ECSP099814A - Una rosa que contiene flavona y malvidina y metodo para su produccion - Google Patents

Una rosa que contiene flavona y malvidina y metodo para su produccion

Info

Publication number
ECSP099814A
ECSP099814A EC2009009814A ECSP099814A ECSP099814A EC SP099814 A ECSP099814 A EC SP099814A EC 2009009814 A EC2009009814 A EC 2009009814A EC SP099814 A ECSP099814 A EC SP099814A EC SP099814 A ECSP099814 A EC SP099814A
Authority
EC
Ecuador
Prior art keywords
gene
flavone
hydroxylase
rose
scrophulariaceae family
Prior art date
Application number
EC2009009814A
Other languages
English (en)
Inventor
Yoshikazu Tanaka
Yukihisa Katsumoto
Masako Mizutani
Yuko Fukui
Junichi Togami
Noriko Nakamura
Original Assignee
Int Flower Dev Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Int Flower Dev Pty Ltd filed Critical Int Flower Dev Pty Ltd
Publication of ECSP099814A publication Critical patent/ECSP099814A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1003Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007Methyltransferases (general) (2.1.1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11022Flavone synthase (1.14.11.22)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13088Flavonoid 3',5'-hydroxylase (1.14.13.88)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y201/00Transferases transferring one-carbon groups (2.1)
    • C12Y201/01Methyltransferases (2.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

El invento proporciona una rosa caracterizada por comprender una flavona y malvidina añadida a través de un método de modificación genética. La flavona y malvidina se producen típicamente por expresión de un gen transferido de flavona sintasa, gen del flavonoide 3', 5'-hidroxilasa del pensamiento y gen de antocianidina metiltransferasa. El gen de síntesis de la flavona es, por ejemplo, un gen de síntesis de flavona de la familia Scrophulariaceae, y específicamente podría ser el gen de flavona sintasa del diente de león de la familia Scrophulariaceae, o el gen de flavona sintasa de torenia de la familia Scrophulariaceae. El gen del flavonoide 3', 5'-hidroxilasa es, por ejemplo, el gen del flavonoide 3', 5'-hidroxilasa del pensamiento. El gen de antocianidina metiltransferasa es, por ejemplo, el gen metiltransferasa de torenia de la familia Scrophulariaceae.
EC2009009814A 2007-06-20 2009-12-18 Una rosa que contiene flavona y malvidina y metodo para su produccion ECSP099814A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2007162959 2007-06-20

Publications (1)

Publication Number Publication Date
ECSP099814A true ECSP099814A (es) 2010-02-26

Family

ID=40156355

Family Applications (1)

Application Number Title Priority Date Filing Date
EC2009009814A ECSP099814A (es) 2007-06-20 2009-12-18 Una rosa que contiene flavona y malvidina y metodo para su produccion

Country Status (15)

Country Link
US (1) US20100281575A1 (es)
EP (1) EP2163619B1 (es)
JP (1) JP5057349B2 (es)
KR (1) KR101168421B1 (es)
CN (1) CN101679972B (es)
AU (1) AU2008264434B2 (es)
BR (1) BRPI0813127A8 (es)
CA (1) CA2690754C (es)
CO (1) CO6170374A2 (es)
EC (1) ECSP099814A (es)
ES (1) ES2385416T3 (es)
IL (1) IL202818A (es)
RU (1) RU2463348C2 (es)
TW (1) TWI440430B (es)
WO (1) WO2008156206A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100025555A (ko) * 2007-06-20 2010-03-09 인터내쇼날 플라워 디벨럽먼트 피티와이. 리미티드 플라본을 함유하는 장미 및 그 생산 방법
JPWO2008156214A1 (ja) * 2007-06-20 2010-08-26 インターナショナル フラワー ディベロプメンツ プロプライアタリー リミティド フラボン及びデルフィニジンを含むバラ及びその生産方法
EP2947083A1 (en) * 2009-03-27 2015-11-25 Suntory Holdings Limited Novel compound contained in blue rose
JP5697041B2 (ja) 2009-04-24 2015-04-08 独立行政法人農業・食品産業技術総合研究機構 修飾されたアントシアニンを花弁に含有するキク植物を生産する方法
CN105349553B (zh) * 2015-12-17 2018-09-07 海南大学 一种花色调节基因VwMYB29及其应用
WO2017169699A1 (ja) * 2016-03-31 2017-10-05 国立研究開発法人農業・食品産業技術総合研究機構 青系花色を有する植物及びその作出方法
CN106754989B (zh) * 2016-12-21 2020-04-21 广东药科大学 布渣叶黄烷酮-2-羟基化酶及其编码基因与应用
US20200283782A1 (en) * 2017-10-03 2020-09-10 Suntory Holdings Limited Transformed plant having blue flower color, and method for creating same
KR102010833B1 (ko) * 2017-11-08 2019-08-16 대한민국 화훼식물의 꽃잎, 암술기관 및 화심 색 변형 기능 매발톱꽃 유래 신규 유전자 및 이의 용도
CA3135580A1 (en) * 2019-03-29 2020-10-08 Suntory Holdings Limited Flavone 7-o-methyltransferase gene and use for same
JP7531507B2 (ja) * 2019-10-01 2024-08-09 サントリーホールディングス株式会社 ソバ由来c-配糖化酵素遺伝子及びその使用
CA3201507A1 (en) 2020-11-18 2022-05-27 Suntory Holdings Limited Flavone 4'-o-methyltransferase gene and use for same
CN115094066B (zh) * 2022-05-06 2023-11-14 上海师范大学 一种调控月季花瓣颜色的基因RcTTG1及其应用
CN118557490A (zh) * 2024-08-01 2024-08-30 湖州嘉亨实业有限公司 一种玫瑰提取复合物的制备方法及组合物

