DK504585A - Rekombinante plasmider, bakterier med indhold heraf, fremgangsmaade til fremstilling af fermenterede foedevareprodukter, dyrefoder, bestanddele hertil eller proteiner ved hjaelp af saadanne bakterier - Google Patents

Rekombinante plasmider, bakterier med indhold heraf, fremgangsmaade til fremstilling af fermenterede foedevareprodukter, dyrefoder, bestanddele hertil eller proteiner ved hjaelp af saadanne bakterier Download PDF

Info

Publication number
DK504585A
DK504585A DK504585A DK504585A DK504585A DK 504585 A DK504585 A DK 504585A DK 504585 A DK504585 A DK 504585A DK 504585 A DK504585 A DK 504585A DK 504585 A DK504585 A DK 504585A
Authority
DK
Denmark
Prior art keywords
gene
genes
regie
subtilis
plasmids
Prior art date
Application number
DK504585A
Other languages
English (en)
Other versions
DK504585D0 (da
DK173773B1 (da
Inventor
Jan Kok
Jan Maat
Gerard Venema
Josephus Mauritius Barn Vossen
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Nv filed Critical Unilever Nv
Publication of DK504585D0 publication Critical patent/DK504585D0/da
Publication of DK504585A publication Critical patent/DK504585A/da
Application granted granted Critical
Publication of DK173773B1 publication Critical patent/DK173773B1/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Dairy Products (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DK198505045A 1984-03-02 1985-11-01 Rekombinante plasmider, bakterier med indhold heraf og fremgangsmåde til fremstilling af fermenterede næringsmiddelprodukter, dyrefoder eller proteiner ved hjælp af sådanne bakterier DK173773B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
NL8400687 1984-03-02
NL8400687A NL8400687A (nl) 1984-03-02 1984-03-02 Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit.
PCT/EP1985/000077 WO1985003945A1 (en) 1984-03-02 1985-03-04 Recombinant plasmids; bacteria containing such recombinant plasmids; process for preparing fermented food products, animal feedstuffs, ingredients thereof or proteins using such bacteria
EP8500077 1985-03-04

Publications (3)

Publication Number Publication Date
DK504585D0 DK504585D0 (da) 1985-11-01
DK504585A true DK504585A (da) 1985-11-01
DK173773B1 DK173773B1 (da) 2001-10-01

Family

ID=19843593

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198505045A DK173773B1 (da) 1984-03-02 1985-11-01 Rekombinante plasmider, bakterier med indhold heraf og fremgangsmåde til fremstilling af fermenterede næringsmiddelprodukter, dyrefoder eller proteiner ved hjælp af sådanne bakterier

Country Status (7)

Country Link
EP (1) EP0157441B1 (da)
JP (1) JP2711258B2 (da)
AT (1) ATE72832T1 (da)
DE (1) DE3585415D1 (da)
DK (1) DK173773B1 (da)
NL (1) NL8400687A (da)
WO (1) WO1985003945A1 (da)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8503316A (nl) * 1985-11-29 1987-06-16 Stichting Nl I Zuivelonderzoek Werkwijze voor het bereiden van proteinen met behulp van gastheer-organismen, in het bijzonder melkzuurbacterien, welke recombinant dna-plasmiden met een hoge replicon-aktiviteit en een hoge promotor-aktiviteit voor het betreffende proteine-coderende gen bevatten, de van dergelijke recombinant dna-plasmiden voorziene gastheerorganismen, de recombinant dna-plasmiden zelve, de replicon-aktiviteit en promotor-aktiviteit coderende dna-fragmenten, alsmede de verkregen proteinen.
ES2061482T3 (es) * 1986-05-02 1994-12-16 Gist Brocades Nv Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus.
CA1339101C (en) * 1986-06-03 1997-07-29 Nicolaas Overbeeke Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods
NL8700767A (nl) * 1987-04-01 1988-11-01 Groningen Science Park Dipeptidase, isolering daarvan uit melkzuurbacterien, antilichamen tegen het dipeptidase, gebruik van het dipeptidase en van de antilichamen daartegen.
NL8801529A (nl) * 1988-06-15 1990-01-02 Nl Zuivelonderzoek Inst Werkwijze voor het selecteren en stabiel handhaven van recombinant dna in melkzuurbacterien.
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
US5242821A (en) * 1989-07-10 1993-09-07 Valio, Finnish Co-Operative Dairies' Association Lactococcus promoter and signal sequences for expression in bacteria
NL8902010A (nl) * 1989-08-04 1991-03-01 Nl Zuivelonderzoek Inst Dna-fragment met ten minste een protease-gen, een dergelijk dna-fragment bevattende cloneringsvektor, een gastheercel getransformeerd met een dusdanig opgebouwde cloneringsvektor, alsmede de met dergelijke gastheercellen geproduceerde proteasen resp. bereide produkten zoals voedingsmiddelen.
GB9006400D0 (en) * 1990-03-22 1990-05-23 Ciba Geigy Ag Bacterial vectors
EP0487159A1 (en) * 1990-11-23 1992-05-27 Unilever N.V. A food-grade vector suitable for transforming a food-grade host cell, use of said vector for transforming food-grade host cells, and use of said transformed cells in biotransformation processes
EP0972838B1 (en) * 1998-07-15 2004-09-15 Roche Diagnostics GmbH Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy
US6291245B1 (en) 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
IL66065A (en) * 1981-06-22 1989-06-30 Lilly Co Eli Recombinant dna cloning vectors and the e.coli transformants thereof
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos

