DK3177634T3 - Modificerede nukleotidlinkere - Google Patents

Modificerede nukleotidlinkere Download PDF

Info

Publication number
DK3177634T3
DK3177634T3 DK15750100.8T DK15750100T DK3177634T3 DK 3177634 T3 DK3177634 T3 DK 3177634T3 DK 15750100 T DK15750100 T DK 15750100T DK 3177634 T3 DK3177634 T3 DK 3177634T3
Authority
DK
Denmark
Prior art keywords
modified nucleotide
nucleotide linkers
linkers
modified
nucleotide
Prior art date
Application number
DK15750100.8T
Other languages
English (en)
Inventor
Xiaolin Wu
Xiaohai Liu
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Application granted granted Critical
Publication of DK3177634T3 publication Critical patent/DK3177634T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/14—Pyrrolo-pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK15750100.8T 2014-08-08 2015-08-06 Modificerede nukleotidlinkere DK3177634T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1414098.2A GB201414098D0 (en) 2014-08-08 2014-08-08 Modified nucleotide linkers
PCT/GB2015/052282 WO2016020691A1 (en) 2014-08-08 2015-08-06 Modified nucleotide linkers

Publications (1)

Publication Number Publication Date
DK3177634T3 true DK3177634T3 (da) 2020-03-02

Family

ID=51629525

Family Applications (2)

Application Number Title Priority Date Filing Date
DK19178987.4T DK3564252T3 (da) 2014-08-08 2015-08-06 Modificerede nukleotidlinkere
DK15750100.8T DK3177634T3 (da) 2014-08-08 2015-08-06 Modificerede nukleotidlinkere

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK19178987.4T DK3564252T3 (da) 2014-08-08 2015-08-06 Modificerede nukleotidlinkere

Country Status (19)

