ECSP18054745A - Composiciones y métodos para el direccionamiento eficaz de transgenes - Google Patents

Composiciones y métodos para el direccionamiento eficaz de transgenes

Info

Publication number
ECSP18054745A
ECSP18054745A ECSENADI201854745A ECDI201854745A ECSP18054745A EC SP18054745 A ECSP18054745 A EC SP18054745A EC SENADI201854745 A ECSENADI201854745 A EC SENADI201854745A EC DI201854745 A ECDI201854745 A EC DI201854745A EC SP18054745 A ECSP18054745 A EC SP18054745A
Authority
EC
Ecuador
Prior art keywords
methods
plants
transgens
compositions
effective addressing
Prior art date
Application number
ECSENADI201854745A
Other languages
English (en)
Inventor
Michael Goley
Network Inc Brand
Qungang Qi
Kwan Thai
Clayton Larue
Aihua Shao
Sherry Leclere
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of ECSP18054745A publication Critical patent/ECSP18054745A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03004—Protoporphyrinogen oxidase (1.3.3.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08—Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La invención provee moléculas de ADN recombinante y construcciones útiles para proveer localización subcelular de transgén eficaz de proteínas en plantas transgénicas. También se provén moléculas de ADN recombinante y construcciones para conferir tolerancia o resistencia a herbicidas a plantas, así como plantas que presentan tolerancia a herbicidas y métodos para producir o utilizar dichas plantas.
ECSENADI201854745A 2015-12-21 2018-07-20 Composiciones y métodos para el direccionamiento eficaz de transgenes ECSP18054745A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562270180P 2015-12-21 2015-12-21
US201662364715P 2016-07-20 2016-07-20

Publications (1)

Publication Number Publication Date
ECSP18054745A true ECSP18054745A (es) 2018-08-31

Family

ID=59065936

Family Applications (1)

Application Number Title Priority Date Filing Date
ECSENADI201854745A ECSP18054745A (es) 2015-12-21 2018-07-20 Composiciones y métodos para el direccionamiento eficaz de transgenes

Country Status (25)

Country Link
US (1) US10563220B2 (es)
EP (2) EP4219729A3 (es)
JP (2) JP6961611B2 (es)
KR (1) KR102847177B1 (es)
CN (2) CN108463554B (es)
AU (2) AU2016377487B2 (es)
BR (1) BR102016029899A2 (es)
CA (1) CA2950803A1 (es)
CO (1) CO2018007568A2 (es)
DK (1) DK3394272T5 (es)
EC (1) ECSP18054745A (es)
ES (1) ES2941913T3 (es)
HR (1) HRP20230311T1 (es)
HU (1) HUE061632T2 (es)
MX (1) MX385151B (es)
PE (2) PE20181540A1 (es)
PH (1) PH12018501317B1 (es)
PL (1) PL3394272T3 (es)
PT (1) PT3394272T (es)
RS (1) RS64091B1 (es)
RU (1) RU2751238C2 (es)
UA (1) UA126554C2 (es)
UY (2) UY37040A (es)
WO (1) WO2017112589A1 (es)
ZA (1) ZA201803723B (es)

