DK3301208T3 - Reduceret codon-mutagenese - Google Patents

Reduceret codon-mutagenese Download PDF

Info

Publication number
DK3301208T3
DK3301208T3 DK17190712.4T DK17190712T DK3301208T3 DK 3301208 T3 DK3301208 T3 DK 3301208T3 DK 17190712 T DK17190712 T DK 17190712T DK 3301208 T3 DK3301208 T3 DK 3301208T3
Authority
DK
Denmark
Prior art keywords
codon mutagenesis
reduced codon
reduced
mutagenesis
codon
Prior art date
Application number
DK17190712.4T
Other languages
English (en)
Inventor
Richard Fox
Lorraine J Giver
Daniel Held
Douglas Hattendorf
Trish Choudhary
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2009/057507 external-priority patent/WO2010144103A1/en
Priority claimed from US12/562,988 external-priority patent/US8383346B2/en
Application filed by Codexis Inc filed Critical Codexis Inc
Application granted granted Critical
Publication of DK3301208T3 publication Critical patent/DK3301208T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK17190712.4T 2009-09-18 2010-09-17 Reduceret codon-mutagenese DK3301208T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
PCT/US2009/057507 WO2010144103A1 (en) 2009-06-11 2009-09-18 Combined automated parallel synthesis of polynucleotide variants
US12/562,988 US8383346B2 (en) 2008-06-13 2009-09-18 Combined automated parallel synthesis of polynucleotide variants
US28387709P 2009-12-09 2009-12-09
EP10817881.5A EP2478137B1 (en) 2009-09-18 2010-09-17 Reduced codon mutagenesis

Publications (1)

Publication Number Publication Date
DK3301208T3 true DK3301208T3 (da) 2020-12-14

Family

ID=43759019

Family Applications (2)

Application Number Title Priority Date Filing Date
DK17190712.4T DK3301208T3 (da) 2009-09-18 2010-09-17 Reduceret codon-mutagenese
DK10817881.5T DK2478137T3 (da) 2009-09-18 2010-09-17 Reduceret kodonmutagenese

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK10817881.5T DK2478137T3 (da) 2009-09-18 2010-09-17 Reduceret kodonmutagenese

Country Status (5)

Country Link
EP (2) EP3301208B1 (da)
DK (2) DK3301208T3 (da)
HU (2) HUE037123T2 (da)
SG (1) SG179197A1 (da)
WO (1) WO2011035105A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017213758A1 (en) 2016-06-09 2017-12-14 Codexis, Inc. Biocatalysts and methods for hydroxylation of chemical compounds
MX2018015534A (es) 2016-06-15 2019-03-14 Codexis Inc Beta-glucosidasas dise?adas y metodos de glucosilacion.
IL264686B2 (en) 2016-08-26 2024-07-01 Codexis Inc Engineered imine reductases and methods for reversible amination of ketone and amine compounds
WO2021136194A1 (zh) * 2019-12-30 2021-07-08 南京金斯瑞生物科技有限公司 构建基因突变文库的方法
WO2025212767A1 (en) * 2024-04-02 2025-10-09 Watchmaker Genomics, Inc. Gene variant libraries and methods of use thereof

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
FI104465B (fi) 1995-06-14 2000-02-15 Valio Oy Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6537746B2 (en) 1997-12-08 2003-03-25 Maxygen, Inc. Method for creating polynucleotide and polypeptide sequences
JP4221100B2 (ja) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 半導体装置
ES2341217T3 (es) 1999-01-19 2010-06-17 Maxygen, Inc. Recombinacion de acidos nucleicos mediada por oligonucleotidos.
EP1272967A2 (en) 2000-03-30 2003-01-08 Maxygen, Inc. In silico cross-over site selection
JP2004508011A (ja) 2000-04-03 2004-03-18 マキシジェン, インコーポレイテッド スブチリシン変異体
JP2007502124A (ja) 2003-08-11 2007-02-08 コデクシス, インコーポレイテッド 改良ケトレダクターゼポリペプチドおよび関連ポリヌクレオチド
CA2535255A1 (en) 2003-08-11 2005-02-24 Codexis, Inc. Improved halohydrin dehalogenases and related polynucleotides
JP2009519011A (ja) * 2005-12-01 2009-05-14 ドマンティス リミテッド インターロイキン1受容体1型に結合する非競合ドメイン抗体フォーマット
US20090075378A1 (en) * 2007-02-20 2009-03-19 Anaptysbio, Inc. Somatic hypermutation systems
AU2008347218B2 (en) * 2007-12-31 2015-02-05 Xoma Technology Ltd. Methods and materials for targeted mutagenesis
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants

Also Published As

Publication number Publication date
HUE037123T2 (hu) 2018-08-28
EP2478137A4 (en) 2013-10-23
EP2478137B1 (en) 2017-09-13
EP3301208B1 (en) 2020-11-04
EP3301208A1 (en) 2018-04-04
SG179197A1 (en) 2012-04-27
WO2011035105A1 (en) 2011-03-24
HUE051913T2 (hu) 2021-04-28
DK2478137T3 (da) 2017-11-27
EP2478137A1 (en) 2012-07-25

Similar Documents

Publication Publication Date Title
EP2515984A4 (en) INTERFACE
DE10767394T8 (de) Kindertrage
DK2411442T4 (da) Umættet polyesterresin
DE112010004215A5 (de) Hydrostataktor
BRPI1008782A2 (pt) primidinas fundidas
BRPI1008827A2 (pt) deisobutenizador
BR112012000657A2 (pt) novos azabicilohexanos
BRPI1014968A2 (pt) subalargador
DE112010002728A5 (de) Turboinhalator
EP2499707A4 (en) INTERFACE
DE112010004249A5 (de) Spindelaktor
DE112009005323A5 (de) Hebelarmprüfmaschine
EP2415046C0 (en) SHOULDER BRACKET
BRPI1008070A2 (pt) tricianoboratos
BR112012004964A2 (pt) girocóptero
DK2483126T3 (da) Skinnekøretøj
DE112010004865T8 (de) Lamellennassreibkupplung
EP2512583C0 (en) SHEATH
DK2506968T3 (da) Faujasit-zeolit
DE112010003255A5 (de) Fördergutverteiler
BRPI1010159A2 (pt) bistuti
EP2398476A4 (en) Policosanol nanoparticles
EP2518828A4 (en) Rfid tag
ES1070429Y (es) Traspaleta mejorada
DK3301208T3 (da) Reduceret codon-mutagenese