DK3310909T3 - Sammensætninger og fremgangsmåder til at føre proteiner til specifikke loci i genomet - Google Patents

Sammensætninger og fremgangsmåder til at føre proteiner til specifikke loci i genomet Download PDF

Info

Publication number
DK3310909T3
DK3310909T3 DK16733813.6T DK16733813T DK3310909T3 DK 3310909 T3 DK3310909 T3 DK 3310909T3 DK 16733813 T DK16733813 T DK 16733813T DK 3310909 T3 DK3310909 T3 DK 3310909T3
Authority
DK
Denmark
Prior art keywords
locis
genome
compositions
methods
specific
Prior art date
Application number
DK16733813.6T
Other languages
English (en)
Inventor
Eric Ostertag
Tseten Yeshi
Xianghong Li
Original Assignee
Poseida Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Poseida Therapeutics Inc filed Critical Poseida Therapeutics Inc
Application granted granted Critical
Publication of DK3310909T3 publication Critical patent/DK3310909T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/21Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21004Type II site-specific deoxyribonuclease (3.1.21.4)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK16733813.6T 2015-06-17 2016-06-16 Sammensætninger og fremgangsmåder til at føre proteiner til specifikke loci i genomet DK3310909T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562181162P 2015-06-17 2015-06-17
PCT/US2016/037922 WO2016205554A1 (en) 2015-06-17 2016-06-16 Compositions and methods for directing proteins to specific loci in the genome

Publications (1)

Publication Number Publication Date
DK3310909T3 true DK3310909T3 (da) 2021-09-13

Family

ID=56292934

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16733813.6T DK3310909T3 (da) 2015-06-17 2016-06-16 Sammensætninger og fremgangsmåder til at føre proteiner til specifikke loci i genomet

Country Status (11)

