DK3218509T3 - Mutante mikroorganismer, der er resistente over for lactose-drab - Google Patents
Mutante mikroorganismer, der er resistente over for lactose-drab Download PDFInfo
- Publication number
- DK3218509T3 DK3218509T3 DK15797912.1T DK15797912T DK3218509T3 DK 3218509 T3 DK3218509 T3 DK 3218509T3 DK 15797912 T DK15797912 T DK 15797912T DK 3218509 T3 DK3218509 T3 DK 3218509T3
- Authority
- DK
- Denmark
- Prior art keywords
- mutant microorganisms
- microorganisms resistant
- lactose killing
- killing
- lactose
- Prior art date
Links
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 title 1
- 239000008101 lactose Substances 0.000 title 1
- 244000005700 microbiome Species 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01022—Lactose synthase (2.4.1.22)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14193151 | 2014-11-14 | ||
| PCT/EP2015/076449 WO2016075243A1 (en) | 2014-11-14 | 2015-11-12 | Mutant microorganisms resistant to lactose killing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3218509T3 true DK3218509T3 (da) | 2024-05-21 |
Family
ID=51893928
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15797912.1T DK3218509T3 (da) | 2014-11-14 | 2015-11-12 | Mutante mikroorganismer, der er resistente over for lactose-drab |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US10858684B2 (da) |
| EP (1) | EP3218509B1 (da) |
| JP (2) | JP6765372B2 (da) |
| KR (1) | KR102579346B1 (da) |
| CN (2) | CN107075506B (da) |
| AU (2) | AU2015345073B9 (da) |
| BR (1) | BR112017006295B1 (da) |
| CA (1) | CA2958786A1 (da) |
| DK (1) | DK3218509T3 (da) |
| ES (1) | ES2985061T3 (da) |
| RU (2) | RU2021109889A (da) |
| SG (1) | SG11201701585VA (da) |
| WO (1) | WO2016075243A1 (da) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017031189A1 (en) * | 2015-08-17 | 2017-02-23 | Zuchem, Inc. | Production of activated tdp-deoxysugars in recombinant microorganisms |
| KR101731263B1 (ko) * | 2016-04-25 | 2017-05-02 | 서울대학교 산학협력단 | 코리네박테리움 글루타미쿰을 이용한 2'-푸코실락토오스의 생산방법 |
| ES2856749T3 (es) * | 2016-10-29 | 2021-09-28 | Chr Hansen Hmo Gmbh | Proceso para la producción de oligosacáridos fucosilados |
| US11535878B2 (en) | 2016-12-27 | 2022-12-27 | Inbiose N.V. | In vivo synthesis of sialylated compounds |
| AU2018374516B2 (en) * | 2017-12-01 | 2024-03-07 | Societe Des Produits Nestle S.A. | Human milk fortifier composition |
| WO2019209241A1 (en) * | 2018-04-23 | 2019-10-31 | Dupont Nutrition Biosciences Aps | Increasing export of 2' fucosyllactose from microbial cells through the expression of a heterologous nucleic acid |
| WO2019209245A1 (en) * | 2018-04-23 | 2019-10-31 | Dupont Nutrition Biosciences Aps | Increasing activity of 2' fucosyllactose transporters endogenous to microbial cells |
| US10519475B1 (en) * | 2018-11-21 | 2019-12-31 | Amyris, Inc. | Biosynthesis of compounds in yeast |
| CA3121848A1 (en) | 2018-12-18 | 2020-06-25 | Inbiose N.V. | Production of 3-fucosyllactose and lactose converting .alpha.-1,3-fucosyltransferase enzymes |
| CN109735479B (zh) * | 2019-01-30 | 2022-04-01 | 光明乳业股份有限公司 | 一种合成2’-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法与应用 |
| BR112022000394A2 (pt) | 2019-07-19 | 2022-03-03 | Inbiose Nv | Produção de fucosilactose em células hospedeiras |
| US20230348944A1 (en) | 2019-12-17 | 2023-11-02 | Inbiose N.V. | Lactose converting alpha-1,2-fucosyltransferase enzymes |
| CA3178327A1 (en) | 2019-12-18 | 2021-06-24 | Inbiose N.V. | Production of sialylated oligosaccharide in host cells |
| CA3171157A1 (en) | 2020-02-14 | 2021-08-19 | Inbiose N.V. | Production of glycosylated product in host cells |
| EP4103685A1 (en) | 2020-02-14 | 2022-12-21 | Inbiose N.V. | Glycominimized bacterial host cells |
| EP3954769A1 (en) | 2020-08-10 | 2022-02-16 | Inbiose N.V. | Production of oligosaccharide mixtures by a cell |
