EA200802140A1 - Жидкая композиция, содержащая аспарагиновую протеазу - Google Patents

Жидкая композиция, содержащая аспарагиновую протеазу

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Publication number
EA200802140A1
EA200802140A1 EA200802140A EA200802140A EA200802140A1 EA 200802140 A1 EA200802140 A1 EA 200802140A1 EA 200802140 A EA200802140 A EA 200802140A EA 200802140 A EA200802140 A EA 200802140A EA 200802140 A1 EA200802140 A1 EA 200802140A1
Authority
EA
Eurasian Patent Office
Prior art keywords
liquid composition
proteas
asparagin
composition containing
count
Prior art date
Application number
EA200802140A
Other languages
English (en)
Other versions
EA025412B1 (ru
Inventor
Андре Хаан Де
Милен Коссет
Маргот Элизабет Франсуаз Шонефельд-Бергманс
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36950288&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA200802140(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA200802140A1 publication Critical patent/EA200802140A1/ru
Publication of EA025412B1 publication Critical patent/EA025412B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326—Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6413—Aspartic endopeptidases (3.4.23)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Dairy Products (AREA)
  • Detergent Compositions (AREA)

Abstract

Настоящее изобретение обеспечивает жидкую композицию, включающую: (i) аспарагиновую протеазу, и (ii) неорганическую соль и/или полиспирт; композицию, в которой суммарная концентрация сорбата, бензоата и сложного эфира алкилпарагидроксибензоата составляет меньше чем 0,010 мол/л; стандартный чашечный счет ≤100 в 1 мл; счет дрожжей ≤10 в 1 мл; и счет плесени ≤10 в 1 мл. Композиция может быть использована в качестве коагулянта в производстве сыра.
EA200802140A 2006-04-13 2007-04-12 Жидкая композиция, содержащая аспарагиновую протеазу EA025412B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US79144706P 2006-04-13 2006-04-13
EP06112649 2006-04-13
PCT/EP2007/053556 WO2007118838A1 (en) 2006-04-13 2007-04-12 Liquid composition comprising an aspartic protease

Publications (2)

Publication Number Publication Date
EA200802140A1 true EA200802140A1 (ru) 2009-02-27
EA025412B1 EA025412B1 (ru) 2016-12-30

Family

ID=36950288

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200802140A EA025412B1 (ru) 2006-04-13 2007-04-12 Жидкая композиция, содержащая аспарагиновую протеазу

Country Status (12)

Country Link
US (1) US20100009033A1 (ru)
EP (2) EP2004820A1 (ru)
JP (1) JP5557242B2 (ru)
CN (1) CN101421397A (ru)
BR (1) BRPI0709829A2 (ru)
DK (1) DK2333056T3 (ru)
EA (1) EA025412B1 (ru)
ES (1) ES2478872T3 (ru)
MX (1) MX2008013182A (ru)
NZ (1) NZ571699A (ru)
PL (1) PL2333056T3 (ru)
WO (1) WO2007118838A1 (ru)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5307013B2 (ja) 2006-09-21 2013-10-02 ヴェレニウム コーポレイション フィターゼ、フィターゼをコードする核酸、並びにフィターゼを製造および使用する方法
CN102325872B (zh) * 2009-02-20 2014-09-10 丹尼斯科美国公司 发酵肉汤制剂
CN101870967B (zh) * 2010-07-22 2012-05-23 安泰生物工程股份有限公司 半连续发酵生产微生物凝乳酶的方法
WO2012127005A1 (en) 2011-03-22 2012-09-27 Dsm Ip Assets B.V. Chymosin formulation
CN103773751A (zh) * 2012-10-22 2014-05-07 丰益(上海)生物技术研发中心有限公司 脂肪酶稳定剂
RU2684457C2 (ru) 2013-07-18 2019-04-09 Кхр. Хансен А/С Композиция молокосвертывающего фермента аспарагиновой протеазы
CN103740685B (zh) * 2013-10-11 2015-04-15 孟乙 利用米黑毛霉生产凝乳酶的方法
CN104480095A (zh) * 2014-11-27 2015-04-01 广西大学 一种酶活力稳定的液态木瓜蛋白酶制剂
JP2017029129A (ja) * 2015-07-30 2017-02-09 三菱化学フーズ株式会社 プロテアーゼの活性維持方法およびプロテアーゼ溶液
GB201520912D0 (en) 2015-11-26 2016-01-13 Csk Food Enrichment Bv Liquid composition comprising an aspartic protease having improved enzyme stability
WO2017089613A1 (en) * 2015-11-26 2017-06-01 Csk Food Enrichment B.V. Liquid composition comprising an aspartic protease having improved enzyme stability
WO2023237360A1 (en) 2022-06-07 2023-12-14 Chr. Hansen A/S Improving filterability of lactase by adding anions selected from malate, tartrate, citrate, gluconate, edta or combinations thereof and sterile filtered lactase product obtained
WO2024189187A1 (en) 2023-03-15 2024-09-19 Dsm Ip Assets B.V. Novel process for producing a fermented milk product and new fermented milk product so produced

