ECSP099814A - Una rosa que contiene flavona y malvidina y metodo para su produccion - Google Patents

Una rosa que contiene flavona y malvidina y metodo para su produccion

Info

Publication number
ECSP099814A
ECSP099814A EC2009009814A ECSP099814A ECSP099814A EC SP099814 A ECSP099814 A EC SP099814A EC 2009009814 A EC2009009814 A EC 2009009814A EC SP099814 A ECSP099814 A EC SP099814A EC SP099814 A ECSP099814 A EC SP099814A
Authority
EC
Ecuador
Prior art keywords
gene
flavone
hydroxylase
rose
scrophulariaceae family
Prior art date
Application number
EC2009009814A
Other languages
English (en)
Inventor
Yoshikazu Tanaka
Yukihisa Katsumoto
Masako Mizutani
Yuko Fukui
Junichi Togami
Noriko Nakamura
Original Assignee
Int Flower Dev Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Int Flower Dev Pty Ltd filed Critical Int Flower Dev Pty Ltd
Publication of ECSP099814A publication Critical patent/ECSP099814A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11022—Flavone synthase (1.14.11.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13088—Flavonoid 3',5'-hydroxylase (1.14.13.88)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

El invento proporciona una rosa caracterizada por comprender una flavona y malvidina añadida a través de un método de modificación genética. La flavona y malvidina se producen típicamente por expresión de un gen transferido de flavona sintasa, gen del flavonoide 3', 5'-hidroxilasa del pensamiento y gen de antocianidina metiltransferasa. El gen de síntesis de la flavona es, por ejemplo, un gen de síntesis de flavona de la familia Scrophulariaceae, y específicamente podría ser el gen de flavona sintasa del diente de león de la familia Scrophulariaceae, o el gen de flavona sintasa de torenia de la familia Scrophulariaceae. El gen del flavonoide 3', 5'-hidroxilasa es, por ejemplo, el gen del flavonoide 3', 5'-hidroxilasa del pensamiento. El gen de antocianidina metiltransferasa es, por ejemplo, el gen metiltransferasa de torenia de la familia Scrophulariaceae.
EC2009009814A 2007-06-20 2009-12-18 Una rosa que contiene flavona y malvidina y metodo para su produccion ECSP099814A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2007162959 2007-06-20

Publications (1)

Publication Number Publication Date
ECSP099814A true ECSP099814A (es) 2010-02-26

Family

ID=40156355

Family Applications (1)

Application Number Title Priority Date Filing Date
EC2009009814A ECSP099814A (es) 2007-06-20 2009-12-18 Una rosa que contiene flavona y malvidina y metodo para su produccion

Country Status (15)

Country Link
US (1) US20100281575A1 (es)
EP (1) EP2163619B1 (es)
JP (1) JP5057349B2 (es)
KR (1) KR101168421B1 (es)
CN (1) CN101679972B (es)
AU (1) AU2008264434B2 (es)
BR (1) BRPI0813127A8 (es)
CA (1) CA2690754C (es)
CO (1) CO6170374A2 (es)
EC (1) ECSP099814A (es)
ES (1) ES2385416T3 (es)
IL (1) IL202818A (es)
RU (1) RU2463348C2 (es)
TW (1) TWI440430B (es)
WO (1) WO2008156206A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008264439A1 (en) * 2007-06-20 2008-12-24 Suntory Holdings Limited Rose containing flavone, and method for production thereof
EP2159282A4 (en) * 2007-06-20 2010-07-28 Int Flower Dev Pty Ltd ROSE-FLAVON AND DELPHINIDINE AND METHOD OF PREPARING THEREOF
JP5099653B2 (ja) * 2009-03-27 2012-12-19 サントリーホールディングス株式会社 青いバラに含まれる新規化合物
WO2010122850A1 (ja) 2009-04-24 2010-10-28 独立行政法人農業・食品産業技術総合研究機構 修飾されたアントシアニンを花弁に含有するキク植物を生産する方法
CN105349553B (zh) * 2015-12-17 2018-09-07 海南大学 一种花色调节基因VwMYB29及其应用
CA3018690A1 (en) * 2016-03-31 2017-10-05 Suntory Holdings Limited Plant having blue flower color and breeding method therefor
CN106754989B (zh) * 2016-12-21 2020-04-21 广东药科大学 布渣叶黄烷酮-2-羟基化酶及其编码基因与应用
JP7246314B2 (ja) * 2017-10-03 2023-03-27 サントリーホールディングス株式会社 青系花色を有する形質転換植物及びその作出方法
KR102010833B1 (ko) * 2017-11-08 2019-08-16 대한민국 화훼식물의 꽃잎, 암술기관 및 화심 색 변형 기능 매발톱꽃 유래 신규 유전자 및 이의 용도
WO2020203940A1 (ja) * 2019-03-29 2020-10-08 サントリーホールディングス株式会社 フラボン７－ｏ－メチル基転移酵素遺伝子及びその使用
WO2021065749A1 (ja) 2019-10-01 2021-04-08 サントリーホールディングス株式会社 ソバ由来ｃ－配糖化酵素遺伝子及びその使用
EP4249597A4 (en) * 2020-11-18 2024-10-02 Suntory Holdings Limited FLAVON 4'-O METHYLTRANSFERASE GENE AND ITS USE
CN115094066B (zh) * 2022-05-06 2023-11-14 上海师范大学 一种调控月季花瓣颜色的基因RcTTG1及其应用
CN118557490A (zh) * 2024-08-01 2024-08-30 湖州嘉亨实业有限公司 一种玫瑰提取复合物的制备方法及组合物

