DK591684A - Fremgangsmaade til detektering af en bestemt polynucleotidsekvens i en testproeve indeholdende enkeltstrengede nucleinsyrer samt reagenssystem til anvendelse ved fremgangsmaaden - Google Patents

Fremgangsmaade til detektering af en bestemt polynucleotidsekvens i en testproeve indeholdende enkeltstrengede nucleinsyrer samt reagenssystem til anvendelse ved fremgangsmaaden Download PDF

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Publication number
DK591684A
DK591684A DK591684A DK591684A DK591684A DK 591684 A DK591684 A DK 591684A DK 591684 A DK591684 A DK 591684A DK 591684 A DK591684 A DK 591684A DK 591684 A DK591684 A DK 591684A
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Prior art keywords
procedure
strength
detecting
nucleic acids
test sample
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DK591684A
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Danish (da)
English (en)
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DK591684D0 (da
Inventor
James P Albarella
Leslie H Anderson
Robert J Carrico
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Miles Lab
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Publication date
Priority claimed from US06/668,255 external-priority patent/US4743535A/en
Application filed by Miles Lab filed Critical Miles Lab
Publication of DK591684D0 publication Critical patent/DK591684D0/da
Publication of DK591684A publication Critical patent/DK591684A/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
DK591684A 1983-12-12 1984-12-11 Fremgangsmaade til detektering af en bestemt polynucleotidsekvens i en testproeve indeholdende enkeltstrengede nucleinsyrer samt reagenssystem til anvendelse ved fremgangsmaaden DK591684A (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US56042983A 1983-12-12 1983-12-12
US64585084A 1984-08-31 1984-08-31
US06/668,255 US4743535A (en) 1984-11-07 1984-11-07 Hybridization assay employing labeled probe and anti-hybrid

Publications (2)

Publication Number Publication Date
DK591684D0 DK591684D0 (da) 1984-12-11
DK591684A true DK591684A (da) 1985-06-13

Family

ID=27415823

Family Applications (1)

Application Number Title Priority Date Filing Date
DK591684A DK591684A (da) 1983-12-12 1984-12-11 Fremgangsmaade til detektering af en bestemt polynucleotidsekvens i en testproeve indeholdende enkeltstrengede nucleinsyrer samt reagenssystem til anvendelse ved fremgangsmaaden

Country Status (4)

Country Link
EP (1) EP0144913A3 (es)
DK (1) DK591684A (es)
ES (1) ES8607559A1 (es)
FI (1) FI844869A7 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
AU558846B2 (en) * 1985-06-21 1987-02-12 Miles Laboratories Inc. Solid-phase hydridization assay using anti-hybrid antibodies
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
US4760017A (en) * 1985-12-23 1988-07-26 E. I. Du Pont De Nemours And Company Arabinonucleic acid probes for DNA/RNA assays
US4822731A (en) * 1986-01-09 1989-04-18 Cetus Corporation Process for labeling single-stranded nucleic acids and hybridizaiton probes
FR2606154B1 (fr) * 1986-11-05 1990-05-11 Pasteur Institut Procede de detection et de dosage immunologique des acides nucleiques presents dans les milieux biologiques
FR2606155B1 (fr) * 1986-11-05 1990-07-20 Pasteur Institut Reactif immunologique pour la determination de la sequence d'un acide nucleique, son procede de preparation et methode immunologique de determination de la sequence d'un acide nucleique a l'aide de ce reactif
DE3639109A1 (de) * 1986-11-15 1988-05-19 Merck Patent Gmbh Verfahren zur bestimmung von nucleinsaeuren
IL85551A0 (en) * 1987-04-01 1988-08-31 Miles Inc Rapid hybridization assay and reagent system used therein
GB9114525D0 (en) * 1991-07-05 1991-08-21 Delnatte Sabine Homogeneous nucleic acids probe based tests and substances
CA2075858C (en) * 1991-08-15 1998-05-19 Scott J. Eisenbeis Detection of complementary nucleotide sequences
HU225970B1 (en) * 1991-10-11 2008-01-28 Acad Of Science Czech Republic Antiviral acyclic phosphonomethoxyalkyl-substituted alkenyl purine and pyrimidine derivatives, and pharmaceutical preparations containing them
CA2126952C (en) * 1994-06-28 2010-12-14 Sithian Pandian Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays
DE19534122A1 (de) * 1995-09-14 1997-03-20 Behringwerke Ag Homogener Gensondentest mit einem gegen das Label gerichteten Rezeptor
GB2324370B (en) * 1997-04-14 1999-03-03 Stuart Harbron Detection of hybrid double-stranded DNA with antibody after enzyme degradation of excess single-standed DNA
GB9822067D0 (en) * 1998-10-12 1998-12-02 Harbron Stuart Column-supported hybridisation assay in which excess probe is destroyed
US7560232B2 (en) * 2002-12-19 2009-07-14 Promega Corporation Methods of capturing, detecting and quantifying RNA:DNA hybrids and a modified RNase H useful therein
WO2012016936A1 (de) * 2010-07-31 2012-02-09 Aj Innuscreen Gmbh Nachweis spezifischer nukleinsäuresequenzen mittels fluoreszenz - quenching

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1219824A (en) * 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
AU9094982A (en) * 1982-02-01 1983-08-11 Miles Laboratories Inc. Photogenic labels to alleviate quenching in immuno assays
IL68506A (en) * 1982-05-04 1988-06-30 Syva Co Simultaneous calibration heterogeneous immunoassay method and device and kit for use therein
DE3362399D1 (en) * 1982-05-24 1986-04-10 Miles Lab Improved homogeneous binding assay method and reagent system, test kit and test device therefor
CA1231303A (en) * 1983-08-05 1988-01-12 Robert J. Carrico Detection of bacteria by nucleic acid hybridization

Also Published As

Publication number Publication date
FI844869L (fi) 1985-06-13
ES8607559A1 (es) 1986-05-16
FI844869A0 (fi) 1984-12-10
EP0144913A3 (en) 1986-08-20
EP0144913A2 (en) 1985-06-19
DK591684D0 (da) 1984-12-11
FI844869A7 (fi) 1985-06-13
ES538543A0 (es) 1986-05-16

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