ES8607559A1 - Un metodo para detectar una secuencia nucleotidica deteminada en una muestra - Google Patents

Un metodo para detectar una secuencia nucleotidica deteminada en una muestra

Info

Publication number
ES8607559A1
ES8607559A1 ES538543A ES538543A ES8607559A1 ES 8607559 A1 ES8607559 A1 ES 8607559A1 ES 538543 A ES538543 A ES 538543A ES 538543 A ES538543 A ES 538543A ES 8607559 A1 ES8607559 A1 ES 8607559A1
Authority
ES
Spain
Prior art keywords
hybrid
label
labeled probe
rna
hybridization assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES538543A
Other languages
English (en)
Other versions
ES538543A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/668,255 external-priority patent/US4743535A/en
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Publication of ES8607559A1 publication Critical patent/ES8607559A1/es
Publication of ES538543A0 publication Critical patent/ES538543A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

METODO PRA DETECTAR UNA SECUENCIA POLINUCLEOTIDICA DETERMINADA EN UNA MUESTRA A ENSAYAR QUE CONTIENE ACIDOS NUCLEICOS. COMPRENDE LAS ETAPAS DE: A) FORMAR HIBRIDOS QUE CONTIENEN EPITOPOS PARA UN REACTIVO ANTICUERPO QUE NO SE UNE SUSTANCIALMENTE A LOS ACIDOS NUCLEICOS MONOCATENARIOS; B) PONER EN CONTACTO CUALQUIER HIBRIDO FORMADO CON EL REACTIVO ANTICUERPO, PRODUCIENDO EL TRAZADOR EN LA SONDA MARCADA UNA RESPUESTA DETECTABLE QUE, CUANDO LA SONDA MARCADA ESTA COMPRENDIDA EN UN HIBRIDO QUE SE UNE AL REACTIVO ANTICUERPO, ES MENSURABLEMENTE DIFERENTE DE LA RESPUESTA OBTENIDA CUANDO LA SONDA MARCADA NO ESTA COMPRENDIDA EN DICHO HIBRIDO; Y C) MEDIR LA RESPUESTA DETECTABLE EN FUNCION DE LA PRESENCIA DE LA SECUENCIA QUE HA DE SER DETECTADA EN LA MUESTRA.
ES538543A 1983-12-12 1984-12-12 Un metodo para detectar una secuencia nucleotidica deteminada en una muestra Expired ES8607559A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US56042983A 1983-12-12 1983-12-12
US64585084A 1984-08-31 1984-08-31
US06/668,255 US4743535A (en) 1984-11-07 1984-11-07 Hybridization assay employing labeled probe and anti-hybrid

Publications (2)

Publication Number Publication Date
ES8607559A1 true ES8607559A1 (es) 1986-05-16
ES538543A0 ES538543A0 (es) 1986-05-16

Family

ID=27415823

Family Applications (1)

Application Number Title Priority Date Filing Date
ES538543A Expired ES8607559A1 (es) 1983-12-12 1984-12-12 Un metodo para detectar una secuencia nucleotidica deteminada en una muestra

Country Status (4)

Country Link
EP (1) EP0144913A3 (es)
DK (1) DK591684A (es)
ES (1) ES8607559A1 (es)
FI (1) FI844869L (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
AU558846B2 (en) * 1985-06-21 1987-02-12 Miles Laboratories Inc. Solid-phase hydridization assay using anti-hybrid antibodies
US4760017A (en) * 1985-12-23 1988-07-26 E. I. Du Pont De Nemours And Company Arabinonucleic acid probes for DNA/RNA assays
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
US4822731A (en) * 1986-01-09 1989-04-18 Cetus Corporation Process for labeling single-stranded nucleic acids and hybridizaiton probes
FR2606155B1 (fr) * 1986-11-05 1990-07-20 Pasteur Institut Reactif immunologique pour la determination de la sequence d'un acide nucleique, son procede de preparation et methode immunologique de determination de la sequence d'un acide nucleique a l'aide de ce reactif
FR2606154B1 (fr) * 1986-11-05 1990-05-11 Pasteur Institut Procede de detection et de dosage immunologique des acides nucleiques presents dans les milieux biologiques
DE3639109A1 (de) * 1986-11-15 1988-05-19 Merck Patent Gmbh Verfahren zur bestimmung von nucleinsaeuren
IL85551A0 (en) * 1987-04-01 1988-08-31 Miles Inc Rapid hybridization assay and reagent system used therein
GB9114525D0 (en) * 1991-07-05 1991-08-21 Delnatte Sabine Homogeneous nucleic acids probe based tests and substances
CA2075858C (en) * 1991-08-15 1998-05-19 Scott J. Eisenbeis Detection of complementary nucleotide sequences
CZ287745B6 (cs) * 1991-10-11 2001-01-17 Ústav organické chemie a biochemie AV ČR Acyklické fosfonomethoxyalkylsubstituované alkenylové a alkinylové deriváty purinu a pyrimidinu
CA2126952C (en) * 1994-06-28 2010-12-14 Sithian Pandian Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays
DE19534122A1 (de) * 1995-09-14 1997-03-20 Behringwerke Ag Homogener Gensondentest mit einem gegen das Label gerichteten Rezeptor
GB2324370B (en) 1997-04-14 1999-03-03 Stuart Harbron Detection of hybrid double-stranded DNA with antibody after enzyme degradation of excess single-standed DNA
GB9822067D0 (en) * 1998-10-12 1998-12-02 Harbron Stuart Column-supported hybridisation assay in which excess probe is destroyed
US7560232B2 (en) 2002-12-19 2009-07-14 Promega Corporation Methods of capturing, detecting and quantifying RNA:DNA hybrids and a modified RNase H useful therein
WO2012016936A1 (de) * 2010-07-31 2012-02-09 Aj Innuscreen Gmbh Nachweis spezifischer nukleinsäuresequenzen mittels fluoreszenz - quenching

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1219824A (en) * 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
AU9094982A (en) * 1982-02-01 1983-08-11 Miles Laboratories Inc. Photogenic labels to alleviate quenching in immuno assays
IL68506A (en) * 1982-05-04 1988-06-30 Syva Co Simultaneous calibration heterogeneous immunoassay method and device and kit for use therein
EP0095089B1 (en) * 1982-05-24 1986-03-05 Miles Laboratories, Inc. Improved homogeneous binding assay method and reagent system, test kit and test device therefor
CA1231303A (en) * 1983-08-05 1988-01-12 Robert J. Carrico Detection of bacteria by nucleic acid hybridization

Also Published As

Publication number Publication date
FI844869A0 (fi) 1984-12-10
EP0144913A2 (en) 1985-06-19
DK591684D0 (da) 1984-12-11
DK591684A (da) 1985-06-13
EP0144913A3 (en) 1986-08-20
FI844869A7 (fi) 1985-06-13
ES538543A0 (es) 1986-05-16
FI844869L (fi) 1985-06-13

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