DK344884A - Fremgangsmaade til fremstilling af modne proteiner ud fra fusionsproteiner, syntetiseret i pro- eller eukaryote celler - Google Patents

Fremgangsmaade til fremstilling af modne proteiner ud fra fusionsproteiner, syntetiseret i pro- eller eukaryote celler Download PDF

Info

Publication number: DK344884A
Authority: DK; Denmark
Prior art keywords: pro; amino acid; proteins; protein; synthetized
Prior art date: 1982-12-10

Application number

DK344884A

Other languages

English (en)

Other versions

DK344884D0 (da

Inventor

Thorkild Christensen

Per Balschmidt

Hans-Henrik Marstrand Dahl

Kim Ry Hejnaes

Original Assignee

Nordisk Insulinlab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1982-12-10

Filing date

1984-07-13

Publication date

1984-07-13

1984-07-13 Application filed by Nordisk Insulinlab filed Critical Nordisk Insulinlab

1984-07-13 Priority to DK344884A priority Critical patent/DK344884A/da

1984-07-13 Publication of DK344884D0 publication Critical patent/DK344884D0/da

1984-07-13 Publication of DK344884A publication Critical patent/DK344884A/da

Links

102000004169 proteins and genes Human genes 0.000 title abstract 5
108090000623 proteins and genes Proteins 0.000 title abstract 5
102000037865 fusion proteins Human genes 0.000 title abstract 2
108020001507 fusion proteins Proteins 0.000 title abstract 2
238000000034 method Methods 0.000 title abstract 2
238000004519 manufacturing process Methods 0.000 title 1
150000001413 amino acids Chemical class 0.000 abstract 3
102000004400 Aminopeptidases Human genes 0.000 abstract 2
108090000915 Aminopeptidases Proteins 0.000 abstract 2
238000003776 cleavage reaction Methods 0.000 abstract 2
230000007017 scission Effects 0.000 abstract 2
102000002265 Human Growth Hormone Human genes 0.000 abstract 1
108010000521 Human Growth Hormone Proteins 0.000 abstract 1
239000000854 Human Growth Hormone Substances 0.000 abstract 1
102000002704 Leucyl aminopeptidase Human genes 0.000 abstract 1
108010004098 Leucyl aminopeptidase Proteins 0.000 abstract 1
PYUSHNKNPOHWEZ-YFKPBYRVSA-N N-formyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC=O PYUSHNKNPOHWEZ-YFKPBYRVSA-N 0.000 abstract 1
108010076181 Proinsulin Proteins 0.000 abstract 1
125000003275 alpha amino acid group Chemical group 0.000 abstract 1
125000000539 amino acid group Chemical group 0.000 abstract 1
230000002255 enzymatic effect Effects 0.000 abstract 1
210000003527 eukaryotic cell Anatomy 0.000 abstract 1
238000010353 genetic engineering Methods 0.000 abstract 1
238000000338 in vitro Methods 0.000 abstract 1
125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 abstract 1
210000001236 prokaryotic cell Anatomy 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/545—IL-1
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Microbiology (AREA)
Endocrinology (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Diabetes (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

DK344884A 1982-12-10 1984-07-13 Fremgangsmaade til fremstilling af modne proteiner ud fra fusionsproteiner, syntetiseret i pro- eller eukaryote celler DK344884A (da)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
DK344884A DK344884A (da)	1982-12-10	1984-07-13	Fremgangsmaade til fremstilling af modne proteiner ud fra fusionsproteiner, syntetiseret i pro- eller eukaryote celler

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
DK549382		1982-12-10
PCT/DK1983/000118 WO1984002351A1 (en)	1982-12-10	1983-12-09	A process for preparing ripe proteins from fusion proteins, synthetized in pro- or eukaryotic cells
DK344884A DK344884A (da)	1982-12-10	1984-07-13	Fremgangsmaade til fremstilling af modne proteiner ud fra fusionsproteiner, syntetiseret i pro- eller eukaryote celler

Publications (2)

Publication Number	Publication Date
DK344884D0 DK344884D0 (da)	1984-07-13
DK344884A true DK344884A (da)	1984-07-13

Family

ID=8142905

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK344884A DK344884A (da)	1982-12-10	1984-07-13	Fremgangsmaade til fremstilling af modne proteiner ud fra fusionsproteiner, syntetiseret i pro- eller eukaryote celler

Country Status (7)

Country	Link
EP (1)	EP0127658B1 (da)
JP (1)	JPS60500043A (da)
AT (1)	ATE30246T1 (da)
AU (1)	AU2331784A (da)
DE (1)	DE3374070D1 (da)
DK (1)	DK344884A (da)
WO (1)	WO1984002351A1 (da)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DK55685A (da) *	1985-02-07	1986-08-08	Nordisk Gentofte	Enzym eller enzymkompleks med proteolytisk aktivitet
GB8412517D0 (en) *	1984-05-16	1984-06-20	Nagai K	Recombinant fusion proteins
US5763215A (en) *	1984-08-16	1998-06-09	Bio-Technology General Corporation	Method of removing N-terminal amino acid residues from eucaryotic polypeptide analogs and polypeptides produced thereby
WO1986001229A1 (en) *	1984-08-16	1986-02-27	Bio-Technology General Corp.	Method of removing n-terminal amino acid residues from eucaryotic polypetide analogs and polypeptides produced thereby
WO1986007380A1 (fr) *	1985-06-04	1986-12-18	Takeda Chemical Industries, Ltd.	Procede de preparation de polypeptides
US4933288A (en) *	1986-11-21	1990-06-12	Cetus Corporation	Use of a modified soluble Pseudomonas exotoxin A in immunoconjugates
JPH01181796A (ja) *	1988-01-14	1989-07-19	Ajinomoto Co Inc	組換えｄｎａ法を用いたポリペプチドの製造法
DE3811921A1 (de) *	1988-04-09	1989-10-19	Hoechst Ag	Verfahren zur herstellung von proteinen, die n-terminal mit prolin beginnen
DE4039415A1 (de)	1990-02-03	1991-08-08	Boehringer Mannheim Gmbh	Verfahren zur herstellung rekombinanter proteine ohne n-terminalen methioninrest
JP2000515017A (ja) *	1996-07-19	2000-11-14	モンサントカンパニー	バクテリア発現系におけるｆ―Ｍｅｔペプチドの脱ホルミル化
US6743600B1 (en)	1999-04-30	2004-06-01	Monsanto Technologies Llc	Method of removing N-terminal alanine residues from polypeptides with Aeromonas aminopeptidase
US6794159B1 (en)	1999-04-30	2004-09-21	Pharmacia Corporation	Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL60184A (en) *	1979-05-31	1984-05-31	Schering Ag	Process for the specific cleavage of protein sequences from proteins
US4532207A (en) *	1982-03-19	1985-07-30	G. D. Searle & Co.	Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase

