EP0214043A1 - Vorrichtung und Verfahren zur künstlichen Befruchtung, zur Transplantation von Embryos in die Gebärmutter oder zur Entnahme von Flüssigkeiten durch das Bauchfell von Tieren - Google Patents

Vorrichtung und Verfahren zur künstlichen Befruchtung, zur Transplantation von Embryos in die Gebärmutter oder zur Entnahme von Flüssigkeiten durch das Bauchfell von Tieren Download PDF

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Publication number
EP0214043A1
EP0214043A1 EP86401825A EP86401825A EP0214043A1 EP 0214043 A1 EP0214043 A1 EP 0214043A1 EP 86401825 A EP86401825 A EP 86401825A EP 86401825 A EP86401825 A EP 86401825A EP 0214043 A1 EP0214043 A1 EP 0214043A1
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EP
European Patent Office
Prior art keywords
instrument
needle
catheter
instrument according
embryos
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP86401825A
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English (en)
French (fr)
Inventor
Yves Cognie
Jean Charles Edmond Vallet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Recherche Agronomique INRA
Original Assignee
Institut National de la Recherche Agronomique INRA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de la Recherche Agronomique INRA filed Critical Institut National de la Recherche Agronomique INRA
Publication of EP0214043A1 publication Critical patent/EP0214043A1/de
Withdrawn legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/027Devices for injecting semen into animals, e.g. syringes, guns, probes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/04Instruments or methods for reproduction or fertilisation for embryo transplantation

