DK3455372T3 - Polynukleotidberigelse og -amplifikation ved anvendelse af argonautsystemer - Google Patents
Polynukleotidberigelse og -amplifikation ved anvendelse af argonautsystemer Download PDFInfo
- Publication number
- DK3455372T3 DK3455372T3 DK17725052.9T DK17725052T DK3455372T3 DK 3455372 T3 DK3455372 T3 DK 3455372T3 DK 17725052 T DK17725052 T DK 17725052T DK 3455372 T3 DK3455372 T3 DK 3455372T3
- Authority
- DK
- Denmark
- Prior art keywords
- argonauts
- amplification
- enrichment
- polynucleotide
- polynucleotide enrichment
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662335012P | 2016-05-11 | 2016-05-11 | |
| PCT/US2017/032021 WO2017197027A1 (en) | 2016-05-11 | 2017-05-10 | Polynucleotide enrichment and amplification using argonaute systems |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3455372T3 true DK3455372T3 (da) | 2020-01-06 |
Family
ID=58745435
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17725052.9T DK3455372T3 (da) | 2016-05-11 | 2017-05-10 | Polynukleotidberigelse og -amplifikation ved anvendelse af argonautsystemer |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US11542544B2 (da) |
| EP (3) | EP3656873A3 (da) |
| CN (2) | CN116397007A (da) |
| DK (1) | DK3455372T3 (da) |
| WO (1) | WO2017197027A1 (da) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3246416B1 (en) | 2011-04-15 | 2024-06-05 | The Johns Hopkins University | Safe sequencing system |
| WO2014070462A1 (en) | 2012-10-29 | 2014-05-08 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
| US11286531B2 (en) | 2015-08-11 | 2022-03-29 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| EP4491740B1 (en) * | 2016-09-02 | 2026-04-15 | New England Biolabs, Inc. | Analysis of chromatin using a nicking enzyme |
| KR20250047324A (ko) | 2017-08-07 | 2025-04-03 | 더 존스 홉킨스 유니버시티 | 암을 평가하고 치료하기 위한 방법 및 재료 |
| CN109055499B (zh) * | 2018-08-30 | 2021-01-19 | 杭州杰毅生物技术有限公司 | 基于CRISPR-Cas的等温核酸检测方法及试剂盒 |
| CN113166798B (zh) * | 2018-11-28 | 2025-05-02 | 主基因有限公司 | 通过核酸内切酶保护的靶向富集 |
| CN109880891B (zh) * | 2019-04-22 | 2021-07-30 | 上海交通大学 | 基于核酸酶偶联pcr原理富集低丰度dna突变的检测技术体系及应用 |
| JP2023519782A (ja) * | 2020-01-17 | 2023-05-15 | ジャンプコード ゲノミクス,インク. | 標的化された配列決定の方法 |
| WO2021163546A1 (en) | 2020-02-14 | 2021-08-19 | The Johns Hopkins University | Methods and materials for assessing nucleic acids |
| EP4118203A4 (en) * | 2020-03-11 | 2024-03-27 | The Broad Institute, Inc. | NEW ENZYMES CASES AND METHODS FOR SPECIFICITY AND ACTIVITY PROFILING |
| CN113201586B (zh) * | 2021-04-22 | 2024-03-26 | 苏州淦江生物技术有限公司 | 一种基于Cas蛋白的检测方法 |
| CN114250276B (zh) * | 2021-12-13 | 2024-04-30 | 复旦大学 | 基于指数扩增反应和Argonaute核酸酶的microRNA检测体系及方法 |
| CN115927539A (zh) * | 2022-09-14 | 2023-04-07 | 首都体育学院 | 靶标核酸富集方法及其试剂盒和应用 |
| CA3268411A1 (en) * | 2022-09-23 | 2024-03-28 | Flagship Pioneering Innovations Vi, Llc | METHODS FOR ENRICHING TARGET NUCLEIC ACID SEQUENCES |
| CN117660609B (zh) * | 2023-11-24 | 2024-07-23 | 湖北大学 | 基于LwaCas13a的非扩增核酸检测组合物、试剂盒和方法 |
| CN119286625B (zh) * | 2024-08-13 | 2025-08-22 | 南京市第一医院 | 一种基于等温扩增与CRSPR/Cas12a技术的B族链球菌即时检测装置 |
Family Cites Families (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
| US6261808B1 (en) | 1992-08-04 | 2001-07-17 | Replicon, Inc. | Amplification of nucleic acid molecules via circular replicons |
| US5834202A (en) | 1992-08-04 | 1998-11-10 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| WO1994003624A1 (en) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US5614389A (en) | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5593826A (en) | 1993-03-22 | 1997-01-14 | Perkin-Elmer Corporation, Applied Biosystems, Inc. | Enzymatic ligation of 3'amino-substituted oligonucleotides |
