DK3577219T5 - Alfa-amylase-varianter - Google Patents

Alfa-amylase-varianter Download PDF

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Publication number
DK3577219T5
DK3577219T5 DK18708589.9T DK18708589T DK3577219T5 DK 3577219 T5 DK3577219 T5 DK 3577219T5 DK 18708589 T DK18708589 T DK 18708589T DK 3577219 T5 DK3577219 T5 DK 3577219T5
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DK
Denmark
Prior art keywords
alpha
amylase variants
amylase
variants
Prior art date
Application number
DK18708589.9T
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English (en)
Other versions
DK3577219T3 (da
Inventor
Carsten Andersen
Iben Damager
Chakshusmathi Ghadiyaram
Rajendra Kulothungan Sainathan
Padma Venkatachalam Iyer
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of DK3577219T3 publication Critical patent/DK3577219T3/da
Application granted granted Critical
Publication of DK3577219T5 publication Critical patent/DK3577219T5/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618Protease or amylase in liquid compositions only
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001Alpha-amylase (3.2.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
DK18708589.9T 2017-02-01 2018-01-30 Alfa-amylase-varianter DK3577219T5 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN201741003686 2017-02-01
EP17163114 2017-03-27
PCT/EP2018/052197 WO2018141707A2 (en) 2017-02-01 2018-01-30 Alpha-amylase variants

Publications (2)

Publication Number Publication Date
DK3577219T3 DK3577219T3 (da) 2023-11-13
DK3577219T5 true DK3577219T5 (da) 2024-09-02

Family

ID=61563337

Family Applications (1)

Application Number Title Priority Date Filing Date
DK18708589.9T DK3577219T5 (da) 2017-02-01 2018-01-30 Alfa-amylase-varianter

Country Status (11)

Country Link
US (2) US12071643B2 (da)
EP (2) EP3577219B1 (da)
JP (3) JP7242535B2 (da)
CN (2) CN118879666A (da)
BR (1) BR112019015943A2 (da)
CA (1) CA3046670A1 (da)
DK (1) DK3577219T5 (da)
ES (1) ES2963486T3 (da)
MX (2) MX2019008967A (da)
WO (1) WO2018141707A2 (da)
ZA (1) ZA201905673B (da)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110234747B (zh) 2017-02-01 2021-11-09 宝洁公司 包含淀粉酶变体的清洁组合物
CN113302270A (zh) 2018-12-03 2021-08-24 诺维信公司 低pH粉末洗涤剂组合物
US20220056379A1 (en) 2018-12-03 2022-02-24 Novozymes A/S Powder Detergent Compositions
EP3702452A1 (en) 2019-03-01 2020-09-02 Novozymes A/S Detergent compositions comprising two proteases
MX2021015382A (es) * 2019-06-24 2022-01-24 Procter & Gamble Composiciones de limpieza que comprenden variantes de amilasa.
EP3987022A1 (en) * 2019-06-24 2022-04-27 Novozymes A/S Alpha-amylase variants
CN114829565A (zh) * 2019-12-19 2022-07-29 宝洁公司 包含具有α淀粉酶活性的多肽的清洁组合物
CN114945664A (zh) * 2019-12-19 2022-08-26 诺维信公司 α-淀粉酶变体
WO2021239818A1 (en) 2020-05-26 2021-12-02 Novozymes A/S Subtilase variants and compositions comprising same
US20230265408A1 (en) 2020-08-28 2023-08-24 Novozymes A/S Protease variants with improved solubility
JP7772526B2 (ja) * 2020-10-02 2025-11-18 花王株式会社 α-アミラーゼ変異体
WO2022071451A1 (ja) * 2020-10-02 2022-04-07 花王株式会社 α-アミラーゼ変異体
EP4047088A1 (en) * 2021-02-22 2022-08-24 Basf Se Amylase variants
JP2023095355A (ja) * 2021-12-24 2023-07-06 花王株式会社 アミラーゼ配合洗浄剤組成物

Family Cites Families (24)

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DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
WO1995033836A1 (en) 1994-06-03 1995-12-14 Novo Nordisk Biotech, Inc. Phosphonyldipeptides useful in the treatment of cardiovascular diseases
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
PH11997056158B1 (en) 1996-04-16 2001-10-15 Procter & Gamble Mid-chain branched primary alkyl sulphates as surfactants
EG21623A (en) 1996-04-16 2001-12-31 Procter & Gamble Mid-chain branced surfactants
CN100482801C (zh) 1999-03-22 2009-04-29 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
JP4745503B2 (ja) 1999-03-31 2011-08-10 ノボザイムス アクティーゼルスカブ アルカリα−アミラーゼ活性を有するポリペプチド及びそれらをコードする核酸
AU6686100A (en) 1999-09-01 2001-03-26 Novozymes A/S Method for production of maltose and/or enzymatically modified starch
EP1370648A2 (en) 2000-08-01 2003-12-17 Novozymes A/S Alpha-amylase mutants with altered properties
AU2302002A (en) 2000-11-27 2002-06-03 Novozymes As Automated mechanical stress assay for screening cleaning ingredients
EP1421187B1 (en) 2001-07-27 2007-10-10 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Systems for in vivo site-directed mutagenesis using oligonucleotides
US9896673B2 (en) * 2010-02-10 2018-02-20 Novozymes A/S Compositions of high stability alpha amylase variants
ES2834102T3 (es) * 2011-06-30 2021-06-16 Novozymes As Variantes de alfa-amilasa
EP2540824A1 (en) 2011-06-30 2013-01-02 The Procter & Gamble Company Cleaning compositions comprising amylase variants reference to a sequence listing
EP2925849A2 (en) 2012-11-30 2015-10-07 Novozymes A/S Polypeptides for cleaning or detergent compositions
US20160348084A1 (en) * 2013-09-30 2016-12-01 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US20160312200A1 (en) 2013-12-20 2016-10-27 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US20170121695A1 (en) * 2014-06-12 2017-05-04 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
CA2981409A1 (en) * 2015-05-08 2016-11-17 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same

Also Published As

Publication number Publication date
US20240409909A1 (en) 2024-12-12
CA3046670A1 (en) 2018-08-09
MX2023008937A (es) 2023-08-11
JP7712971B2 (ja) 2025-07-24
CN118879666A (zh) 2024-11-01
WO2018141707A2 (en) 2018-08-09
CN110382694A (zh) 2019-10-25
JP2020506698A (ja) 2020-03-05
MX2019008967A (es) 2019-09-10
BR112019015943A2 (pt) 2020-04-07
DK3577219T3 (da) 2023-11-13
CN110382694B (zh) 2024-07-30
EP4276179A2 (en) 2023-11-15
JP2023071943A (ja) 2023-05-23
WO2018141707A3 (en) 2019-02-07
EP3577219A2 (en) 2019-12-11
EP3577219B1 (en) 2023-08-23
ZA201905673B (en) 2024-06-26
JP2025143481A (ja) 2025-10-01
US20190345470A1 (en) 2019-11-14
JP7242535B2 (ja) 2023-03-20
US12071643B2 (en) 2024-08-27
ES2963486T3 (es) 2024-03-27
EP4276179A3 (en) 2024-05-22

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