EA200000202A1 - Рекомбинантная экспрессия c-пептида инсулина - Google Patents
Рекомбинантная экспрессия c-пептида инсулинаInfo
- Publication number
- EA200000202A1 EA200000202A1 EA200000202A EA200000202A EA200000202A1 EA 200000202 A1 EA200000202 A1 EA 200000202A1 EA 200000202 A EA200000202 A EA 200000202A EA 200000202 A EA200000202 A EA 200000202A EA 200000202 A1 EA200000202 A1 EA 200000202A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- insulin
- peptide
- expressed
- recombinant expression
- cleaved
- Prior art date
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title 2
- 102000004877 Insulin Human genes 0.000 title 1
- 108090001061 Insulin Proteins 0.000 title 1
- 229940125396 insulin Drugs 0.000 title 1
- 238000003259 recombinant expression Methods 0.000 title 1
- VOUAQYXWVJDEQY-QENPJCQMSA-N 33017-11-7 Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)CCC1 VOUAQYXWVJDEQY-QENPJCQMSA-N 0.000 abstract 4
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 239000013604 expression vector Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Diabetes (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Obesity (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Emergency Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
В настоящем изобретении предлагается способ получения C-пептида инсулина, при котором осуществляют экспрессию в клетке-хозяине мультимерного полипептида, содержащего множественные копии указанного C-пептида инсулина, и расщепляют указанный экспрессированный полипептид с высвобождением единичных копий C-пептида инсулина. Предлагаются также молекулы нуклеиновых кислот, векторы экспрессии и клетки-хозяева для использования в таком способе, а также мультимерный C-пептид инсулина, экспрессированный и расщепленный таким способом.Отчет о международном поиске был опубликован 1999.04.29.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9716790.2A GB9716790D0 (en) | 1997-08-07 | 1997-08-07 | Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof |
| PCT/GB1998/002382 WO1999007735A2 (en) | 1997-08-07 | 1998-08-07 | Recombinant expression of insulin c-peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA200000202A1 true EA200000202A1 (ru) | 2001-04-23 |
Family
ID=10817184
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200000202A EA200000202A1 (ru) | 1997-08-07 | 1998-08-07 | Рекомбинантная экспрессия c-пептида инсулина |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US6558924B1 (ru) |
| EP (1) | EP1001980A1 (ru) |
| JP (1) | JP2001512745A (ru) |
| KR (1) | KR20010022590A (ru) |
| CN (1) | CN1268141A (ru) |
| AU (1) | AU750685B2 (ru) |
| BG (1) | BG104161A (ru) |
| BR (1) | BR9811850A (ru) |
| CA (1) | CA2297382A1 (ru) |
| EA (1) | EA200000202A1 (ru) |
| EE (1) | EE200000072A (ru) |
| GB (1) | GB9716790D0 (ru) |
| HU (1) | HUP0004626A1 (ru) |
| IL (1) | IL134372A0 (ru) |
| IS (1) | IS5368A (ru) |
| NO (1) | NO20000575L (ru) |
| NZ (1) | NZ502450A (ru) |
| PL (1) | PL339085A1 (ru) |
| SK (1) | SK1532000A3 (ru) |
| TR (1) | TR200000288T2 (ru) |
| WO (1) | WO1999007735A2 (ru) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040005669A1 (en) * | 1997-08-07 | 2004-01-08 | Stefan Stahl | Recombinant expression of insulin C-peptide |
| FR2810675B1 (fr) * | 2000-06-22 | 2002-09-27 | Pf Medicament | Construction modifiee en aval du codon d'initiation pour la surexpression de proteines recombinantes |
| FR2810674A1 (fr) * | 2000-06-22 | 2001-12-28 | Pf Medicament | Construction modifiee en amont du codon d'initiation pour la surexpression de proteines recombinantes |
| AU2002233230B2 (en) * | 2000-12-20 | 2007-02-01 | F. Hoffmann-La Roche Ag | Erythropoietin conjugates |
| CN101044154A (zh) * | 2002-02-14 | 2007-09-26 | 威廉·J·鲁特 | 治疗宿主中切割的嵌合分子 |
| WO2004047871A2 (en) | 2002-11-26 | 2004-06-10 | Nobex Corporation | Modified naturetic compounds, conjugates, and uses thereof |
| US20050026826A1 (en) * | 2003-01-17 | 2005-02-03 | Margarethe Hoenig | Feline proinsulin, insulin and constituent peptides |
| CN1298742C (zh) * | 2003-06-03 | 2007-02-07 | 上海新药研究开发中心 | 一种适合于高效表达的融合蛋白及其生产方法 |
| WO2005087811A2 (en) * | 2004-03-10 | 2005-09-22 | Creighton University | Estrogen receptors and methods of use |
| KR20130115086A (ko) | 2010-05-17 | 2013-10-21 | 세빅스 인코포레이티드 | 페길화된 c-펩티드 |