| ES2204913T3 (es) | 1993-04-12 | 2004-05-01 | Northwestern University | Metodo para formacion de oligonucleotidos. |
| US5422252A (en) | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
| CA2122203C (en) | 1993-05-11 | 2001-12-18 | Melinda S. Fraiser | Decontamination of nucleic acid amplification reactions |
| JP3026843B2 (ja) | 1993-07-23 | 2000-03-27 | ジェン−プローブ・インコーポレーテッド | 核酸増幅の促進法 |
| FR2708288B1 (fr) | 1993-07-26 | 1995-09-01 | Bio Merieux | Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé. |
| US5523204A (en) | 1993-12-10 | 1996-06-04 | Becton Dickinson And Company | Detection of nucleic acids in cells by strand displacement amplification |
| SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| CA2185239C (en) | 1994-03-16 | 2002-12-17 | Nanibhushan Dattagupta | Isothermal strand displacement nucleic acid amplification |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| FR2726277B1 (fr) | 1994-10-28 | 1996-12-27 | Bio Merieux | Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin |
| US5631147A (en) | 1995-09-21 | 1997-05-20 | Becton, Dickinson And Company | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
| US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
| JP2000505312A (ja) | 1996-03-18 | 2000-05-09 | モレキュラー バイオロジー リソーシーズ,インコーポレイテッド | 標的核酸配列増幅 |
| US5773733A (en) | 1996-04-12 | 1998-06-30 | National Science Council | Alumina-aluminum nitride-nickel composites |
| US20080119427A1 (en) * | 1996-06-06 | 2008-05-22 | Isis Pharmaceuticals, Inc. | Double Strand Compositions Comprising Differentially Modified Strands for Use in Gene Modulation |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| EP2327797B1 (en) | 1997-04-01 | 2015-11-25 | Illumina Cambridge Limited | Method of nucleic acid sequencing |
| US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| EP1020534B2 (en) | 1998-11-09 | 2011-01-05 | Eiken Kagaku Kabushiki Kaisha | Process for synthesizing nucleic acid |
| US6155972A (en) | 1999-02-02 | 2000-12-05 | Acorn Cardiovascular, Inc. | Cardiac constraint jacket construction |
| ATE362532T1 (de) | 1999-03-19 | 2007-06-15 | Takara Bio Inc | Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers |
| US6326173B1 (en) | 1999-04-12 | 2001-12-04 | Nanogen/Becton Dickinson Partnership | Electronically mediated nucleic acid amplification in NASBA |
| US6309833B1 (en) | 1999-04-12 | 2001-10-30 | Nanogen/Becton Dickinson Partnership | Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures |
| US6238868B1 (en) | 1999-04-12 | 2001-05-29 | Nanogen/Becton Dickinson Partnership | Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology |
| US20050244870A1 (en) | 1999-04-20 | 2005-11-03 | Illumina, Inc. | Nucleic acid sequencing using microsphere arrays |
| ATE262595T1 (de) | 1999-09-13 | 2004-04-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| EP2100971A3 (en) | 2000-07-07 | 2009-11-25 | Visigen Biotechnologies, Inc. | Real-time sequence determination |
| AU7878301A (en) | 2000-08-23 | 2002-03-04 | Takara Shuzo Co | Method of amplifying nucleic acid |
| US7211414B2 (en) | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| AU2003259350A1 (en) | 2002-08-23 | 2004-03-11 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| US7595883B1 (en) | 2002-09-16 | 2009-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Biological analysis arrangement and approach therefor |
