ECSP014147A - Tecnica de trasfecciòn intensificada - Google Patents

Tecnica de trasfecciòn intensificada

Info

Publication number
ECSP014147A
ECSP014147A EC2001004147A ECSP014147A ECSP014147A EC SP014147 A ECSP014147 A EC SP014147A EC 2001004147 A EC2001004147 A EC 2001004147A EC SP014147 A ECSP014147 A EC SP014147A EC SP014147 A ECSP014147 A EC SP014147A
Authority
EC
Ecuador
Prior art keywords
protein
intensified
transfer technique
plasmids
episomal maintenance
Prior art date
Application number
EC2001004147A
Other languages
English (en)
Inventor
Myung-Sam Cho
Helena Yee
Original Assignee
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Corp filed Critical Bayer Corp
Publication of ECSP014147A publication Critical patent/ECSP014147A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
    • C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/30—Vector systems having a special element relevant for transcription being an enhancer not forming part of the promoter region
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un sistema vector de expresión génica en células de mamíferos que incluyen una pluralidad de plámidos y comprede: a) un sistema de mantenimiento episomal, b)un fuerte protor/intensificador, c)un complejo de transactivaciòn de proteinas y d) ADN que codifica pra la proteína heteróloga. Los complejos de mantenimiento episomal y de trasactivación de proteínas pueden incluir subelementos situados en el mismo o en diferentes plásmidos dentro del sistema de expresión celular.
EC2001004147A 2000-09-28 2001-09-27 Tecnica de trasfecciòn intensificada ECSP014147A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US67584700A 2000-09-28 2000-09-28

Publications (1)

Publication Number Publication Date
ECSP014147A true ECSP014147A (es) 2002-05-23

Family

ID=24712193

Family Applications (1)

Application Number Title Priority Date Filing Date
EC2001004147A ECSP014147A (es) 2000-09-28 2001-09-27 Tecnica de trasfecciòn intensificada

Country Status (18)

Country Link
EP (1) EP1356069B1 (es)
JP (1) JP5022555B2 (es)
AR (1) AR035495A1 (es)
AU (1) AU2001289075A1 (es)
CA (1) CA2420771C (es)
CO (1) CO5270027A1 (es)
DE (1) DE60119386T2 (es)
DO (1) DOP2001000254A (es)
EC (1) ECSP014147A (es)
ES (1) ES2263652T3 (es)
GT (1) GT200100194A (es)
HN (1) HN2001000215A (es)
MY (1) MY139948A (es)
PE (1) PE20030412A1 (es)
SV (1) SV2002000640A (es)
TW (1) TWI239354B (es)
UY (1) UY26947A1 (es)
WO (1) WO2002027005A2 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006121995A (ja) * 2004-10-29 2006-05-18 Tokyo Medical & Dental Univ Ｅｂｎａ１変異体
EP1945781B1 (en) 2005-10-28 2014-03-05 F.Hoffmann-La Roche Ag Protein expression in rodent cells
WO2011033005A2 (en) 2009-09-15 2011-03-24 Medimmune Limited Cells for transient expression and uses thereof
CN104995299B (zh) * 2012-12-11 2020-02-28 宝生物工程株式会社 表达盒

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9003010D0 (en) * 1990-02-09 1990-04-04 Glaxo Group Ltd Gene expression in yeast cells
WO1998000695A2 (en) * 1996-07-03 1998-01-08 Massachusetts Institute Of Technology Tat-sf: cofactor for stimulation of transcriptional elongation by hiv-1 tat
US6465251B1 (en) * 1996-11-13 2002-10-15 Dana-Farber Cancer Institute, Inc. Method of promoting b-cell proliferation and activation with CD40 ligand and cyclosporin
DZ2788A1 (fr) * 1998-05-15 2003-12-01 Bayer Ag Agonistes et antagonistes selectifs à IL-2.
IT1302403B1 (it) * 1998-11-06 2000-09-05 Angeletti P Ist Richerche Bio Cellule per la produzione di vettori adenovirali difettivi, metodoper la loro preparazione e loro uso.
US6136599A (en) * 1998-12-10 2000-10-24 Bayer Corporation Human hybrid host cell for mammalian gene expression

Also Published As

Publication number Publication date
JP2004509642A (ja) 2004-04-02
DE60119386D1 (de) 2006-06-08
WO2002027005A3 (en) 2003-07-31
CA2420771A1 (en) 2002-04-04
CO5270027A1 (es) 2003-04-30
EP1356069B1 (en) 2006-05-03
CA2420771C (en) 2010-12-21
MY139948A (en) 2009-11-30
JP5022555B2 (ja) 2012-09-12
TWI239354B (en) 2005-09-11
HN2001000215A (es) 2003-11-11
SV2002000640A (es) 2002-10-24
PE20030412A1 (es) 2003-06-26
DE60119386T2 (de) 2007-04-19
WO2002027005A9 (en) 2003-02-06
GT200100194A (es) 2002-05-16
WO2002027005A2 (en) 2002-04-04
UY26947A1 (es) 2002-04-26
AU2001289075A1 (en) 2002-04-08
DOP2001000254A (es) 2002-06-15
AR035495A1 (es) 2004-06-02
ES2263652T3 (es) 2006-12-16
EP1356069A2 (en) 2003-10-29

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