| EP4192945A1 (en) | 2020-08-10 | 2023-06-14 | Inbiose N.V. | Cellular production of sialylated di- and/or oligosaccharides |
| US20240368651A1 (en) | 2020-08-10 | 2024-11-07 | Inbiose N.V. | Cellular production of di- and/or oligosaccharides |
| US20240011062A1 (en) | 2020-08-10 | 2024-01-11 | Inbiose N.V. | Method to produce a purified mixture of different oligosaccharides produced by cell cultivation or microbial fermentation |
| US20250188504A1 (en) | 2020-08-10 | 2025-06-12 | Inbiose N.V. | Production of a mixture of mammalian milk oligosaccharides by a cell |
| US20240035055A1 (en) | 2020-08-10 | 2024-02-01 | Inbiose N.V. | Process for purification of an oligosaccharide solution produced by cell cultivation or microbial fermentation |
| WO2022129470A1 (en) | 2020-12-18 | 2022-06-23 | Inbiose N.V. | Variant sucrose permease polypeptides |
| EP4019534A1 (en) * | 2020-12-22 | 2022-06-29 | Chr. Hansen HMO GmbH | Microbial cells possessing a reduced lactose internalization for producing an oligosaccharide |
| CN116745430A (zh) | 2021-01-20 | 2023-09-12 | 因比奥斯公司 | 在宿主细胞中产生包含ln3作为核心结构的寡糖 |
| KR20230170961A (ko) | 2021-04-16 | 2023-12-19 | 인바이오스 엔.브이. | 시알릴화 이당 및/또는 올리고당의 세포 생산 |
| US20240294959A1 (en) | 2021-04-16 | 2024-09-05 | Inbiose N.V. | Cellular production of bioproducts |
| AU2022258873A1 (en) | 2021-04-16 | 2023-11-30 | Inbiose N.V. | Fermentative production |
| WO2023285650A1 (en) | 2021-07-16 | 2023-01-19 | Inbiose N.V. | Lacto-n-biose converting fucosyltransferases |
| CN113881586B (zh) * | 2021-09-27 | 2023-03-10 | 四川大学 | 耐高温、耐高糖、耐高盐酿酒酵母菌株及构建方法和应用 |
| KR102477273B1 (ko) * | 2021-11-24 | 2022-12-16 | (주)에이피테크놀로지 | 효소 처리를 통한 2'-푸코실락토오스의 생산성 증대 방법 |
| AU2022415533A1 (en) | 2021-12-14 | 2024-08-01 | Inbiose N.V. | Production of alpha-1,4-fucosylated compounds |
| EP4448734A1 (en) | 2021-12-14 | 2024-10-23 | Inbiose N.V. | Production of alpha-1,3-fucosylated compounds |
| CN114645070B (zh) * | 2022-02-28 | 2022-12-20 | 北京焉支山科技有限公司 | 一种己糖-6-磷酸组合物的制备方法及其在化妆品中的应用 |
| WO2024017987A1 (en) | 2022-07-20 | 2024-01-25 | Inbiose N.V. | Production of oligosaccharides in host cells |
| EP4662224A1 (en) | 2023-02-06 | 2025-12-17 | Inbiose N.V. | Production of a saccharide by a cell with reduced synthesis of lactobionic acid |
| WO2024165525A1 (en) | 2023-02-06 | 2024-08-15 | Inbiose N.V. | Production of a saccharide, lactobionic acid and/or glycosylated forms of lactobionic acid |
| CN120641559A (zh) | 2023-02-07 | 2025-09-12 | 因比奥斯公司 | 由具有降低的UDP-GlcNAc合成的细胞来产生二糖和/或乳寡糖 |
| WO2024223815A1 (en) | 2023-04-26 | 2024-10-31 | Inbiose N.V. | Production of a negatively charged oligosaccharide by a cell |
| WO2025224348A1 (en) | 2024-04-26 | 2025-10-30 | Inbiose N.V. | Production of a milk oligosaccharide mixture |
| WO2026052789A1 (en) | 2024-09-05 | 2026-03-12 | Inbiose N.V. | Fucosyltransferase mutants |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5858752A (en) | 1995-06-07 | 1999-01-12 | The General Hospital Corporation | Fucosyltransferase genes and uses thereof |
| PT1991690E (pt) * | 2006-03-09 | 2014-04-22 | Centre Nat Rech Scient | Método para a produção de oligossacáridos sialilados |
| SG186836A1 (en) * | 2010-07-12 | 2013-02-28 | Univ Gent | Metabolically engineered organisms for the production of added value bio-products |
| EP2675899A4 (en) * | 2011-02-16 | 2014-09-10 | Glycosyn LLC | BIOSYNTHESIS OF HUMAN MILK OLIGOSACCHARIDES IN MANIPULATED BACTERIA |
| EP3517631B9 (en) * | 2011-12-16 | 2023-10-04 | Inbiose N.V. | Mutant microorganisms to synthesize ph sensitive molecules and organic acids |
| CN102604849B (zh) * | 2012-03-23 | 2013-08-14 | 天津科技大学 | 一株高效利用乳糖生产燃料乙醇的酿酒酵母工程菌 |
| US20140308702A1 (en) * | 2012-03-23 | 2014-10-16 | Silab | Yeast recombinant cell capable of producing gdp-fucose |