| AU2003297557B2 (en) | 2002-11-21 | 2009-02-26 | Cellscript, Inc. | Methods for using primers that encode one strand of a double-stranded promoter |
| US20050153333A1 (en) | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
| US7315019B2 (en) | 2004-09-17 | 2008-01-01 | Pacific Biosciences Of California, Inc. | Arrays of optical confinements and uses thereof |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| SG170802A1 (en) | 2006-03-31 | 2011-05-30 | Solexa Inc | Systems and devices for sequence by synthesis analysis |
| EP2089517A4 (en) | 2006-10-23 | 2010-10-20 | Pacific Biosciences California | POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| EP2677309B9 (en) | 2006-12-14 | 2014-11-19 | Life Technologies Corporation | Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| EP2664678B1 (en) | 2008-10-24 | 2014-10-08 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| JP6017458B2 (ja) * | 2011-02-02 | 2016-11-02 | ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション | 大量並列連続性マッピング |
| GB201113214D0 (en) * | 2011-07-29 | 2011-09-14 | Univ East Anglia | Analysing sequencing bias |
| ES2749624T3 (es) * | 2013-03-14 | 2020-03-23 | Caribou Biosciences Inc | Composiciones y métodos de ácidos nucleicos dirigidos a ácido nucleico |
| US9328382B2 (en) * | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
| US9902973B2 (en) * | 2013-04-11 | 2018-02-27 | Caribou Biosciences, Inc. | Methods of modifying a target nucleic acid with an argonaute |
| US9850525B2 (en) * | 2014-01-29 | 2017-12-26 | Agilent Technologies, Inc. | CAS9-based isothermal method of detection of specific DNA sequence |
| JP6416939B2 (ja) * | 2014-02-13 | 2018-10-31 | タカラ バイオ ユーエスエー,インコーポレイティド | 核酸の初期収集物から標的分子を減損させる方法、並びにそれを実施するための組成物及びキット |
| EP3119897A1 (en) * | 2014-03-21 | 2017-01-25 | Cellectis | Engineering mammalian genome using dna-guided argonaute interference systems (dais) |
| EP3172321B2 (en) * | 2014-07-21 | 2023-01-04 | Illumina, Inc. | Polynucleotide enrichment using crispr-cas systems |
| SG11201703779VA (en) | 2014-11-11 | 2017-06-29 | Illumina Inc | Polynucleotide amplification using crispr-cas systems |
-
2017
- 2017-05-10 EP EP19205523.4A patent/EP3656873A3/en not_active Withdrawn
- 2017-05-10 WO PCT/US2017/032021 patent/WO2017197027A1/en not_active Ceased
- 2017-05-10 CN CN202310557305.1A patent/CN116397007A/zh active Pending
- 2017-05-10 US US16/099,953 patent/US11542544B2/en active Active
- 2017-05-10 EP EP23188809.0A patent/EP4269611A3/en not_active Withdrawn
- 2017-05-10 DK DK17725052.9T patent/DK3455372T3/da active
- 2017-05-10 CN CN201780044607.1A patent/CN109477137B/zh not_active Expired - Fee Related
- 2017-05-10 EP EP17725052.9A patent/EP3455372B1/en active Active
-
2022
- 2022-12-09 US US18/063,865 patent/US12391983B2/en active Active
-
2025
- 2025-07-29 US US19/283,715 patent/US20260117291A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3656873A3 (en) | 2020-07-29 |
| US20230295701A1 (en) | 2023-09-21 |
| EP3455372A1 (en) | 2019-03-20 |
| US12391983B2 (en) | 2025-08-19 |
| US11542544B2 (en) | 2023-01-03 |
| US20260117291A1 (en) | 2026-04-30 |
| CN109477137B (zh) | 2023-05-30 |
| EP4269611A2 (en) | 2023-11-01 |
| CN109477137A (zh) | 2019-03-15 |
| CN116397007A (zh) | 2023-07-07 |
| EP3656873A2 (en) | 2020-05-27 |
| EP4269611A3 (en) | 2024-01-17 |
| US20190136303A1 (en) | 2019-05-09 |
| WO2017197027A1 (en) | 2017-11-16 |
| EP3455372B1 (en) | 2019-10-30 |
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