ECSP10010189A - Métodos para cocultivar células derivadas de sangre de cordón umbilical con células madre menstruales - Google Patents
Métodos para cocultivar células derivadas de sangre de cordón umbilical con células madre menstrualesInfo
- Publication number
- ECSP10010189A ECSP10010189A EC2010010189A ECSP10010189A ECSP10010189A EC SP10010189 A ECSP10010189 A EC SP10010189A EC 2010010189 A EC2010010189 A EC 2010010189A EC SP10010189 A ECSP10010189 A EC SP10010189A EC SP10010189 A ECSP10010189 A EC SP10010189A
- Authority
- EC
- Ecuador
- Prior art keywords
- cells
- cord blood
- blood cells
- methods
- umbilical cord
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 title abstract 12
- 238000000034 method Methods 0.000 title abstract 4
- 239000008280 blood Substances 0.000 title 1
- 210000004369 blood Anatomy 0.000 title 1
- 210000000130 stem cell Anatomy 0.000 title 1
- 210000003954 umbilical cord Anatomy 0.000 title 1
- 210000004700 fetal blood Anatomy 0.000 abstract 6
- 230000001332 colony forming effect Effects 0.000 abstract 3
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 abstract 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 abstract 2
- 230000000925 erythroid effect Effects 0.000 abstract 2
- 210000002540 macrophage Anatomy 0.000 abstract 2
- 230000002175 menstrual effect Effects 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 1
- 210000003643 myeloid progenitor cell Anatomy 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 238000009331 sowing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1352—Mesenchymal stem cells
- C12N2502/137—Blood-borne mesenchymal stem cells, e.g. Msc from umbilical cord blood
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1352—Mesenchymal stem cells
- C12N2502/1388—Mesenchymal stem cells from other natural sources
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/24—Genital tract cells, non-germinal cells from gonads
- C12N2502/243—Cells of the female genital tract, non-germinal ovarian cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se proveen métodos para obtener células expandidas de sangre de cordón umbilical humano que expresan CD34. Los métodos incluyen sembrar una cantidad suficiente de células de sangre de cordón con una cantidad suficiente de células menstruales, en condiciones de cocultivo adecuadas para promover la expansión de las células de sangre de cordón, y cocultivar las células de sangre de cordón con las células menstruales en condiciones de cultivo que soporten, al menos, dos o más duplicaciones de población de células de sangre de cordón. También se proveen métodos para desarrollar células expandidas de sangre de cordón umbilical humano para originar cualquiera de las unidades formadoras de colonias de células precursoras de linaje sanguíneo: unidad formadora de colonia granulocítica y macrofágica (CFU-GM), unidades formadoras de brotes eritroides (BFU-E), y unidad formadora de colonia granulocítica, eritroide, macrofágica, megacariocítica (CFU-GEMM). Las células expandidas pueden expresar CD34, SSEA-4, y HLA-II. También se proveen las composiciones de las células expandidas.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US145607P | 2007-10-31 | 2007-10-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ECSP10010189A true ECSP10010189A (es) | 2010-06-29 |
Family
ID=40591376
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EC2010010189A ECSP10010189A (es) | 2007-10-31 | 2010-05-19 | Métodos para cocultivar células derivadas de sangre de cordón umbilical con células madre menstruales |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US20090191628A1 (es) |
| EP (1) | EP2212418A4 (es) |
| JP (1) | JP2011501960A (es) |
| KR (1) | KR20100091192A (es) |
| CN (1) | CN101952416A (es) |
| AR (1) | AR069156A1 (es) |
| AU (1) | AU2008319284A1 (es) |
| BR (1) | BRPI0818152A2 (es) |
| CA (1) | CA2714777A1 (es) |
| CL (1) | CL2008003282A1 (es) |
| CR (1) | CR11409A (es) |
| EC (1) | ECSP10010189A (es) |
| IL (1) | IL205470A0 (es) |
| MX (1) | MX2010004914A (es) |
| NI (1) | NI201000077A (es) |
| PA (1) | PA8802801A1 (es) |
| TW (1) | TW200936148A (es) |
| WO (1) | WO2009058365A1 (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010056988A2 (en) | 2008-11-13 | 2010-05-20 | University Of South Florida | Methods of treating stroke using stem cell-like menstrual blood cells |
| DE102011101054A1 (de) | 2011-05-09 | 2012-02-16 | Bernhard Hildebrandt | Geräte und Vorrichtungen für das Implantationsverfahren für Endoprothesen, welches ein Systemzentrum und eine Systemachse sowie den dazu erforderlichen Gerätekomplex aus Fixiervorrichtung, Extensionsvorrichtung, Gliedmaßenhalterung und Steuergerät als Grundlage hat. |