Country Link
US (2) US11473082B2 (da)
EP (2) EP3929286A1 (da)
JP (3) JP2018521689A (da)
KR (1) KR102637402B1 (da)
CN (2) CN121975771A (da)
AU (2) AU2016278226B2 (da)
CA (1) CA2989827C (da)
DK (1) DK3310909T3 (da)
ES (1) ES2886599T3 (da)
PT (1) PT3310909T (da)
WO (1) WO2016205554A1 (da)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2013344375B2 (en) 2012-11-16 2017-09-14 Transposagen Biopharmaceuticals, Inc. Site-specific enzymes and methods of use
IL287561B2 (en) 2014-10-01 2024-03-01 Massachusetts Gen Hospital Methods for increasing efficiency of nuclease-induced homology-directed repair
CA3105873A1 (en) * 2016-09-30 2018-04-05 Poseida Therapeutics, Inc. Modified stem cell memory t cells, methods of making and methods of using same
US20190119636A1 (en) 2017-10-23 2019-04-25 Poseida Therapeutics, Inc. Modified stem cell memory t cells, methods of making and methods of using same
KR102523318B1 (ko) 2016-12-16 2023-04-18 비-모젠 바이오테크놀로지스, 인크. 증진된 hAT 패밀리 트랜스포존 매개 유전자 전달 및 연관된 조성물, 시스템, 및 방법
US11278570B2 (en) 2016-12-16 2022-03-22 B-Mogen Biotechnologies, Inc. Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
IL269203B2 (en) 2017-03-13 2024-09-01 Poseida Therapeutics Inc Compositions and methods for selective elimination and replacement of hematopoietic stem cells
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
KR20240046316A (ko) * 2017-07-11 2024-04-08 시그마-알드리치 컴퍼니., 엘엘씨 표적된 게놈 변형을 개선하기 위한 뉴클레오솜 상호작용 단백질 도메인 사용
WO2019135816A2 (en) * 2017-10-23 2019-07-11 The Broad Institute, Inc. Novel nucleic acid modifiers
US10329543B2 (en) 2017-10-23 2019-06-25 Poseida Therapeutics, Inc. Modified stem cell memory T cells, methods of making and methods of using same
WO2019126578A1 (en) * 2017-12-20 2019-06-27 Poseida Therapeutics, Inc. Compositions and methods for directing proteins to specific loci in the genome
JP2021527427A (ja) 2018-06-21 2021-10-14 ビー−モーゲン・バイオテクノロジーズ,インコーポレーテッド 強化されたhATファミリーのトランスポゾンが介在する遺伝子導入ならびに関連する組成物、システム、及び方法
CN110938658B (zh) * 2018-09-21 2023-02-07 中国科学院分子细胞科学卓越创新中心 一种抗体进化方法及其应用
WO2020077360A1 (en) 2018-10-12 2020-04-16 Poseida Therapeutics, Inc. Hematopoietic stem cell compositions, methods of making and method of use
CN113498439A (zh) 2018-12-20 2021-10-12 波赛达治疗公司 纳米转座子组合物及使用方法
WO2020160481A1 (en) * 2019-02-01 2020-08-06 The General Hospital Corporation Targetable 3'-overhang nuclease fusion proteins
EP3954699A4 (en) * 2019-04-09 2023-05-10 Japan Science and Technology Agency Nucleic acid binding protein
CN114258398A (zh) 2019-06-13 2022-03-29 总医院公司 工程化的人内源性病毒样颗粒及使用其递送至细胞的方法
WO2021046261A1 (en) 2019-09-05 2021-03-11 Poseida Therapeutics, Inc. Allogeneic cell compositions and methods of use
AU2020407235A1 (en) 2019-12-20 2022-07-07 Poseida Therapeutics, Inc. Anti-MUC1 compositions and methods of use
WO2021183795A1 (en) 2020-03-11 2021-09-16 Poseida Therapeutics, Inc. Chimeric stimulatory receptors and methods of use in t cell activation and differentiation
CN116670154A (zh) 2020-07-24 2023-08-29 总医院公司 增强的病毒样颗粒及使用其递送至细胞的方法
EP4231999B1 (en) 2020-10-21 2025-03-05 Poseida Therapeutics, Inc. Compositions and methods for delivery of nucleic acids