Definitions

  • the invention relates to a method and an instrument for artificial insemination, transplantation of intrauterine embryos or the puncture of follicular fluids, transperitoneally, in animals, in particular sheep, goats, cattle, horses or others.
  • sheep and goats we know the difficulties encountered during artificial insemination or the transfer of embryos by natural means.
  • the body of the uterus being difficult to cross in goats and, impassable in sheep, we were therefore forced, until then, to carry out a surgical intervention, under general anesthesia, with the drawbacks that this generates from the medical point of view and economical.
  • Such a surgical operation supposes, in fact, the existence of sophisticated equipment which is obviously not suited to the flexibility required for field techniques. To this investment is added anesthesia costs as well as post-operative care, which significantly increases the cost of the intervention.
  • the method according to the invention is characterized in that one makes in the abdominal wall of the animal, fine close incisions, one being intended to receive an endoscope making it possible to view the operating field, the other being intended the passage of a trocar provided with a guide sleeve, the withdrawal of this trocar, after perforation, allowing the passage, in the guide sleeve remaining in place, of an insemination, transplantation or follicular fluid removal instrument , presenting the originality of being connected to a suction means and also comprising, a injection syringe to allow on the one hand, the fixing, by suction effect, of the wall of one of the two uterine horns and, on the other hand, to cross the endometrium to bring the needle into the lumen of the horn to be treated, in order to deposit the semen or the embryos therein or to practice the desired samples, after which the second horn is treated, still under endoscopy, after withdrawal of the needle and new orientation of the instrument.
  • the instrument for implementing this process consists of a rigid probe formed by two separate coaxial conduits, one connected to a suction pump, the other serving as a guide for a punctate catheter whose base is radially slotted so that the operator can view the movement of the needle and in particular control its introduction into the lumen of the horn.
  • the catheter consists of a syringe comprising on the one hand a sheath, closed at one of its ends by an end piece extended by a needle, said end piece comprising a frustoconical internal cavity, on the other hand a sliding piston with seal in this sheath.
  • the catheter contains a fine straw for conditioning the semen or embryos, made integral with the tip by force fitting into its frustoconical cavity, the injection then being carried out by the sliding piston placed in support , by its front end, on the plug usually closing this type of glitter.
  • the essential object of the invention is to provide practitioners with flexible technology and instrumentation, of simple design, which are nevertheless of high reliability and at the lowest possible cost.
  • two incisions 1 and 2 are made on the abdomen of the animal (approximately in the vicinity of what is called the "white line” for sheep and goats and on the flank for cattle and horses). 2, fine and close together, and an endoscope 3 is introduced into one of them, making it possible to visualize the operating field.
  • the abdominal wall and the peritoneum are then punctured using a trocar, conventionally constituted by a mandrel (not shown) and its guide sleeve 4 which, after removal of the mandrel, will serve as a sheath for the introduction and guiding the specific instrument 5 of the invention.
  • this instrument is similar to a rigid probe comprising two tangential channels 51, 52 ( Figure 2), these being defined by two tubes 6 and 7, of different diameters, housed one in the other and joined at their two ends by brazing, the conduit 51 being separated from the conduit 52 and comprising a radial notch 53 for viewing the needle.
  • the external channel 52 is connected by connection 8 to a suction means, while the internal channel 51 serves as a guide for a punctiform catheter 9 formed essentially (FIG. 4) of a sheath 10, the base of which is closed by a nozzle. 11 extended by a needle 12, this sheath introduced into the channel 51 receiving a piston 13, calibrated using marks 13 de, the head of which slides with sealing in the inner duct of the sheath.
  • the catheter in the simplest version, in particular when injecting fresh seeds or immediately transplanting embryos, the catheter consists, as described above, of a simple needle sheath and a injection piston.
  • fine flakes 14 are used, which are fixed inside the catheter, and the closure plug 15 of which serves as a piston.
  • these fine flakes are used for the conservation, in cryogenic medium, of seeds or embryos and that they are formed of a capillary tube welded at one of its ends and closed at the other by a plug containing a powder. polymerizable on contact with the substance.
  • the anterior end must be cut off and forced into the conical cavity 16 of the catheter tip in order to immobilize it. It then suffices to use the piston 13, after having removed its removable head, so that the latter can slide in the fine straw and come into abutment on the plug 15 which then acts as the piston head.
  • the catheter contains two doses of fifteen million sperm each to treat the two horns 20.
  • a dose has been injected, it suffices to partially withdraw the catheter and in particular to needle 12, then tilt, under endoscopy, the instrument, to bring its front end facing the second horn, then repeat the operations.
  • the method and the instrument according to the invention have many advantages, because to the finesse of the surgery used and its safety, are added those linked to the flexibility and the economic nature of the intervention. Indeed, the simple design instrument makes it possible to treat animals directly in the field, since we no longer have recourse to the facilities specific to heavy surgery and general anesthesia. In addition, the incisions being fine, the animal is not very stressed and the post-operative care practically non-existent. Finally, and this is a particularly important advantage in the context of artificial insemination of sheep and goats, we can set up using this instrumentation, a low number of spermatozoa (thirty million for the two horns) where it was needed, with traditional techniques 400 million spermatozoa.
  • this instrument is particularly well suited to embryo transfer and the removal of follicular fluids from sheep, goats, cattle or horses, only the dimensions of the device changing according to the size of the body. 'animal.
  • this instrument is easy to handle because the operator can hold the endoscope and the probe with one hand, which includes a gripping ring 25 for this purpose.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Reproductive Health (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Transplantation (AREA)
  • Animal Husbandry (AREA)
  • Media Introduction/Drainage Providing Device (AREA)
EP86401825A 1985-08-19 1986-08-18 Vorrichtung und Verfahren zur künstlichen Befruchtung, zur Transplantation von Embryos in die Gebärmutter oder zur Entnahme von Flüssigkeiten durch das Bauchfell von Tieren Withdrawn EP0214043A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8512491 1985-08-19
FR8512491A FR2597328A1 (fr) 1985-08-19 1985-08-19 Instrument pour l'insemination artificielle, la transplantation d'embryons in utero ou le prelevement de liquides folliculaires, par voie transperitoneale, chez les animaux.

Publications (1)

Publication Number Publication Date
EP0214043A1 true EP0214043A1 (de) 1987-03-11

Family

ID=9322287

Family Applications (1)

Application Number Title Priority Date Filing Date
EP86401825A Withdrawn EP0214043A1 (de) 1985-08-19 1986-08-18 Vorrichtung und Verfahren zur künstlichen Befruchtung, zur Transplantation von Embryos in die Gebärmutter oder zur Entnahme von Flüssigkeiten durch das Bauchfell von Tieren

Country Status (7)

