DK3242949T3 - Crispr-cas-system til en gærværtscelle - Google Patents
Crispr-cas-system til en gærværtscelle Download PDFInfo
- Publication number
- DK3242949T3 DK3242949T3 DK16700158.5T DK16700158T DK3242949T3 DK 3242949 T3 DK3242949 T3 DK 3242949T3 DK 16700158 T DK16700158 T DK 16700158T DK 3242949 T3 DK3242949 T3 DK 3242949T3
- Authority
- DK
- Denmark
- Prior art keywords
- host
- crispr
- cas system
- host cell
- cell
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
- C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15150148 | 2015-01-06 | ||
| EP15150134 | 2015-01-06 | ||
| US201562177497P | 2015-03-16 | 2015-03-16 | |
| PCT/EP2016/050136 WO2016110512A1 (en) | 2015-01-06 | 2016-01-06 | A crispr-cas system for a yeast host cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3242949T3 true DK3242949T3 (da) | 2022-01-24 |
Family
ID=56355549
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16700158.5T DK3242949T3 (da) | 2015-01-06 | 2016-01-06 | Crispr-cas-system til en gærværtscelle |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US10619170B2 (da) |
| EP (1) | EP3242949B1 (da) |
| DK (1) | DK3242949T3 (da) |
| WO (1) | WO2016110512A1 (da) |
Families Citing this family (67)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US20150166984A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting alpha-antitrypsin point mutations |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| US11396665B2 (en) * | 2015-01-06 | 2022-07-26 | Dsm Ip Assets B.V. | CRISPR-CAS system for a filamentous fungal host cell |
| CN108064287A (zh) * | 2015-01-06 | 2018-05-22 | 帝斯曼知识产权资产管理有限公司 | 用于解脂酵母宿主细胞的crispr-cas系统 |
| SG10202104041PA (en) | 2015-10-23 | 2021-06-29 | Harvard College | Nucleobase editors and uses thereof |
| US10689670B2 (en) | 2016-06-14 | 2020-06-23 | Dsm Ip Assets B.V. | Recombinant yeast cell |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
| WO2018073107A1 (en) | 2016-10-19 | 2018-04-26 | Dsm Ip Assets B.V. | Eukaryotic cell comprising xylose isomerase |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| CN108330111B (zh) * | 2017-01-20 | 2023-01-31 | 生合万物(苏州)生物科技有限公司 | 细胞色素p450突变蛋白及其应用 |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| WO2018172328A1 (en) | 2017-03-21 | 2018-09-27 | Dsm Ip Assets B.V. | Improved glycerol free ethanol production |
| WO2018176009A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| US20200032252A1 (en) | 2017-04-06 | 2020-01-30 | Dsm Ip Assets B.V. | Self-guiding integration construct (sgic) |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| EP3684939A1 (en) | 2017-09-18 | 2020-07-29 | Amyris, Inc. | Methods for genomic integration in pichia and other host cells |
| WO2019190590A2 (en) | 2017-09-18 | 2019-10-03 | Amyris, Inc. | Methods for genomic integration for kluyveromyces host cells |
| US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| EP3790969A1 (en) | 2018-05-09 | 2021-03-17 | DSM IP Assets B.V. | Crispr transient expression construct (ctec) |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| EP3820495A4 (en) | 2018-07-09 | 2022-07-20 | The Broad Institute Inc. | RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES |
| US12203111B2 (en) | 2018-07-19 | 2025-01-21 | Xylogenics, Inc. | Materials and methods for creating strains of Saccharomyces cerevisiae that exhibit an increased ability to ferment oligosaccharides into ethanol |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US20220010305A1 (en) * | 2018-10-31 | 2022-01-13 | Novozymes A/S | Genome Editing by Guided Endonuclease and Single-stranded Oligonucleotide |
| KR102117946B1 (ko) | 2018-11-19 | 2020-06-02 | 중앙대학교 산학협력단 | 다중 삽입 영양 요구성 선별 표지 재순환용 효모 재조합벡터 시스템 |
| US20220064607A1 (en) | 2018-12-31 | 2022-03-03 | Dsm Ip Assets B.V. | Novel acetyl-transferases |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| RS64248B1 (sr) * | 2019-01-29 | 2023-06-30 | Clariant Produkte Deutschland Gmbh | Himerni promotor za upotrebu u metaboličkom inženjeringu |
| EP3942043A2 (en) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| WO2020224987A1 (en) * | 2019-05-06 | 2020-11-12 | Dsm Ip Assets B.V. | Multipartite crispr donor |
| WO2020263732A1 (en) * | 2019-06-24 | 2020-12-30 | Danisco Us Inc | Disruption of cdc42 effectors in yeast for increased alcohol and lysine production |
| US20210010006A1 (en) * | 2019-07-08 | 2021-01-14 | Inscripta, Inc. | Increased nucleic acid-guided cell editing via a lexa-rad51 fusion protein |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| BR112022022603A2 (pt) | 2020-05-08 | 2023-01-17 | Broad Inst Inc | Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla |
| WO2022003130A2 (en) | 2020-07-01 | 2022-01-06 | Dsm Ip Assets B.V. | Yeast expression system |
| EP4225929A1 (en) | 2020-10-05 | 2023-08-16 | DSM IP Assets B.V. | Saccharomyces yeast cell and fermentation process using such |
| CN114507684B (zh) * | 2020-11-16 | 2024-05-28 | 中国科学院微生物研究所 | 一种抑制地中海富盐菌中目的基因表达的方法 |
| JP7694022B2 (ja) * | 2020-12-23 | 2025-06-18 | トヨタ自動車株式会社 | 形質転換補助用プラスミド及びこれを用いた形質転換体の製造方法、並びに形質転換方法 |
| CN112852860B (zh) * | 2021-02-04 | 2023-04-28 | 天津大学 | 质粒载体及其在构建多拷贝表达系统中的应用 |
| CN113174375B (zh) * | 2021-04-23 | 2022-11-04 | 天津大学 | Aro3蛋白突变体及其应用 |
| CN113652444A (zh) * | 2021-07-06 | 2021-11-16 | 集美大学 | 一种利用基因编辑构建法夫酵母的方法 |
| EP4370688A1 (en) | 2021-07-12 | 2024-05-22 | Danisco Us Inc | Recombinant yeast cell |
| MX2024000509A (es) | 2021-07-12 | 2024-03-15 | Danisco Us Inc | Celula de levadura recombinante. |
| MX2024000511A (es) | 2021-07-12 | 2024-03-11 | Danisco Us Inc | Celula de levadura recombinante. |
| CN118234857A (zh) | 2021-11-04 | 2024-06-21 | 丹尼斯科美国公司 | 变体多肽和重组酵母细胞 |
| EP4426848A1 (en) | 2021-11-04 | 2024-09-11 | Danisco Us Inc | Process for the production of ethanol and recombinant yeast cell |
| MX2024005281A (es) | 2021-11-04 | 2024-05-17 | Danisco Us Inc | Celula de levadura recombinante. |
| WO2023164591A2 (en) * | 2022-02-23 | 2023-08-31 | Metagenomi, Inc. | Systems and methods for transposing cargo nucleotide sequences |
| EP4365192A1 (en) | 2022-11-04 | 2024-05-08 | DSM IP Assets B.V. | Microbial production of growth factors |
| IT202300006378A1 (it) | 2023-03-31 | 2024-10-01 | Univ Degli Studi Di Milano Bicocca | Procedimento per la produzione di trealosio |
| CN118389516B (zh) * | 2024-05-27 | 2024-11-29 | 江南大学 | 一种乳酸克鲁维基因组多位点编辑和高效筛选方法 |
| CN119876222A (zh) * | 2024-12-31 | 2025-04-25 | 厦门大学 | 一种酵母全基因组整合工具包及其应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CA1341226C (en) | 1988-08-16 | 2001-05-01 | Wim Van Hartingsveldt | Gene replacement as a tool for the construction of aspergillus strains |