WO2022087354A1 (en) * 2020-10-23 2022-04-28 Altius Institute For Biomedical Sciences Cell permeable proteins for genome engineering
CN112750498B (zh) * 2020-12-30 2022-06-24 同济大学 靶向逆转录引物结合位点从而抑制hiv病毒复制的方法
CA3208993A1 (en) 2021-02-23 2022-09-01 Joshua RYCHAK Compositions and methods for delivery of nucleic acids
US20240060090A1 (en) 2021-02-23 2024-02-22 Poseida Therapeutics, Inc. Genetically modified induced pluripotent stem cells and methods of use thereof
WO2023039488A1 (en) 2021-09-09 2023-03-16 Iovance Biotherapeutics, Inc. Processes for generating til products using pd-1 talen knockdown
US20240401085A1 (en) 2021-10-04 2024-12-05 Poseida Therapeutics, Inc. Transposon compositions and methods of use thereof
AU2023210287A1 (en) 2022-01-21 2024-09-05 Poseida Therapeutics, Inc. Compositions and methods for delivery of nucleic acids
EP4482967A1 (en) 2022-02-23 2025-01-01 Poseida Therapeutics, Inc. Genetically modified cells and methods of use thereof
WO2023209649A1 (en) * 2022-04-29 2023-11-02 Seqirus, Inc. Method of dna linearization
US20250388883A1 (en) * 2022-06-30 2025-12-25 Demeetra Agbio, Inc. Gene editing compositions and methods of use thereof
EP4605525A1 (en) 2022-10-21 2025-08-27 Keygene N.V. Rna transfection in plant cells with modified rna
WO2024112711A2 (en) 2022-11-21 2024-05-30 Iovance Biotherapeutics, Inc. Methods for assessing proliferation potency of gene-edited t cells
KR20250122544A (ko) 2022-11-21 2025-08-13 이오반스 바이오테라퓨틱스, 인크. 종양 침윤 림프구의 확장을 위한 2차원 공정 및 이를 통한 요법
WO2024118836A1 (en) 2022-11-30 2024-06-06 Iovance Biotherapeutics, Inc. Processes for production of tumor infiltrating lymphocytes with shortened rep step
WO2024121354A1 (en) 2022-12-08 2024-06-13 Keygene N.V. Duplex sequencing with covalently closed dna ends
JP2026503550A (ja) 2023-01-20 2026-01-29 ポセイダ セラピューティクス,インコーポレイティド リピドイド化合物ならびに関連する組成物および使用
WO2024155931A1 (en) 2023-01-20 2024-07-25 Poseida Therapeutics, Inc. Lipidoid compounds and related compositions and uses
JP2026507731A (ja) * 2023-02-21 2026-03-04 ポセイダ セラピューティクス,インコーポレイティド ゲノム編集のための組成物及び方法
JP2026507648A (ja) * 2023-02-21 2026-03-04 ポセイダ セラピューティクス,インコーポレイティド ゲノム編集のための組成物及び方法
EP4689157A1 (en) 2023-04-04 2026-02-11 Keygene N.V. Linkers for duplex sequencing
IL324536A (en) 2023-05-17 2026-01-01 Poseida Therapeutics Inc HBG1 and HBG2-targeting compounds and methods for using them
CN122055354A (zh) 2023-09-01 2026-05-15 波西达治疗公司 类脂质化合物及相关组合物和用途
WO2025096980A1 (en) * 2023-11-02 2025-05-08 Poseida Therapeutics, Inc. Compositions targeting klkb1 and methods of use thereof
WO2025160432A1 (en) 2024-01-26 2025-07-31 Poseida Therapeutics, Inc. Methods and compositions for enhancing in vivo persistence and potency of cell therapy
WO2025171237A1 (en) 2024-02-08 2025-08-14 Poseida Therapeutics, Inc. Lipidoid compounds and related compositions and uses
WO2025221789A1 (en) 2024-04-16 2025-10-23 Poseida Therapeutics, Inc. Compositions and methods for use in car/tcr cell therapies
WO2026030277A1 (en) 2024-08-01 2026-02-05 Amgen Inc. Method for reducing protease activities
WO2026080393A1 (en) 2024-10-07 2026-04-16 Poseida Therapeutics, Inc. Compositions and methods for use in car cell therapies