Country Link
US (3) US10190157B2 (da)
EP (2) EP3177634B1 (da)
JP (3) JP6563482B2 (da)
KR (2) KR102287718B1 (da)
CN (2) CN111704643A (da)
AU (2) AU2015298469B2 (da)
BR (2) BR122020018717B1 (da)
CA (1) CA2957382C (da)
DK (2) DK3564252T3 (da)
ES (2) ES2875557T3 (da)
GB (1) GB201414098D0 (da)
IL (1) IL250498B (da)
MX (2) MX385213B (da)
MY (1) MY195167A (da)
NZ (1) NZ728810A (da)
RU (1) RU2699993C2 (da)
SG (3) SG10202000850XA (da)
WO (1) WO2016020691A1 (da)
ZA (1) ZA201700838B (da)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201414098D0 (en) * 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
WO2016182984A1 (en) * 2015-05-08 2016-11-17 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
EP3356381A4 (en) 2015-09-28 2019-06-12 The Trustees of Columbia University in the City of New York DESIGN AND SYNTHESIS OF NUCLEOTIDES BASED ON NOVEL DISULFIDE COMPOUNDS AS REVERSIBLE TERMINATORS FOR DNA SEQUENCING BY SYNTHESIS
US11266673B2 (en) 2016-05-23 2022-03-08 The Trustees Of Columbia University In The City Of New York Nucleotide derivatives and methods of use thereof
MX2019006616A (es) 2016-12-22 2019-10-24 Illumina Inc Arreglo que incluye cebador secuenciador y entidad no secuenciadora.
US11591647B2 (en) 2017-03-06 2023-02-28 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (SBS) methods that combine SBS cycle steps
CN119639879A (zh) 2017-03-28 2025-03-18 哥伦比亚大学董事会 用于连接荧光标记与用于dna合成测序的碱基的具有不同可裂解连接子的3'-o-改性的核苷酸类似物
CN110650968B (zh) * 2017-10-11 2022-07-05 深圳华大智造科技股份有限公司 修饰的核苷或核苷酸
US12215124B2 (en) 2018-02-21 2025-02-04 Singular Genomics Systems, Inc. Fluorinated nucleotides and uses thereof
US12188086B2 (en) 2018-03-15 2025-01-07 The Trustees Of Columbia University In The City Of New York Nucleotide analogues and use thereof for nucleic acid sequencing and analysis preliminary class
US10738072B1 (en) 2018-10-25 2020-08-11 Singular Genomics Systems, Inc. Nucleotide analogues
JP7542444B2 (ja) * 2018-12-14 2024-08-30 イルミナ ケンブリッジ リミテッド 配列決定の間のアンラベル化ヌクレオチドによるフェージング減少
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
CN113453692A (zh) 2019-01-08 2021-09-28 奇异基因组学系统公司 核苷酸可裂解接头和其用途
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
AU2020230955B2 (en) 2019-03-01 2025-06-05 Illumina Cambridge Limited Exocyclic amine-substituted coumarin compounds and their uses as fluorescent labels
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
KR102865446B1 (ko) * 2019-03-01 2025-09-29 일루미나 케임브리지 리미티드 삼차 아민 치환된 쿠마린 화합물 및 형광 표지로서의 이의 용도
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
CN114829369A (zh) 2019-11-27 2022-07-29 伊鲁米纳剑桥有限公司 含环辛四烯的染料和组合物
JP7287299B2 (ja) 2020-01-31 2023-06-06 トヨタ自動車株式会社 車両および車両制御インターフェース
IL316161A (en) 2020-06-22 2024-12-01 Illumina Cambridge Ltd Nucleotides and nucleosides with a 3' masking group
US11981964B2 (en) 2020-07-28 2024-05-14 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195196A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Alkylpyridinium coumarin dyes and uses in sequencing applications
US20220195517A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Long stokes shift chromenoquinoline dyes and uses in sequencing applications
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
JP2024516191A (ja) 2021-05-05 2024-04-12 イルミナ ケンブリッジ リミテッド ビス－ホウ素縮合複素環を含有する蛍光色素及び配列決定における使用
BR112023024130A2 (pt) 2021-05-20 2024-01-30 Illumina Cambridge Ltd Composições e métodos para sequenciamento por síntese
JP2024520279A (ja) 2021-05-26 2024-05-24 イルミナ インコーポレイテッド ３’－ブロック化ヌクレオチドの精製及び重合
US20230304086A1 (en) 2022-03-28 2023-09-28 Illumina Cambridge Limited Labeled avidin and methods for sequencing
EP4499872B1 (en) 2022-03-29 2026-04-29 Illumina, Inc. Systems and methods of sequencing polynucleotides
AU2023244351A1 (en) 2022-03-31 2024-01-18 Illumina, Inc. Nucleosides and nucleotides with 3' vinyl blocking group useful in sequencing by synthesis
US20230357845A1 (en) 2022-03-31 2023-11-09 Illumina, Inc. Compositions and methods for improving sequencing signals
CA3251383A1 (en) 2022-05-31 2023-12-07 Illumina, Inc NUCLEIC ACID COMPOSITIONS AND SEQUENCE METHODS
WO2024003087A1 (en) 2022-06-28 2024-01-04 Illumina, Inc. Fluorescent dyes containing fused tetracyclic bis-boron heterocycle and uses in sequencing
CA3246518A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Compositions and methods for reducing photolesions during sequencing
CA3246545A1 (en) 2022-12-22 2024-06-27 Illumina, Inc. PALLADIUM CATALYST COMPOSITIONS AND SYNTHETIC SEQUENCE PROCESSES
EP4638795A1 (en) 2022-12-22 2025-10-29 Illumina, Inc. Transition-metal catalyst compositions and methods for sequencing by synthesis
CN119095984A (zh) 2022-12-27 2024-12-06 伊路米纳有限公司 使用3'烯丙基封端的核苷酸的测序方法
WO2024206407A2 (en) 2023-03-29 2024-10-03 Illumina, Inc. Naphthalimide dyes and uses in nucleic acid sequencing
CN119452101A (zh) 2023-03-30 2025-02-14 因美纳公司 用于核酸测序的组合物和方法
US20250075269A1 (en) 2023-08-31 2025-03-06 Illumina, Inc. Compositions and methods for nucleic acid sequencing
US20250137048A1 (en) 2023-10-26 2025-05-01 Illumina, Inc. 4,5-substituted naphthalimide dyes and uses in nucleic acid sequencing
WO2025106715A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Conjugated polymers or polymer dots as fluorescent labels
WO2025136890A1 (en) 2023-12-18 2025-06-26 Illumina, Inc. Hydrogel nanoparticles as labeling scaffold in sequencing
US20250215493A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
WO2025144711A1 (en) 2023-12-29 2025-07-03 Illumina, Inc. Tricyclic polymethine dyes for nucleic acid sequencing
WO2025184226A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. Nucleotides with terminal phosphate capping
WO2025188906A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Modified adenosine nucleotides
WO2025221895A1 (en) 2024-04-19 2025-10-23 Illumina, Inc. Water soluble polymer scaffolds for dye labeling
WO2026035914A1 (en) 2024-08-09 2026-02-12 Illumina, Inc. Nucleotides having 3'-hydroxy blocking groups