| US9816122B2 (en) * | 2012-09-25 | 2017-11-14 | Glycom A/S | Glycoconjugate synthesis |
-
2015
- 2015-11-12 SG SG11201701585VA patent/SG11201701585VA/en unknown
- 2015-11-12 CN CN201580053480.0A patent/CN107075506B/zh active Active
- 2015-11-12 AU AU2015345073A patent/AU2015345073B9/en active Active
- 2015-11-12 KR KR1020177007250A patent/KR102579346B1/ko active Active
- 2015-11-12 CN CN202111280441.8A patent/CN114015714A/zh active Pending
- 2015-11-12 BR BR112017006295-0A patent/BR112017006295B1/pt active IP Right Grant
- 2015-11-12 ES ES15797912T patent/ES2985061T3/es active Active
- 2015-11-12 RU RU2021109889A patent/RU2021109889A/ru unknown
- 2015-11-12 US US15/505,809 patent/US10858684B2/en active Active
- 2015-11-12 WO PCT/EP2015/076449 patent/WO2016075243A1/en not_active Ceased
- 2015-11-12 EP EP15797912.1A patent/EP3218509B1/en active Active
- 2015-11-12 RU RU2017120211A patent/RU2746410C2/ru active
- 2015-11-12 CA CA2958786A patent/CA2958786A1/en active Pending
- 2015-11-12 DK DK15797912.1T patent/DK3218509T3/da active
- 2015-11-12 JP JP2017525844A patent/JP6765372B2/ja active Active
-
2020
- 2020-09-15 JP JP2020154738A patent/JP2021000116A/ja active Pending
- 2020-10-30 US US17/085,380 patent/US20210095321A1/en active Pending
-
2021
- 2021-06-17 AU AU2021204038A patent/AU2021204038B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2015345073B2 (en) | 2021-04-08 |
| JP2017534292A (ja) | 2017-11-24 |
| US20210095321A1 (en) | 2021-04-01 |
| RU2017120211A (ru) | 2018-12-14 |
| CN107075506B (zh) | 2022-06-03 |
| AU2015345073B9 (en) | 2021-04-29 |
| JP6765372B2 (ja) | 2020-10-07 |
| SG11201701585VA (en) | 2017-05-30 |
| US10858684B2 (en) | 2020-12-08 |
| AU2015345073A1 (en) | 2017-03-23 |
| RU2017120211A3 (da) | 2019-04-25 |
| AU2021204038A1 (en) | 2021-07-08 |
| EP3218509A1 (en) | 2017-09-20 |
| RU2746410C2 (ru) | 2021-04-13 |
| JP2021000116A (ja) | 2021-01-07 |
| US20180066294A1 (en) | 2018-03-08 |
| KR20170076650A (ko) | 2017-07-04 |
| EP3218509B1 (en) | 2024-02-28 |
| KR102579346B1 (ko) | 2023-09-15 |
| CA2958786A1 (en) | 2016-05-19 |
| BR112017006295B1 (pt) | 2023-10-17 |
| WO2016075243A1 (en) | 2016-05-19 |
| AU2021204038B2 (en) | 2024-03-14 |
| BR112017006295A2 (pt) | 2017-12-12 |
| ES2985061T3 (es) | 2024-11-04 |
| RU2021109889A (ru) | 2021-05-06 |
| CN114015714A (zh) | 2022-02-08 |
| CN107075506A (zh) | 2017-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK3218509T3 (da) | Mutante mikroorganismer, der er resistente over for lactose-drab | |
| CY2024015I1 (el) | Ρυθμιση δραστικοτητας συμπληρωματος | |
| ZA201605622B (en) | 1,3-benzodioxole derivative | |
| DK3377082T3 (da) | Sammensætninger, der omfatter bakteriestammer | |
| LT3230281T (lt) | 1,3-tiazol-2-ilu pakeisti benzamidai | |
| EP3142608C0 (en) | REPLACEMENT HEART VALVE | |
| LT3157916T (lt) | Heteroarilo junginiai, skirti kinazės inhibavimui | |
| MA40551A (fr) | Composés actifs envers des bromodomaines | |
| ZA201700139B (en) | Pyridone derivative having tetrahydropyranylmethyl group | |
| DK3209912T3 (da) | Forbedret ventil | |
| HRP20190107T1 (hr) | Supstituirani 2,4 diamino-kinolin kao novi antikancerogeni agens | |
| EP3209772C0 (en) | MUTATED FUCOSIDASE | |
| FR3023171B1 (fr) | Autoinjecteur. | |
| DK3166473T3 (da) | Oftalmoskoper | |
| LT3122378T (lt) | Mutantų stafilokokiniai antigenai | |
| FR3023170B1 (fr) | Autoinjecteur. | |
| LT3155004T (lt) | Vakcina, skirta asmeniui su sutrikusiu imunitetu | |
| DK3237407T3 (da) | 3,5-diaminopyrazolkinasehæmmere | |
| DE112015001647A5 (de) | Doppelkupplungsgetriebe | |
| DE112015002808A5 (de) | Ausrückvorrichtung | |
| DK3122217T3 (da) | Isoleret kande | |
| SG10201402964RA (en) | Dehydration of 2,3-butanediole | |
| HUE043314T2 (hu) | 1,3-butadién szintézis | |
| ES1114184Y (es) | Estribera Perfeccionada | |
| ES1108005Y (es) | Parrilla desmontable. |