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554101A (en) 1981-01-09 1985-11-19 New York Blood Center, Inc. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
US5672344A (en) 1987-12-30 1997-09-30 The Regents Of The University Of Michigan Viral-mediated gene transfer system
US5399346A (en) 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
US5240846A (en) 1989-08-22 1993-08-31 The Regents Of The University Of Michigan Gene therapy vector for cystic fibrosis
US5436146A (en) 1989-09-07 1995-07-25 The Trustees Of Princeton University Helper-free stocks of recombinant adeno-associated virus vectors
US5670488A (en) 1992-12-03 1997-09-23 Genzyme Corporation Adenovirus vector for gene therapy
US5529774A (en) 1991-08-13 1996-06-25 The Regents Of The University Of California In vivo transfer of the HSV-TK gene implanted retroviral producer cells
NZ244306A (en) 1991-09-30 1995-07-26 Boehringer Ingelheim Int Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation
TW261517B (da) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5911983A (en) 1992-06-26 1999-06-15 University Of Pittsburgh Gene therapy for Gaucher disease using retroviral vectors
ATE301201T1 (de) 1993-06-07 2005-08-15 Vical Inc Für die gentherapie verwendbare plasmide
US5645829A (en) 1993-06-18 1997-07-08 Beth Israel Hospital Association Mesothelial cell gene therapy
US5656465A (en) 1994-05-04 1997-08-12 Therion Biologics Corporation Methods of in vivo gene delivery
US5714353A (en) 1994-05-24 1998-02-03 Research Corporation Technologies, Inc. Safe vectors for gene therapy
US5639642A (en) 1994-06-16 1997-06-17 Novo Nordisk A/S Synthetic leader peptide sequences
US7285416B2 (en) 2000-01-24 2007-10-23 Gendaq Limited Regulated gene expression in plants
GB9506466D0 (en) 1994-08-26 1995-05-17 Prolifix Ltd Cell cycle regulated repressor and dna element
DE19524720A1 (de) 1995-07-12 1997-01-16 Hoechst Ag Zellspezifische Gentherapie mit Hilfe eines neuen Promotors für den "Tissue Inhibitor of Metalloproteinasn-3"
JP4216350B2 (ja) 1994-09-19 2009-01-28 大日本住友製薬株式会社 動物細胞感染用の組換えdnaウイルスベクター
US5789147A (en) 1994-12-05 1998-08-04 New York Blood Center, Inc. Method for concentrating white cells from whole blood by adding a red cell sedimentation reagent to whole anticoagulated blood
US5869040A (en) 1995-06-07 1999-02-09 Biogen, Inc Gene therapy methods and compositions
US5928914A (en) 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
WO2000002996A2 (en) 1998-07-10 2000-01-20 Cornell Research Foundation, Inc. Recombinant constructs and systems for secretion of proteins via type iii secretion systems
CA2246005A1 (en) 1998-10-01 2000-04-01 Hsc Research And Development Limited Partnership Hybrid genes for gene therapy in erythroid cells
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7013219B2 (en) 1999-01-12 2006-03-14 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7070934B2 (en) 1999-01-12 2006-07-04 Sangamo Biosciences, Inc. Ligand-controlled regulation of endogenous gene expression
CA2374476A1 (en) 1999-05-25 2000-11-30 Panorama Research, Inc. Interaction-activated proteins
ATE353361T1 (de) 2000-04-28 2007-02-15 Sangamo Biosciences Inc Gezielten modifikation der chromatinstruktur
CA2435394C (en) 2001-01-22 2018-01-09 Sangamo Biosciences, Inc. Modified zinc finger binding proteins
US7700754B1 (en) * 2001-06-05 2010-04-20 Masahiro Hiraoka Polypeptide for unstabilizing protein in cells under aerobic conditions and DNA encoding the same
US20030138850A1 (en) 2001-10-18 2003-07-24 Ekkehard Mossner Selecting for antibody-antigen interactions in bacteria cells by employing a protein fragment complementation assay
US7262054B2 (en) 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
WO2009095742A1 (en) 2008-01-31 2009-08-06 Cellectis New i-crei derived single-chain meganuclease and uses thereof
US20030232410A1 (en) 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
US7361635B2 (en) 2002-08-29 2008-04-22 Sangamo Biosciences, Inc. Simultaneous modulation of multiple genes
GB0226729D0 (en) 2002-11-15 2002-12-24 Medical Res Council Intracellular antibodies