| US8962553B2 (en) | 2011-11-17 | 2015-02-24 | Cebix Ab | Method of treating a diabetic subject having a microvascular impairment disorder by a pegylated C-peptide |
| SG11201405721VA (en) * | 2012-03-19 | 2014-10-30 | Madeleine Pharmaceuticals Ρτy Ltd | Method of producing a recombinant peptide |
| WO2016145389A1 (en) | 2015-03-12 | 2016-09-15 | Board Of Trustees Of Michigan State University | Compositions and methods for measuring c-peptide binding and diagnosing immune-mediated diseases |
| CN109705221B (zh) * | 2018-12-27 | 2021-03-09 | 美康生物科技股份有限公司 | C肽免疫原及其单克隆抗体对及该抗体对在c肽磁微粒化学发光免疫试剂中的应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5210872B2 (ru) | 1973-07-14 | 1977-03-26 | ||
| GB2068971B (en) | 1980-01-30 | 1983-06-08 | Searle & Co | Recombinant dna techniques |
| CA1213537A (en) | 1984-05-01 | 1986-11-04 | Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee | Polypeptide expression method |
| US5013653A (en) | 1987-03-20 | 1991-05-07 | Creative Biomolecules, Inc. | Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage |
| EP0305500B1 (en) | 1987-03-20 | 1994-11-09 | Creative Biomolecules, Inc. | Process for the purification of recombinant polypeptides |
| SE501169C2 (sv) | 1988-02-05 | 1994-11-28 | Aake Nygren | Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner |
| US5130236A (en) | 1989-12-07 | 1992-07-14 | Eli Lilly And Company | Process for producing des(64,65)-proinsulin |
| US5595887A (en) | 1990-07-16 | 1997-01-21 | Bionebraska, Inc. | Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment |
| GB9015825D0 (en) | 1990-07-18 | 1990-09-05 | Ciba Geigy Ag | In vitro processing of fusion proteins |
| JPH07108232B2 (ja) | 1990-10-09 | 1995-11-22 | エム・ディ・リサーチ株式会社 | ペプチド又は蛋白質の製造方法 |
| US5953418A (en) | 1995-06-14 | 1999-09-14 | David Hall | Providing selective data broadcast receiver addressability |
| WO1997023639A1 (en) | 1995-12-22 | 1997-07-03 | Toray Industries, Inc. | Process for producing biologically active fused proteins |
-
1997
- 1997-08-07 GB GBGB9716790.2A patent/GB9716790D0/en active Pending
-
1998
- 1998-08-07 SK SK153-2000A patent/SK1532000A3/sk unknown
- 1998-08-07 HU HU0004626A patent/HUP0004626A1/hu unknown
- 1998-08-07 EP EP98937689A patent/EP1001980A1/en not_active Withdrawn
- 1998-08-07 AU AU86407/98A patent/AU750685B2/en not_active Ceased
- 1998-08-07 US US09/485,286 patent/US6558924B1/en not_active Expired - Fee Related
- 1998-08-07 BR BR9811850-1A patent/BR9811850A/pt not_active Application Discontinuation
- 1998-08-07 PL PL98339085A patent/PL339085A1/xx not_active Application Discontinuation
- 1998-08-07 TR TR2000/00288T patent/TR200000288T2/xx unknown
- 1998-08-07 IL IL13437298A patent/IL134372A0/xx unknown
- 1998-08-07 WO PCT/GB1998/002382 patent/WO1999007735A2/en not_active Ceased
- 1998-08-07 CN CN98808075A patent/CN1268141A/zh active Pending
- 1998-08-07 KR KR1020007001182A patent/KR20010022590A/ko not_active Withdrawn
- 1998-08-07 NZ NZ502450A patent/NZ502450A/xx unknown
- 1998-08-07 JP JP2000506237A patent/JP2001512745A/ja active Pending
- 1998-08-07 EA EA200000202A patent/EA200000202A1/ru unknown
- 1998-08-07 EE EEP200000072A patent/EE200000072A/xx unknown
- 1998-08-07 CA CA002297382A patent/CA2297382A1/en not_active Abandoned
-
2000
- 2000-02-01 IS IS5368A patent/IS5368A/is unknown
- 2000-02-04 NO NO20000575A patent/NO20000575L/no not_active Application Discontinuation
- 2000-02-17 BG BG104161A patent/BG104161A/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| SK1532000A3 (en) | 2000-09-12 |
| WO1999007735A3 (en) | 1999-04-29 |
| EE200000072A (et) | 2000-10-16 |
| CA2297382A1 (en) | 1999-02-18 |
| NO20000575D0 (no) | 2000-02-04 |
| IS5368A (is) | 2000-02-01 |
| US6558924B1 (en) | 2003-05-06 |
| KR20010022590A (ko) | 2001-03-26 |
| HUP0004626A1 (hu) | 2001-04-28 |
| WO1999007735A2 (en) | 1999-02-18 |
| GB9716790D0 (en) | 1997-10-15 |
| EP1001980A1 (en) | 2000-05-24 |
| TR200000288T2 (tr) | 2000-06-21 |
| AU8640798A (en) | 1999-03-01 |
| JP2001512745A (ja) | 2001-08-28 |
| PL339085A1 (en) | 2000-12-04 |
| IL134372A0 (en) | 2001-04-30 |
| NO20000575L (no) | 2000-04-04 |
| BR9811850A (pt) | 2000-08-08 |
| NZ502450A (en) | 2002-10-25 |
| CN1268141A (zh) | 2000-09-27 |
| AU750685B2 (en) | 2002-07-25 |
| BG104161A (en) | 2000-12-29 |
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