Country Link
EP (1) EP0214043A1 (de)
AU (1) AU6154286A (de)
BR (1) BR8603951A (de)
ES (1) ES2001100A6 (de)
FR (1) FR2597328A1 (de)
GR (1) GR862140B (de)
ZA (1) ZA866057B (de)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2603796A1 (fr) * 1986-09-17 1988-03-18 Agronomique Inst Nat Rech Instrumentation pour la collecte d'embryons, sous laparoscopie, chez les petits ruminants
FR2609885A1 (fr) * 1987-01-22 1988-07-29 Cassou Robert Instrument pour l'insemination artificielle, le transfert d'embryons ou le prelevement de liquides folliculaires chez les mammiferes
US6117068A (en) * 1995-10-19 2000-09-12 Elite Genetics, Inc Artificial insemination system
ES2156579A1 (es) * 2000-01-20 2001-06-16 Univ Murcia Dispositivo y metodo para introducir y/o recoger fluidos en el interior del utero de un animal.
WO2002028311A1 (en) * 2000-10-05 2002-04-11 Xy, Inc. System of hysteroscopic insemination of mares
EP2329793A2 (de) 2009-12-02 2011-06-08 German Genetics International GmbH Kathetervorrichtung zur künstlichen Befruchtung
EP2529695A2 (de) 2011-05-31 2012-12-05 German Genetics International GmbH Endoskop zum Gametentransfer
EP2353543A4 (de) * 2008-09-26 2014-04-09 Univ Murcia Vorrichtung und verfahren zur einführung oder gewinnung einer flüssigkeit mit keimzellen, embryonen oder einer anderen art von lösung im eileiter vion mutterschweinen
CN104546219A (zh) * 2015-01-22 2015-04-29 山东东阿阿胶股份有限公司 驴用输精枪及其使用方法
US9433484B2 (en) 2007-07-27 2016-09-06 Brad K. Stroud Artificial breeding techniques for bovines including semen diluents and AI apparatus
US9554883B2 (en) 2010-08-10 2017-01-31 Brad K. Stroud Method and apparatus to reduce the number of sperm used in artificial insemination of cattle
US10610343B2 (en) 2013-07-03 2020-04-07 Brad K. Stroud Method, apparatus and kit for artificial insemination of bovine
US11622844B2 (en) 2010-08-10 2023-04-11 Maximate, Llc Method, apparatus and kit for artificial insemination of bovine

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0905581B1 (pt) * 2009-12-17 2021-03-23 Empresa Brasileira De Pesquisa Agropecuária - Embrapa Circuito para coleta de embriões pela via transcervical em caprinos e ovinos
BRPI1004431A2 (pt) * 2010-06-09 2012-02-14 Embrapa sonda para coleta de embriões pela via transcervical em caprinos e ovinos
FR3036954B1 (fr) * 2015-06-03 2017-07-14 Imv Tech Paillette pour la conservation d'une dose predeterminee de substance a base liquide, ainsi que dispositif d'injection et procede la mettant en oeuvre

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1422490A (en) * 1921-03-26 1922-07-11 Stader Otto Veterinary surgical instrument
US3792703A (en) * 1972-07-10 1974-02-19 Deseret Pharma Catheter placement unit
WO1982001825A1 (en) * 1980-12-03 1982-06-10 Kevin W Gobby Apparatus for artificial insemination
WO1985000009A1 (en) * 1983-06-15 1985-01-03 Wiegerinck Martinus Antonius H Method and device to perforate, in particular to puncture a membranous member while utilizing vacuum fixation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1422490A (en) * 1921-03-26 1922-07-11 Stader Otto Veterinary surgical instrument
US3792703A (en) * 1972-07-10 1974-02-19 Deseret Pharma Catheter placement unit
WO1982001825A1 (en) * 1980-12-03 1982-06-10 Kevin W Gobby Apparatus for artificial insemination
WO1985000009A1 (en) * 1983-06-15 1985-01-03 Wiegerinck Martinus Antonius H Method and device to perforate, in particular to puncture a membranous member while utilizing vacuum fixation

Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2603796A1 (fr) * 1986-09-17 1988-03-18 Agronomique Inst Nat Rech Instrumentation pour la collecte d'embryons, sous laparoscopie, chez les petits ruminants
FR2609885A1 (fr) * 1987-01-22 1988-07-29 Cassou Robert Instrument pour l'insemination artificielle, le transfert d'embryons ou le prelevement de liquides folliculaires chez les mammiferes
EP0278823A1 (de) * 1987-01-22 1988-08-17 Robert Cassou Instrument zur künstlichen Besamung, zur Übertragung von Embryos oder zur Entnahme von follikulären Flüssigkeiten bei Säugetieren
US4846785A (en) * 1987-01-22 1989-07-11 Robert Cassou Instrument for artificial insemination, embryo transfer or sampling follicular liquids in mammals
US6117068A (en) * 1995-10-19 2000-09-12 Elite Genetics, Inc Artificial insemination system
ES2156579A1 (es) * 2000-01-20 2001-06-16 Univ Murcia Dispositivo y metodo para introducir y/o recoger fluidos en el interior del utero de un animal.
WO2001052767A1 (es) * 2000-01-20 2001-07-26 Universidad De Murcia Dispositivo y método para introducir y/o recoger fluidos en el interior del utero de un animal
AU776612B2 (en) * 2000-01-20 2004-09-16 Universidad De Murcia A device and method for introducing and/or collecting fluids in the inside of an animal uterus
WO2002028311A1 (en) * 2000-10-05 2002-04-11 Xy, Inc. System of hysteroscopic insemination of mares
GB2383543A (en) * 2000-10-05 2003-07-02 Xy Inc System of hysteroscopic insemination of mares
GB2383543B (en) * 2000-10-05 2005-03-09 Xy Inc System of hysteroscopic insemination of mares
US9433484B2 (en) 2007-07-27 2016-09-06 Brad K. Stroud Artificial breeding techniques for bovines including semen diluents and AI apparatus
US9277981B2 (en) 2008-09-26 2016-03-08 Universidad De Murcia Device and method for inserting or obtaining a fluid with gametes, embryos or any other type of solution in the oviduct of a sow
EP2353543A4 (de) * 2008-09-26 2014-04-09 Univ Murcia Vorrichtung und verfahren zur einführung oder gewinnung einer flüssigkeit mit keimzellen, embryonen oder einer anderen art von lösung im eileiter vion mutterschweinen
AU2009295755B2 (en) * 2008-09-26 2015-07-16 Universidad De Murcia Device and method for inserting or obtaining a fluid with gametes, embryos or any other type of solution in the oviduct of a sow
EP3263067A1 (de) * 2008-09-26 2018-01-03 Universidad de Murcia Vorrichtung zur einführung oder gewinnung einer flüssigkeit mit keimzellen, embryonen oder einer anderen art von lösung aus einer eileiter von mutterschweinen
DE102009047423A1 (de) 2009-12-02 2011-06-09 German Genetics International Gmbh Kathetervorrichtung
EP2329793A2 (de) 2009-12-02 2011-06-08 German Genetics International GmbH Kathetervorrichtung zur künstlichen Befruchtung
US9554883B2 (en) 2010-08-10 2017-01-31 Brad K. Stroud Method and apparatus to reduce the number of sperm used in artificial insemination of cattle
USRE48283E1 (en) 2010-08-10 2020-10-27 Brad Stroud Method and apparatus to reduce the number of sperm used in artificial insemination of cattle
US11622844B2 (en) 2010-08-10 2023-04-11 Maximate, Llc Method, apparatus and kit for artificial insemination of bovine
EP2529695A2 (de) 2011-05-31 2012-12-05 German Genetics International GmbH Endoskop zum Gametentransfer
DE102011076844A1 (de) 2011-05-31 2012-12-06 German Genetics International Gmbh Endoskop zum Gametentransfer
US10610343B2 (en) 2013-07-03 2020-04-07 Brad K. Stroud Method, apparatus and kit for artificial insemination of bovine
CN104546219A (zh) * 2015-01-22 2015-04-29 山东东阿阿胶股份有限公司 驴用输精枪及其使用方法

Also Published As

Publication number Publication date
GR862140B (en) 1986-12-30
FR2597328A1 (fr) 1987-10-23
ZA866057B (en) 1987-03-25
ES2001100A6 (es) 1988-04-16
BR8603951A (pt) 1987-03-24
AU6154286A (en) 1987-02-26

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Inventor name: VALLET, JEAN CHARLES EDMOND