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK110031A (ru) 1965-12-02
US3763010A (en) * 1971-06-10 1973-10-02 Baxter Laboratories Inc Stabilized microbial rennet
US4348482A (en) * 1978-05-22 1982-09-07 Miles Laboratories, Inc. Process for decreasing the thermal stability of microbial rennet
NO302485B1 (no) 1987-07-28 1998-03-09 Gist Brocades Bv Fremgangsmåte for fremstilling av et polypeptid av interesse i en Klyveromyces vertscelle, transformert Klyveromyces vertscelle og anvendelse derav, plasmid og DNA konstruksjon
US4853329A (en) * 1988-06-27 1989-08-01 Miles Inc. Stabilization of microbial rennet
CA2058453C (en) 1989-06-13 1999-06-01 Henry G. Heinsohn Chymosin recovery and purification
DK47493D0 (da) 1993-04-27 1993-04-27 Novo Nordisk As Fremgangsmaade til fremstilling af ost
JP2739704B2 (ja) * 1993-08-11 1998-04-15 アサヒビール株式会社 飲食品添加用液状パパイン組成物
DE69533986D1 (de) * 1994-05-03 2005-03-10 Chr Hansen As Horsholm Verfahren zur trennung milchgerinnender enzyme
JPH1169973A (ja) * 1997-08-29 1999-03-16 Kao Corp 酵素の安定化方法
JP2002153269A (ja) * 2000-11-24 2002-05-28 Cosmo Products Kk プロテアーゼ溶液の安定化方法および皮膚化粧料
SE0004808D0 (sv) 2000-12-20 2000-12-20 Apbiotech Ab Method for the purification of an enzyme
EP1448768B1 (en) * 2001-12-19 2006-03-08 DSM IP Assets B.V. Method for the inactivation of amylase in the presence of protease
EP1365019B1 (en) 2002-05-21 2006-07-26 DSM IP Assets B.V. Fermentative production of microbial milk clotting protease
WO2003100048A1 (en) 2002-05-29 2003-12-04 Dsm Ip Assets B.V. Method for the purification of microbial protease

Also Published As

Publication number Publication date
EP2004820A1 (en) 2008-12-24
JP5557242B2 (ja) 2014-07-23
WO2007118838A1 (en) 2007-10-25
EP2333056B1 (en) 2014-05-07
NZ571699A (en) 2011-07-29
EP2333056A1 (en) 2011-06-15
JP2009533036A (ja) 2009-09-17
BRPI0709829A2 (pt) 2011-07-26
EA025412B1 (ru) 2016-12-30
MX2008013182A (es) 2008-10-27
CN101421397A (zh) 2009-04-29
ES2478872T3 (es) 2014-07-23
DK2333056T3 (da) 2014-08-04
PL2333056T3 (pl) 2014-09-30
US20100009033A1 (en) 2010-01-14

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU