DK4110957T3 - Fremgangsmåder til analyse af cellefrie nukleinsyrer og anvendelser heraf - Google Patents

Fremgangsmåder til analyse af cellefrie nukleinsyrer og anvendelser heraf

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Publication number
DK4110957T3
DK4110957T3 DK21759938.0T DK21759938T DK4110957T3 DK 4110957 T3 DK4110957 T3 DK 4110957T3 DK 21759938 T DK21759938 T DK 21759938T DK 4110957 T3 DK4110957 T3 DK 4110957T3
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Denmark
Prior art keywords
analysis
applications
cell
methods
nucleic acids
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Application number
DK21759938.0T
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English (en)
Inventor
Maximilian Diehn
Arash Ash Alizadeh
Jacob J Chabon
David M Kurtz
Mohammad Shahrokh Esfahani
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Univ Leland Stanford Junior
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Publication of DK4110957T3 publication Critical patent/DK4110957T3/da

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1082Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/20Supervised data analysis
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16HHEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
    • G16H50/00ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
    • G16H50/20ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16HHEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
    • G16H50/00ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
    • G16H50/30ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for calculating health indices; for individual health risk assessment
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Public Health (AREA)
  • Data Mining & Analysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Databases & Information Systems (AREA)
  • Evolutionary Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Software Systems (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Evolutionary Computation (AREA)
  • Bioethics (AREA)
  • Artificial Intelligence (AREA)
  • Primary Health Care (AREA)
DK21759938.0T 2020-02-24 2021-02-24 Fremgangsmåder til analyse af cellefrie nukleinsyrer og anvendelser heraf DK4110957T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202062980972P 2020-02-24 2020-02-24
PCT/US2021/019478 WO2021173722A2 (en) 2020-02-24 2021-02-24 Methods of analyzing cell free nucleic acids and applications thereof

Publications (1)

Publication Number Publication Date
DK4110957T3 true DK4110957T3 (da) 2025-10-27

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Family Applications (1)

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DK21759938.0T DK4110957T3 (da) 2020-02-24 2021-02-24 Fremgangsmåder til analyse af cellefrie nukleinsyrer og anvendelser heraf

Country Status (9)

Country Link
US (2) US20230242980A1 (da)
EP (3) EP4660324A3 (da)
KR (2) KR20220157976A (da)
CN (2) CN116113436A (da)
AU (2) AU2021227229A1 (da)
CA (2) CA3172670A1 (da)
DK (1) DK4110957T3 (da)
ES (1) ES3040741T3 (da)
WO (2) WO2021173724A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11728007B2 (en) * 2017-11-30 2023-08-15 Grail, Llc Methods and systems for analyzing nucleic acid sequences using mappability analysis and de novo sequence assembly
GB2623904B (en) 2019-11-06 2024-07-24 Univ Leland Stanford Junior Methods and systems for analyzing nucleic acid molecules
US11783912B2 (en) 2021-05-05 2023-10-10 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for analyzing nucleic acid molecules
MX2024003132A (es) * 2021-09-13 2024-04-10 Regeneron Pharma Metodos para tratar la hematopoyesis clonal de potencial indeterminado (chip) con inhibidores del antigeno linfocitario 75 (ly75), grupo de diferenciacion 164 (cd164) o poli (adp-ribosa) polimerasa 1 (parp1).
EP4433611A4 (en) * 2021-11-17 2025-10-08 Univ Leland Stanford Junior SYSTEMS AND METHODS FOR GENE EXPRESSION AND TISSUE OF ORIGIN INFERENCE FROM CIRCULATING FREE DNA
JP2025536559A (ja) * 2022-11-01 2025-11-07 ゾエティス・サービシーズ・エルエルシー 非がん性体細胞変異の検出
WO2025096466A2 (en) * 2023-10-30 2025-05-08 Myriad Women's Health, Inc. Methods for improving minimal residual disease assays
WO2025212823A1 (en) * 2024-04-03 2025-10-09 Memorial Sloan-Kettering Cancer Center Methods for predicting risk of recurrence and metastases in lung cancer patients using circulating tumor dna and spatial biomarkers

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA186569A (en) 1918-04-02 1918-09-17 Henry Arthur Dygert Surgical appliance
CA188298A (en) 1918-04-08 1919-01-14 William H. Bot Grain separator
US6171856B1 (en) * 1997-07-30 2001-01-09 Board Of Regents, The University Of Texas System Methods and compositions relating to no-mediated cytotoxicity
KR20030007463A (ko) * 2000-03-16 2003-01-23 이콜로지컬 테크놀로지 리서치, 엘티디. Dna 손상 지표를 이용한 천연 및 인공 화학물질의 간이생물학적 평가법, 및 그 장치
AU2001255518A1 (en) * 2000-06-07 2001-12-17 Baylor College Of Medicine Compositions and methods for array-based nucleic acid hybridization
DE10032165A1 (de) * 2000-07-01 2002-01-10 Beiersdorf Ag Verwendung von physiologisch verträglichen Sulfinsäuren als Antioxidans oder Radikalfänger in kosmetischen oder dermatologischen Zubereitungen
EP3290530B1 (en) * 2009-02-18 2020-09-02 Streck Inc. Preservation of cell-free nucleic acids
ES2925014T3 (es) * 2014-09-12 2022-10-13 Univ Leland Stanford Junior Identificación y uso de ácidos nucleicos circulantes
WO2018081161A1 (en) * 2016-10-24 2018-05-03 Biomatrica, Inc. Stabilization of nucleic acids on paper
US11479807B2 (en) * 2017-03-23 2022-10-25 University Of Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
US11584958B2 (en) * 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
US11542540B2 (en) * 2017-06-16 2023-01-03 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers

Also Published As

Publication number Publication date
CN115443341A (zh) 2022-12-06
AU2021227229A1 (en) 2022-10-13
CA3172670A1 (en) 2021-09-02
EP4110957B1 (en) 2025-08-27
US20250263789A1 (en) 2025-08-21
WO2021173722A2 (en) 2021-09-02
US20230242980A1 (en) 2023-08-03
ES3040741T3 (en) 2025-11-04
WO2021173724A1 (en) 2021-09-02
EP4660324A2 (en) 2025-12-10
EP4110957A4 (en) 2024-03-06
AU2021225854A1 (en) 2022-10-13
EP4110397A1 (en) 2023-01-04
EP4660324A3 (en) 2026-02-18
EP4110957A2 (en) 2023-01-04
EP4110397A4 (en) 2024-05-01
CA3172675A1 (en) 2021-09-02
KR20220157976A (ko) 2022-11-29
KR20220145891A (ko) 2022-10-31
CN116113436A (zh) 2023-05-12
WO2021173722A3 (en) 2021-10-07

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