US8021849B2 (en) 2004-11-05 2011-09-20 Siemens Healthcare Diagnostics Inc. Methods and kits for the determination of sirolimus in a sample
US20060252140A1 (en) 2005-04-29 2006-11-09 Yant Stephen R Development of a transposon system for site-specific DNA integration in mammalian cells
US7790379B2 (en) 2005-05-19 2010-09-07 Universite De Geneve Mapping of proteins along chromatin by chromatin cleavage
WO2007060495A1 (en) 2005-10-25 2007-05-31 Cellectis I-crei homing endonuclease variants having novel cleavage specificity and use thereof
JP2007159512A (ja) 2005-12-15 2007-06-28 Institute Of Physical & Chemical Research Iis型制限酵素を用いる翻訳終止コドンの除去方法
US20100015661A1 (en) 2006-04-21 2010-01-21 Mab-Factory Gmbh Antibody-rnase-conjugate
WO2008130629A2 (en) 2007-04-19 2008-10-30 Codon Devices, Inc. Engineered nucleases and their uses for nucleic acid assembly
EP2188372A4 (en) 2007-09-17 2011-03-02 Affomix Corp INCREASING THE SPECIFICITY OF SCFV SCREENING USING TWO BAIT REPORTERS
US20110305692A1 (en) 2009-02-24 2011-12-15 Glaxo Group Limited Antigen-binding contructs
RU2011137030A (ru) 2009-03-11 2013-04-20 УАЙТ ЭлЭлСи Способы очистки малых модулярных иммунофармацевтических белков
US8889394B2 (en) 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
EP3156062A1 (en) 2010-05-17 2017-04-19 Sangamo BioSciences, Inc. Novel dna-binding proteins and uses thereof
WO2012093833A2 (en) * 2011-01-03 2012-07-12 Toolgen Incorporation Genome engineering via designed tal effector nucleases
EP2661446B1 (en) 2011-01-06 2015-09-30 Complix SA Alphabody libraries and methods for producing the same
US9499592B2 (en) 2011-01-26 2016-11-22 President And Fellows Of Harvard College Transcription activator-like effectors
JP5996630B2 (ja) 2011-04-05 2016-09-21 セレクティスCellectis コンパクトtale−ヌクレアーゼを作製する方法及びその使用
EP2522726A1 (en) 2011-05-12 2012-11-14 Fundació Privada Centre de Regulació Genòmica (CRG) Zinc finger nucleases for p53 editing
WO2012158986A2 (en) 2011-05-17 2012-11-22 Transposagen Biopharmaceuticals, Inc. Methods for site-specific genetic modification in stem cells using xanthomonas tal nucleases (xtn) for the creation of model organisms
ES2709216T3 (es) * 2011-06-07 2019-04-15 Helmholtz Zentrum Muenchen Deutsches Forschungszentrum Gesundheit & Umwelt Gmbh Proteínas que tienen actividad nucleasa, proteínas de fusión y usos de estas
US20140304847A1 (en) 2011-06-07 2014-10-09 Ralf Kühn Recombination efficiency by inhibition of nhej dna repair
EP2790708B1 (en) 2011-12-16 2024-10-23 Targetgene Biotechnologies Ltd. Compositions and methods for modifying a predetermined target nucleic acid sequence
EP2834357B1 (en) 2012-04-04 2017-12-27 Life Technologies Corporation Tal-effector assembly platform, customized services, kits and assays
JP2015519344A (ja) 2012-05-21 2015-07-09 マサチューセッツ インスティテュート オブ テクノロジー 炭疽菌防御抗原ポアを通しての非天然化学実体のトランスロケーション
LT3401400T (lt) * 2012-05-25 2019-06-10 The Regents Of The University Of California Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui
US9181535B2 (en) * 2012-09-24 2015-11-10 The Chinese University Of Hong Kong Transcription activator-like effector nucleases (TALENs)
AU2013336601B2 (en) 2012-10-26 2018-01-25 Vrije Universiteit Brussel Vector for liver-directed gene therapy of hemophilia and methods and use thereof
AU2013344375B2 (en) 2012-11-16 2017-09-14 Transposagen Biopharmaceuticals, Inc. Site-specific enzymes and methods of use
EP3135765A1 (en) * 2012-12-06 2017-03-01 Sigma-Aldrich Co. LLC Crispr-based genome modification and regulation
EP2931898B1 (en) 2012-12-12 2016-03-09 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
US20150044772A1 (en) * 2013-08-09 2015-02-12 Sage Labs, Inc. Crispr/cas system-based novel fusion protein and its applications in genome editing
EP3115457B1 (en) 2014-03-05 2019-10-02 National University Corporation Kobe University Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
WO2015195803A1 (en) 2014-06-17 2015-12-23 Poseida Therapeutics, Inc. Methods and compositions for in vivo non-covalent linking
JP2017518082A (ja) * 2014-06-17 2017-07-06 ポセイダ セラピューティクス, インコーポレイテッド ゲノム中の特異的遺伝子座にタンパク質を指向させるための方法およびその使用
CN104450785A (zh) * 2014-12-08 2015-03-25 复旦大学 使用编码靶向核酸内切酶附着体载体的基因组编辑方法及试剂盒

