DK3268482T3 - Fremgangsmåder til indføring af en flerhed af ekspressionskonstruktioner i en eukaryot celle - Google Patents
Fremgangsmåder til indføring af en flerhed af ekspressionskonstruktioner i en eukaryot celle Download PDFInfo
- Publication number
- DK3268482T3 DK3268482T3 DK16711089.9T DK16711089T DK3268482T3 DK 3268482 T3 DK3268482 T3 DK 3268482T3 DK 16711089 T DK16711089 T DK 16711089T DK 3268482 T3 DK3268482 T3 DK 3268482T3
- Authority
- DK
- Denmark
- Prior art keywords
- introducing
- methods
- eukaryotic cell
- expression constructs
- constructs
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562130455P | 2015-03-09 | 2015-03-09 | |
| US201562207650P | 2015-08-20 | 2015-08-20 | |
| PCT/US2016/021568 WO2016145084A1 (en) | 2015-03-09 | 2016-03-09 | Methods of introducing multiple expression constructs into a eukaryotic cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3268482T3 true DK3268482T3 (da) | 2020-03-09 |
Family
ID=55587379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16711089.9T DK3268482T3 (da) | 2015-03-09 | 2016-03-09 | Fremgangsmåder til indføring af en flerhed af ekspressionskonstruktioner i en eukaryot celle |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US10385352B2 (da) |
| EP (1) | EP3268482B1 (da) |
| CN (1) | CN107429255B (da) |
| DK (1) | DK3268482T3 (da) |
| WO (1) | WO2016145084A1 (da) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9404101B2 (en) * | 2011-08-24 | 2016-08-02 | Novozymes, Inc. | Methods for obtaining positive transformants of a filamentous fungal host cell |
| IN2014CN02136A (da) * | 2011-08-24 | 2015-05-29 | Novozymes Inc | |
| CN107429255B (zh) * | 2015-03-09 | 2022-01-25 | 诺维信公司 | 将多种表达构建体引入真核细胞的方法 |
| JP2018534921A (ja) * | 2015-10-01 | 2018-11-29 | マサチューセッツ インスティテュート オブ テクノロジー | 生物学的ステートマシン |
| US11965189B2 (en) | 2016-03-24 | 2024-04-23 | Novozymes A/S | Cellobiohydrolase variants and polynucleotides encoding same |
| US20190225674A1 (en) * | 2016-07-11 | 2019-07-25 | Massachusetts Institute Of Technology | Tools for next generation komagataella (pichia) engineering |
| JP7467119B2 (ja) * | 2017-02-17 | 2024-04-15 | ロンザ リミテッド | 発現困難タンパク質のための多部位ｓｓｉ細胞 |
| US20200231974A1 (en) * | 2017-07-18 | 2020-07-23 | Genovie Ab | A two-component vector library system for rapid assembly and diversification of full-length t-cell receptor open reading frames |
| EP3830261A1 (en) * | 2018-08-02 | 2021-06-09 | Novozymes A/S | Preparation of combinatorial libraries of dna constructs using introns |
| CN111518790B (zh) * | 2018-11-27 | 2021-11-23 | 江南大学 | 一种蔗糖水解酶突变体及其制备方法与应用 |
| CA3121271A1 (en) | 2018-11-28 | 2020-06-04 | Novozymes A/S | Modified filamentous fungal host cells |
| US12516362B2 (en) | 2018-11-28 | 2026-01-06 | Novozymes A/S | Filamentous fungal host cells |
| CN110408645B (zh) * | 2019-08-14 | 2021-01-08 | 华中科技大学 | 一种解脂耶氏酵母中目的基因多次无痕整合系统及方法 |
| EP4698669A1 (en) | 2023-04-21 | 2026-02-25 | Novozymes A/S | Generation of multi-copy host cells |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| US5989870A (en) | 1986-04-30 | 1999-11-23 | Rohm Enzyme Finland Oy | Method for cloning active promoters |