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* Cited by examiner, † Cited by third party
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UA126894C2 (uk) 2015-08-03 2023-02-22 Монсанто Текнолоджі Ллс Спосіб і композиція для стійкості рослин до гербіцидів
US10378023B2 (en) 2015-09-01 2019-08-13 Monsanto Technology Llc Methods and compositions for herbicide tolerance in plants
US10863743B2 (en) 2016-07-29 2020-12-15 Basf Se Method for controlling PPO resistant weeds
KR102587506B1 (ko) 2016-07-29 2023-10-11 몬산토 테크놀로지 엘엘씨 식물에서 유전자 발현을 위한 방법 및 조성물
CA3026528A1 (en) * 2017-12-15 2019-06-15 Monsanto Technology Llc Methods and compositions for ppo herbicide tolerance
WO2018043764A2 (en) * 2017-12-20 2018-03-08 Sumitomo Chemical Company, Limited Method for controlling harmful organisms in crops
CA3057917A1 (en) * 2018-10-16 2020-04-16 Monsanto Technology Llc Brassica event mon94100 and methods of use thereof
CN115336534A (zh) * 2021-05-12 2022-11-15 北京大北农生物技术有限公司 原卟啉原氧化酶的用途
WO2023044364A1 (en) 2021-09-15 2023-03-23 Enko Chem, Inc. Protoporphyrinogen oxidase inhibitors
JP2025503677A (ja) 2022-01-14 2025-02-04 エンコ ケム，インコーポレイテッド プロトポルフィリノーゲンオキシダーゼ阻害剤
US20240229063A1 (en) * 2022-12-20 2024-07-11 Monsanto Technology Llc Transgenic corn event zm_csm63715 and methods for detection and uses thereof
PY2430924A (es) 2023-04-19 2025-02-17 Syngenta Crop Protection Ag Plantas resistentes a herbicidas
GB202314578D0 (en) 2023-09-22 2023-11-08 Univ Manchester Methods of producing homoplasmic modified plants or parts thereof
WO2025153595A1 (en) 2024-01-17 2025-07-24 Basf Se Plants having increased tolerance to herbicides

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UA126894C2 (uk) 2015-08-03 2023-02-22 Монсанто Текнолоджі Ллс Спосіб і композиція для стійкості рослин до гербіцидів
US10378023B2 (en) 2015-09-01 2019-08-13 Monsanto Technology Llc Methods and compositions for herbicide tolerance in plants
KR102587506B1 (ko) 2016-07-29 2023-10-11 몬산토 테크놀로지 엘엘씨 식물에서 유전자 발현을 위한 방법 및 조성물
CA3026528A1 (en) 2017-12-15 2019-06-15 Monsanto Technology Llc Methods and compositions for ppo herbicide tolerance

Also Published As

Publication number Publication date
PE20240226A1 (es) 2024-02-16
EP3394272A4 (en) 2019-07-31
RU2018124597A (ru) 2020-01-23
DK3394272T5 (da) 2024-09-30
JP2019500060A (ja) 2019-01-10
HRP20230311T1 (hr) 2023-05-12
KR102847177B1 (ko) 2025-08-20
AU2016377487A1 (en) 2018-06-28
US20170175131A1 (en) 2017-06-22
CO2018007568A2 (es) 2018-07-31
CN108463554A (zh) 2018-08-28
PH12018501317B1 (en) 2023-08-30
KR20180089518A (ko) 2018-08-08
CN108463554B (zh) 2023-04-18
RU2018124597A3 (es) 2020-05-28
ZA201803723B (en) 2019-07-31
AU2020281170B2 (en) 2021-05-20
RS64091B1 (sr) 2023-04-28
EP4219729A3 (en) 2023-09-27
BR102016029899A2 (pt) 2017-06-27
CN116355928A (zh) 2023-06-30
CA2950803A1 (en) 2017-06-21
PH12018501317A1 (en) 2019-02-18
MX2018007705A (es) 2018-11-09
AU2016377487B2 (en) 2020-12-24
EP4219729A2 (en) 2023-08-02
AU2020281170C1 (en) 2021-11-18
PL3394272T3 (pl) 2023-05-08
UY37040A (es) 2017-07-31
PT3394272T (pt) 2023-04-11
ES2941913T3 (es) 2023-05-26
JP7312805B2 (ja) 2023-07-21
US10563220B2 (en) 2020-02-18
EP3394272A1 (en) 2018-10-31
DK3394272T3 (da) 2023-04-03
PE20181540A1 (es) 2018-09-26
UA126554C2 (uk) 2022-11-02
UY40405A (es) 2023-09-29
AU2020281170A1 (en) 2021-02-04
WO2017112589A1 (en) 2017-06-29
EP3394272B1 (en) 2023-01-11
JP6961611B2 (ja) 2021-11-05
JP2022009102A (ja) 2022-01-14
RU2751238C2 (ru) 2021-07-12
MX385151B (es) 2025-03-14
HUE061632T2 (hu) 2023-07-28

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