| WO2013025189A1 (en) * | 2011-08-12 | 2013-02-21 | Cryo-Cell International, Inc. | Methods for co-culturing cord blood cells or cord tissue cells with menstrual multipotent cells |
| KR101348093B1 (ko) * | 2012-02-15 | 2014-01-16 | 고려대학교 산학협력단 | 공동 배양된 세포를 포함하는 섬유 및 그 제조방법 |
| US20160024473A1 (en) * | 2013-03-08 | 2016-01-28 | Cells For Cells | Menstrual stems cells for the efficient support and expansion of cd34+ cd133+ hematopoietic stem cells in vitro |
| JP6512759B2 (ja) | 2013-08-19 | 2019-05-15 | 国立研究開発法人国立循環器病研究センター | 羊膜間葉系細胞組成物の製造方法及び凍結保存方法、並びに治療剤 |
| JP6754459B2 (ja) * | 2013-08-19 | 2020-09-09 | 国立研究開発法人国立循環器病研究センター | 羊膜間葉系細胞組成物の製造方法及び凍結保存方法、並びに治療剤 |
| EP3593810B1 (en) | 2013-10-28 | 2021-06-23 | Biocardia, Inc. | Methods of measuring potential for therapeutic potency and defining dosages for autologous cell therapies |
| KR102676517B1 (ko) * | 2021-07-08 | 2024-06-20 | 박정원 | 생리혈로부터 줄기세포를 수득하는 방법 |
| WO2025185727A1 (en) * | 2024-03-08 | 2025-09-12 | The Chinese University Of Hong Kong | Normal saline medium prevents clotting of menstrual blood |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5192553A (en) * | 1987-11-12 | 1993-03-09 | Biocyte Corporation | Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of therapeutic use |
| US5840580A (en) * | 1990-05-01 | 1998-11-24 | Becton Dickinson And Company | Phenotypic characterization of the hematopoietic stem cell |
| US7410773B2 (en) * | 1995-02-02 | 2008-08-12 | Ghazi Jaswinder Dhoot | Method of preparing an undifferentiated cell |
| US7560280B2 (en) * | 2000-11-03 | 2009-07-14 | Kourion Therapeutics Gmbh | Human cord blood derived unrestricted somatic stem cells (USSC) |
| EP1876233A1 (en) * | 2005-01-24 | 2008-01-09 | Japan Health Sciences Foundation | Cells capable of differentiating into cardiac muscle cells |
| US20060182724A1 (en) * | 2005-02-15 | 2006-08-17 | Riordan Neil H | Method for expansion of stem cells |
| US20070041948A1 (en) * | 2005-07-20 | 2007-02-22 | Seoul National University Industry Foundation | Method for culturing and proliferating hematopoietic stem cells and progenitor cells using human endometrial cells |
| CN102851254A (zh) * | 2007-03-01 | 2013-01-02 | 干细胞国际公司 | 制备、分离及超低温保存子宫内膜/月经细胞 |
-
2008
- 2008-10-31 CA CA2714777A patent/CA2714777A1/en not_active Abandoned
- 2008-10-31 BR BRPI0818152-7A2A patent/BRPI0818152A2/pt not_active IP Right Cessation
- 2008-10-31 US US12/290,551 patent/US20090191628A1/en not_active Abandoned
- 2008-10-31 PA PA20088802801A patent/PA8802801A1/es unknown
- 2008-10-31 KR KR1020107011330A patent/KR20100091192A/ko not_active Withdrawn
- 2008-10-31 MX MX2010004914A patent/MX2010004914A/es not_active Application Discontinuation
- 2008-10-31 WO PCT/US2008/012376 patent/WO2009058365A1/en not_active Ceased
- 2008-10-31 AU AU2008319284A patent/AU2008319284A1/en not_active Abandoned
- 2008-10-31 TW TW097142288A patent/TW200936148A/zh unknown
- 2008-10-31 EP EP08845236A patent/EP2212418A4/en not_active Withdrawn
- 2008-10-31 AR ARP080104795A patent/AR069156A1/es not_active Application Discontinuation
- 2008-10-31 CN CN200880122741XA patent/CN101952416A/zh active Pending
- 2008-10-31 JP JP2010532061A patent/JP2011501960A/ja not_active Abandoned
- 2008-11-03 CL CL2008003282A patent/CL2008003282A1/es unknown
-
2010
- 2010-04-29 IL IL205470A patent/IL205470A0/en unknown
- 2010-04-30 CR CR11409A patent/CR11409A/es not_active Application Discontinuation
- 2010-04-30 NI NI201000077A patent/NI201000077A/es unknown
- 2010-05-19 EC EC2010010189A patent/ECSP10010189A/es unknown
-
2011
- 2011-08-12 US US13/209,123 patent/US20120208275A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AR069156A1 (es) | 2009-12-30 |
| CL2008003282A1 (es) | 2010-04-09 |
| CN101952416A (zh) | 2011-01-19 |
| PA8802801A1 (es) | 2009-06-23 |
| CA2714777A1 (en) | 2009-05-07 |
| MX2010004914A (es) | 2010-08-18 |
| CR11409A (es) | 2010-08-13 |
| AU2008319284A1 (en) | 2009-05-07 |
| BRPI0818152A2 (pt) | 2014-10-14 |
| EP2212418A1 (en) | 2010-08-04 |
| NI201000077A (es) | 2010-09-07 |
| JP2011501960A (ja) | 2011-01-20 |
| KR20100091192A (ko) | 2010-08-18 |
| IL205470A0 (en) | 2010-12-30 |
| EP2212418A4 (en) | 2010-12-08 |
| US20090191628A1 (en) | 2009-07-30 |
| TW200936148A (en) | 2009-09-01 |
| US20120208275A1 (en) | 2012-08-16 |
| WO2009058365A1 (en) | 2009-05-07 |
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