NL8400687A - Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit. - Google Patents

Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit. Download PDF

Info

Publication number: NL8400687A
Authority: NL; Netherlands
Prior art keywords: plasmid; lactic acid; gene; acid bacteria; recombinant plasmid
Prior art date: 1984-03-02

Application number

NL8400687A

Other languages

English (en)

Dutch (nl)

Original Assignee

Unilever Nv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-03-02

Filing date

1984-03-02

Publication date

1985-10-01

1984-03-02 Application filed by Unilever Nv filed Critical Unilever Nv

1984-03-02 Priority to NL8400687A priority Critical patent/NL8400687A/nl

1985-03-04 Priority to PCT/EP1985/000077 priority patent/WO1985003945A1/en

1985-03-04 Priority to JP60501464A priority patent/JP2711258B2/ja

1985-03-04 Priority to DE8585200316T priority patent/DE3585415D1/de

1985-03-04 Priority to EP19850200316 priority patent/EP0157441B1/en

1985-03-04 Priority to AT85200316T priority patent/ATE72832T1/de

1985-10-01 Publication of NL8400687A publication Critical patent/NL8400687A/nl

1985-11-01 Priority to DK198505045A priority patent/DK173773B1/da

1993-10-12 Priority to US08/135,133 priority patent/US5683909A/en

Links

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108090000623 proteins and genes Proteins 0.000 title claims abstract description 38
241000894006 Bacteria Species 0.000 title claims abstract description 36
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239000008267 milk Substances 0.000 title description 2
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JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 59
235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 37
241000588724 Escherichia coli Species 0.000 claims abstract description 30
239000004310 lactic acid Substances 0.000 claims abstract description 30
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241000194034 Lactococcus lactis subsp. cremoris Species 0.000 claims abstract description 6
235000014962 Streptococcus cremoris Nutrition 0.000 claims abstract description 5
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239000012634 fragment Substances 0.000 claims description 37
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JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 description 2
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OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
101001053401 Arabidopsis thaliana Acid beta-fructofuranosidase 3, vacuolar Proteins 0.000 description 1
241000702005 Bacillus phage SPP1 Species 0.000 description 1
BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
102000012410 DNA Ligases Human genes 0.000 description 1
108010061982 DNA Ligases Proteins 0.000 description 1
108010076804 DNA Restriction Enzymes Proteins 0.000 description 1
238000007399 DNA isolation Methods 0.000 description 1
241000588722 Escherichia Species 0.000 description 1
240000001624 Espostoa lanata Species 0.000 description 1
235000009161 Espostoa lanata Nutrition 0.000 description 1
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102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
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238000002105 Southern blotting Methods 0.000 description 1
241000191967 Staphylococcus aureus Species 0.000 description 1
241000194017 Streptococcus Species 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
239000008051 TBE buffer Substances 0.000 description 1
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KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
239000004327 boric acid Substances 0.000 description 1
239000013611 chromosomal DNA Substances 0.000 description 1
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KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 1
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LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
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235000013618 yogurt Nutrition 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Dairy Products (AREA)
Fodder In General (AREA)
Peptides Or Proteins (AREA)
Enzymes And Modification Thereof (AREA)

NL8400687A 1984-03-02 1984-03-02 Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit. NL8400687A (nl)

Priority Applications (8)

Application Number	Priority Date	Filing Date	Title
NL8400687A NL8400687A (nl)	1984-03-02	1984-03-02	Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit.
PCT/EP1985/000077 WO1985003945A1 (en)	1984-03-02	1985-03-04	Recombinant plasmids; bacteria containing such recombinant plasmids; process for preparing fermented food products, animal feedstuffs, ingredients thereof or proteins using such bacteria
JP60501464A JP2711258B2 (ja)	1984-03-02	1985-03-04	組換プラスミド、当該プラスミドを含有する細菌及び当該細菌を使用して、酸酵食品、動物用飼料、同成分又は蛋白質を製造する方法
DE8585200316T DE3585415D1 (de)	1984-03-02	1985-03-04	Rekombinante plasmide, diese rekombinante plasmide enthaltende bakterien, verfahren zur herstellung von fermentierten nahrungsmitteln, tierfutter, bestandteile derseben oder proteinen unter verwendung dieser bakterien.
EP19850200316 EP0157441B1 (en)	1984-03-02	1985-03-04	Recombinant plasmids; bacteria containing such recombinant plasmids; process for preparing fermented food products, animal feedstuffs, ingredients thereof of proteins using such bacteria
AT85200316T ATE72832T1 (de)	1984-03-02	1985-03-04	Rekombinante plasmide, diese rekombinante plasmide enthaltende bakterien, verfahren zur herstellung von fermentierten nahrungsmitteln, tierfutter, bestandteile derseben oder proteinen unter verwendung dieser bakterien.
DK198505045A DK173773B1 (da)	1984-03-02	1985-11-01	Rekombinante plasmider, bakterier med indhold heraf og fremgangsmåde til fremstilling af fermenterede næringsmiddelprodukter, dyrefoder eller proteiner ved hjælp af sådanne bakterier
US08/135,133 US5683909A (en)	1984-03-02	1993-10-12	Plasmids replicatable in Bacillus subtilis, E. coli and lactic acid streptococcus bacteria

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
NL8400687		1984-03-02
NL8400687A NL8400687A (nl)	1984-03-02	1984-03-02	Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit.

