DK3186376T3 - Fremgangsmåder til forbedring af cas9-medieret manipulationseffektivitet - Google Patents

Fremgangsmåder til forbedring af cas9-medieret manipulationseffektivitet Download PDF

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DK3186376T3
DK3186376T3 DK15763698.6T DK15763698T DK3186376T3 DK 3186376 T3 DK3186376 T3 DK 3186376T3 DK 15763698 T DK15763698 T DK 15763698T DK 3186376 T3 DK3186376 T3 DK 3186376T3
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methods
manipulation efficiency
cas9
improving
mediated manipulation
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DK15763698.6T
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Peter Sean Cameron
Rachel E Haurwitz
Andrew P May
Christopher H Nye
Overbeek Megan Van
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Caribou Biosciences Inc
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/30—Endoribonucleases active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters (3.1.30)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK15763698.6T 2014-08-27 2015-08-26 Fremgangsmåder til forbedring af cas9-medieret manipulationseffektivitet DK3186376T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462042358P 2014-08-27 2014-08-27
US201462047495P 2014-09-08 2014-09-08
PCT/US2015/047046 WO2016033246A1 (en) 2014-08-27 2015-08-26 Methods for increasing cas9-mediated engineering efficiency

Publications (1)

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DK3186376T3 true DK3186376T3 (da) 2019-05-06

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US (2) US9970030B2 (da)
EP (1) EP3186376B1 (da)
AU (1) AU2015308910B2 (da)
CA (1) CA2959070C (da)
DK (1) DK3186376T3 (da)
ES (1) ES2730378T3 (da)
GB (1) GB2544001A (da)
IL (1) IL250700B (da)
NZ (1) NZ728437A (da)
SG (1) SG11201701245QA (da)
WO (1) WO2016033246A1 (da)

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US20180171360A1 (en) 2018-06-21
SG11201701245QA (en) 2017-03-30
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ES2730378T3 (es) 2019-11-11
IL250700A0 (en) 2017-04-30
EP3186376B1 (en) 2019-03-20
CA2959070C (en) 2020-11-10
US20160257973A1 (en) 2016-09-08
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