DK3577219T3 - Alfa-amylase-varianter - Google Patents

Alfa-amylase-varianter Download PDF

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Publication number
DK3577219T3
DK3577219T3 DK18708589.9T DK18708589T DK3577219T3 DK 3577219 T3 DK3577219 T3 DK 3577219T3 DK 18708589 T DK18708589 T DK 18708589T DK 3577219 T3 DK3577219 T3 DK 3577219T3
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DK
Denmark
Prior art keywords
alpha
amylase variants
amylase
variants
Prior art date
Application number
DK18708589.9T
Other languages
English (en)
Other versions
DK3577219T5 (da
Inventor
Carsten Andersen
Iben Damager
Chakshusmathi Ghadiyaram
Rajendra Kulothungan Sainathan
Padma Venkatachalam Iyer
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK3577219T3 publication Critical patent/DK3577219T3/da
Publication of DK3577219T5 publication Critical patent/DK3577219T5/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618Protease or amylase in liquid compositions only
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001Alpha-amylase (3.2.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
DK18708589.9T 2017-02-01 2018-01-30 Alfa-amylase-varianter DK3577219T5 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN201741003686 2017-02-01
EP17163114 2017-03-27
PCT/EP2018/052197 WO2018141707A2 (en) 2017-02-01 2018-01-30 Alpha-amylase variants

Publications (2)

Publication Number Publication Date
DK3577219T3 true DK3577219T3 (da) 2023-11-13
DK3577219T5 DK3577219T5 (da) 2024-09-02

Family

ID=61563337

Family Applications (1)

Application Number Title Priority Date Filing Date
DK18708589.9T DK3577219T5 (da) 2017-02-01 2018-01-30 Alfa-amylase-varianter

Country Status (11)

Country Link
US (2) US12071643B2 (da)
EP (2) EP4276179A3 (da)
JP (3) JP7242535B2 (da)
CN (2) CN118879666A (da)
BR (1) BR112019015943A2 (da)
CA (1) CA3046670A1 (da)
DK (1) DK3577219T5 (da)
ES (1) ES2963486T3 (da)
MX (2) MX2019008967A (da)
WO (1) WO2018141707A2 (da)
ZA (1) ZA201905673B (da)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3051426C (en) * 2017-02-01 2023-10-17 The Procter & Gamble Company Cleaning compositions comprising amylase variants
WO2020114965A1 (en) 2018-12-03 2020-06-11 Novozymes A/S LOW pH POWDER DETERGENT COMPOSITION
WO2020114968A1 (en) 2018-12-03 2020-06-11 Novozymes A/S Powder detergent compositions
EP3702452A1 (en) 2019-03-01 2020-09-02 Novozymes A/S Detergent compositions comprising two proteases
WO2020264552A1 (en) * 2019-06-24 2020-12-30 The Procter & Gamble Company Cleaning compositions comprising amylase variants
CN113993996A (zh) * 2019-06-24 2022-01-28 诺维信公司 α-淀粉酶变体
JP7824218B2 (ja) * 2019-12-19 2026-03-04 ノボザイムス アクティーゼルスカブ α-アミラーゼ変異体
CA3160401C (en) * 2019-12-19 2025-06-10 The Procter & Gamble Company CLEANING COMPOSITIONS COMPRISING POLYPEPTIDES HAVING ALPHA-AMYLASE ACTIVITY
US20230212548A1 (en) 2020-05-26 2023-07-06 Novozymes A/S Subtilase variants and compositions comprising same
WO2022043563A1 (en) 2020-08-28 2022-03-03 Novozymes A/S Polyester degrading protease variants
JP7772526B2 (ja) * 2020-10-02 2025-11-18 花王株式会社 α-アミラーゼ変異体
WO2022071451A1 (ja) * 2020-10-02 2022-04-07 花王株式会社 α-アミラーゼ変異体
EP4047088A1 (en) * 2021-02-22 2022-08-24 Basf Se Amylase variants
JP2023095355A (ja) * 2021-12-24 2023-07-06 花王株式会社 アミラーゼ配合洗浄剤組成物

Family Cites Families (24)

* Cited by examiner, † Cited by third party
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DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
JP3649338B2 (ja) 1994-06-03 2005-05-18 ノボザイムス バイオテック,インコーポレイティド 精製されたマイセリオフトラ・ラッカーゼ及びそれをコードする核酸
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
EG21623A (en) 1996-04-16 2001-12-31 Procter & Gamble Mid-chain branced surfactants
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CN1940067A (zh) 1999-03-22 2007-04-04 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
ES2532606T3 (es) 1999-03-31 2015-03-30 Novozymes A/S Polipéptidos con actividad de alfa-amilasa alcalina y ácidos nucleicos que los codifican
EP1214442A1 (en) 1999-09-01 2002-06-19 Novozymes A/S Method for production of maltose and/or enzymatically modified starch
CA2702204C (en) 2000-08-01 2011-09-06 Novozymes A/S Alpha-amylase mutants with altered properties
WO2002042740A1 (en) 2000-11-27 2002-05-30 Novozymes A/S Automated mechanical stress assay for screening cleaning ingredients
US7314712B2 (en) 2001-07-27 2008-01-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
MX369096B (es) * 2010-02-10 2019-10-29 Novozymes As Variantes y composiciones que comprenden variantes con alta estabilidad en presencia de un agente quelante.
ES2834102T3 (es) * 2011-06-30 2021-06-16 Novozymes As Variantes de alfa-amilasa
EP2540824A1 (en) * 2011-06-30 2013-01-02 The Procter & Gamble Company Cleaning compositions comprising amylase variants reference to a sequence listing
US20150353871A1 (en) * 2012-11-30 2015-12-10 Novozymes A/S Polypeptides for Cleaning or Detergent Compositions
US20160348084A1 (en) * 2013-09-30 2016-12-01 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
EP3083952A2 (en) 2013-12-20 2016-10-26 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
CN106414729A (zh) * 2014-06-12 2017-02-15 诺维信公司 α‑淀粉酶变体以及对其进行编码的多核苷酸
JP7175612B2 (ja) * 2015-05-08 2022-11-21 ノボザイムス アクティーゼルスカブ アルファ-アミラーゼ多様体およびこの多様体をコードするポリヌクレオチド

Also Published As

Publication number Publication date
CA3046670A1 (en) 2018-08-09
WO2018141707A2 (en) 2018-08-09
ES2963486T3 (es) 2024-03-27
US20240409909A1 (en) 2024-12-12
JP2020506698A (ja) 2020-03-05
US12071643B2 (en) 2024-08-27
EP3577219B1 (en) 2023-08-23
MX2023008937A (es) 2023-08-11
WO2018141707A3 (en) 2019-02-07
DK3577219T5 (da) 2024-09-02
BR112019015943A2 (pt) 2020-04-07
MX2019008967A (es) 2019-09-10
JP7712971B2 (ja) 2025-07-24
EP4276179A3 (en) 2024-05-22
JP2023071943A (ja) 2023-05-23
ZA201905673B (en) 2024-06-26
JP7242535B2 (ja) 2023-03-20
CN110382694A (zh) 2019-10-25
EP4276179A2 (en) 2023-11-15
JP2025143481A (ja) 2025-10-01
CN118879666A (zh) 2024-11-01
US20190345470A1 (en) 2019-11-14
EP3577219A2 (en) 2019-12-11
CN110382694B (zh) 2024-07-30

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