DK3899027T3 - Identifikation af beslægtede par af ligander og en receptorer - Google Patents

Identifikation af beslægtede par af ligander og en receptorer Download PDF

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Publication number
DK3899027T3
DK3899027T3 DK19828270.9T DK19828270T DK3899027T3 DK 3899027 T3 DK3899027 T3 DK 3899027T3 DK 19828270 T DK19828270 T DK 19828270T DK 3899027 T3 DK3899027 T3 DK 3899027T3
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DK
Denmark
Prior art keywords
ligands
receptors
identification
related pairs
pairs
Prior art date
Application number
DK19828270.9T
Other languages
English (en)
Inventor
Andrew Griffiths
Sebastien Amigorena
Olivier Lantz
David Weitz
Philippe Nghe
Annabelle Gerard
Original Assignee
Paris Sciences Et Lettres
Centre Nat Rech Scient
Inst Curie
The President And Fellows Of Harvard College
Hifibio Hk Ltd
Inst Nat Sante Rech Med
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Application filed by Paris Sciences Et Lettres, Centre Nat Rech Scient, Inst Curie, The President And Fellows Of Harvard College, Hifibio Hk Ltd, Inst Nat Sante Rech Med filed Critical Paris Sciences Et Lettres
Application granted granted Critical
Publication of DK3899027T3 publication Critical patent/DK3899027T3/da

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/0005Vertebrate antigens
    • A61K39/0011Cancer antigens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/149Modifications characterised by incorporating a coding sequence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122Massive parallel sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/629Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Oncology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK19828270.9T 2018-12-19 2019-12-19 Identifikation af beslægtede par af ligander og en receptorer DK3899027T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18306741.2A EP3670667A1 (en) 2018-12-19 2018-12-19 Identification of cognate pairs of ligands and receptors
PCT/EP2019/086334 WO2020127754A1 (en) 2018-12-19 2019-12-19 Identification of cognate pairs of ligands and receptors

Publications (1)

Publication Number Publication Date
DK3899027T3 true DK3899027T3 (da) 2023-05-01

Family

ID=65324139

Family Applications (3)

Application Number Title Priority Date Filing Date
DK23163171.4T DK4234712T3 (da) 2018-12-19 2019-12-19 Identifikation af kognitive par af ligander og receptorer
DK24195348.8T DK4495236T3 (da) 2018-12-19 2019-12-19 Identifikation af beslægtede par af ligander og receptorer
DK19828270.9T DK3899027T3 (da) 2018-12-19 2019-12-19 Identifikation af beslægtede par af ligander og en receptorer

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DK23163171.4T DK4234712T3 (da) 2018-12-19 2019-12-19 Identifikation af kognitive par af ligander og receptorer
DK24195348.8T DK4495236T3 (da) 2018-12-19 2019-12-19 Identifikation af beslægtede par af ligander og receptorer

Country Status (5)

Country Link
US (1) US20220065849A1 (da)
EP (4) EP3670667A1 (da)
DK (3) DK4234712T3 (da)
LT (3) LT4495236T (da)
WO (1) WO2020127754A1 (da)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3215070A1 (en) * 2021-04-14 2022-10-20 Joseph DE RUTTE Nanoscale reaction chambers and methods of using the same
EP4490737A1 (en) * 2022-03-08 2025-01-15 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
EP4592676A1 (en) * 2024-01-29 2025-07-30 Saber Bio SAS Methods and systems for screening ligands and receptors

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
EP2127736A1 (en) 2003-04-10 2009-12-02 The President and Fellows of Harvard College Formation and control of fluidic species
JP2006349060A (ja) 2005-06-16 2006-12-28 Ntn Corp ボールねじ
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2008130977A2 (en) 2007-04-16 2008-10-30 The General Hospital Corporation D/B/A Massachusetts General Hospital Systems and methods for particle focusing in microchannels
US9068181B2 (en) 2008-05-23 2015-06-30 The General Hospital Corporation Microfluidic droplet encapsulation
US20110223314A1 (en) 2010-03-10 2011-09-15 Xiaoxiao Zhang Efficient microencapsulation
DK2823060T3 (da) * 2012-03-05 2018-05-28 Adaptive Biotechnologies Corp Bestemmelse af forbundne immunreceptorkæder fra hyppighedsafstemte underenheder
AU2014245806B2 (en) * 2013-03-28 2019-10-31 The University Of British Columbia Microfluidic devices and methods for use thereof in multicellular assays of secretion
WO2017013170A1 (en) * 2015-07-22 2017-01-26 F. Hoffmann-La Roche Ag Identification of antigen epitopes and immune sequences recognizing the antigens
MA44007A (fr) * 2016-02-05 2018-12-19 Denali Therapeutics Inc Inhibiteurs du récepteur interagissant avec protéine kinase 1
TWI758298B (zh) * 2016-06-23 2022-03-21 國立研究開發法人理化學研究所 利用單步驟逆轉錄模板置換pcr之t細胞受體及b細胞受體多樣性解析系統
JP6916309B2 (ja) * 2017-03-13 2021-08-11 ギガジェン,インコーポレイティッド 単一細胞の大規模並行コンビナトリアル分析のためのシステム及び方法

Also Published As

Publication number Publication date
EP4234712A3 (en) 2023-09-27
EP3899027B1 (en) 2023-03-22
EP4234712B1 (en) 2024-08-21
EP3670667A1 (en) 2020-06-24
LT4234712T (lt) 2025-01-10
EP3899027A1 (en) 2021-10-27
EP4495236A3 (en) 2025-02-26
DK4495236T3 (da) 2026-03-02
EP4495236A2 (en) 2025-01-22
US20220065849A1 (en) 2022-03-03
EP4234712A2 (en) 2023-08-30
LT4495236T (lt) 2026-04-10
EP4495236B1 (en) 2026-02-11
WO2020127754A1 (en) 2020-06-25
DK4234712T3 (da) 2024-10-28
LT3899027T (lt) 2023-07-10

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