1983
- 1983-12-09 AU AU23317/84A patent/AU2331784A/en not_active Abandoned
- 1983-12-09 WO PCT/DK1983/000118 patent/WO1984002351A1/en not_active Ceased
- 1983-12-09 JP JP59500221A patent/JPS60500043A/ja active Pending
- 1983-12-09 EP EP84900036A patent/EP0127658B1/en not_active Expired
- 1983-12-09 AT AT84900036T patent/ATE30246T1/de not_active IP Right Cessation
- 1983-12-09 DE DE8484900036T patent/DE3374070D1/de not_active Expired
1984
- 1984-07-13 DK DK344884A patent/DK344884A/da not_active Application Discontinuation

Also Published As

Publication number	Publication date
ATE30246T1 (de)	1987-10-15
EP0127658B1 (en)	1987-10-14
DK344884D0 (da)	1984-07-13
EP0127658A1 (en)	1984-12-12
AU2331784A (en)	1984-07-05
WO1984002351A1 (en)	1984-06-21
DE3374070D1 (en)	1987-11-19
JPS60500043A (ja)	1985-01-17

Legal Events

Date	Code	Title	Description
1988-05-09	ATS	Application withdrawn

Publication	Publication Date	Title
DK1942189T3 (da)	2010-06-07	Fremgangsmåde til fremstilling af sekreterede proteiner
DK344884D0 (da)	1984-07-13	Fremgangsmaade til fremstilling af modne proteiner ud fra fusionsproteiner, syntetiseret i pro- eller eukaryote celler
EP0799317B8 (en)	2006-06-07	Method for secreting thrombopoietin polypeptides
NZ510385A (en)	2002-12-20	E. coli enterotoxin II signal peptide and a microorganism expressing a fusion protein of the MST peptide and a heterologous protein, such as human growth hormone
GB8314362D0 (en)	1983-06-29	Polypeptide and protein products
AU6286986A (en)	1987-03-10	Peptide analogues of mammalian insulin-like growth factor-1
EP0055945A3 (en)	1982-09-15	Human proinsulin and analogs thereof and method of preparation by microbial polypeptide expression and conversion thereof to human insulin
AR004668A1 (es)	1999-03-10	Un acido nucleico aislado purificado, un vector de expresion recombinante que lo comprende, una celula que comprende dicho vector, un metodo para laproduccion de un polipeptido de h.pylori, una composicion de vacuna, el uso de dicha composicion para preparar medicamentos, un polipeptido purificado de h.
ES2000278A6 (es)	1988-02-01	Procedimiento para preparar una proteina de fusion y para preparar una proteina eucariotica.
EP0111389A3 (en)	1985-08-14	Substantially pure porcine growth hormone, dna sequences therefor, and expression vehicles and transfected microorganisms for the production of porcine growth hormone
ES2007425A6 (es)	1989-06-16	Un metodo para la produccion de una proteina con actividad inhibidora del crecimiento de tumores.
JPS6410999A (en)	1989-01-13	Production of physiologically active peptide containing cysteine residue
DE69739013D1 (de)	2008-11-13	Verfahren zur Herstellung sekretorischer KEX2-Derivate
CA2298412A1 (en)	1999-02-04	Identification of human cell lines for the production of human proteins by endogenous gene activation
DE3879823D1 (de)	1993-05-06	Verfahren zur herstellung von menschlichem wachstumshormon.
CA2298106A1 (en)	1999-02-04	A recombinant expression vector of human parathyroid hormone
DE3686365D1 (de)	1992-09-17	Herstellungsverfahren fuer humanes atriales natriuretisches polypeptid.
ES2088911T3 (es)	1996-10-01	Gen de la proteasa i de achromobacter y el producto de este gen.
DK383984A (da)	1985-02-11	Fremgangsmaade til fremstilling af sekretin
ES2061524T3 (es)	1994-12-16	Un vector plasmidico para la expresion en bacillus y empleado para la clonacion del gen estructural que codifica la hormona de crecimiento humana y un metodo para la produccion de la hormona.
ATE42203T1 (de)	1989-05-15	Semi-synthetisches verfahren zur herstellung von insulin.
MX9308031A (es)	1994-08-31	Procedimiento para la produccion de polipeptidos.
SE9701454D0 (sv)	1997-04-18	new DNA sequences and a process for enzyme manufacture
ES2059340T3 (es)	1994-11-16	Un metodo de preparacion de proteinas.
PE20001068A1 (es)	2000-10-18	Produccion de analogos de tripsinogeno recombinante solubles