Also Published As

Publication number Publication date
CN121975771A (zh) 2026-05-05
EP3310909B1 (en) 2021-06-09
HK1253508A1 (zh) 2019-06-21
CA2989827C (en) 2024-09-10
JP2021112211A (ja) 2021-08-05
KR102637402B1 (ko) 2024-02-15
US20230257737A1 (en) 2023-08-17
AU2021250992B2 (en) 2023-09-28
US20180187185A1 (en) 2018-07-05
US11473082B2 (en) 2022-10-18
AU2016278226A1 (en) 2018-01-25
EP3929286A1 (en) 2021-12-29
CN108026519A (zh) 2018-05-11
CA2989827A1 (en) 2016-12-22
JP2021182942A (ja) 2021-12-02
AU2021250992A1 (en) 2021-11-11
KR20180036696A (ko) 2018-04-09
ES2886599T3 (es) 2021-12-20
EP3310909A1 (en) 2018-04-25
WO2016205554A1 (en) 2016-12-22
JP7170090B2 (ja) 2022-11-11
AU2016278226B2 (en) 2021-08-12
JP2018521689A (ja) 2018-08-09
PT3310909T (pt) 2021-09-09

Similar Documents

Publication Publication Date Title
PT3310909T (pt) Composições e métodos para dirigir proteínas para loci específicos no genoma
DK3115457T3 (da) Fremgangsmåde til modificering af genomsekvens til specifikt at konvertere nukleinsyrebaser af målrettet dna-sekvens og molekylært kompleks til anvendelse i samme
DK3325669T3 (da) Sammensætninger og fremgangsmåder til RNA-analyse
DK3274430T3 (da) Mikroalgesammensætninger og deres anvendelser
EP3313989C0 (en) MODIFIED CRISPR RNA AND MODIFIED SINGLE CRISPR RNA AND USES THEREOF
DK3307872T3 (da) Fremgangsmåder og sammensætninger til effektiv indgivelse af nukleinsyrer og rna-baserede antimikrober
IL254000B (en) Nucleic acid sequence assembly
DK3071696T3 (da) C/ebp alfa kort aktiverings-rna-sammensætninger og fremgangsmåder til anvendelse
DK3080274T3 (da) Fremgangsmåder og sammensætninger til genom-manipulation
PL3386534T3 (pl) Kompozycje i sposoby internalizacji enzymów
KR20180084891A (ko) 구조 조성물 및 방법
DK3234133T3 (da) Crispr-baserede sammensætninger og fremgangsmåder til anvendelse
DK3386484T3 (da) Sammensætninger og fremgangsmåder til afgivelse af terapeutiske midler
DK3108009T3 (da) Fremgangsmåder og sammensætninger til DNA-profilering
DK3221363T3 (da) Antistoffer mod cd73 og anvendelser deraf
DK3495023T3 (da) Semifluorerede forbindelser og sammensætninger deraf
DK3316909T3 (da) Anti-ntb-a-antistoffer og relaterede sammensætninger og fremgangsmåder
DK3145493T3 (da) Fremgangsmåder og sammensætninger vedrørende exosomer
DK3157571T3 (da) Fedtsyrer og deres anvendelse i konjugation med biomolekyler
DK3354661T3 (da) Fuldstændigt humant antistof mod human CD137 og anvendelse deraf
DK3240895T3 (da) Sammensætninger og fremgangsmåder til proteinglykosylering
DK3357513T3 (da) Farmaceutisk sammensætning og anvendelse deraf
DK3018183T3 (da) Halogenolefinbaseret sammensætning og anvendelse deraf
DK3371313T3 (da) Fremgangsmåder og sammensætninger til forbedret plantetransformation
DK3242957T3 (da) Bakterieholdige sammensætninger og fremgangsmåder til anvendelse af samme til behandling og/eller forebyggelse af gastrointestinale, metaboliske og/eller andre sygdomme