Publications (1)

Publication Number	Publication Date
NL8400687A true NL8400687A (nl)	1985-10-01

Family

ID=19843593

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NL8400687A NL8400687A (nl)	1984-03-02	1984-03-02	Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit.

Country Status (7)

Country	Link
EP (1)	EP0157441B1 (da)
JP (1)	JP2711258B2 (da)
AT (1)	ATE72832T1 (da)
DE (1)	DE3585415D1 (da)
DK (1)	DK173773B1 (da)
NL (1)	NL8400687A (da)
WO (1)	WO1985003945A1 (da)

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NL8503316A (nl) *	1985-11-29	1987-06-16	Stichting Nl I Zuivelonderzoek	Werkwijze voor het bereiden van proteinen met behulp van gastheer-organismen, in het bijzonder melkzuurbacterien, welke recombinant dna-plasmiden met een hoge replicon-aktiviteit en een hoge promotor-aktiviteit voor het betreffende proteine-coderende gen bevatten, de van dergelijke recombinant dna-plasmiden voorziene gastheerorganismen, de recombinant dna-plasmiden zelve, de replicon-aktiviteit en promotor-aktiviteit coderende dna-fragmenten, alsmede de verkregen proteinen.
EP0244042B1 (en) *	1986-05-02	1994-08-17	Gist-Brocades N.V.	Secretory signal selection vectors for extracellular protein synthesis in bacilli
CA1339101C (en) *	1986-06-03	1997-07-29	Nicolaas Overbeeke	Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods
NL8700767A (nl) *	1987-04-01	1988-11-01	Groningen Science Park	Dipeptidase, isolering daarvan uit melkzuurbacterien, antilichamen tegen het dipeptidase, gebruik van het dipeptidase en van de antilichamen daartegen.
NL8801529A (nl) *	1988-06-15	1990-01-02	Nl Zuivelonderzoek Inst	Werkwijze voor het selecteren en stabiel handhaven van recombinant dna in melkzuurbacterien.
US5658871A (en) *	1989-07-07	1997-08-19	Lever Brothers Company, Division Of Conopco, Inc.	Microbial lipase muteins and detergent compositions comprising same
US5242821A (en) *	1989-07-10	1993-09-07	Valio, Finnish Co-Operative Dairies' Association	Lactococcus promoter and signal sequences for expression in bacteria
NL8902010A (nl) *	1989-08-04	1991-03-01	Nl Zuivelonderzoek Inst	Dna-fragment met ten minste een protease-gen, een dergelijk dna-fragment bevattende cloneringsvektor, een gastheercel getransformeerd met een dusdanig opgebouwde cloneringsvektor, alsmede de met dergelijke gastheercellen geproduceerde proteasen resp. bereide produkten zoals voedingsmiddelen.
GB9006400D0 (en) *	1990-03-22	1990-05-23	Ciba Geigy Ag	Bacterial vectors
EP0487159A1 (en) *	1990-11-23	1992-05-27	Unilever N.V.	A food-grade vector suitable for transforming a food-grade host cell, use of said vector for transforming food-grade host cells, and use of said transformed cells in biotransformation processes
EP0972838B1 (en) *	1998-07-15	2004-09-15	Roche Diagnostics GmbH	Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy
US6291245B1 (en)	1998-07-15	2001-09-18	Roche Diagnostics Gmbh	Host-vector system

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4666847A (en) *	1981-01-16	1987-05-19	Collaborative Research, Inc.	Recombinant DNA means and method
IL66065A (en) *	1981-06-22	1989-06-30	Lilly Co Eli	Recombinant dna cloning vectors and the e.coli transformants thereof
BR8205954A (pt) *	1981-10-14	1983-09-13	Unilever Nv	Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos

1984
- 1984-03-02 NL NL8400687A patent/NL8400687A/nl not_active Application Discontinuation
1985
- 1985-03-04 EP EP19850200316 patent/EP0157441B1/en not_active Expired - Lifetime
- 1985-03-04 DE DE8585200316T patent/DE3585415D1/de not_active Expired - Fee Related
- 1985-03-04 AT AT85200316T patent/ATE72832T1/de not_active IP Right Cessation
- 1985-03-04 WO PCT/EP1985/000077 patent/WO1985003945A1/en not_active Ceased
- 1985-03-04 JP JP60501464A patent/JP2711258B2/ja not_active Expired - Lifetime
- 1985-11-01 DK DK198505045A patent/DK173773B1/da not_active IP Right Cessation

Also Published As

Publication number	Publication date
JP2711258B2 (ja)	1998-02-10
JPS62500280A (ja)	1987-02-05
DK504585A (da)	1985-11-01
DE3585415D1 (de)	1992-04-02
DK173773B1 (da)	2001-10-01
EP0157441A1 (en)	1985-10-09
EP0157441B1 (en)	1992-02-26
WO1985003945A1 (en)	1985-09-12
DK504585D0 (da)	1985-11-01
ATE72832T1 (de)	1992-03-15

Legal Events

Date	Code	Title	Description
1985-10-01	A1B	A search report has been drawn up
1988-11-01	BV	The patent application has lapsed

Publication	Publication Date	Title
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Kang et al.	1998	Transcriptional analysis of the Bordetella alcaligin siderophore biosynthesis operon