor
Nien-Yi Chen
Che-Haorz Wu
Hung-Chi Chen
Winston Town
Original Assignee
Oneness Biotech Co Ltd
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Publication date
Application filed by Oneness Biotech Co Ltd filed Critical Oneness Biotech Co Ltd
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Publication of DK3119882T3 publication Critical patent/DK3119882T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/42Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4283Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig
    • C07K16/4291Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig against IgE
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01051Alpha-L-fucosidase (3.2.1.51)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01096Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/40Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41Glycosylation, sialylation, or fucosylation
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DK15770138.4T 2014-03-17 2015-03-17 Fremgangsmåder til produktion af rekombinante glycoproteiner med modificeret glycosylering DK3119882T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461954337P 2014-03-17 2014-03-17
PCT/IB2015/001203 WO2015145268A2 (en) 2014-03-17 2015-03-17 Methods for producing recombinant glycoproteins with modified glycosylation

Publications (1)

Publication Number Publication Date
DK3119882T3 true DK3119882T3 (da) 2022-01-31

Family

ID=54068262

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15770138.4T DK3119882T3 (da) 2014-03-17 2015-03-17 Fremgangsmåder til produktion af rekombinante glycoproteiner med modificeret glycosylering

Country Status (19)

Country Link
US (2) US9540673B2 (da)
EP (1) EP3119882B1 (da)
JP (2) JP6701089B2 (da)
KR (1) KR102333923B1 (da)
CN (1) CN106715686B (da)
AU (1) AU2015237951B2 (da)
BR (1) BR112016021284A2 (da)
CA (1) CA2942618A1 (da)
DK (1) DK3119882T3 (da)
ES (1) ES2904993T3 (da)
HU (1) HUE057904T2 (da)
IL (1) IL247776B (da)
MX (1) MX382334B (da)
PL (1) PL3119882T3 (da)
PT (1) PT3119882T (da)
RU (1) RU2704830C2 (da)
SG (1) SG11201607759RA (da)
TW (1) TWI682033B (da)
WO (1) WO2015145268A2 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3149045T3 (da) * 2014-05-27 2023-03-06 Academia Sinica Sammensætninger og fremgangsmåder vedrørende universelle glycoformer til forbedret antistofeffekt
EP4010470A4 (en) 2019-08-05 2023-08-09 Cho Pharma Inc FUSION PROTEIN FOR REMODELING ANTIBODY GLYCOFORMS
CN110885809B (zh) * 2019-12-04 2021-08-17 中国农业大学 α-L-岩藻糖苷酶及其相关生物材料与应用
AR126089A1 (es) 2021-06-07 2023-09-13 Amgen Inc Uso de fucosidasa para controlar el nivel de afucosilación de proteínas glucosiladas
CN116621988A (zh) * 2022-02-10 2023-08-22 中国科学院上海药物研究所 一类亲和片段共价修饰的糖苷内切酶,其制备方法及在糖蛋白的糖基化改造中的应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3657899A (en) 1998-04-20 1999-11-08 James E. Bailey Glycosylation engineering of antibodies for improving antibody-dependent cellular cytotoxicity
CN101466402A (zh) * 2006-06-09 2009-06-24 马里兰卅大学(巴尔的摩) 糖基化改造的抗体治疗
EP2035034A4 (en) * 2006-06-09 2009-11-18 Univ Maryland GLYCOSYLATION-CONTROLLED ANTIBODY THERAPY
GB0624874D0 (en) * 2006-12-13 2007-01-24 Hansa Medical Ab Treatment
CA2679732A1 (en) * 2007-03-07 2008-09-18 Glycofi, Inc. Production of glycoproteins with modified fucosylation
KR101054362B1 (ko) * 2008-07-03 2011-08-05 재단법인 목암생명공학연구소 재조합 단백질의 푸코스 함량을 감소시키는 방법
CN102216452B (zh) * 2008-09-26 2013-08-21 尤里卡治疗公司 具有变异糖基化方式的细胞系和蛋白质
GB0821100D0 (en) * 2008-11-18 2008-12-24 Hansa Medical Ab Antibodies
EP2401378B1 (en) * 2009-02-25 2013-08-14 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
CN102511007B (zh) * 2009-10-02 2015-04-15 罗切格利卡特公司 抗体中的a-岩藻糖基化检测
US10087236B2 (en) * 2009-12-02 2018-10-02 Academia Sinica Methods for modifying human antibodies by glycan engineering
EP2812442B9 (en) * 2012-02-10 2023-02-15 University of Maryland, Baltimore Chemoenzymatic glycoengineering of antibodies and fc fragments thereof

Also Published As

Publication number Publication date
CN106715686A (zh) 2017-05-24
AU2015237951B2 (en) 2021-07-01
PT3119882T (pt) 2022-01-20
WO2015145268A8 (en) 2015-12-03
RU2016140528A (ru) 2018-04-17
SG11201607759RA (en) 2016-10-28
US20170145463A1 (en) 2017-05-25
TWI682033B (zh) 2020-01-11
MX382334B (es) 2025-03-13
CA2942618A1 (en) 2015-10-01
US9540673B2 (en) 2017-01-10
US10745729B2 (en) 2020-08-18
TW201623620A (zh) 2016-07-01
HUE057904T2 (hu) 2022-06-28
JP6701089B2 (ja) 2020-05-27
EP3119882B1 (en) 2021-11-17
RU2016140528A3 (da) 2018-10-18
EP3119882A2 (en) 2017-01-25
KR102333923B1 (ko) 2021-12-02
KR20160127830A (ko) 2016-11-04
CN106715686B (zh) 2021-03-16
RU2704830C2 (ru) 2019-10-31
IL247776A0 (en) 2016-11-30
EP3119882A4 (en) 2017-08-16
BR112016021284A2 (pt) 2017-10-03
JP2020072667A (ja) 2020-05-14
JP2017507664A (ja) 2017-03-23
ES2904993T3 (es) 2022-04-06
US20150259720A1 (en) 2015-09-17
WO2015145268A3 (en) 2016-01-21
PL3119882T3 (pl) 2022-05-16
MX2016012085A (es) 2017-05-23
IL247776B (en) 2021-05-31
WO2015145268A2 (en) 2015-10-01
AU2015237951A1 (en) 2016-10-20

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