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5480789A (en) * 1991-04-01 1996-01-02 Florigene Europe B.V. Genetically transformed rose plants and methods for their production
US5948955A (en) * 1991-07-13 1999-09-07 International Flower Developments Pty Ltd Transgenic plants having altered anthocyann levels
CA2130800C (en) * 1992-03-02 2002-05-14 Yasuhiro Kikuchi Plant genes encoding flavonoid-3',5'-hydroxylase
US20010007157A1 (en) * 1993-11-18 2001-07-05 Ebrahim Firoozabady Genetically transformed rose plants and methods for their production
US5568832A (en) * 1994-10-18 1996-10-29 Eddy; Zonell Skylight shade
JPH0970290A (ja) * 1995-02-17 1997-03-18 Suntory Ltd アシル基転移活性を有する蛋白質をコードする遺伝子
AUPN298895A0 (en) * 1995-05-16 1995-06-08 International Flower Developments Pty Ltd Transgenic plants exhibiting altered flower colour and methods for producing same
KR100816113B1 (ko) * 1998-01-22 2008-03-28 내셔날 리서치 카운실 오브 캐나다 식물의 이차 대사 화합물 함량의 변화 방법 및 그를 위한조성물
JP4368005B2 (ja) * 1999-01-29 2009-11-18 インターナショナル フラワー ディベロプメンツ プロプライアタリー リミティド フラボン合成酵素をコードする遺伝子
AUPS017402A0 (en) * 2002-01-25 2002-02-14 International Flower Developments Pty Ltd Genetic sequences and uses therefor
KR100440332B1 (ko) * 2002-06-28 2004-07-15 현대자동차주식회사 유압 회로용 투웨이 오리피스 첵밸브 장치
CN1678746B (zh) * 2002-08-30 2012-11-14 三得利控股株式会社 类黄酮3’,5’羟化酶基因序列及其用途
JP4378676B2 (ja) * 2003-06-03 2009-12-09 日本電気株式会社 電力制御方法および電力制御回路
AU2004264488B2 (en) * 2003-08-13 2008-10-02 Suntory Holdings Limited Process for producing rose with modified color
EP2159282A4 (en) * 2007-06-20 2010-07-28 Int Flower Dev Pty Ltd ROSE-FLAVON AND DELPHINIDINE AND METHOD OF PREPARING THEREOF

Also Published As

Publication number Publication date
CN101679972A (zh) 2010-03-24
JP5057349B2 (ja) 2012-10-24
EP2163619A4 (en) 2010-11-03
RU2010101626A (ru) 2011-07-27
KR20100017986A (ko) 2010-02-16
CA2690754C (en) 2014-09-23
JPWO2008156206A1 (ja) 2010-08-26
EP2163619B1 (en) 2012-05-16
KR101168421B1 (ko) 2012-07-25
BRPI0813127A2 (pt) 2014-10-14
CN101679972B (zh) 2013-09-04
TW200911109A (en) 2009-03-16
BRPI0813127A8 (pt) 2016-06-07
CA2690754A1 (en) 2008-12-24
ES2385416T3 (es) 2012-07-24
EP2163619A1 (en) 2010-03-17
TWI440430B (zh) 2014-06-11
AU2008264434B2 (en) 2012-03-08
IL202818A0 (en) 2011-08-01
IL202818A (en) 2015-08-31
AU2008264434A1 (en) 2008-12-24
CO6170374A2 (es) 2010-06-18
US20100281575A1 (en) 2010-11-04
RU2463348C2 (ru) 2012-10-10
WO2008156206A1 (ja) 2008-12-24

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