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
DE3924454A1 (de) 1989-07-24 1991-02-07 Cornelis P Prof Dr Hollenberg Die anwendung von dna und dna-technologie fuer die konstruktion von netzwerken zur verwendung in der chip-konstruktion und chip-produktion (dna chips)
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
JP2001524926A (ja) 1991-09-18 2001-12-04 アフィマックス テクノロジーズ ナームロゼ フェンノートシャップ オリゴマーの雑多ライブラリーの集合体を合成する方法
DE69233501T2 (de) 1991-11-22 2006-02-23 Affymetrix, Inc. (n.d.Ges.d.Staates Delaware), Santa Clara Kombinatorische Strategien für die Polymersynthese
WO1993017126A1 (en) 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5583211A (en) 1992-10-29 1996-12-10 Beckman Instruments, Inc. Surface activated organic polymers useful for location - specific attachment of nucleic acids, peptides, proteins and oligosaccharides
US5472672A (en) 1993-10-22 1995-12-05 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for polymer synthesis using arrays
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
JPH09507121A (ja) 1993-10-26 1997-07-22 アフィマックス テクノロジーズ ナームロゼ ベノートスハップ 生物学的チップ上の核酸プローブアレー
US5429807A (en) 1993-10-28 1995-07-04 Beckman Instruments, Inc. Method and apparatus for creating biopolymer arrays on a solid support surface
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5545531A (en) 1995-06-07 1996-08-13 Affymax Technologies N.V. Methods for making a device for concurrently processing multiple biological chip assays
DE69638321D1 (de) 1995-10-11 2011-03-03 Luminex Corp Gleichzeitige mehrfachanalyse klinischer proben
US5658734A (en) 1995-10-17 1997-08-19 International Business Machines Corporation Process for synthesizing chemical compounds
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6297006B1 (en) 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
US6087102A (en) 1998-01-07 2000-07-11 Clontech Laboratories, Inc. Polymeric arrays and methods for their use in binding assays
US6287776B1 (en) 1998-02-02 2001-09-11 Signature Bioscience, Inc. Method for detecting and classifying nucleic acid hybridization
JP3944996B2 (ja) 1998-03-05 2007-07-18 株式会社日立製作所 Ｄｎａプローブアレー
US6031078A (en) 1998-06-16 2000-02-29 Millennium Pharmaceuticals, Inc. MTbx protein and nucleic acid molecules and uses therefor
US6277628B1 (en) 1998-10-02 2001-08-21 Incyte Genomics, Inc. Linear microarrays
WO2000036151A1 (en) * 1998-12-14 2000-06-22 Li-Cor, Inc. A heterogeneous assay for pyrophosphate detection
US6379698B1 (en) * 1999-04-06 2002-04-30 Isis Pharmaceuticals, Inc. Fusogenic lipids and vesicles
EP1923471B1 (en) 1999-04-20 2012-12-19 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20050181440A1 (en) 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
JP2004516810A (ja) 2000-06-07 2004-06-10 リ−コール インコーポレーティッド 電荷スイッチヌクレオチド
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AU2002258997A1 (en) * 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
DE10120798B4 (de) * 2001-04-27 2005-12-29 Genovoxx Gmbh Verfahren zur Bestimmung der Genexpression
DE10120797B4 (de) 2001-04-27 2005-12-22 Genovoxx Gmbh Verfahren zur Analyse von Nukleinsäureketten
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7414116B2 (en) * 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
ES2864086T3 (es) 2002-08-23 2021-10-13 Illumina Cambridge Ltd Nucleótidos etiquetados
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
EP1616034A4 (en) * 2003-04-01 2008-12-03 Activx Biosciences Inc ACYL PHOSPHATE AND PHOSPHONATE EASTS AND PROCESSES FOR THEIR SYNTHESIS AND USE IN PROTEAM ANALYSIS
EP1725572B1 (de) * 2003-11-05 2017-05-31 AGCT GmbH Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
US20070117104A1 (en) 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US8212020B2 (en) 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
EP1885876A2 (de) * 2005-03-17 2008-02-13 Genovoxx GmbH Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US8178360B2 (en) 2006-05-18 2012-05-15 Illumina Cambridge Limited Dye compounds and the use of their labelled conjugates
US8110559B2 (en) 2006-09-15 2012-02-07 Enzon Pharmaceuticals, Inc. Hindered ester-based biodegradable linkers for oligonucleotide delivery
MX2009002855A (es) * 2006-09-15 2009-03-30 Enzon Pharmaceuticals Inc Profarmacos polimericos dirigidos que contienen enlazadores multifuncionales.
EP2063912A4 (en) 2006-09-15 2010-07-28 Enzon Pharmaceuticals Inc POLYALKYLENE OXIDES HAVING BIODEGRADABLE BOND SEGMENTS BASED ON COARSE ESTER
JP2010503707A (ja) * 2006-09-15 2010-02-04 エンゾン ファーマスーティカルズ インコーポレイテッド オリゴヌクレオチドの送達を目的としたヒンダードエステル系生体分解性リンカー
JP2010503706A (ja) * 2006-09-15 2010-02-04 エンゾン ファーマスーティカルズ インコーポレイテッド リジン系ポリマーリンカー
US8399188B2 (en) * 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
MX2009005936A (es) * 2006-12-05 2010-01-15 Lasergen Inc Nucleotidos y nucleosidos marcados de foto-escision y nucleotidos y nucleosidos marcados y metodos para su uso en secunciacion de adn.
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7948015B2 (en) 2006-12-14 2011-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US9163053B2 (en) * 2007-05-18 2015-10-20 Fluidigm Corporation Nucleotide analogs
US7678894B2 (en) * 2007-05-18 2010-03-16 Helicos Biosciences Corporation Nucleotide analogs
DK2725107T3 (da) 2007-10-19 2019-01-02 Univ Columbia DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer
WO2009051807A1 (en) * 2007-10-19 2009-04-23 The Trustees Of Columbia University In The City Of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9206216B2 (en) * 2010-04-21 2015-12-08 Pierce Biotechnology, Inc. Modified nucleotides methods and kits
WO2012159072A2 (en) * 2011-05-18 2012-11-22 Cayman Chemical Company, Incorporated Fluorescent molecular probes for use in assays that measure test compound competitive binding with sam-utilizing proteins
CA2859660C (en) 2011-09-23 2021-02-09 Illumina, Inc. Methods and compositions for nucleic acid sequencing
RU2014117678A (ru) * 2011-11-17 2015-12-27 Индиана Юниверсити Рисерч Энд Текнолоджи Корпорейшн Пептиды глюкагонового суперсемейства, обладающие глюкокортикоидной рецепторной активностью
EP2797634A4 (en) * 2011-12-29 2015-08-05 Moderna Therapeutics Inc MODIFIED MRNAS FOR THE CODING OF CELL-PENETRATING POLYPEPTIDES
CN103087131B (zh) * 2013-01-15 2015-07-08 上海交通大学 可逆终端及其合成和在dna合成测序中的用途
CA2902992C (en) 2013-03-08 2020-08-25 Illumina Cambridge Ltd Rhodamine compounds and their use as fluorescent labels
AU2013380645B2 (en) 2013-03-08 2018-03-15 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
CN103484106B (zh) 2013-09-05 2015-08-19 上海交通大学 四色荧光标记可逆终端及其在dna测序中的用途
CN103866010B (zh) 2014-02-28 2016-04-20 郭诚 一种基因测序的方法
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers

Also Published As

Publication number Publication date
KR102287718B1 (ko) 2021-08-09
EP3177634A1 (en) 2017-06-14
JP2022000435A (ja) 2022-01-04
RU2017103539A (ru) 2018-09-10
IL250498B (en) 2021-03-25
AU2018278843A1 (en) 2019-01-03
CA2957382A1 (en) 2016-02-11
US11230734B2 (en) 2022-01-25
RU2699993C2 (ru) 2019-09-12
IL250498A0 (en) 2017-03-30
JP6563482B2 (ja) 2019-08-21
ZA201700838B (en) 2019-06-26
ES2875557T3 (es) 2021-11-10
US20220186306A1 (en) 2022-06-16
BR112017002251A8 (pt) 2020-05-12
BR112017002251B1 (pt) 2022-01-25
BR112017002251A2 (pt) 2019-04-09
AU2018278843B2 (en) 2019-07-18
WO2016020691A1 (en) 2016-02-11
KR20170039743A (ko) 2017-04-11
ES2773438T3 (es) 2020-07-13
NZ748150A (en) 2023-03-31
KR20210100205A (ko) 2021-08-13
CA2957382C (en) 2022-05-31
BR122020018717B1 (pt) 2022-01-25
MX2017001694A (es) 2017-04-27
EP3564252A1 (en) 2019-11-06
AU2015298469A1 (en) 2017-02-23
AU2015298469B2 (en) 2018-12-06
US20190153529A1 (en) 2019-05-23
CN106604926A (zh) 2017-04-26
MX377448B (es) 2025-03-10
EP3564252B1 (en) 2021-03-17
DK3564252T3 (da) 2021-05-25
SG10202000850XA (en) 2020-03-30
MX2020009401A (es) 2021-08-09
US10190157B2 (en) 2019-01-29
JP2020015722A (ja) 2020-01-30
SG11201700880RA (en) 2017-03-30
NZ728810A (en) 2018-11-30
KR102491869B1 (ko) 2023-01-26
JP2017525695A (ja) 2017-09-07
MX385213B (es) 2025-03-14
US20160040225A1 (en) 2016-02-11
EP3177634B1 (en) 2019-12-18
CN106604926B (zh) 2020-05-15
GB201414098D0 (en) 2014-09-24
SG10201900948PA (en) 2019-03-28
JP7726707B2 (ja) 2025-08-20
MY195167A (en) 2023-01-11
US11773438B2 (en) 2023-10-03
CN111704643A (zh) 2020-09-25
RU2017103539A3 (da) 2018-11-15

Similar Documents

Publication Publication Date Title
DK3564252T3 (da) Modificerede nukleotidlinkere
DK3183051T3 (da) Væske-til-luftmembranenergivekslere
DK3134402T3 (da) 4-amino-imidazoquinolinforbindelser
DK3188836T3 (da) Dna-syntese
DK3094728T3 (da) Kiralt design
DK3006072T3 (da) Karadgang
DK3211743T3 (da) Ø-til-netværk-omskiftningsfremgangsmåde
DK3222714T3 (da) Phytasemutanter
DK3174483T3 (da) Hyporørkonstruktion
DK3119396T3 (da) Muscarinreceptoragonister
EP3230438C0 (en) N-GLYCOSYLATION
DK3110442T3 (da) Modificerede meningokok-fhbp-polypeptider
DK4242916T3 (da) Forstøver
DK3183340T3 (da) Termolabile exonukleaser
EP4011484C0 (en) CARBONIC ANHYDRASE OF THERMOVIBRIO AMMONIFICANS
DE112015001664A5 (de) Betätigungsaktuator
EP4546794C0 (en) AUDIO CONNECTION CONCEPT
DK3154703T3 (da) Fuldkappe-snekkecentrifuge
DE112015005041A5 (de) Gargerätevorrichtung
DK3110439T3 (da) Pcsk9-vacciner
DE102014000623A8 (de) Halbhohlnietelement
DE202014005655U8 (de) llluminationsvorrichtung
DE112015001633A5 (de) Betätigungsaktuator
DK3447493T3 (da) Proteinrettede ortologer
ES1133830Y (es) Columbario