Family Cites Families (15)

* Cited by examiner, † Cited by third party
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US5480789A (en) * 1991-04-01 1996-01-02 Florigene Europe B.V. Genetically transformed rose plants and methods for their production
US5948955A (en) * 1991-07-13 1999-09-07 International Flower Developments Pty Ltd Transgenic plants having altered anthocyann levels
KR100245488B1 (ko) * 1992-03-02 2000-02-15 히라타 다다시 신규 식물 유전자
US20010007157A1 (en) * 1993-11-18 2001-07-05 Ebrahim Firoozabady Genetically transformed rose plants and methods for their production
US5568832A (en) * 1994-10-18 1996-10-29 Eddy; Zonell Skylight shade
JPH0970290A (ja) * 1995-02-17 1997-03-18 Suntory Ltd アシル基転移活性を有する蛋白質をコードする遺伝子
AUPN298895A0 (en) * 1995-05-16 1995-06-08 International Flower Developments Pty Ltd Transgenic plants exhibiting altered flower colour and methods for producing same
BR9907223A (pt) * 1998-01-22 2000-10-24 Ca Nat Research Council Métodos e composições para modificação dos nìveis de compostos metabólicos secundários em plantas
JP4368005B2 (ja) * 1999-01-29 2009-11-18 インターナショナル フラワー ディベロプメンツ プロプライアタリー リミティド フラボン合成酵素をコードする遺伝子
AUPS017402A0 (en) * 2002-01-25 2002-02-14 International Flower Developments Pty Ltd Genetic sequences and uses therefor
KR100440332B1 (ko) * 2002-06-28 2004-07-15 현대자동차주식회사 유압 회로용 투웨이 오리피스 첵밸브 장치
JP4798574B2 (ja) * 2002-08-30 2011-10-19 サントリーホールディングス株式会社 フラボノイド3’,5’ヒドロキシラーゼ遺伝子配列およびその使用法
JP4378676B2 (ja) * 2003-06-03 2009-12-09 日本電気株式会社 電力制御方法および電力制御回路
AU2004264488B2 (en) * 2003-08-13 2008-10-02 Suntory Holdings Limited Process for producing rose with modified color
JPWO2008156214A1 (ja) * 2007-06-20 2010-08-26 インターナショナル フラワー ディベロプメンツ プロプライアタリー リミティド フラボン及びデルフィニジンを含むバラ及びその生産方法

Also Published As

Publication number Publication date
RU2463348C2 (ru) 2012-10-10
WO2008156206A1 (ja) 2008-12-24
JP5057349B2 (ja) 2012-10-24
TWI440430B (zh) 2014-06-11
CN101679972B (zh) 2013-09-04
EP2163619A4 (en) 2010-11-03
KR20100017986A (ko) 2010-02-16
CA2690754A1 (en) 2008-12-24
EP2163619A1 (en) 2010-03-17
KR101168421B1 (ko) 2012-07-25
CO6170374A2 (es) 2010-06-18
CA2690754C (en) 2014-09-23
EP2163619B1 (en) 2012-05-16
BRPI0813127A8 (pt) 2016-06-07
IL202818A0 (en) 2011-08-01
IL202818A (en) 2015-08-31
CN101679972A (zh) 2010-03-24
AU2008264434A1 (en) 2008-12-24
AU2008264434B2 (en) 2012-03-08
JPWO2008156206A1 (ja) 2010-08-26
RU2010101626A (ru) 2011-07-27
ES2385416T3 (es) 2012-07-24
TW200911109A (en) 2009-03-16
US20100281575A1 (en) 2010-11-04
BRPI0813127A2 (pt) 2014-10-14

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