Also Published As

Publication number Publication date
DK504585D0 (da) 1985-11-01
EP0157441A1 (en) 1985-10-09
JP2711258B2 (ja) 1998-02-10
DE3585415D1 (de) 1992-04-02
DK173773B1 (da) 2001-10-01
WO1985003945A1 (en) 1985-09-12
EP0157441B1 (en) 1992-02-26
JPS62500280A (ja) 1987-02-05
NL8400687A (nl) 1985-10-01
ATE72832T1 (de) 1992-03-15

Similar Documents

Publication Publication Date Title
Rinas et al. Protein compositional analysis of inclusion bodies produced in recombinant Escherichia coli
DK504585A (da) Rekombinante plasmider, bakterier med indhold heraf, fremgangsmaade til fremstilling af fermenterede foedevareprodukter, dyrefoder, bestanddele hertil eller proteiner ved hjaelp af saadanne bakterier
Vos et al. A maturation protein is essential for production of active forms of Lactococcus lactis SK11 serine proteinase located in or secreted from the cell envelope
Harlander Transformation of Streptococcus lactis by electroporation.
ATE39819T1 (de) Konservierung von lebensmitteln mit streptococcus lactis subspezie diacetylactis zur verhinderung der vergaerung von laktose.
EP0088622B1 (en) Animal interferons, processes involved in their production, compositions containing them, DNA sequences coding therefor and expression vehicles containing such sequences and cells transformed thereby
NZ210300A (en) Preparation of hyaluronic acid from bacterial culture
GB2100738A (en) Recombinant dna cloning vectors and the eukaryotic and prokaryotic transformants thereof
Law et al. Amino acid nutrition of some commercial cheese starters in relation to their growth in peptone-supplemented whey media
WO1984004535A1 (en) Heat regulated production of selected and fused proteins in yeast
DE69527126T2 (de) Verfahren zur Kontrolle der Genexpression in Milchsäurebakterien
CA2072007A1 (en) Cloning Vector for Use in Lactic Acid Bacteria and a Method for Constructing the Same
EP0111814A3 (en) The cloning and expression of nucleotide sequences encoding bovine growth hormone
US5672477A (en) Streptococcus lactis plasmid encoding for mucoidness
US6133023A (en) Lactic acid bacterial regulatable expression system
EP0355036B1 (en) Process for selecting and maintaining recombinant DNA in lactic acid bacteria
Hill et al. A qualitative screening procedure for the detection of casein hydrolysis by bacteria, using sodium dodecyl sulphate polyacrylamide gel electrophoresis
Feirtag et al. Thermosensitive plasmid replication, temperature-sensitive host growth, and chromosomal plasmid integration conferred by Lactococcus lactis subsp. cremoris lactose plasmids in Lactococcus lactis subsp. lactis
EP0219034A2 (de) Verfahren zur Herstellung von mesophilen Mikroorganismen, die eine bei höherer Temperatur aktive D-Hydantoinase enthalten
Mercurio et al. Cellular localization and export of the soluble haemolysin of Vibrio cholerae El Tor
US5939317A (en) Use of a Sec-dependent secretion system for secreting proteins that are usually secreted by a Sec-independent secretion system, bacteria containing it and their use
Lau et al. Proteins of transcriptionally active and inactive chromatin from Friend erythroleukemia cells
Walsh et al. Restriction endonuclease analysis of the lactose plasmid in Streptococcus lactis ML3 and two recombinant lactose plasmids
EP0148552A1 (en) Streptomyces expression sequence and methods for expressing DNA sequences in streptomyces
US5019506A (en) Plasmid and uses thereof

Legal Events

Date Code Title Description
B1 Patent granted (law 1993)
PBP Patent lapsed

Country of ref document: DK