| EP0635574B1 (en) | 1993-07-23 | 2003-04-23 | Dsm N.V. | Selection marker gene free recombinant strains, a method for obtaining them and the use of these strains |
| EP0979294B1 (en) | 1997-04-11 | 2015-05-27 | DSM IP Assets B.V. | Gene conversion as a tool for the construction of recombinant industrial filamentous fungi |
| WO2005005672A1 (en) | 2003-07-15 | 2005-01-20 | Mintek | Oxidative leach process |
| AU2004272629A1 (en) | 2003-09-12 | 2005-03-24 | Commonwealth Scientific And Industrial Research Organisation | Modified gene-silencing nucleic acid molecules and uses thereof |
| WO2005095624A2 (en) | 2004-04-02 | 2005-10-13 | Dsm Ip Assets B.V. | Filamentous fungal mutants with improved homologous recombination efficiency |
| AU2005293578B2 (en) | 2004-10-15 | 2010-10-21 | Dsm Ip Assets B.V. | Method for production of a compound in a eukaryotic cell |
| AU2006207463B2 (en) | 2005-01-24 | 2011-03-17 | Dsm Ip Assets B.V. | Method for producing a compound of interest in a filamentous fungal cell |
| EP2371967B1 (en) | 2005-03-18 | 2015-06-03 | DSM IP Assets B.V. | Production of carotenoids in oleaginous yeast and fungi |
| ES2534282T3 (es) | 2006-06-29 | 2015-04-21 | Dsm Ip Assets B.V. | Un método para lograr la expresión polipeptídica mejorada |
| WO2008053019A2 (en) | 2006-11-02 | 2008-05-08 | Dsm Ip Assets B.V. | Method for reducing the expression of a gene in a filamentous fungal cell |
| EP2421986A1 (en) | 2009-04-22 | 2012-02-29 | DSM IP Assets B.V. | Process for the production of a recombinant polypeptide of interest |
| US8889394B2 (en) | 2009-09-07 | 2014-11-18 | Empire Technology Development Llc | Multiple domain proteins |
| DK2831238T3 (da) | 2012-03-27 | 2018-04-03 | Dsm Ip Assets Bv | Kloningsfremgangsmåde |
| DK3019602T3 (da) | 2013-07-10 | 2018-11-12 | Glykos Finland Oy | Multiple protease-defekte filamentøse svampeceller og fremgangsmåder til anvendelse deraf |
| US9944925B2 (en) | 2013-08-02 | 2018-04-17 | Enevolv, Inc. | Processes and host cells for genome, pathway, and biomolecular engineering |
| AU2014368982B2 (en) * | 2013-12-19 | 2021-03-25 | Amyris, Inc. | Methods for genomic integration |
| CN108064287A (zh) * | 2015-01-06 | 2018-05-22 | 帝斯曼知识产权资产管理有限公司 | 用于解脂酵母宿主细胞的crispr-cas系统 |
| EP3320091B1 (en) * | 2015-07-06 | 2020-11-11 | DSM IP Assets B.V. | Guide rna assembly vector |
| JP2019520069A (ja) * | 2016-07-13 | 2019-07-18 | ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． | 藻類宿主細胞用のｃｒｉｓｐｒ−ｃａｓシステム |
-
2016
- 2016-01-06 EP EP16700158.5A patent/EP3242949B1/en active Active
- 2016-01-06 US US15/541,431 patent/US10619170B2/en active Active
- 2016-01-06 DK DK16700158.5T patent/DK3242949T3/da active
- 2016-01-06 WO PCT/EP2016/050136 patent/WO2016110512A1/en not_active Ceased
-
2020
- 2020-02-03 US US16/780,063 patent/US11118193B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20200172934A1 (en) | 2020-06-04 |
| US20180010151A1 (en) | 2018-01-11 |
| EP3242949A1 (en) | 2017-11-15 |
| WO2016110512A1 (en) | 2016-07-14 |
| US10619170B2 (en) | 2020-04-14 |
| US11118193B2 (en) | 2021-09-14 |
| EP3242949B1 (en) | 2021-11-03 |
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