| FI964808A0 (fi) | 1994-06-03 | 1996-12-02 | Novo Nordisk Biotech Inc | Puhdistetut Myceliophthora lakkaasit ja niitä koodaavat nukleiinihapot |
| CN101659926A (zh) | 1994-06-30 | 2010-03-03 | 诺沃奇梅兹有限公司 | 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子 |
| CN100510096C (zh) | 1999-03-22 | 2009-07-08 | 诺沃奇梅兹有限公司 | 用于在真菌细胞中表达基因的启动子 |
| WO2004111228A1 (en) | 2003-05-02 | 2004-12-23 | Novozymes Inc. | Methods for producing secreted polypeptides |
| DK1682656T3 (da) | 2003-10-28 | 2013-11-18 | Novozymes Inc | Polypeptider med beta-glucosidaseaktivitet og polynukleotider, der koder for dem |
| CN1914311B (zh) | 2003-12-09 | 2011-05-04 | 诺维信股份有限公司 | 消除或减少丝状真菌菌株基因表达的方法 |
| ES2469840T3 (es) | 2004-01-30 | 2014-06-20 | Novozymes Inc. | Polip�ptidos con actividad de mejora celulol�tica y polinucle�tidos que los codifican |
| CN101516906B (zh) | 2006-07-21 | 2013-11-06 | 诺维信股份有限公司 | 提高具有生物学活性之多肽的分泌的方法 |
| CN105671027A (zh) | 2008-09-30 | 2016-06-15 | 诺维信股份有限公司 | 在丝状真菌细胞中使用阳性和阴性选择性基因的方法 |
| WO2011057083A1 (en) | 2009-11-06 | 2011-05-12 | Novozymes, Inc. | Polypeptides having xylanase activity and polynucleotides encoding same |
| EP2496694B1 (en) | 2009-11-06 | 2017-04-19 | Novozymes, Inc. | Compositions for saccharification of cellulosic material |
| DK2513290T3 (da) | 2009-12-18 | 2016-01-25 | Novozymes Inc | Fremgangsmåder til fremstilling af polypeptider i Trichoderma-mutanter med proteasemangel |
| CN103261420B (zh) | 2010-12-16 | 2016-01-20 | 诺维信股份有限公司 | 用于在真菌细胞中表达基因的启动子 |
| FR2972599B1 (fr) | 2011-03-08 | 2014-02-14 | Inst Polytechnique Grenoble | Systeme electrique comportant une pompe electromagnetique servant au refroidissement d'un circuit electrique du systeme |
| EP2527448A1 (en) | 2011-05-23 | 2012-11-28 | Novozymes A/S | Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi |
| US9663772B2 (en) | 2011-08-24 | 2017-05-30 | Novozymes, Inc. | Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof |
| CN103890165A (zh) | 2011-08-24 | 2014-06-25 | 诺维信股份有限公司 | 纤维素分解酶组合物及其用途 |
| IN2014CN02136A (da) | 2011-08-24 | 2015-05-29 | Novozymes Inc | |
| CN107429255B (zh) * | 2015-03-09 | 2022-01-25 | 诺维信公司 | 将多种表达构建体引入真核细胞的方法 |
-
2016
- 2016-03-09 CN CN201680014407.7A patent/CN107429255B/zh active Active
- 2016-03-09 DK DK16711089.9T patent/DK3268482T3/da active
- 2016-03-09 US US15/556,439 patent/US10385352B2/en active Active
- 2016-03-09 WO PCT/US2016/021568 patent/WO2016145084A1/en not_active Ceased
- 2016-03-09 EP EP16711089.9A patent/EP3268482B1/en active Active
-
2019
- 2019-07-09 US US16/506,201 patent/US11840696B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20180037897A1 (en) | 2018-02-08 |
| WO2016145084A1 (en) | 2016-09-15 |
| CN107429255A (zh) | 2017-12-01 |
| US20190330644A1 (en) | 2019-10-31 |
| EP3268482A1 (en) | 2018-01-17 |
| US10385352B2 (en) | 2019-08-20 |
| CN107429255B (zh) | 2022-01-25 |
| US11840696B2 (en) | 2023-12-12 |
| EP3268482B1 (en) | 2019-12-11 |
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